Restriction map for
pFN21AE6392
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaWI BseAI BseRI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DdeI DraIII
DrdI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI TaqI
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccI 1201 GTCTAC GT^[AC][GT]AC
AflII 220 CTTAAG C^TTAAG
AspI 1000 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1108 GGACC G^G[AT]CC
BanI 25 GGCACC G^G[CT][GA]CC
BanII 486 GAGCTC G[GA]GC[CT]^C
BfaI 124 CTAG C^TAG
BfrI 220 CTTAAG C^TTAAG
BsaAI 1137 TACGTG [CT]AC^GT[GA]
BsgI 826 GTGCAG GTGCAG(16/14)
BsiEI 239 CGACCG CG[GA][CT]^CG
BsmBI 235 GAGACG CGTCTC(1/5)
BspMI 828 GCAGGT ACCTGC(4/8)
BsrBI 792 CCGCTC CCGCTC(-3/-3)
DraI 163 TTTAAA TTT^AAA
DraII 1107 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 49 CCATGG C^C[GA][CT]GG
EarI 834 CTCTTC CTCTTC(1/4)
EcoO109I 1107 GGGACCT [GA]G^G[ACGT]CC[CT]
HindIII 602 AAGCTT A^AGCTT
HpaI 504 GTTAAC GTT^AAC
Ksp632I 834 CTCTTC CTCTTC(1/4)
MaeI 124 CTAG C^TAG
NcoI 49 CCATGG C^CATGG
PflFI 1000 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1107 GGGACCT [GA]G^G[AT]CC[CT]
SacI 486 GAGCTC GAGCT^C
SbfI 1088 CCTGCAGG CCTGCA^GG
ScaI 146 AGTACT AGT^ACT
SmlI 220 CTTAAG C^T[CT][GA]AG
Sse8387I 1088 CCTGCAGG CCTGCA^GG
Tth111I 1000 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 29 enzymes) #
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AciI 792 CCGC CCGC(-3/-1)
586 GCGG
AhdI 99 GACCATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1109 GACCTTTAGTC
AlwI 175 GATCC GGATC(4/5)
1125 GGATC
AspEI 99 GACCATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1109 GACCTTTAGTC
AspHI 486 GAGCTC G[AT]GC[AT]^C
935 GAGCAC
BbvI 673 GCAGC GCAGC(8/12)
799 GCAGC
BmyI 486 GAGCTC G[AGT]GC[ACT]^C
935 GAGCAC
BsaI 106 GGTCTC GGTCTC(1/5)
831 GGTCTC
BsiHKAI 486 GAGCTC G[AT]GC[AT]^C
935 GAGCAC
BsmFI 1107 GGGAC GGGAC(10/14)
78 GTCCC
Bsp1286I 486 GAGCTC G[AGT]GC[ACT]^C
935 GAGCAC
BsrI 1229 ACTGG ACTGG(1/-1)
363 CCAGT
BstXI 627 CCATCAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
703 CCACATCAATGG
BstYI 174 AGATCC [GA]^GATC[CT]
1125 GGATCT
Cfr13I 638 GGGCC G^G[ACGT]CC
1108 GGACC
Eam1105I 99 GACCATAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1109 GACCTTTAGTC
HapII 867 CCGG C^CGG
1061 CCGG
HincII 504 GTTAAC GT[CT]^[GA]AC
550 GTCAAC
HindII 504 GTTAAC GT[CT]^[GA]AC
550 GTCAAC
HpaII 867 CCGG C^CGG
1061 CCGG
HphI 1096 GGTGA GGTGA(8/7)
845 TCACC
MaeII 548 ACGT A^CGT
1138 ACGT
MflI 174 AGATCC [GA]^GATC[CT]
1125 GGATCT
MspI 867 CCGG C^CGG
1061 CCGG
MwoI 26 GCACCCGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
577 GCACCCAAGGC
Sau96I 638 GGGCC G^G[ACGT]CC
1108 GGACC
TseI 673 GCAGC G^C[AT]GC
799 GCAGC
XcmI 101 CCATAGGTCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
513 CCAAGCAAGTTGTGG
XhoII 174 AGATCC [GA]^GATC[CT]
1125 GGATCT
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AcsI AfaI AluI ApoI BpmI
BsaJI BsiYI BslI BsmAI BsrDI
BssKI BstF5I BstNI Cac8I DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI ItaI MaeIII MboI MboII
MnlI MseI MslI MvaI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI ScrFI SfaNI SfcI StyI
TfiI Tru9I Tsp45I Tsp509I TspRI