Restriction map for
pFN21AE4104
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI ApaLI AscI AseI AsnI
Asp718I AspEI AvaII AvrII BamHI
BbrPI BbvCI BcgI BclI BfrI
BlnI BsaBI BsaI BsaWI BseAI
BsgI BsiWI BsmI BspDI BspEI
BspHI BspLU11I BspMI BsrDI BssSI
Bst1107I BstAPI BstBI BstXI BstZ17I
Bsu36I ClaI CpoI DrdI Eam1105I
EarI Eco81I EcoRI EcoRV EcoT22I
FbaI FseI HindIII HpaI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MunI NcoI NdeI NheI
NruI NsiI NspV PacI PaeR7I
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI SexAI SfcI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI XbaI
XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcsI 682 AAATTC [GA]^AATT[CT]
AflIII 794 ACACGT A^C[GA][CT]GT
AlwI 1167 GGATC GGATC(4/5)
Aor51HI 611 AGCGCT AGC^GCT
ApoI 682 AAATTC [GA]^AATT[CT]
Asp700I 1231 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 109 TGCGCA TGC^GCA
BbsI 739 GTCTTC GAAGAC(2/6)
BciVI 1199 GGATAC GTATCC(6/5)
BfaI 230 CTAG C^TAG
BglI 1126 GCCCAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 926 AGATCT A^GATCT
BlpI 186 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 186 GCTCAGC GC^T[ACGT]AGC
BpuAI 739 GTCTTC GAAGAC(2/6)
BsaAI 322 TACGTG [CT]AC^GT[GA]
BseRI 882 CTCCTC GAGGAG(10/8)
BsmAI 505 GTCTC GTCTC(1/5)
BsmBI 504 CGTCTC CGTCTC(1/5)
Bsp1407I 1191 TGTACA T^GTACA
BsrBI 382 GAGCGG CCGCTC(-3/-3)
BsrGI 1191 TGTACA T^GTACA
BstEII 447 GGTCACC G^GT[ACGT]ACC
BstPI 447 GGTCACC G^GT[ACGT]ACC
BstYI 926 AGATCT [GA]^GATC[CT]
CelII 186 GCTCAGC GC^T[ACGT]AGC
DraI 1339 TTTAAA TTT^AAA
DraIII 865 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 237 CCGTGG C^C[GA][CT]GG
Eco47III 611 AGCGCT AGC^GCT
EcoNI 846 CCTGTCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 447 GGTCACC G^GT[ACGT]ACC
FspI 109 TGCGCA TGC^GCA
HincII 904 GTCAAC GT[CT]^[GA]AC
HindII 904 GTCAAC GT[CT]^[GA]AC
HinfI 1269 GAATC G^A[ACGT]TC
MaeI 230 CTAG C^TAG
MflI 926 AGATCT [GA]^GATC[CT]
MspA1I 21 CAGCGG C[AC]G^C[GT]G
NaeI 856 GCCGGC GCC^GGC
NgoMIV 856 GCCGGC G^CCGGC
NotI 547 GCGGCCGC GC^GGCCGC
NspI 215 GCATGT [GA]CATG^[CT]
PflMI 864 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1060 GAGCTC GAGCT^C
ScaI 202 AGTACT AGT^ACT
SmlI 811 CTCAAG C^T[CT][GA]AG
SspBI 1191 TGTACA T^GTACA
StuI 833 AGGCCT AGG^CCT
TfiI 1269 GAATC G^A[AT]TC
Tsp509I 683 AATT ^AATT
Van91I 864 CCACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 44 CCATTAGCGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 926 AGATCT [GA]^GATC[CT]
XmnI 1231 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
ApaI 103 GGGCCC GGGCC^C
564 GGGCCC
AspHI 364 GAGCAC G[AT]GC[AT]^C
1060 GAGCTC
BalI 209 TGGCCA TGG^CCA
728 TGGCCA
BbeI 244 GGCGCC GGCGC^C
251 GGCGCC
BpmI 493 CTGGAG CTGGAG(16/14)
930 CTGGAG
BsiHKAI 364 GAGCAC G[AT]GC[AT]^C
1060 GAGCTC
BsrFI 776 ACCGGC [GA]^CCGG[CT]
856 GCCGGC
BstF5I 36 CATCC GGATG(2/0)
1118 CATCC
Cfr10I 776 ACCGGC [GA]^CCGG[CT]
856 GCCGGC
DraII 102 GGGGCCC [GA]G^G[ACGT]CC[CT]
103 GGGCCCT
EagI 86 CGGCCG C^GGCCG
548 CGGCCG
EclXI 86 CGGCCG C^GGCCG
548 CGGCCG
Eco52I 86 CGGCCG C^GGCCG
548 CGGCCG
EcoO109I 102 GGGGCCC [GA]G^G[ACGT]CC[CT]
103 GGGCCCT
EcoT14I 443 CCAAGG C^C[AT][AT]GG
731 CCAAGG
FokI 36 CATCC GGATG(9/13)
1118 CATCC
KasI 244 GGCGCC G^GCGCC
251 GGCGCC
MluNI 209 TGGCCA TGG^CCA
728 TGGCCA
MscI 209 TGGCCA TGG^CCA
728 TGGCCA
MseI 1159 TTAA T^TAA
1340 TTAA
NarI 244 GGCGCC GG^CGCC
251 GGCGCC
SfaNI 701 GCATC GCATC(5/9)
719 GCATC
SfoI 244 GGCGCC GGC^GCC
251 GGCGCC
StyI 443 CCAAGG C^C[AT][AT]GG
731 CCAAGG
TaqI 178 TCGA T^CGA
296 TCGA
Tru9I 1159 TTAA T^TAA
1340 TTAA
TthHB8I 178 TCGA T^CGA
296 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspI AvaI BanI BanII BbvI
BcnI BmyI BsaHI BsaJI BsiEI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssHII BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MslI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflFI RsaI
Sau3AI Sau96I ScrFI SmaI TseI
Tsp45I TspRI Tth111I XmaCI XmaI