Restriction map for
pFN21AE4104
(Circular) (Six-base) MAPSORT of: pFN21AE4104.seq Check: 716 from: 1 to: 6124
pFN21AE4104 6124 bp
With 182 enzymes: SgfI *
March 28, 2012 13:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3934 278
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3467 1937
Size: 1530 4594
Acc65I G'GTAC_C
Cuts at: 3445 3445
Size: 6124
AclI AA'CG_TT
Cuts at: 1563 4251 4624 1563
Size: 2688 373 3063
Fragments arranged by size:
3063 2688 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2033 2154 4187 5571 5839 1632
Size: 401 121 2033 1384 268 1917
Fragments arranged by size:
2033 1917 1384 401 268 121
AfeI AGC'GCT
Cuts at: 2618 2618
Size: 6124
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5927
AflIII A'CryG_T
Cuts at: 1203 2799 5023 6000 1203
Size: 1596 2224 977 1327
Fragments arranged by size:
2224 1596 1327 977
AgeI A'CCGG_T
Cuts at: 4955 4955
Size: 6124
AhdI GACnn_n'nnGTC
Cuts at: 4853 4853
Size: 6124
AleI CACnn'nnGTG
Cuts at: 1166 2586 2660 1166
Size: 1420 74 4630
Fragments arranged by size:
4630 1420 74
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2060 1184
Size: 876 5248
AlwNI CAG_nnn'CTG
Cuts at: 3867 5439 3867
Size: 1572 4552
ApaI G_GGCC'C
Cuts at: 2112 2573 2112
Size: 461 5663
ApaLI G'TGCA_C
Cuts at: 4181 5337 4181
Size: 1156 4968
ApoI r'AATT_y
Cuts at: 1493 2687 3433 3659 3811 5920 1493
Size: 1194 746 226 152 2109 1697
Fragments arranged by size:
2109 1697 1194 746 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4678 160
Size: 872 3646 1606
Fragments arranged by size:
3646 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
AvaI C'yCGr_G
Cuts at: 1870 1943 2104 2378 2566 2572 2620 2890 3264
Size: 73 161 274 188 6 48 270 374
Cuts at: 3264 3449 1870
Size: 185 4545
Fragments arranged by size:
4545 374 274 270 188 185 161 73
48 6
BamHI G'GATC_C
Cuts at: 3454 3821 3454
Size: 367 5757
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 2256 2894 3445 4900
Size: 325 217 627 462 7 638 551 1455
Cuts at: 4900 618
Size: 1842
Fragments arranged by size:
1842 1455 638 627 551 462 325 217
7
BanII G_rGCy'C
Cuts at: 1391 2112 2573 2651 3069 3443 1391
Size: 721 461 78 418 374 4072
Fragments arranged by size:
4072 721 461 418 374 78
BbeI G_GCGC'C
Cuts at: 2253 2260 2253
Size: 7 6117
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2737 928
Size: 971 838 4315
Fragments arranged by size:
4315 971 838
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4315 4349 1903
Size: 34 2378 34 3678
Fragments arranged by size:
3678 2378 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3198 4016 5226 3198
Size: 818 1210 4096
Fragments arranged by size:
4096 1210 818
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6124
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3137 4735 136
Size: 107 122 71 631 605 1465 1598 1525
Fragments arranged by size:
1598 1525 1465 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2931 6119 2931
Size: 3188 2936
BlpI GC'TnA_GC
Cuts at: 2192 3541 2192
Size: 1349 4775
Bme1580I G_kGCm'C
Cuts at: 1790 2112 2565 2573 2897 3133 4185 5341 1790
Size: 322 453 8 324 236 1052 1156 2573
Fragments arranged by size:
2573 1156 1052 453 324 322 236 8
BmgBI CAC'GTC
Cuts at: 1318 2802 1318
Size: 1484 4640
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3855 4813 442
Size: 416 53 83 399 24 2438 958 1753
Fragments arranged by size:
2438 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2519 2956 4784 1239
Size: 1280 437 1828 2579
Fragments arranged by size:
2579 1828 1280 437
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2801 4249 5114 5412 2801
Size: 1448 865 298 3513
Fragments arranged by size:
3513 1448 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4787 882
Size: 617 3288 2219
Fragments arranged by size:
3288 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2329 3915 493
Size: 711 1125 1586 2702
Fragments arranged by size:
2702 1586 1125 711
BsaBI GATnn'nnATC
Cuts at: 6118 6118
Size: 6124
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2250 2257 2682 3931
Size: 53 83 186 936 717 7 425 1249
Cuts at: 3931 4313 4948 275
Size: 382 635 1451
Fragments arranged by size:
1451 1249 936 717 635 425 382 186
83 53 7
BsaWI w'CCGG_w
Cuts at: 4557 4955 5229 5376 5787 4557
Size: 398 274 147 411 4894
Fragments arranged by size:
4894 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2633 2663 1178
Size: 30 165 30 1230 30 4639
Fragments arranged by size:
4639 1230 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2878 1186
Size: 1692 4432
BseYI C'CCAG_C
Cuts at: 2635 3132 5327 5823 2635
Size: 497 2195 496 2936
Fragments arranged by size:
2936 2195 497 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5551
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2094 2305 2556 2782 3574 4335
Size: 275 186 93 211 251 226 792 761
Cuts at: 4335 5363 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 792 761 275 251 226 211
186 93
BsiHKAI G_wGCw'C
Cuts at: 2373 3069 4185 4270 5341 2373
Size: 696 1116 85 1071 3156
Fragments arranged by size:
3156 1116 1071 696 85
BsmI GAATG_Cn'
Cuts at: 3635 3728 3635
Size: 93 6031
BsmBI CGTCTCn'nnnn_
Cuts at: 2515 2515
Size: 6124
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2373 2565 2573 2651 2897 3069
Size: 399 322 261 192 8 78 246 172
Cuts at: 3069 3133 3443 4185 4270 5341 1391
Size: 64 310 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 322 310 261 246
192 172 85 78 64 8
BspEI T'CCGG_A
Cuts at: 5787 5787
Size: 6124
BspHI T'CATG_A
Cuts at: 4013 5743 4013
Size: 1730 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3479 844
Size: 2635 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2389 4011 1394
Size: 429 566 1622 3507
Fragments arranged by size:
3507 1622 566 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4619 4793 65
Size: 1183 3371 174 1396
Fragments arranged by size:
3371 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2781 2861 4768 4955 1700
Size: 110 971 80 1907 187 2869
Fragments arranged by size:
2869 1907 971 187 110 80
BsrGI T'GTAC_A
Cuts at: 96 3196 96
Size: 3100 3024
BssHII G'CGCG_C
Cuts at: 1925 2288 2604 2702 1925
Size: 363 316 98 5347
Fragments arranged by size:
5347 363 316 98
BssSI C'ACGA_G
Cuts at: 4184 5196 4184
Size: 1012 5112
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6124
BstBI TT'CG_AA
Cuts at: 4928 4928
Size: 6124
BstEII G'GTnAC_C
Cuts at: 1184 2452 1184
Size: 1268 4856
BstYI r'GATC_y
Cuts at: 1972 2931 3454 3821 4206 4223 5675 5761 5773
Size: 959 523 367 385 17 1452 86 12
Cuts at: 5773 6119 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 959 523 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2242 513
Size: 552 939 238 4395
Fragments arranged by size:
4395 939 552 238
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2710 487
Size: 683 56 1484 3901
Fragments arranged by size:
3901 1484 683 56
BtsI GCAGTG_nn'
Cuts at: 3640 4434 4454 3640
Size: 794 20 5310
Fragments arranged by size:
5310 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6089
DraI TTT'AAA
Cuts at: 3346 3428 3346
Size: 82 6042
DraIII CAC_nnn'GTG
Cuts at: 2875 2875
Size: 6124
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5131 809
Size: 507 3815 1802
Fragments arranged by size:
3815 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2158 2214
Size: 54 1425 50 275 18 261 67 56
Cuts at: 2214 2553 2733 3571 4460 6089 8
Size: 339 180 838 889 1629 43
Fragments arranged by size:
1629 1425 889 838 339 275 261 180
67 56 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2091 2553 3571 1537
Size: 275 279 462 1018 4090
Fragments arranged by size:
4090 1018 462 279 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4054 1353
Size: 2701 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4711 5085 5231 387
Size: 1064 138 3122 374 146 1280
Fragments arranged by size:
3122 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3067 3067
Size: 6124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2033 2154 2519 2956 4187 4784 5571
Size: 393 401 121 365 437 1231 597 787
Cuts at: 5571 5839 1239
Size: 268 1524
Fragments arranged by size:
1524 1231 787 597 437 401 393 365
268 121
EcoNI CCTnn'n_nnAGG
Cuts at: 2855 2855
Size: 6124
EcoO109I rG'GnC_Cy
Cuts at: 2108 2109 2108
Size: 1 6123
EcoRI G'AATT_C
Cuts at: 3433 3811 3433
Size: 378 5746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2761 3069 2761
Size: 308 5816
FspI TGC'GCA
Cuts at: 2116 4630 2116
Size: 2514 3610
HaeII r_GCGC'y
Cuts at: 1678 2253 2260 2620 5271 1678
Size: 575 7 360 2651 2531
Fragments arranged by size:
2651 2531 575 360 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2495 2527 2603 2635 2837
Size: 32 135 32 923 32 76 32 202
Cuts at: 2837 2869 3107 3139 3164 3196 4765 4797 4839
Size: 32 238 32 25 32 1569 32 42
Cuts at: 4839 4871 1373
Size: 32 2626
Fragments arranged by size:
2626 1569 923 238 202 135 76 42
32 32 32 32 32 32 32 32
32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 2911 3468 3714 669
Size: 988 281 973 557 246 3079
Fragments arranged by size:
3079 988 973 557 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6124
HpaI GTT'AAC
Cuts at: 3714 3714
Size: 6124
KasI G'GCGC_C
Cuts at: 2249 2256 2249
Size: 7 6117
KpnI G_GTAC'C
Cuts at: 3449 3449
Size: 6124
MfeI C'AATT_G
Cuts at: 3723 3723
Size: 6124
MluI A'CGCG_T
Cuts at: 6000 6000
Size: 6124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2989 5238 5422 1305
Size: 1684 2249 184 2007
Fragments arranged by size:
2249 2007 1684 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2216 2735 10
Size: 54 1425 343 384 519 3399
Fragments arranged by size:
3399 1425 519 384 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2586 2660 2711 4082 4441 4600 518
Size: 648 1420 74 51 1371 359 159 2042
Fragments arranged by size:
2042 1420 1371 648 359 159 74 51
MspA1I CmG'CkG
Cuts at: 1606 2028 3540 4217 5365 5610 1606
Size: 422 1512 677 1148 245 2120
Fragments arranged by size:
2120 1512 1148 677 422 245
NaeI GCC'GGC
Cuts at: 2863 2863
Size: 6124
NarI GG'CG_CC
Cuts at: 2250 2257 2250
Size: 7 6117
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4633
Fragments arranged by size:
4633 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6124
NgoMIV G'CCGG_C
Cuts at: 2861 2861
Size: 6124
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6124
NotI GC'GGCC_GC
Cuts at: 2553 3571 2553
Size: 1018 5106
NspI r_CATG'y
Cuts at: 1132 1229 2224 3482 5027 1132
Size: 97 995 1258 1545 2229
Fragments arranged by size:
2229 1545 1258 995 97
PasI CC'CwG_GG
Cuts at: 1348 2274 1348
Size: 926 5198
PciI A'CATG_T
Cuts at: 5023 5023
Size: 6124
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2875 1108
Size: 1767 4357
PfoI T'CCnGG_A
Cuts at: 2199 2199
Size: 6124
PmeI GTTT'AAAC
Cuts at: 3428 3428
Size: 6124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4193 4225 5706 5738 4193
Size: 32 1481 32 4579
Fragments arranged by size:
4579 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6124
PsiI TTA'TAA
Cuts at: 3694 3694
Size: 6124
PspOMI G'GGCC_C
Cuts at: 2108 2569 2108
Size: 461 5663
PstI C_TGCA'G
Cuts at: 830 3476 830
Size: 2646 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6124
SacI G_AGCT'C
Cuts at: 3069 3069
Size: 6124
SalI G'TCGA_C
Cuts at: 1936 3466 1936
Size: 1530 4594
SbfI CC_TGCA'GG
Cuts at: 3476 3476
Size: 6124
ScaI AGT'ACT
Cuts at: 2209 4372 6084 2209
Size: 2163 1712 2249
Fragments arranged by size:
2249 2163 1712
SfcI C'TryA_G
Cuts at: 826 1045 3472 4607 5288 5479 826
Size: 219 2427 1135 681 191 1471
Fragments arranged by size:
2427 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2251 2258 2251
Size: 7 6117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6124
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6124
SmaI CCC'GGG
Cuts at: 1872 2106 2574 3266 3451 1872
Size: 234 468 692 185 4545
Fragments arranged by size:
4545 692 468 234 185
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2816 4228 5129 5391 820
Size: 197 926 873 1412 901 262 1553
Fragments arranged by size:
1553 1412 926 901 873 262 197
SnaBI TAC'GTA
Cuts at: 493 3915 493
Size: 3422 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6124
SphI G_CATG'C
Cuts at: 1132 3482 1132
Size: 2350 3774
SspI AAT'ATT
Cuts at: 5 52 4048 5
Size: 47 3996 2081
Fragments arranged by size:
3996 2081 47
StuI AGG'CCT
Cuts at: 1517 2840 1517
Size: 1323 4801
StyI C'CwwG_G
Cuts at: 513 1065 2004 2448 2736 3563 513
Size: 552 939 444 288 827 3074
Fragments arranged by size:
3074 939 827 552 444 288
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4151 4168 4321 4506 4951 325
Size: 1255 2571 17 153 185 445 1498
Fragments arranged by size:
2571 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2207 2796 2952
Size: 274 80 33 51 1443 230 589 156
Cuts at: 2952 3196 4370 6082 96
Size: 244 1174 1712 138
Fragments arranged by size:
1712 1443 1174 589 274 244 230 156
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6084 6116 6084
Size: 32 6092
Tth111I GACn'n_nGTC
Cuts at: 1316 2429 2507 3023 1316
Size: 1113 78 516 4417
Fragments arranged by size:
4417 1113 516 78
XbaI T'CTAG_A
Cuts at: 3460 3460
Size: 6124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2056 2056
Size: 6124
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6124
XmaI C'CCGG_G
Cuts at: 1870 2104 2572 3264 3449 1870
Size: 234 468 692 185 4545
Fragments arranged by size:
4545 692 468 234 185
XmnI GAAnn'nnTTC
Cuts at: 3240 4253 3240
Size: 1013 5111
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3932 276
Size: 53 83 186 936 2398 2468
Fragments arranged by size:
2468 2398 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI
NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII
RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI