(Linear) (Six-base) MAPSORT of: KIEE6392.seq Check: 78 from: 1 to: 1254 KIEE6392.orf With 182 enzymes: SgfI * March 16, 2015 11:30 .. AccI GT'mk_AC Cuts at: 0 1202 1254 Size: 1202 52 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 595 1254 Size: 64 531 659 Fragments arranged by size: 659 531 64 AflII C'TTAA_G Cuts at: 0 220 1254 Size: 220 1034 AhdI GACnn_n'nnGTC Cuts at: 0 104 1114 1254 Size: 104 1010 140 Fragments arranged by size: 1010 140 104 AleI CACnn'nnGTG Cuts at: 0 1210 1254 Size: 1210 44 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 696 1254 Size: 696 558 ApoI r'AATT_y Cuts at: 0 167 246 435 906 1076 1254 Size: 167 79 189 471 170 178 Fragments arranged by size: 471 189 178 170 167 79 BanI G'GyrC_C Cuts at: 0 25 1254 Size: 25 1229 BanII G_rGCy'C Cuts at: 0 490 1254 Size: 490 764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 305 587 1217 1254 Size: 305 282 630 37 Fragments arranged by size: 630 305 282 37 BsaI GGTCTCn'nnnn_ Cuts at: 0 112 837 1254 Size: 112 725 417 Fragments arranged by size: 725 417 112 BsaAI yAC'GTr Cuts at: 0 1139 1254 Size: 1139 115 BseYI C'CCAG_C Cuts at: 0 1163 1254 Size: 1163 91 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 847 1254 Size: 847 407 BsiEI CG_ry'CG Cuts at: 0 242 1254 Size: 242 1012 BsiHKAI G_wGCw'C Cuts at: 0 490 939 1254 Size: 490 449 315 Fragments arranged by size: 490 449 315 BsmBI CGTCTCn'nnnn_ Cuts at: 0 229 1254 Size: 229 1025 Bsp1286I G_dGCh'C Cuts at: 0 490 939 1254 Size: 490 449 315 Fragments arranged by size: 490 449 315 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 819 1254 Size: 819 435 BsrBI CCG'CTC Cuts at: 0 794 1254 Size: 794 460 BsrDI GCAATG_nn' Cuts at: 0 488 572 815 1038 1254 Size: 488 84 243 223 216 Fragments arranged by size: 488 243 223 216 84 BstXI CCAn_nnnn'nTGG Cuts at: 0 634 710 1254 Size: 634 76 544 Fragments arranged by size: 634 544 76 BstYI r'GATC_y Cuts at: 0 174 1125 1254 Size: 174 951 129 Fragments arranged by size: 951 174 129 BtgI C'CryG_G Cuts at: 0 49 1254 Size: 49 1205 BtsI GCAGTG_nn' Cuts at: 0 101 668 678 1254 Size: 101 567 10 576 Fragments arranged by size: 576 567 101 10 DraI TTT'AAA Cuts at: 0 165 1254 Size: 165 1089 EarI CTCTTCn'nnn_ Cuts at: 0 840 1254 Size: 840 414 EcoICRI GAG'CTC Cuts at: 0 488 1254 Size: 488 766 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 305 587 595 1217 1254 Size: 64 241 282 8 622 37 Fragments arranged by size: 622 282 241 64 37 8 EcoO109I rG'GnC_Cy Cuts at: 0 1108 1254 Size: 1108 146 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 625 1254 Size: 625 629 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 90 122 761 793 974 1006 1100 1132 Size: 90 32 639 32 181 32 94 32 Cuts at: 1132 1254 Size: 122 Fragments arranged by size: 639 181 122 94 90 32 32 32 32 HincII GTy'rAC Cuts at: 0 506 552 1254 Size: 506 46 702 Fragments arranged by size: 702 506 46 HindIII A'AGCT_T Cuts at: 0 602 1254 Size: 602 652 HpaI GTT'AAC Cuts at: 0 506 1254 Size: 506 748 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 924 1254 Size: 924 330 MslI CAynn'nnrTG Cuts at: 0 355 632 708 1210 1254 Size: 355 277 76 502 44 Fragments arranged by size: 502 355 277 76 44 NcoI C'CATG_G Cuts at: 0 49 1254 Size: 49 1205 PpuMI rG'GwC_Cy Cuts at: 0 1108 1254 Size: 1108 146 PstI C_TGCA'G Cuts at: 0 675 801 1093 1254 Size: 675 126 292 161 Fragments arranged by size: 675 292 161 126 SacI G_AGCT'C Cuts at: 0 490 1254 Size: 490 764 SbfI CC_TGCA'GG Cuts at: 0 1093 1254 Size: 1093 161 ScaI AGT'ACT Cuts at: 0 148 1254 Size: 148 1106 SfcI C'TryA_G Cuts at: 0 671 797 1089 1254 Size: 671 126 292 165 Fragments arranged by size: 671 292 165 126 SmlI C'TyrA_G Cuts at: 0 220 1254 Size: 220 1034 StyI C'CwwG_G Cuts at: 0 49 581 595 1254 Size: 49 532 14 659 Fragments arranged by size: 659 532 49 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 256 594 1254 Size: 256 338 660 Fragments arranged by size: 660 338 256 TatI w'GTAC_w Cuts at: 0 146 1254 Size: 146 1108 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 668 700 1254 Size: 668 32 554 Fragments arranged by size: 668 554 32 Tth111I GACn'n_nGTC Cuts at: 0 1003 1254 Size: 1003 251 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 108 520 1254 Size: 108 412 734 Fragments arranged by size: 734 412 108 Enzymes that do cut: AccI AcuI AflII AhdI AleI AlfI ApoI BanI BanII BpmI BsaI BsaAI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BstXI BstYI BtgI BtsI DraI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HincII HindIII HpaI MmeI MslI NcoI PpuMI PstI SacI SbfI ScaI SfcI SmlI StyI TaqII TatI TstI Tth111I XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflIII AgeI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaBI BsaHI BsaWI BsaXI BseRI BsiWI BsmI BspEI BspHI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII KasI KpnI MfeI MluI MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SapI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XhoI XmaI XmnI ZraI