(Circular) (Six-base) MAPSORT of: pFN21AE0969.seq Check: 3763 from: 1 to: 6286 pFN21AE0969 6286 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4096 278 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3004 3629 1937 Size: 1067 625 4594 Fragments arranged by size: 4594 1067 625 Acc65I G'GTAC_C Cuts at: 2454 3607 2454 Size: 1153 5133 AclI AA'CG_TT Cuts at: 1563 4413 4786 1563 Size: 2850 373 3063 Fragments arranged by size: 3063 2850 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2268 2946 3330 3436 3465 3546 4349 5733 Size: 636 678 384 106 29 81 803 1384 Cuts at: 5733 6001 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 803 678 636 384 268 106 81 29 AflII C'TTAA_G Cuts at: 820 1017 2311 820 Size: 197 1294 4795 Fragments arranged by size: 4795 1294 197 AflIII A'CryG_T Cuts at: 1203 5185 6162 1203 Size: 3982 977 1327 Fragments arranged by size: 3982 1327 977 AgeI A'CCGG_T Cuts at: 5117 5117 Size: 6286 AhdI GACnn_n'nnGTC Cuts at: 5015 5015 Size: 6286 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6286 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6286 AlwNI CAG_nnn'CTG Cuts at: 3510 4029 5601 3510 Size: 519 1572 4195 Fragments arranged by size: 4195 1572 519 ApaLI G'TGCA_C Cuts at: 4343 5499 4343 Size: 1156 5130 ApoI r'AATT_y Cuts at: 1493 3219 3418 3595 3821 3973 6082 1493 Size: 1726 199 177 226 152 2109 1697 Fragments arranged by size: 2109 1726 1697 226 199 177 152 AseI AT'TA_AT Cuts at: 160 1032 4840 160 Size: 872 3808 1606 Fragments arranged by size: 3808 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 AvaI C'yCGr_G Cuts at: 1870 1943 3611 1870 Size: 73 1668 4545 Fragments arranged by size: 4545 1668 73 BamHI G'GATC_C Cuts at: 2549 3616 3983 2549 Size: 1067 367 4852 Fragments arranged by size: 4852 1067 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2454 3475 3607 5062 618 Size: 325 217 627 667 1021 132 1455 1842 Fragments arranged by size: 1842 1455 1021 667 627 325 217 132 BanII G_rGCy'C Cuts at: 1391 3605 1391 Size: 2214 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5315 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4477 4511 1903 Size: 34 2540 34 3678 Fragments arranged by size: 3678 2540 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4178 5388 4178 Size: 1210 5076 BclI T'GATC_A Cuts at: 1548 1548 Size: 6286 BfrBI ATG'CAT Cuts at: 3210 3210 Size: 6286 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4897 136 Size: 107 122 71 631 605 3225 1525 Fragments arranged by size: 3225 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6281 6281 Size: 6286 BlpI GC'TnA_GC Cuts at: 2153 3703 2153 Size: 1550 4736 Bme1580I G_kGCm'C Cuts at: 1790 4347 5503 1790 Size: 2557 1156 2573 Fragments arranged by size: 2573 2557 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6286 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4017 4975 442 Size: 416 53 83 399 24 2600 958 1753 Fragments arranged by size: 2600 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6286 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2360 3167 4946 1239 Size: 1121 807 1779 2579 Fragments arranged by size: 2579 1779 1121 807 Bpu10I CC'TnA_GC Cuts at: 2099 3089 2099 Size: 990 5296 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4411 5276 5574 4411 Size: 865 298 5123 Fragments arranged by size: 5123 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2367 2720 4949 882 Size: 617 868 353 2229 2219 Fragments arranged by size: 2229 2219 868 617 353 BsaAI yAC'GTr Cuts at: 493 1204 4077 493 Size: 711 2873 2702 Fragments arranged by size: 2873 2702 711 BsaBI GATnn'nnATC Cuts at: 6280 6280 Size: 6286 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4093 4475 5110 275 Size: 53 83 186 936 2560 382 635 1451 Fragments arranged by size: 2560 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4719 5117 5391 5538 5949 4719 Size: 398 274 147 411 5056 Fragments arranged by size: 5056 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6061 Fragments arranged by size: 6061 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6286 BseYI C'CCAG_C Cuts at: 2133 2774 5489 5985 2133 Size: 641 2715 496 2434 Fragments arranged by size: 2715 2434 641 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2087 2216 1163 Size: 573 351 129 5233 Fragments arranged by size: 5233 573 351 129 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3736 4497 5525 1540 Size: 275 186 1735 761 1028 2301 Fragments arranged by size: 2301 1735 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2105 4347 4432 5503 2105 Size: 2242 85 1071 2888 Fragments arranged by size: 2888 2242 1071 85 BsmI GAATG_Cn' Cuts at: 3797 3890 3797 Size: 93 6193 BsmBI CGTCTCn'nnnn_ Cuts at: 2732 2732 Size: 6286 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2105 3605 4347 4432 5503 1391 Size: 399 315 1500 742 85 1071 2174 Fragments arranged by size: 2174 1500 1071 742 399 315 85 BspEI T'CCGG_A Cuts at: 5949 5949 Size: 6286 BspHI T'CATG_A Cuts at: 2718 4175 5905 2718 Size: 1457 1730 3099 Fragments arranged by size: 3099 1730 1457 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3641 844 Size: 2797 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4173 1394 Size: 429 2350 3507 Fragments arranged by size: 3507 2350 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4781 4955 65 Size: 1183 3533 174 1396 Fragments arranged by size: 3533 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2711 4930 5117 1700 Size: 110 901 2219 187 2869 Fragments arranged by size: 2869 2219 901 187 110 BsrGI T'GTAC_A Cuts at: 96 3501 96 Size: 3405 2881 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6286 BssSI C'ACGA_G Cuts at: 4346 5358 4346 Size: 1012 5274 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6286 BstBI TT'CG_AA Cuts at: 5090 5090 Size: 6286 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6286 BstXI CCAn_nnnn'nTGG Cuts at: 3297 3297 Size: 6286 BstYI r'GATC_y Cuts at: 1972 2256 2549 2803 3616 3983 4368 4385 5837 Size: 284 293 254 813 367 385 17 1452 Cuts at: 5837 5923 5935 6281 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 813 385 367 346 293 284 254 86 17 12 BstZ17I GTA'TAC Cuts at: 3005 3005 Size: 6286 BtgI C'CryG_G Cuts at: 513 1065 2147 513 Size: 552 1082 4652 Fragments arranged by size: 4652 1082 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2123 487 Size: 683 56 897 4650 Fragments arranged by size: 4650 897 683 56 BtsI GCAGTG_nn' Cuts at: 2216 3802 4596 4616 2216 Size: 1586 794 20 3886 Fragments arranged by size: 3886 1586 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6286 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6251 DraI TTT'AAA Cuts at: 3590 3590 Size: 6286 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5293 809 Size: 507 3977 1802 Fragments arranged by size: 3977 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3733 4622 6251 Size: 54 1425 50 275 18 1903 889 1629 Cuts at: 6251 8 Size: 43 Fragments arranged by size: 1903 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3733 1537 Size: 275 1921 4090 Fragments arranged by size: 4090 1921 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2106 2244 2922 2937 4216 1353 Size: 753 138 678 15 1279 3423 Fragments arranged by size: 3423 1279 753 678 138 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4873 5247 5393 387 Size: 1064 138 3284 374 146 1280 Fragments arranged by size: 3284 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2268 2360 2946 3167 3330 3436 3465 Size: 393 636 92 586 221 163 106 29 Cuts at: 3465 3546 4349 4946 5733 6001 1239 Size: 81 803 597 787 268 1524 Fragments arranged by size: 1524 803 787 636 597 586 393 268 221 163 106 92 81 29 EcoRI G'AATT_C Cuts at: 3595 3973 3595 Size: 378 5908 EcoRV GAT'ATC Cuts at: 2735 2735 Size: 6286 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3104 3335 3104 Size: 231 6055 FspI TGC'GCA Cuts at: 4792 4792 Size: 6286 HaeII r_GCGC'y Cuts at: 1678 5433 1678 Size: 3755 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3338 3370 4927 4959 5001 Size: 32 135 32 1766 32 1557 32 42 Cuts at: 5001 5033 1373 Size: 32 2626 Fragments arranged by size: 2626 1766 1557 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3630 3876 669 Size: 988 281 1692 246 3079 Fragments arranged by size: 3079 1692 988 281 246 HindIII A'AGCT_T Cuts at: 748 3086 748 Size: 2338 3948 HpaI GTT'AAC Cuts at: 3876 3876 Size: 6286 KpnI G_GTAC'C Cuts at: 2458 3611 2458 Size: 1153 5133 MfeI C'AATT_G Cuts at: 2159 3885 2159 Size: 1726 4560 MluI A'CGCG_T Cuts at: 6162 6162 Size: 6286 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2205 5400 5584 1305 Size: 900 3195 184 2007 Fragments arranged by size: 3195 2007 900 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4464 Fragments arranged by size: 4464 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2865 4244 4603 4762 518 Size: 648 1699 1379 359 159 2042 Fragments arranged by size: 2042 1699 1379 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2774 3010 3507 3702 4379 5527 5772 1606 Size: 1168 236 497 195 677 1148 245 2120 Fragments arranged by size: 2120 1168 1148 677 497 245 236 195 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5734 NdeI CA'TA_TG Cuts at: 387 387 Size: 6286 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6286 NotI GC'GGCC_GC Cuts at: 3733 3733 Size: 6286 NsiI A_TGCA'T Cuts at: 3212 3212 Size: 6286 NspI r_CATG'y Cuts at: 1132 1229 3210 3644 5189 1132 Size: 97 1981 434 1545 2229 Fragments arranged by size: 2229 1981 1545 434 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6286 PciI A'CATG_T Cuts at: 5185 5185 Size: 6286 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6286 PmeI GTTT'AAAC Cuts at: 3590 3590 Size: 6286 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4355 4387 5868 5900 4355 Size: 32 1481 32 4741 Fragments arranged by size: 4741 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2503 1613 Size: 890 5396 PsiI TTA'TAA Cuts at: 2825 3856 2825 Size: 1031 5255 PstI C_TGCA'G Cuts at: 830 3638 830 Size: 2808 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6286 PvuII CAG'CTG Cuts at: 2774 3507 2774 Size: 733 5553 SalI G'TCGA_C Cuts at: 1936 3628 1936 Size: 1692 4594 SapI GCTCTTCn'nnn_ Cuts at: 2106 2106 Size: 6286 SbfI CC_TGCA'GG Cuts at: 3638 3638 Size: 6286 ScaI AGT'ACT Cuts at: 4534 6246 4534 Size: 1712 4574 SfcI C'TryA_G Cuts at: 826 1045 3634 4769 5450 5641 826 Size: 219 2589 1135 681 191 1471 Fragments arranged by size: 2589 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6286 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6286 SmaI CCC'GGG Cuts at: 1872 3613 1872 Size: 1741 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2311 4390 5291 5553 820 Size: 197 926 368 2079 901 262 1553 Fragments arranged by size: 2079 1553 926 901 368 262 197 SnaBI TAC'GTA Cuts at: 493 4077 493 Size: 3584 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6286 SphI G_CATG'C Cuts at: 1132 3644 1132 Size: 2512 3774 SspI AAT'ATT Cuts at: 5 52 4210 5 Size: 47 4158 2081 Fragments arranged by size: 4158 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6286 StyI C'CwwG_G Cuts at: 513 1065 3725 513 Size: 552 2660 3074 Fragments arranged by size: 3074 2660 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2147 4313 4330 4483 4668 5113 325 Size: 1255 567 2166 17 153 185 445 1498 Fragments arranged by size: 2166 1498 1255 567 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3330 3501 4532 Size: 274 80 33 51 1443 1353 171 1031 Cuts at: 4532 6244 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1353 1031 274 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6246 6278 6246 Size: 32 6254 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6286 XbaI T'CTAG_A Cuts at: 3622 3622 Size: 6286 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6286 XmaI C'CCGG_G Cuts at: 1870 3611 1870 Size: 1741 4545 XmnI GAAnn'nnTTC Cuts at: 2115 3315 4415 2115 Size: 1200 1100 3986 Fragments arranged by size: 3986 1200 1100 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4094 276 Size: 53 83 186 936 2560 2468 Fragments arranged by size: 2560 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI