Restriction map for
pFN21AE5731
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# NO CUT ENZYMES ( 86 enzymes) #
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Acc65I AccIII AflII AgeI AhdI
Aor51HI AseI AsnI Asp718I AspEI
AspI BbeI BbrPI BcgI BfrI
BglII BlpI Bpu1102I BsaBI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BsrGI BstAPI BstBI CelII
Cfr10I ClaI CpoI DraI Eam1105I
Eco47III EcoNI EcoT22I FseI HindIII
HpaI KasI KpnI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
RsrII SbfI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AatII 1762 GACGTC GACGT^C
AclI 1579 AACGTT AA^CGTT
Alw44I 856 GTGCAC G^TGCAC
AlwNI 1499 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1720 CCTCAGG CC^T[ACGT]AGG
ApaLI 856 GTGCAC G^TGCAC
AscI 82 GGCGCGCC GG^CGCGCC
Asp700I 789 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 749 TGCGCA TGC^GCA
AvrII 1177 CCTAGG C^CTAGG
BalI 944 TGGCCA TGG^CCA
BamHI 470 GGATCC G^GATCC
BbsI 217 GTCTTC GAAGAC(2/6)
BbvCI 1009 GCTGAGG CCTCAGC(-5/-2)
BciVI 1702 GGATAC GTATCC(6/5)
BclI 1427 TGATCA T^GATCA
BfaI 1178 CTAG C^TAG
BlnI 1177 CCTAGG C^CTAGG
BpuAI 217 GTCTTC GAAGAC(2/6)
BseRI 505 GAGGAG GAGGAG(10/8)
BsrDI 1437 CATTGC GCAATG(2/0)
Bst1107I 1561 GTATAC GTA^TAC
BstEII 519 GGTCACC G^GT[ACGT]ACC
BstPI 519 GGTCACC G^GT[ACGT]ACC
BstXI 947 CCAAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1561 GTATAC GTA^TAC
Bsu36I 1720 CCTCAGG CC^T[ACGT]AGG
DraIII 1411 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 538 GACGTGATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1720 CCTCAGG CC^T[ACGT]AGG
EcoO65I 519 GGTCACC G^GT[ACGT]ACC
EcoRI 1589 GAATTC G^AATTC
EcoRV 1229 GATATC GAT^ATC
FbaI 1427 TGATCA T^GATCA
FspI 749 TGCGCA TGC^GCA
HaeII 108 GGCGCT [GA]GCGC^[CT]
MaeI 1178 CTAG C^TAG
MluNI 944 TGGCCA TGG^CCA
MscI 944 TGGCCA TGG^CCA
MseI 1316 TTAA T^TAA
MslI 1437 CATTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 623 CCATGG C^CATGG
NspI 885 GCATGC [GA]CATG^[CT]
PflMI 1410 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 37 GACTC GAGTC(4/5)
Psp1406I 1579 AACGTT AA^CGTT
PstI 435 CTGCAG CTGCA^G
PvuI 1652 CGATCG CGAT^CG
SacI 388 GAGCTC GAGCT^C
SalI 1521 GTCGAC G^TCGAC
SapI 789 GAAGAGC GCTCTTC(1/4)
ScaI 968 AGTACT AGT^ACT
SexAI 1320 ACCTGGT A^CC[AT]GGT
SmaI 411 CCCGGG CCC^GGG
SphI 885 GCATGC GCATG^C
StuI 1724 AGGCCT AGG^CCT
TfiI 13 GATTC G^A[AT]TC
Tru9I 1316 TTAA T^TAA
Van91I 1410 CCACACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 172 CCACCGCCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 411 CCCGGG C^CCGGG
XmaI 411 CCCGGG C^CCGGG
XmnI 789 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcsI 1281 GAATTT [GA]^AATT[CT]
1589 GAATTC
AcyI 1524 GACGCC G[GA]^CG[CT]C
1762 GACGTC
AflIII 242 ACGTGT A^C[GA][CT]GT
1034 ACGTGT
ApaI 189 GGGCCC GGGCC^C
1096 GGGCCC
ApoI 1281 GAATTT [GA]^AATT[CT]
1589 GAATTC
AvaII 50 GGTCC G^G[AT]CC
1749 GGTCC
BsaHI 1524 GACGCC G[GA]^CG[CT]C
1762 GACGTC
BsaI 727 GAGACC GGTCTC(1/5)
766 GAGACC
BsgI 1151 GTGCAG GTGCAG(16/14)
1444 GTGCAG
BsmAI 727 GAGAC GTCTC(1/5)
766 GAGAC
BsmFI 1106 GGGAC GGGAC(10/14)
1750 GTCCC
BssHII 83 GCGCGC G^CGCGC
375 GCGCGC
BssSI 364 CACGAG CACGAG(-5/-1)
924 CTCGTG
EarI 457 CTCTTC CTCTTC(1/4)
789 GAAGAG
HgaI 1524 GACGC GACGC(5/10)
827 GCGTC
Hin1I 1524 GACGCC G[GA]^CG[CT]C
1762 GACGTC
HincII 1300 GTCAAC GT[CT]^[GA]AC
1521 GTCGAC
HindII 1300 GTCAAC GT[CT]^[GA]AC
1521 GTCGAC
HinfI 13 GATTC G^A[ACGT]TC
37 GACTC
HphI 521 TCACC GGTGA(8/7)
863 TCACC
Ksp632I 457 CTCTTC CTCTTC(1/4)
789 GAAGAG
NotI 1483 GCGGCCGC GC^GGCCGC
1684 GCGGCCGC
PpuMI 49 GGGTCCT [GA]G^G[AT]CC[CT]
1748 AGGTCCC
PvuII 658 CAGCTG CAG^CTG
1154 CAGCTG
Tsp509I 1282 AATT ^AATT
1590 AATT
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccI AccII AciI AfaI AluI
AlwI AspHI AvaI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaAI BsaJI BsiEI BsiHKAI BsiYI
BslI BsoBI Bsp1286I BspHI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII ItaI KspI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV RcaI
RsaI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp45I TspRI TthHB8I XhoII