Restriction map for
pFN21AE5418
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BpmI BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AfaI 486 GTAC GT^AC
AvaII 141 GGACC G^G[AT]CC
BamHI 722 GGATCC G^GATCC
BanI 275 GGTGCC G^G[CT][GA]CC
BlpI 531 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 531 GCTGAGC GC^T[ACGT]AGC
BsaBI 624 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmFI 211 GTCCC GGGAC(10/14)
BspHI 467 TCATGA T^CATGA
BsrI 737 ACTGG ACTGG(1/-1)
BssHII 45 GCGCGC G^CGCGC
BstXI 606 CCATATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 722 GGATCC [GA]^GATC[CT]
CelII 531 GCTGAGC GC^T[ACGT]AGC
DraIII 475 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 794 CCATGG C^C[GA][CT]GG
EaeI 797 TGGCCG [CT]^GGCC[GA]
EarI 430 CTCTTC CTCTTC(1/4)
EcoRI 338 GAATTC G^AATTC
EcoT14I 794 CCATGG C^C[AT][AT]GG
HaeII 30 GGCGCT [GA]GCGC^[CT]
HindIII 932 AAGCTT A^AGCTT
Ksp632I 430 CTCTTC CTCTTC(1/4)
MamI 624 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 722 GGATCC [GA]^GATC[CT]
MslI 243 CATCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 40 CCGCTG C[AC]G^C[GT]G
NcoI 794 CCATGG C^CATGG
PflMI 544 CCAATGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 428 GACTC GAGTC(4/5)
PpuMI 140 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 843 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 94 CTGCAG CTGCA^G
RcaI 467 TCATGA T^CATGA
RsaI 486 GTAC GT^AC
ScaI 485 AGTACT AGT^ACT
SfaNI 588 GATGC GCATC(5/9)
SfcI 94 CTGCAG C^T[GA][CT]AG
SmaI 120 CCCGGG CCC^GGG
StyI 794 CCATGG C^C[AT][AT]GG
TaqI 434 TCGA T^CGA
TfiI 471 GATTC G^A[AT]TC
TthHB8I 434 TCGA T^CGA
Van91I 544 CCAATGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 727 CCATTTGGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 722 GGATCC [GA]^GATC[CT]
XmaCI 120 CCCGGG C^CCGGG
XmaI 120 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 338 GAATTC [GA]^AATT[CT]
904 AAATTT
ApoI 338 GAATTC [GA]^AATT[CT]
904 AAATTT
Asp700I 258 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
930 GAAAGCTTTC
AspHI 22 GAGCAC G[AT]GC[AT]^C
302 GAGCAC
AvaI 120 CCCGGG C^[CT]CG[GA]G
343 CTCGGG
BfaI 397 CTAG C^TAG
835 CTAG
BmyI 22 GAGCAC G[AGT]GC[ACT]^C
302 GAGCAC
BsgI 412 CTGCAC GTGCAG(16/14)
481 GTGCAG
BsiHKAI 22 GAGCAC G[AT]GC[AT]^C
302 GAGCAC
BsmAI 734 GAGAC GTCTC(1/5)
910 GTCTC
BsmI 558 GAATGC GAATGC(1/-1)
385 GCATTC
BsoBI 120 CCCGGG C^[CT]CG[GA]G
343 CTCGGG
Bsp1286I 22 GAGCAC G[AGT]GC[ACT]^C
302 GAGCAC
BstAPI 96 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
376 GCACTGTCTGC
BstF5I 549 GGATG GGATG(2/0)
617 GGATG
Cfr13I 141 GGACC G^G[ACGT]CC
864 GGCCC
DraII 140 AGGACCT [GA]G^G[ACGT]CC[CT]
863 AGGCCCT
EcoO109I 140 AGGACCT [GA]G^G[ACGT]CC[CT]
863 AGGCCCT
FauI 39 CCCGC CCCGC(4/6)
187 CCCGC
FokI 549 GGATG GGATG(9/13)
617 GGATG
HapII 110 CCGG C^CGG
121 CCGG
HinfI 428 GACTC G^A[ACGT]TC
471 GATTC
HpaII 110 CCGG C^CGG
121 CCGG
HphI 840 GGTGA GGTGA(8/7)
803 TCACC
MaeI 397 CTAG C^TAG
835 CTAG
MseI 823 TTAA T^TAA
857 TTAA
MspI 110 CCGG C^CGG
121 CCGG
Sau96I 141 GGACC G^G[ACGT]CC
864 GGCCC
Tru9I 823 TTAA T^TAA
857 TTAA
Tsp45I 802 GTCAC ^GT[GC]AC
841 GTGAC
XmnI 258 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
930 GAAAGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
BbsI BbvI BcnI BpuAI BsaJI
BsiYI BslI BssKI BstNI BstUI
Cac8I CfoI DdeI DpnII EcoRII
Fnu4HI HaeIII HhaI HinP1I ItaI
MaeII MaeIII MboI MboII MnlI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV Sau3AI ScrFI TseI
Tsp509I TspRI