Restriction map for
pFN21AE4104
(Linear) (Six-base) MAPSORT of: KIEE4104.seq Check: 7987 from: 1 to: 1419
KIEE4104.orf
With 182 enzymes: SgfI *
March 22, 2012 17:22 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 149 1419
Size: 28 121 1270
Fragments arranged by size:
1270 121 28
AfeI AGC'GCT
Cuts at: 0 613 1419
Size: 613 806
AflIII A'CryG_T
Cuts at: 0 794 1419
Size: 794 625
AleI CACnn'nnGTG
Cuts at: 0 581 655 1419
Size: 581 74 764
Fragments arranged by size:
764 581 74
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 55 1419
Size: 55 1364
ApaI G_GGCC'C
Cuts at: 0 107 568 1419
Size: 107 461 851
Fragments arranged by size:
851 461 107
ApoI r'AATT_y
Cuts at: 0 682 1419
Size: 682 737
AvaI C'yCGr_G
Cuts at: 0 99 373 561 567 615 885 1259 1419
Size: 99 274 188 6 48 270 374 160
Fragments arranged by size:
374 274 270 188 160 99 48 6
BanI G'GyrC_C
Cuts at: 0 244 251 889 1419
Size: 244 7 638 530
Fragments arranged by size:
638 530 244 7
BanII G_rGCy'C
Cuts at: 0 107 568 646 1064 1419
Size: 107 461 78 418 355
Fragments arranged by size:
461 418 355 107 78
BbeI G_GCGC'C
Cuts at: 0 248 255 1419
Size: 248 7 1164
Fragments arranged by size:
1164 248 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 732 1419
Size: 732 687
BciVI GTATCCnnnnn_n'
Cuts at: 0 1193 1419
Size: 1193 226
BglI GCCn_nnn'nGGC
Cuts at: 0 1132 1419
Size: 1132 287
BglII A'GATC_T
Cuts at: 0 926 1419
Size: 926 493
BlpI GC'TnA_GC
Cuts at: 0 187 1419
Size: 187 1232
Bme1580I G_kGCm'C
Cuts at: 0 107 560 568 892 1128 1419
Size: 107 453 8 324 236 291
Fragments arranged by size:
453 324 291 236 107 8
BmgBI CAC'GTC
Cuts at: 0 797 1419
Size: 797 622
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 951 1419
Size: 514 437 468
Fragments arranged by size:
514 468 437
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 1419
Size: 796 623
BsaAI yAC'GTr
Cuts at: 0 324 1419
Size: 324 1095
BsaHI Gr'CG_yC
Cuts at: 0 245 252 677 1419
Size: 245 7 425 742
Fragments arranged by size:
742 425 245 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 628 658 1419
Size: 628 30 761
Fragments arranged by size:
761 628 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 873 1419
Size: 873 546
BseYI C'CCAG_C
Cuts at: 0 630 1127 1419
Size: 630 497 292
Fragments arranged by size:
630 497 292
BsiEI CG_ry'CG
Cuts at: 0 89 300 551 777 1419
Size: 89 211 251 226 642
Fragments arranged by size:
642 251 226 211 89
BsiHKAI G_wGCw'C
Cuts at: 0 368 1064 1419
Size: 368 696 355
Fragments arranged by size:
696 368 355
BsmBI CGTCTCn'nnnn_
Cuts at: 0 510 1419
Size: 510 909
Bsp1286I G_dGCh'C
Cuts at: 0 107 368 560 568 646 892 1064 1128
Size: 107 261 192 8 78 246 172 64
Cuts at: 1128 1419
Size: 291
Fragments arranged by size:
291 261 246 192 172 107 78 64
8
BsrBI CCG'CTC
Cuts at: 0 384 1419
Size: 384 1035
BsrFI r'CCGG_y
Cuts at: 0 776 856 1419
Size: 776 80 563
Fragments arranged by size:
776 563 80
BsrGI T'GTAC_A
Cuts at: 0 1191 1419
Size: 1191 228
BssHII G'CGCG_C
Cuts at: 0 283 599 697 1419
Size: 283 316 98 722
Fragments arranged by size:
722 316 283 98
BstEII G'GTnAC_C
Cuts at: 0 447 1419
Size: 447 972
BstYI r'GATC_y
Cuts at: 0 926 1419
Size: 926 493
BtgI C'CryG_G
Cuts at: 0 237 1419
Size: 237 1182
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 705 1419
Size: 705 714
DraI TTT'AAA
Cuts at: 0 1341 1419
Size: 1341 78
DraIII CAC_nnn'GTG
Cuts at: 0 870 1419
Size: 870 549
EaeI y'GGCC_r
Cuts at: 0 86 153 209 548 728 1419
Size: 86 67 56 339 180 691
Fragments arranged by size:
691 339 180 86 67 56
EagI C'GGCC_G
Cuts at: 0 86 548 1419
Size: 86 462 871
Fragments arranged by size:
871 462 86
EcoICRI GAG'CTC
Cuts at: 0 1062 1419
Size: 1062 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 149 514 951 1419
Size: 28 121 365 437 468
Fragments arranged by size:
468 437 365 121 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 850 1419
Size: 850 569
EcoO109I rG'GnC_Cy
Cuts at: 0 103 104 1419
Size: 103 1 1315
Fragments arranged by size:
1315 103 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 756 1064 1419
Size: 756 308 355
Fragments arranged by size:
756 355 308
FspI TGC'GCA
Cuts at: 0 111 1419
Size: 111 1308
HaeII r_GCGC'y
Cuts at: 0 248 255 615 1419
Size: 248 7 360 804
Fragments arranged by size:
804 360 248 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 490 522 598 630 832 864 1102 1134
Size: 490 32 76 32 202 32 238 32
Cuts at: 1134 1159 1191 1419
Size: 25 32 228
Fragments arranged by size:
490 238 228 202 76 32 32 32
32 32 25
HincII GTy'rAC
Cuts at: 0 906 1419
Size: 906 513
KasI G'GCGC_C
Cuts at: 0 244 251 1419
Size: 244 7 1168
Fragments arranged by size:
1168 244 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 984 1419
Size: 984 435
MscI TGG'CCA
Cuts at: 0 211 730 1419
Size: 211 519 689
Fragments arranged by size:
689 519 211
MslI CAynn'nnrTG
Cuts at: 0 581 655 706 1419
Size: 581 74 51 713
Fragments arranged by size:
713 581 74 51
MspA1I CmG'CkG
Cuts at: 0 23 1419
Size: 23 1396
NaeI GCC'GGC
Cuts at: 0 858 1419
Size: 858 561
NarI GG'CG_CC
Cuts at: 0 245 252 1419
Size: 245 7 1167
Fragments arranged by size:
1167 245 7
NgoMIV G'CCGG_C
Cuts at: 0 856 1419
Size: 856 563
NotI GC'GGCC_GC
Cuts at: 0 548 1419
Size: 548 871
NspI r_CATG'y
Cuts at: 0 219 1419
Size: 219 1200
PasI CC'CwG_GG
Cuts at: 0 269 1419
Size: 269 1150
PflMI CCAn_nnn'nTGG
Cuts at: 0 870 1419
Size: 870 549
PfoI T'CCnGG_A
Cuts at: 0 194 1419
Size: 194 1225
PspOMI G'GGCC_C
Cuts at: 0 103 564 1419
Size: 103 461 855
Fragments arranged by size:
855 461 103
SacI G_AGCT'C
Cuts at: 0 1064 1419
Size: 1064 355
ScaI AGT'ACT
Cuts at: 0 204 1419
Size: 204 1215
SfoI GGC'GCC
Cuts at: 0 246 253 1419
Size: 246 7 1166
Fragments arranged by size:
1166 246 7
SmaI CCC'GGG
Cuts at: 0 101 569 1261 1419
Size: 101 468 692 158
Fragments arranged by size:
692 468 158 101
SmlI C'TyrA_G
Cuts at: 0 811 1419
Size: 811 608
StuI AGG'CCT
Cuts at: 0 835 1419
Size: 835 584
StyI C'CwwG_G
Cuts at: 0 443 731 1419
Size: 443 288 688
Fragments arranged by size:
688 443 288
TatI w'GTAC_w
Cuts at: 0 202 791 947 1191 1419
Size: 202 589 156 244 228
Fragments arranged by size:
589 244 228 202 156
Tth111I GACn'n_nGTC
Cuts at: 0 424 502 1018 1419
Size: 424 78 516 401
Fragments arranged by size:
516 424 401 78
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 51 1419
Size: 51 1368
XmaI C'CCGG_G
Cuts at: 0 99 567 1259 1419
Size: 99 468 692 160
Fragments arranged by size:
692 468 160 99
XmnI GAAnn'nnTTC
Cuts at: 0 1235 1419
Size: 1235 184
Enzymes that do cut:
AcuI AfeI AflIII AleI AlfI ApaI ApoI AvaI
BanI BanII BbeI BbsI BciVI BglI BglII BlpI
Bme1580I BmgBI BpmI BpuEI BsaAI BsaHI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrFI BsrGI
BssHII BstEII BstYI BtgI BtgZI DraI DraIII EaeI
EagI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NotI NspI PasI PflMI PfoI PspOMI
SacI ScaI SfoI SmaI SmlI StuI StyI TatI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BcgI BclI BfrBI BmrI BmtI BplI
Bpu10I BsaI BsaBI BsaWI BsgI BsiWI BsmI BspEI
BspHI BspMI BsrDI BssSI BstAPI BstBI BstXI BstZ17I
Bsu36I BtsI ClaI CspCI DrdI EarI EciI EcoRI
EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI
NcoI NdeI NheI NruI NsiI PacI PciI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TstI XbaI XhoI ZraI