(Linear) (Six-base) MAPSORT of: KIEE5731.seq Check: 8527 from: 1 to: 1779 KIEE5731.orf With 182 enzymes: SgfI * December 5, 2014 13:08 .. AatII G_ACGT'C Cuts at: 0 1766 1779 Size: 1766 13 AccI GT'mk_AC Cuts at: 0 548 1522 1562 1766 1779 Size: 548 974 40 204 13 Fragments arranged by size: 974 548 204 40 13 AclI AA'CG_TT Cuts at: 0 1580 1779 Size: 1580 199 AflIII A'CryG_T Cuts at: 0 242 1034 1779 Size: 242 792 745 Fragments arranged by size: 792 745 242 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 658 1779 Size: 658 1121 AlwNI CAG_nnn'CTG Cuts at: 0 1504 1779 Size: 1504 275 ApaI G_GGCC'C Cuts at: 0 193 1100 1779 Size: 193 907 679 Fragments arranged by size: 907 679 193 ApaLI G'TGCA_C Cuts at: 0 856 1779 Size: 856 923 ApoI r'AATT_y Cuts at: 0 1281 1589 1779 Size: 1281 308 190 Fragments arranged by size: 1281 308 190 AscI GG'CGCG_CC Cuts at: 0 83 1779 Size: 83 1696 AvaI C'yCGr_G Cuts at: 0 67 411 1192 1779 Size: 67 344 781 587 Fragments arranged by size: 781 587 344 67 AvrII C'CTAG_G Cuts at: 0 1177 1779 Size: 1177 602 BamHI G'GATC_C Cuts at: 0 470 1779 Size: 470 1309 BanI G'GyrC_C Cuts at: 0 63 261 1275 1779 Size: 63 198 1014 504 Fragments arranged by size: 1014 504 198 63 BanII G_rGCy'C Cuts at: 0 193 392 925 1100 1170 1353 1779 Size: 193 199 533 175 70 183 426 Fragments arranged by size: 533 426 199 193 183 175 70 BbsI GAAGACnn'nnnn_ Cuts at: 0 210 1779 Size: 210 1569 BbvCI CC'TCA_GC Cuts at: 0 1010 1779 Size: 1010 769 BciVI GTATCCnnnnn_n' Cuts at: 0 1696 1779 Size: 1696 83 BclI T'GATC_A Cuts at: 0 1427 1779 Size: 1427 352 BglI GCCn_nnn'nGGC Cuts at: 0 425 559 1348 1779 Size: 425 134 789 431 Fragments arranged by size: 789 431 425 134 Bme1580I G_kGCm'C Cuts at: 0 68 193 266 860 1100 1779 Size: 68 125 73 594 240 679 Fragments arranged by size: 679 594 240 125 73 68 BmgBI CAC'GTC Cuts at: 0 243 540 708 720 1779 Size: 243 297 168 12 1059 Fragments arranged by size: 1059 297 243 168 12 BmrI ACTGGGnnnn_n' Cuts at: 0 696 1300 1779 Size: 696 604 479 Fragments arranged by size: 696 604 479 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 987 1779 Size: 987 792 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 523 937 952 1361 1541 1622 1779 Size: 523 414 15 409 180 81 157 Fragments arranged by size: 523 414 409 180 157 81 15 Bpu10I CC'TnA_GC Cuts at: 0 1010 1779 Size: 1010 769 BsaI GGTCTCn'nnnn_ Cuts at: 0 721 760 1779 Size: 721 39 1019 Fragments arranged by size: 1019 721 39 BsaAI yAC'GTr Cuts at: 0 747 1035 1623 1779 Size: 747 288 588 156 Fragments arranged by size: 747 588 288 156 BsaHI Gr'CG_yC Cuts at: 0 1525 1763 1779 Size: 1525 238 16 Fragments arranged by size: 1525 238 16 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 910 940 1024 1054 1557 1587 1779 Size: 910 30 84 30 503 30 192 Fragments arranged by size: 910 503 192 84 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 520 1779 Size: 520 1259 BseYI C'CCAG_C Cuts at: 0 182 1027 1455 1779 Size: 182 845 428 324 Fragments arranged by size: 845 428 324 182 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1172 1465 1779 Size: 1172 293 314 Fragments arranged by size: 1172 314 293 BsiEI CG_ry'CG Cuts at: 0 44 563 1487 1655 1688 1779 Size: 44 519 924 168 33 91 Fragments arranged by size: 924 519 168 91 44 33 BsiHKAI G_wGCw'C Cuts at: 0 392 860 1424 1779 Size: 392 468 564 355 Fragments arranged by size: 564 468 392 355 Bsp1286I G_dGCh'C Cuts at: 0 68 193 266 392 860 925 1100 1170 Size: 68 125 73 126 468 65 175 70 Cuts at: 1170 1353 1424 1779 Size: 183 71 355 Fragments arranged by size: 468 355 183 175 126 125 73 71 70 68 65 BspHI T'CATG_A Cuts at: 0 305 758 1233 1779 Size: 305 453 475 546 Fragments arranged by size: 546 475 453 305 BsrDI GCAATG_nn' Cuts at: 0 1436 1779 Size: 1436 343 BssHII G'CGCG_C Cuts at: 0 83 375 1779 Size: 83 292 1404 Fragments arranged by size: 1404 292 83 BssSI C'ACGA_G Cuts at: 0 364 924 1779 Size: 364 560 855 Fragments arranged by size: 855 560 364 BstEII G'GTnAC_C Cuts at: 0 519 1779 Size: 519 1260 BstXI CCAn_nnnn'nTGG Cuts at: 0 954 1779 Size: 954 825 BstYI r'GATC_y Cuts at: 0 230 404 470 737 836 1604 1779 Size: 230 174 66 267 99 768 175 Fragments arranged by size: 768 267 230 175 174 99 66 BstZ17I GTA'TAC Cuts at: 0 1563 1779 Size: 1563 216 Bsu36I CC'TnA_GG Cuts at: 0 1721 1779 Size: 1721 58 BtgI C'CryG_G Cuts at: 0 278 330 579 623 680 953 1212 1481 Size: 278 52 249 44 57 273 259 269 Cuts at: 1481 1682 1779 Size: 201 97 Fragments arranged by size: 278 273 269 259 249 201 97 57 52 44 BtsI GCAGTG_nn' Cuts at: 0 39 100 386 1779 Size: 39 61 286 1393 Fragments arranged by size: 1393 286 61 39 DraIII CAC_nnn'GTG Cuts at: 0 1416 1779 Size: 1416 363 DrdI GACnn_nn'nnGTC Cuts at: 0 544 1779 Size: 544 1235 EaeI y'GGCC_r Cuts at: 0 41 56 560 648 798 944 1209 1484 Size: 41 15 504 88 150 146 265 275 Cuts at: 1484 1685 1779 Size: 201 94 Fragments arranged by size: 504 275 265 201 150 146 94 88 41 15 EagI C'GGCC_G Cuts at: 0 41 560 1484 1685 1779 Size: 41 519 924 201 94 Fragments arranged by size: 924 519 201 94 41 EarI CTCTTCn'nnn_ Cuts at: 0 463 784 1779 Size: 463 321 995 Fragments arranged by size: 995 463 321 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 397 1779 Size: 397 1382 EcoICRI GAG'CTC Cuts at: 0 390 1779 Size: 390 1389 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 523 937 952 1361 1541 1622 1779 Size: 523 414 15 409 180 81 157 Fragments arranged by size: 523 414 409 180 157 81 15 EcoO109I rG'GnC_Cy Cuts at: 0 50 189 1174 1749 1779 Size: 50 139 985 575 30 Fragments arranged by size: 985 575 139 50 30 EcoRI G'AATT_C Cuts at: 0 1589 1779 Size: 1589 190 EcoRV GAT'ATC Cuts at: 0 1231 1779 Size: 1231 548 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 810 1779 Size: 810 969 FspI TGC'GCA Cuts at: 0 751 1779 Size: 751 1028 HaeII r_GCGC'y Cuts at: 0 112 1779 Size: 112 1667 HincII GTy'rAC Cuts at: 0 1302 1523 1779 Size: 1302 221 256 Fragments arranged by size: 1302 256 221 MscI TGG'CCA Cuts at: 0 946 1779 Size: 946 833 MslI CAynn'nnrTG Cuts at: 0 1441 1779 Size: 1441 338 MspA1I CmG'CkG Cuts at: 0 332 581 660 1156 1483 1504 1684 1779 Size: 332 249 79 496 327 21 180 95 Fragments arranged by size: 496 332 327 249 180 95 79 21 NcoI C'CATG_G Cuts at: 0 623 1779 Size: 623 1156 NotI GC'GGCC_GC Cuts at: 0 1484 1685 1779 Size: 1484 201 94 Fragments arranged by size: 1484 201 94 NspI r_CATG'y Cuts at: 0 889 1779 Size: 889 890 PasI CC'CwG_GG Cuts at: 0 1171 1779 Size: 1171 608 PflMI CCAn_nnn'nTGG Cuts at: 0 1416 1779 Size: 1416 363 PfoI T'CCnGG_A Cuts at: 0 500 1779 Size: 500 1279 PpuMI rG'GwC_Cy Cuts at: 0 50 1749 1779 Size: 50 1699 30 Fragments arranged by size: 1699 50 30 PspOMI G'GGCC_C Cuts at: 0 189 1096 1779 Size: 189 907 683 Fragments arranged by size: 907 683 189 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 319 351 1779 Size: 319 32 1428 Fragments arranged by size: 1428 319 32 PstI C_TGCA'G Cuts at: 0 439 1779 Size: 439 1340 PvuI CG_AT'CG Cuts at: 0 1655 1779 Size: 1655 124 PvuII CAG'CTG Cuts at: 0 660 1156 1779 Size: 660 496 623 Fragments arranged by size: 660 623 496 SacI G_AGCT'C Cuts at: 0 392 1779 Size: 392 1387 SacII CC_GC'GG Cuts at: 0 333 582 1484 1685 1779 Size: 333 249 902 201 94 Fragments arranged by size: 902 333 249 201 94 SalI G'TCGA_C Cuts at: 0 1521 1779 Size: 1521 258 SapI GCTCTTCn'nnn_ Cuts at: 0 784 1779 Size: 784 995 ScaI AGT'ACT Cuts at: 0 970 1779 Size: 970 809 SexAI A'CCwGG_T Cuts at: 0 1320 1779 Size: 1320 459 SfcI C'TryA_G Cuts at: 0 129 435 1643 1663 1779 Size: 129 306 1208 20 116 Fragments arranged by size: 1208 306 129 116 20 SmaI CCC'GGG Cuts at: 0 413 1779 Size: 413 1366 SphI G_CATG'C Cuts at: 0 889 1779 Size: 889 890 SrfI GCCC'GGGC Cuts at: 0 413 1779 Size: 413 1366 StuI AGG'CCT Cuts at: 0 1726 1779 Size: 1726 53 StyI C'CwwG_G Cuts at: 0 59 515 623 1177 1779 Size: 59 456 108 554 602 Fragments arranged by size: 602 554 456 108 59 TatI w'GTAC_w Cuts at: 0 350 968 1037 1779 Size: 350 618 69 742 Fragments arranged by size: 742 618 350 69 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 910 942 1520 1552 1557 1589 1779 Size: 910 32 578 32 5 32 190 Fragments arranged by size: 910 578 190 32 32 32 5 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 179 1779 Size: 179 1600 XmaI C'CCGG_G Cuts at: 0 411 1779 Size: 411 1368 XmnI GAAnn'nnTTC Cuts at: 0 793 1779 Size: 793 986 ZraI GAC'GTC Cuts at: 0 1764 1779 Size: 1764 15 Enzymes that do cut: AatII AccI AclI AflIII AlfI AlwNI ApaI ApaLI ApoI AscI AvaI AvrII BamHI BanI BanII BbsI BbvCI BciVI BclI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrDI BssHII BssSI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII HincII MscI MslI MspA1I NcoI NotI NspI PasI PflMI PfoI PpuMI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SapI ScaI SexAI SfcI SmaI SphI SrfI StuI StyI TatI TstI XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI Acc65I AcuI AfeI AflII AgeI AhdI AleI AloI AseI AsiSI BaeI BbeI BcgI BfrBI BglII BlpI BmtI BpuEI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BstAPI BstBI BtgZI ClaI CspCI DraI EcoNI FseI FspAI Hin4I HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI RsrII SanDI SbfI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SspI SwaI TaqII Tth111I XbaI XhoI