(Circular) (Six-base) MAPSORT of: pFN21AE5731.seq Check: 3436 from: 1 to: 6484 pFN21AE5731 6484 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3771 4294 278 Size: 53 83 186 936 2235 523 2468 Fragments arranged by size: 2468 2235 936 523 186 83 53 AccI GT'mk_AC Cuts at: 1937 2553 3527 3567 3771 3827 1937 Size: 616 974 40 204 56 4594 Fragments arranged by size: 4594 974 616 204 56 40 Acc65I G'GTAC_C Cuts at: 3805 3805 Size: 6484 AclI AA'CG_TT Cuts at: 1563 3585 4611 4984 1563 Size: 2022 1026 373 3063 Fragments arranged by size: 3063 2022 1026 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4547 5931 6199 1632 Size: 2915 1384 268 1917 Fragments arranged by size: 2915 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6287 AflIII A'CryG_T Cuts at: 1203 2247 3039 5383 6360 1203 Size: 1044 792 2344 977 1327 Fragments arranged by size: 2344 1327 1044 977 792 AgeI A'CCGG_T Cuts at: 5315 5315 Size: 6484 AhdI GACnn_n'nnGTC Cuts at: 5213 5213 Size: 6484 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6484 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2663 1184 Size: 1479 5005 AlwNI CAG_nnn'CTG Cuts at: 3509 4227 5799 3509 Size: 718 1572 4194 Fragments arranged by size: 4194 1572 718 ApaI G_GGCC'C Cuts at: 2198 3105 2198 Size: 907 5577 ApaLI G'TGCA_C Cuts at: 2861 4541 5697 2861 Size: 1680 1156 3648 Fragments arranged by size: 3648 1680 1156 ApoI r'AATT_y Cuts at: 1493 3286 3594 3793 4019 4171 6280 1493 Size: 1793 308 199 226 152 2109 1697 Fragments arranged by size: 2109 1793 1697 308 226 199 152 AscI GG'CGCG_CC Cuts at: 2088 2088 Size: 6484 AseI AT'TA_AT Cuts at: 160 1032 5038 160 Size: 872 4006 1606 Fragments arranged by size: 4006 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 AvaI C'yCGr_G Cuts at: 1870 1943 2072 2416 3197 3809 1870 Size: 73 129 344 781 612 4545 Fragments arranged by size: 4545 781 612 344 129 73 AvrII C'CTAG_G Cuts at: 3182 3182 Size: 6484 BamHI G'GATC_C Cuts at: 2475 3814 4181 2475 Size: 1339 367 4778 Fragments arranged by size: 4778 1339 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2068 2266 3280 3805 5260 Size: 325 217 627 281 198 1014 525 1455 Cuts at: 5260 618 Size: 1842 Fragments arranged by size: 1842 1455 1014 627 525 325 281 217 198 BanII G_rGCy'C Cuts at: 1391 2198 2397 2930 3105 3175 3358 3803 1391 Size: 807 199 533 175 70 183 445 4072 Fragments arranged by size: 4072 807 533 445 199 183 175 70 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2215 928 Size: 971 316 5197 Fragments arranged by size: 5197 971 316 BbvCI CC'TCA_GC Cuts at: 3015 3015 Size: 6484 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4675 4709 1903 Size: 34 2738 34 3678 Fragments arranged by size: 3678 2738 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3701 4376 5586 3701 Size: 675 1210 4599 Fragments arranged by size: 4599 1210 675 BclI T'GATC_A Cuts at: 1548 3432 1548 Size: 1884 4600 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2430 2564 3353 Size: 107 122 71 631 605 758 134 789 Cuts at: 3353 5095 136 Size: 1742 1525 Fragments arranged by size: 1742 1525 789 758 631 605 134 122 107 71 BglII A'GATC_T Cuts at: 6479 6479 Size: 6484 BlpI GC'TnA_GC Cuts at: 3901 3901 Size: 6484 Bme1580I G_kGCm'C Cuts at: 1790 2073 2198 2271 2865 3105 4545 5701 1790 Size: 283 125 73 594 240 1440 1156 2573 Fragments arranged by size: 2573 1440 1156 594 283 240 125 73 BmgBI CAC'GTC Cuts at: 1318 2248 2545 2713 2725 1318 Size: 930 297 168 12 5077 Fragments arranged by size: 5077 930 297 168 12 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2701 3305 4215 Size: 416 53 83 399 24 1284 604 910 Cuts at: 4215 5173 442 Size: 958 1753 Fragments arranged by size: 1753 1284 958 910 604 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6484 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2992 2992 Size: 6484 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2528 2942 2957 3366 3546 3627 5144 1239 Size: 1289 414 15 409 180 81 1517 2579 Fragments arranged by size: 2579 1517 1289 414 409 180 81 15 Bpu10I CC'TnA_GC Cuts at: 3015 3015 Size: 6484 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4609 5474 5772 4609 Size: 865 298 5321 Fragments arranged by size: 5321 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2726 2765 5147 882 Size: 617 1227 39 2382 2219 Fragments arranged by size: 2382 2219 1227 617 39 BsaAI yAC'GTr Cuts at: 493 1204 2752 3040 3628 4275 493 Size: 711 1548 288 588 647 2702 Fragments arranged by size: 2702 1548 711 647 588 288 BsaBI GATnn'nnATC Cuts at: 6478 6478 Size: 6484 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3530 3768 4291 4673 Size: 53 83 186 936 1997 238 523 382 Cuts at: 4673 5308 275 Size: 635 1451 Fragments arranged by size: 1997 1451 936 635 523 382 238 186 83 53 BsaWI w'CCGG_w Cuts at: 4917 5315 5589 5736 6147 4917 Size: 398 274 147 411 5254 Fragments arranged by size: 5254 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2915 2945 3029 3059 3562 Size: 30 165 30 1512 30 84 30 503 Cuts at: 3562 3592 1178 Size: 30 4070 Fragments arranged by size: 4070 1512 503 165 84 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2525 1186 Size: 1339 5145 BseYI C'CCAG_C Cuts at: 2187 3032 3460 5687 6183 2187 Size: 845 428 2227 496 2488 Fragments arranged by size: 2488 2227 845 496 428 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3177 3470 1163 Size: 573 1441 293 4177 Fragments arranged by size: 4177 1441 573 293 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 2568 3492 3660 3693 3934 Size: 275 186 48 519 924 168 33 241 Cuts at: 3934 4695 5723 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 924 761 519 275 241 186 168 48 33 BsiHKAI G_wGCw'C Cuts at: 2397 2865 3429 4545 4630 5701 2397 Size: 468 564 1116 85 1071 3180 Fragments arranged by size: 3180 1116 1071 564 468 85 BsmI GAATG_Cn' Cuts at: 3995 4088 3995 Size: 93 6391 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2198 2271 2397 2865 2930 3105 Size: 399 283 125 73 126 468 65 175 Cuts at: 3105 3175 3358 3429 3803 4545 4630 5701 1391 Size: 70 183 71 374 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 468 399 374 283 183 175 126 125 85 73 71 70 65 BspEI T'CCGG_A Cuts at: 6147 6147 Size: 6484 BspHI T'CATG_A Cuts at: 2310 2763 3238 4373 6103 2310 Size: 453 475 1135 1730 2691 Fragments arranged by size: 2691 1730 1135 475 453 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3839 844 Size: 2995 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4371 1394 Size: 429 2548 3507 Fragments arranged by size: 3507 2548 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3441 4979 5153 65 Size: 1183 2193 1538 174 1396 Fragments arranged by size: 2193 1538 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5128 5315 1700 Size: 110 3318 187 2869 Fragments arranged by size: 3318 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6484 BssHII G'CGCG_C Cuts at: 1925 2088 2380 1925 Size: 163 292 6029 Fragments arranged by size: 6029 292 163 BssSI C'ACGA_G Cuts at: 2369 2929 4544 5556 2369 Size: 560 1615 1012 3297 Fragments arranged by size: 3297 1615 1012 560 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6484 BstBI TT'CG_AA Cuts at: 5288 5288 Size: 6484 BstEII G'GTnAC_C Cuts at: 1184 2524 1184 Size: 1340 5144 BstXI CCAn_nnnn'nTGG Cuts at: 2959 2959 Size: 6484 BstYI r'GATC_y Cuts at: 1972 2235 2409 2475 2742 2841 3609 3814 4181 Size: 263 174 66 267 99 768 205 367 Cuts at: 4181 4566 4583 6035 6121 6133 6479 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 768 385 367 346 267 263 205 174 99 86 66 17 12 BstZ17I GTA'TAC Cuts at: 3568 3568 Size: 6484 Bsu36I CC'TnA_GG Cuts at: 3726 3726 Size: 6484 BtgI C'CryG_G Cuts at: 513 1065 2283 2335 2584 2628 2685 2958 3217 Size: 552 1218 52 249 44 57 273 259 Cuts at: 3217 3486 3687 513 Size: 269 201 3310 Fragments arranged by size: 3310 1218 552 273 269 259 249 201 57 52 44 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5745 Fragments arranged by size: 5745 683 56 BtsI GCAGTG_nn' Cuts at: 2044 2105 2391 4000 4794 4814 2044 Size: 61 286 1609 794 20 3714 Fragments arranged by size: 3714 1609 794 286 61 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6484 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6449 DraI TTT'AAA Cuts at: 3788 3788 Size: 6484 DraIII CAC_nnn'GTG Cuts at: 3421 3421 Size: 6484 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2549 5491 809 Size: 507 1233 2942 1802 Fragments arranged by size: 2942 1802 1233 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2061 2565 Size: 54 1425 50 275 18 216 15 504 Cuts at: 2565 2653 2803 2949 3214 3489 3690 3931 4820 Size: 88 150 146 265 275 201 241 889 Cuts at: 4820 6449 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 504 275 275 265 241 216 201 150 146 88 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2046 2565 3489 3690 3931 1537 Size: 275 234 519 924 201 241 4090 Fragments arranged by size: 4090 924 519 275 241 234 201 EarI CTCTTCn'nnn_ Cuts at: 1353 2468 2789 4414 1353 Size: 1115 321 1625 3423 Fragments arranged by size: 3423 1625 1115 321 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2402 5071 5445 5591 387 Size: 1064 138 813 2669 374 146 1280 Fragments arranged by size: 2669 1280 1064 813 374 146 138 EcoICRI GAG'CTC Cuts at: 2395 2395 Size: 6484 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2528 2942 2957 3366 3546 3627 4547 Size: 393 896 414 15 409 180 81 920 Cuts at: 4547 5144 5931 6199 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 920 896 787 597 414 409 393 268 180 81 15 EcoO109I rG'GnC_Cy Cuts at: 2055 2194 3179 3754 2055 Size: 139 985 575 4785 Fragments arranged by size: 4785 985 575 139 EcoRI G'AATT_C Cuts at: 3594 3793 4171 3594 Size: 199 378 5907 Fragments arranged by size: 5907 378 199 EcoRV GAT'ATC Cuts at: 3236 3236 Size: 6484 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2815 2815 Size: 6484 FspI TGC'GCA Cuts at: 2756 4990 2756 Size: 2234 4250 HaeII r_GCGC'y Cuts at: 1678 2117 5631 1678 Size: 439 3514 2531 Fragments arranged by size: 3514 2531 439 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5125 5157 5199 5231 1373 Size: 32 135 32 3553 32 42 32 2626 Fragments arranged by size: 3553 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3307 3528 3828 4074 669 Size: 988 281 1369 221 300 246 3079 Fragments arranged by size: 3079 1369 988 300 281 246 221 HindIII A'AGCT_T Cuts at: 748 748 Size: 6484 HpaI GTT'AAC Cuts at: 4074 4074 Size: 6484 KpnI G_GTAC'C Cuts at: 3809 3809 Size: 6484 MfeI C'AATT_G Cuts at: 4083 4083 Size: 6484 MluI A'CGCG_T Cuts at: 6360 6360 Size: 6484 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5598 5782 1305 Size: 4293 184 2007 Fragments arranged by size: 4293 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2951 10 Size: 54 1425 343 1119 3543 Fragments arranged by size: 3543 1425 1119 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3446 4442 4801 4960 518 Size: 648 2280 996 359 159 2042 Fragments arranged by size: 2280 2042 996 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2337 2586 2665 3161 3488 3509 3689 3900 Size: 731 249 79 496 327 21 180 211 Cuts at: 3900 4577 5725 5970 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 731 677 496 327 249 245 211 180 79 21 NcoI C'CATG_G Cuts at: 513 1065 2628 513 Size: 552 1563 4369 Fragments arranged by size: 4369 1563 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6484 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6484 NotI GC'GGCC_GC Cuts at: 3489 3690 3931 3489 Size: 201 241 6042 Fragments arranged by size: 6042 241 201 NspI r_CATG'y Cuts at: 1132 1229 2894 3842 5387 1132 Size: 97 1665 948 1545 2229 Fragments arranged by size: 2229 1665 1545 948 97 PasI CC'CwG_GG Cuts at: 1348 3176 1348 Size: 1828 4656 PciI A'CATG_T Cuts at: 5383 5383 Size: 6484 PflMI CCAn_nnn'nTGG Cuts at: 1108 3421 1108 Size: 2313 4171 PfoI T'CCnGG_A Cuts at: 2505 2505 Size: 6484 PmeI GTTT'AAAC Cuts at: 3788 3788 Size: 6484 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4553 4585 6066 6098 4553 Size: 32 1481 32 4939 Fragments arranged by size: 4939 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2055 3754 2055 Size: 1699 4785 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6484 PsiI TTA'TAA Cuts at: 4054 4054 Size: 6484 PspOMI G'GGCC_C Cuts at: 2194 3101 2194 Size: 907 5577 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2324 2356 2324 Size: 32 6452 PstI C_TGCA'G Cuts at: 830 2444 3836 830 Size: 1614 1392 3478 Fragments arranged by size: 3478 1614 1392 PvuI CG_AT'CG Cuts at: 2001 3660 2001 Size: 1659 4825 PvuII CAG'CTG Cuts at: 2665 3161 2665 Size: 496 5988 SacI G_AGCT'C Cuts at: 2397 2397 Size: 6484 SacII CC_GC'GG Cuts at: 2338 2587 3489 3690 2338 Size: 249 902 201 5132 Fragments arranged by size: 5132 902 249 201 SalI G'TCGA_C Cuts at: 1936 3526 3826 1936 Size: 1590 300 4594 Fragments arranged by size: 4594 1590 300 SapI GCTCTTCn'nnn_ Cuts at: 2789 2789 Size: 6484 SbfI CC_TGCA'GG Cuts at: 3836 3836 Size: 6484 ScaI AGT'ACT Cuts at: 2975 4732 6444 2975 Size: 1757 1712 3015 Fragments arranged by size: 3015 1757 1712 SexAI A'CCwGG_T Cuts at: 3325 3325 Size: 6484 SfcI C'TryA_G Cuts at: 826 1045 2134 2440 3648 3668 3832 4967 5648 Size: 219 1089 306 1208 20 164 1135 681 Cuts at: 5648 5839 826 Size: 191 1471 Fragments arranged by size: 1471 1208 1135 1089 681 306 219 191 164 20 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6484 SmaI CCC'GGG Cuts at: 1872 2418 3811 1872 Size: 546 1393 4545 Fragments arranged by size: 4545 1393 546 SmlI C'TyrA_G Cuts at: 820 1017 1943 4588 5489 5751 820 Size: 197 926 2645 901 262 1553 Fragments arranged by size: 2645 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4275 493 Size: 3782 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6484 SphI G_CATG'C Cuts at: 1132 2894 3842 1132 Size: 1762 948 3774 Fragments arranged by size: 3774 1762 948 SrfI GCCC'GGGC Cuts at: 2418 2418 Size: 6484 SspI AAT'ATT Cuts at: 5 52 4408 5 Size: 47 4356 2081 Fragments arranged by size: 4356 2081 47 StuI AGG'CCT Cuts at: 1517 3731 1517 Size: 2214 4270 StyI C'CwwG_G Cuts at: 513 1065 2064 2520 2628 3182 3923 513 Size: 552 999 456 108 554 741 3074 Fragments arranged by size: 3074 999 741 554 552 456 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4511 4528 4681 4866 5311 325 Size: 1255 2931 17 153 185 445 1498 Fragments arranged by size: 2931 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2355 2973 3042 Size: 274 80 33 51 1443 378 618 69 Cuts at: 3042 4730 6442 96 Size: 1688 1712 138 Fragments arranged by size: 1712 1688 1443 618 378 274 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2915 2947 3525 3557 3562 3594 6444 6476 2915 Size: 32 578 32 5 32 2850 32 2923 Fragments arranged by size: 2923 2850 578 32 32 32 32 5 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6484 XbaI T'CTAG_A Cuts at: 3820 3820 Size: 6484 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2184 2184 Size: 6484 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6484 XmaI C'CCGG_G Cuts at: 1870 2416 3809 1870 Size: 546 1393 4545 Fragments arranged by size: 4545 1393 546 XmnI GAAnn'nnTTC Cuts at: 2798 4613 2798 Size: 1815 4669 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3769 4292 276 Size: 53 83 186 936 2235 523 2468 Fragments arranged by size: 2468 2235 936 523 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI BaeI BbeI BfrBI BsiWI BsmBI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SanDI SfiI SfoI SwaI