(Circular) (Six-base) MAPSORT of: pFN21AE9538.seq Check: 9236 from: 1 to: 5689 pFN21AE9538 5689 bp With 182 enzymes: SgfI * March 28, 2012 12:01 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3499 278 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3032 1937 Size: 1095 4594 Acc65I G'GTAC_C Cuts at: 3010 3010 Size: 5689 AclI AA'CG_TT Cuts at: 1563 3816 4189 1563 Size: 2253 373 3063 Fragments arranged by size: 3063 2253 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2168 2521 2635 2813 3752 5136 5404 1632 Size: 536 353 114 178 939 1384 268 1917 Fragments arranged by size: 1917 1384 939 536 353 268 178 114 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5492 AflIII A'CryG_T Cuts at: 1203 4588 5565 1203 Size: 3385 977 1327 Fragments arranged by size: 3385 1327 977 AgeI A'CCGG_T Cuts at: 4520 4520 Size: 5689 AhdI GACnn_n'nnGTC Cuts at: 4418 4418 Size: 5689 AleI CACnn'nnGTG Cuts at: 1166 2617 1166 Size: 1451 4238 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5689 AlwNI CAG_nnn'CTG Cuts at: 3432 5004 3432 Size: 1572 4117 ApaLI G'TGCA_C Cuts at: 3746 4902 3746 Size: 1156 4533 ApoI r'AATT_y Cuts at: 1493 2865 2998 3224 3376 5485 1493 Size: 1372 133 226 152 2109 1697 Fragments arranged by size: 2109 1697 1372 226 152 133 AseI AT'TA_AT Cuts at: 160 1032 4243 160 Size: 872 3211 1606 Fragments arranged by size: 3211 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 AvaI C'yCGr_G Cuts at: 1870 1943 3014 1870 Size: 73 1071 4545 Fragments arranged by size: 4545 1071 73 AvrII C'CTAG_G Cuts at: 2860 2860 Size: 5689 BamHI G'GATC_C Cuts at: 2496 3019 3386 2496 Size: 523 367 4799 Fragments arranged by size: 4799 523 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3010 4465 618 Size: 325 217 627 1223 1455 1842 Fragments arranged by size: 1842 1455 1223 627 325 217 BanII G_rGCy'C Cuts at: 1391 3008 1391 Size: 1617 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2801 928 Size: 971 902 3816 Fragments arranged by size: 3816 971 902 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3880 3914 1903 Size: 34 1943 34 3678 Fragments arranged by size: 3678 1943 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3581 4791 3581 Size: 1210 4479 BclI T'GATC_A Cuts at: 1548 2406 1548 Size: 858 4831 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4300 136 Size: 107 122 71 631 605 2628 1525 Fragments arranged by size: 2628 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5684 5684 Size: 5689 BlpI GC'TnA_GC Cuts at: 3106 3106 Size: 5689 Bme1580I G_kGCm'C Cuts at: 1790 3750 4906 1790 Size: 1960 1156 2573 Fragments arranged by size: 2573 1960 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5689 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2906 3420 4378 Size: 416 53 83 399 24 1489 514 958 Cuts at: 4378 442 Size: 1753 Fragments arranged by size: 1753 1489 958 514 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5689 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2357 2357 Size: 5689 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2352 4349 1239 Size: 1113 1997 2579 Fragments arranged by size: 2579 1997 1113 Bpu10I CC'TnA_GC Cuts at: 2257 2257 Size: 5689 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2209 3814 4679 4977 2209 Size: 1605 865 298 2921 Fragments arranged by size: 2921 1605 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4352 882 Size: 617 2853 2219 Fragments arranged by size: 2853 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3480 493 Size: 711 2276 2702 Fragments arranged by size: 2702 2276 711 BsaBI GATnn'nnATC Cuts at: 5683 5683 Size: 5689 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3496 3878 4513 275 Size: 53 83 186 936 1963 382 635 1451 Fragments arranged by size: 1963 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4122 4520 4794 4941 5352 4122 Size: 398 274 147 411 4459 Fragments arranged by size: 4459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5464 Fragments arranged by size: 5464 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5689 BseYI C'CCAG_C Cuts at: 2386 4892 5388 2386 Size: 2506 496 2687 Fragments arranged by size: 2687 2506 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5116 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3139 3900 4928 1540 Size: 275 186 1138 761 1028 2301 Fragments arranged by size: 2301 1138 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2788 3750 3835 4906 2788 Size: 962 85 1071 3571 Fragments arranged by size: 3571 1071 962 85 BsmI GAATG_Cn' Cuts at: 3200 3293 3200 Size: 93 5596 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2788 3008 3750 3835 4906 1391 Size: 399 998 220 742 85 1071 2174 Fragments arranged by size: 2174 1071 998 742 399 220 85 BspEI T'CCGG_A Cuts at: 5352 5352 Size: 5689 BspHI T'CATG_A Cuts at: 2394 3578 5308 2394 Size: 1184 1730 2775 Fragments arranged by size: 2775 1730 1184 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3044 844 Size: 2200 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2421 3576 1394 Size: 429 598 1155 3507 Fragments arranged by size: 3507 1155 598 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2701 4184 4358 65 Size: 1183 1453 1483 174 1396 Fragments arranged by size: 1483 1453 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4333 4520 1700 Size: 110 2523 187 2869 Fragments arranged by size: 2869 2523 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5689 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5689 BssSI C'ACGA_G Cuts at: 2434 3749 4761 2434 Size: 1315 1012 3362 Fragments arranged by size: 3362 1315 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2015 794 Size: 1221 4468 BstBI TT'CG_AA Cuts at: 4493 4493 Size: 5689 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5689 BstXI CCAn_nnnn'nTGG Cuts at: 2269 2269 Size: 5689 BstYI r'GATC_y Cuts at: 1972 2112 2496 3019 3386 3771 3788 5240 5326 Size: 140 384 523 367 385 17 1452 86 Cuts at: 5326 5338 5684 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 523 385 384 367 346 140 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2478 513 Size: 552 939 474 3724 Fragments arranged by size: 3724 939 552 474 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4950 Fragments arranged by size: 4950 683 56 BtsI GCAGTG_nn' Cuts at: 2599 3205 3999 4019 2599 Size: 606 794 20 4269 Fragments arranged by size: 4269 794 606 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5689 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5654 DraI TTT'AAA Cuts at: 2946 2993 2946 Size: 47 5642 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4696 809 Size: 507 3380 1802 Fragments arranged by size: 3380 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3136 4025 5654 Size: 54 1425 50 275 18 1306 889 1629 Cuts at: 5654 8 Size: 43 Fragments arranged by size: 1629 1425 1306 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3136 1537 Size: 275 1324 4090 Fragments arranged by size: 4090 1324 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2666 3619 1353 Size: 1313 953 3423 Fragments arranged by size: 3423 1313 953 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4276 4650 4796 387 Size: 1064 138 2687 374 146 1280 Fragments arranged by size: 2687 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2352 2521 2635 2813 3752 4349 Size: 393 536 184 169 114 178 939 597 Cuts at: 4349 5136 5404 1239 Size: 787 268 1524 Fragments arranged by size: 1524 939 787 597 536 393 268 184 178 169 114 EcoNI CCTnn'n_nnAGG Cuts at: 2732 2732 Size: 5689 EcoO109I rG'GnC_Cy Cuts at: 2439 2725 2737 2439 Size: 286 12 5391 Fragments arranged by size: 5391 286 12 EcoRI G'AATT_C Cuts at: 2998 3376 2998 Size: 378 5311 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2257 2257 Size: 5689 FspI TGC'GCA Cuts at: 4195 4195 Size: 5689 HaeII r_GCGC'y Cuts at: 1678 4836 1678 Size: 3158 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2533 2565 4330 4362 4404 Size: 32 135 32 961 32 1765 32 42 Cuts at: 4404 4436 1373 Size: 32 2626 Fragments arranged by size: 2626 1765 961 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3033 3279 669 Size: 988 281 1095 246 3079 Fragments arranged by size: 3079 1095 988 281 246 HindIII A'AGCT_T Cuts at: 748 2366 748 Size: 1618 4071 HpaI GTT'AAC Cuts at: 3279 3279 Size: 5689 KpnI G_GTAC'C Cuts at: 3014 3014 Size: 5689 MfeI C'AATT_G Cuts at: 3288 3288 Size: 5689 MluI A'CGCG_T Cuts at: 5565 5565 Size: 5689 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2250 2449 4803 4987 1305 Size: 945 199 2354 184 2007 Fragments arranged by size: 2354 2007 945 199 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3867 Fragments arranged by size: 3867 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2399 2617 3647 4006 4165 518 Size: 648 1233 218 1030 359 159 2042 Fragments arranged by size: 2042 1233 1030 648 359 218 159 MspA1I CmG'CkG Cuts at: 1606 2655 3105 3782 4930 5175 1606 Size: 1049 450 677 1148 245 2120 Fragments arranged by size: 2120 1148 1049 677 450 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2478 513 Size: 552 939 474 3724 Fragments arranged by size: 3724 939 552 474 NdeI CA'TA_TG Cuts at: 387 387 Size: 5689 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5689 NotI GC'GGCC_GC Cuts at: 3136 3136 Size: 5689 NspI r_CATG'y Cuts at: 1132 1229 3047 4592 1132 Size: 97 1818 1545 2229 Fragments arranged by size: 2229 1818 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5689 PciI A'CATG_T Cuts at: 4588 4588 Size: 5689 PflMI CCAn_nnn'nTGG Cuts at: 1108 2297 1108 Size: 1189 4500 PmeI GTTT'AAAC Cuts at: 2993 2993 Size: 5689 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3758 3790 5271 5303 3758 Size: 32 1481 32 4144 Fragments arranged by size: 4144 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2725 2737 2725 Size: 12 5677 PshAI GACnn'nnGTC Cuts at: 1613 2723 1613 Size: 1110 4579 PsiI TTA'TAA Cuts at: 2637 3259 2637 Size: 622 5067 PstI C_TGCA'G Cuts at: 830 2606 3041 830 Size: 1776 435 3478 Fragments arranged by size: 3478 1776 435 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5689 PvuII CAG'CTG Cuts at: 2655 2655 Size: 5689 SalI G'TCGA_C Cuts at: 1936 3031 1936 Size: 1095 4594 SbfI CC_TGCA'GG Cuts at: 3041 3041 Size: 5689 ScaI AGT'ACT Cuts at: 3937 5649 3937 Size: 1712 3977 SfcI C'TryA_G Cuts at: 826 1045 2602 3037 4172 4853 5044 826 Size: 219 1557 435 1135 681 191 1471 Fragments arranged by size: 1557 1471 1135 681 435 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5689 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5689 SmaI CCC'GGG Cuts at: 1872 3016 1872 Size: 1144 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2224 3793 4694 4956 820 Size: 197 926 281 1569 901 262 1553 Fragments arranged by size: 1569 1553 926 901 281 262 197 SnaBI TAC'GTA Cuts at: 493 3480 493 Size: 2987 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5689 SphI G_CATG'C Cuts at: 1132 3047 1132 Size: 1915 3774 SspI AAT'ATT Cuts at: 5 52 3613 5 Size: 47 3561 2081 Fragments arranged by size: 3561 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5689 StyI C'CwwG_G Cuts at: 513 1065 2004 2478 2860 3128 513 Size: 552 939 474 382 268 3074 Fragments arranged by size: 3074 939 552 474 382 268 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3716 3733 3886 4071 4516 325 Size: 1255 2136 17 153 185 445 1498 Fragments arranged by size: 2136 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3935 5647 96 Size: 274 80 33 51 1443 1958 1712 138 Fragments arranged by size: 1958 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2288 2320 5649 5681 2288 Size: 32 3329 32 2296 Fragments arranged by size: 3329 2296 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2101 1316 Size: 785 4904 XbaI T'CTAG_A Cuts at: 3025 3025 Size: 5689 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2327 2485 2327 Size: 158 5531 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5689 XmaI C'CCGG_G Cuts at: 1870 3014 1870 Size: 1144 4545 XmnI GAAnn'nnTTC Cuts at: 3818 3818 Size: 5689 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3497 276 Size: 53 83 186 936 1963 2468 Fragments arranged by size: 2468 1963 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI