(Circular) (Six-base) MAPSORT of: pFN21AE4129.seq Check: 6252 from: 1 to: 6283 pFN21AE4129 6283 bp With 182 enzymes: SgfI * March 28, 2012 13:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4093 278 Size: 53 83 186 936 2557 2468 Fragments arranged by size: 2557 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2280 3626 1937 Size: 343 1346 4594 Fragments arranged by size: 4594 1346 343 Acc65I G'GTAC_C Cuts at: 3604 3604 Size: 6283 AclI AA'CG_TT Cuts at: 1563 4410 4783 1563 Size: 2847 373 3063 Fragments arranged by size: 3063 2847 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 2177 3184 3528 3539 4346 5730 5998 Size: 464 81 1007 344 11 807 1384 268 Cuts at: 5998 1632 Size: 1917 Fragments arranged by size: 1917 1384 1007 807 464 344 268 81 11 AflII C'TTAA_G Cuts at: 820 1017 2843 820 Size: 197 1826 4260 Fragments arranged by size: 4260 1826 197 AflIII A'CryG_T Cuts at: 1203 5182 6159 1203 Size: 3979 977 1327 Fragments arranged by size: 3979 1327 977 AgeI A'CCGG_T Cuts at: 5114 5114 Size: 6283 AhdI GACnn_n'nnGTC Cuts at: 5012 5012 Size: 6283 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6283 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6283 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2340 2372 2340 Size: 32 6251 AlwNI CAG_nnn'CTG Cuts at: 4026 5598 4026 Size: 1572 4711 ApaLI G'TGCA_C Cuts at: 2341 4340 5496 2341 Size: 1999 1156 3128 Fragments arranged by size: 3128 1999 1156 ApoI r'AATT_y Cuts at: 1493 2159 2316 3429 3592 3818 3970 6079 1493 Size: 666 157 1113 163 226 152 2109 1697 Fragments arranged by size: 2109 1697 1113 666 226 163 157 152 AseI AT'TA_AT Cuts at: 160 1032 2489 2493 4837 160 Size: 872 1457 4 2344 1606 Fragments arranged by size: 2344 1606 1457 872 4 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6283 AvaI C'yCGr_G Cuts at: 1870 1943 2976 3608 1870 Size: 73 1033 632 4545 Fragments arranged by size: 4545 1033 632 73 AvrII C'CTAG_G Cuts at: 3409 3409 Size: 6283 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2973 3006 2973 Size: 33 6250 BamHI G'GATC_C Cuts at: 3613 3980 3613 Size: 367 5916 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3604 5059 618 Size: 325 217 627 1817 1455 1842 Fragments arranged by size: 1842 1817 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2069 2791 3319 3602 1391 Size: 678 722 528 283 4072 Fragments arranged by size: 4072 722 678 528 283 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2102 928 Size: 971 203 5109 Fragments arranged by size: 5109 971 203 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4474 4508 1903 Size: 34 2537 34 3678 Fragments arranged by size: 3678 2537 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2366 2974 3359 3407 4175 5385 2366 Size: 608 385 48 768 1210 3264 Fragments arranged by size: 3264 1210 768 608 385 48 BclI T'GATC_A Cuts at: 1548 1548 Size: 6283 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4894 136 Size: 107 122 71 631 605 3222 1525 Fragments arranged by size: 3222 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6278 6278 Size: 6283 BlpI GC'TnA_GC Cuts at: 3700 3700 Size: 6283 Bme1580I G_kGCm'C Cuts at: 1790 2345 4344 5500 1790 Size: 555 1999 1156 2573 Fragments arranged by size: 2573 1999 1156 555 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6283 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2645 4014 4972 Size: 416 53 83 399 24 1228 1369 958 Cuts at: 4972 442 Size: 1753 Fragments arranged by size: 1753 1369 1228 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6283 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4943 1239 Size: 3704 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2045 2501 4408 5273 5571 2045 Size: 456 1907 865 298 2757 Fragments arranged by size: 2757 1907 865 456 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2110 4946 882 Size: 617 611 2836 2219 Fragments arranged by size: 2836 2219 617 611 BsaAI yAC'GTr Cuts at: 493 1204 2693 4074 493 Size: 711 1489 1381 2702 Fragments arranged by size: 2702 1489 1381 711 BsaBI GATnn'nnATC Cuts at: 6277 6277 Size: 6283 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4090 4472 5107 275 Size: 53 83 186 936 2557 382 635 1451 Fragments arranged by size: 2557 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4716 5114 5388 5535 5946 4716 Size: 398 274 147 411 5053 Fragments arranged by size: 5053 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6058 Fragments arranged by size: 6058 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 1186 Size: 844 5439 BseYI C'CCAG_C Cuts at: 5486 5982 5486 Size: 496 5787 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2232 1163 Size: 573 496 5214 Fragments arranged by size: 5214 573 496 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3733 4494 5522 1540 Size: 275 186 1732 761 1028 2301 Fragments arranged by size: 2301 1732 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2345 4344 4429 5500 2345 Size: 1999 85 1071 3128 Fragments arranged by size: 3128 1999 1071 85 BsmI GAATG_Cn' Cuts at: 2203 2606 3005 3794 3887 2203 Size: 403 399 789 93 4599 Fragments arranged by size: 4599 789 403 399 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2345 2791 3319 3602 4344 4429 Size: 399 279 276 446 528 283 742 85 Cuts at: 4429 5500 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 528 446 399 283 279 276 85 BspEI T'CCGG_A Cuts at: 5946 5946 Size: 6283 BspHI T'CATG_A Cuts at: 4172 5902 4172 Size: 1730 4553 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3638 844 Size: 2794 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4170 1394 Size: 429 2347 3507 Fragments arranged by size: 3507 2347 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2923 4778 4952 65 Size: 1183 1675 1855 174 1396 Fragments arranged by size: 1855 1675 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4927 5114 1700 Size: 110 3117 187 2869 Fragments arranged by size: 3117 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6283 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6283 BssSI C'ACGA_G Cuts at: 4343 5355 4343 Size: 1012 5271 BstAPI GCAn_nnn'nTGC Cuts at: 794 3518 794 Size: 2724 3559 BstBI TT'CG_AA Cuts at: 5087 5087 Size: 6283 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6283 BstYI r'GATC_y Cuts at: 1972 3613 3980 4365 4382 5834 5920 5932 6278 Size: 1641 367 385 17 1452 86 12 346 Cuts at: 6278 1972 Size: 1977 Fragments arranged by size: 1977 1641 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2281 2281 Size: 6283 Bsu36I CC'TnA_GG Cuts at: 2469 2817 2469 Size: 348 5935 BtgI C'CryG_G Cuts at: 513 1065 2763 513 Size: 552 1698 4033 Fragments arranged by size: 4033 1698 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5544 Fragments arranged by size: 5544 683 56 BtsI GCAGTG_nn' Cuts at: 3477 3799 4593 4613 3477 Size: 322 794 20 5147 Fragments arranged by size: 5147 794 322 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6283 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6248 DraI TTT'AAA Cuts at: 2167 2331 3537 3587 2167 Size: 164 1206 50 4863 Fragments arranged by size: 4863 1206 164 50 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2757 5290 809 Size: 507 1441 2533 1802 Fragments arranged by size: 2533 1802 1441 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3730 4619 6248 Size: 54 1425 50 275 18 1900 889 1629 Cuts at: 6248 8 Size: 43 Fragments arranged by size: 1900 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3730 1537 Size: 275 1918 4090 Fragments arranged by size: 4090 1918 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2318 3223 3270 3555 4213 1353 Size: 965 905 47 285 658 3423 Fragments arranged by size: 3423 965 905 658 285 47 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4870 5244 5390 387 Size: 1064 138 3281 374 146 1280 Fragments arranged by size: 3281 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2177 3184 3528 3539 4346 4943 Size: 393 464 81 1007 344 11 807 597 Cuts at: 4943 5730 5998 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1007 807 787 597 464 393 344 268 81 11 EcoNI CCTnn'n_nnAGG Cuts at: 3482 3482 Size: 6283 EcoRI G'AATT_C Cuts at: 2159 3592 3970 2159 Size: 1433 378 4472 Fragments arranged by size: 4472 1433 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2179 2417 3533 2179 Size: 238 1116 4929 Fragments arranged by size: 4929 1116 238 FspI TGC'GCA Cuts at: 4789 4789 Size: 6283 HaeII r_GCGC'y Cuts at: 1678 5430 1678 Size: 3752 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3476 3508 4924 4956 4998 Size: 32 135 32 1904 32 1416 32 42 Cuts at: 4998 5030 1373 Size: 32 2626 Fragments arranged by size: 2626 1904 1416 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2907 3627 3873 669 Size: 988 281 969 720 246 3079 Fragments arranged by size: 3079 988 969 720 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6283 HpaI GTT'AAC Cuts at: 3873 3873 Size: 6283 KpnI G_GTAC'C Cuts at: 3608 3608 Size: 6283 MfeI C'AATT_G Cuts at: 3882 3882 Size: 6283 MluI A'CGCG_T Cuts at: 6159 6159 Size: 6283 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2001 5397 5581 1305 Size: 696 3396 184 2007 Fragments arranged by size: 3396 2007 696 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4461 Fragments arranged by size: 4461 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3340 4241 4600 4759 518 Size: 648 2174 901 359 159 2042 Fragments arranged by size: 2174 2042 901 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2086 3449 3506 3699 4376 5524 5769 1606 Size: 480 1363 57 193 677 1148 245 2120 Fragments arranged by size: 2120 1363 1148 677 480 245 193 57 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5731 NdeI CA'TA_TG Cuts at: 387 387 Size: 6283 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6283 NotI GC'GGCC_GC Cuts at: 3730 3730 Size: 6283 NspI r_CATG'y Cuts at: 1132 1229 2690 3641 5186 1132 Size: 97 1461 951 1545 2229 Fragments arranged by size: 2229 1545 1461 951 97 PacI TTA_AT'TAA Cuts at: 2493 2493 Size: 6283 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6283 PciI A'CATG_T Cuts at: 5182 5182 Size: 6283 PflMI CCAn_nnn'nTGG Cuts at: 1108 3086 1108 Size: 1978 4305 PmeI GTTT'AAAC Cuts at: 3587 3587 Size: 6283 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4352 4384 5865 5897 4352 Size: 32 1481 32 4738 Fragments arranged by size: 4738 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6283 PsiI TTA'TAA Cuts at: 3853 3853 Size: 6283 PstI C_TGCA'G Cuts at: 830 3505 3635 830 Size: 2675 130 3478 Fragments arranged by size: 3478 2675 130 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6283 PvuII CAG'CTG Cuts at: 2086 3449 3506 2086 Size: 1363 57 4863 Fragments arranged by size: 4863 1363 57 SalI G'TCGA_C Cuts at: 1936 3625 1936 Size: 1689 4594 SbfI CC_TGCA'GG Cuts at: 3635 3635 Size: 6283 ScaI AGT'ACT Cuts at: 4531 6243 4531 Size: 1712 4571 SfcI C'TryA_G Cuts at: 826 1045 3375 3501 3631 4766 5447 5638 826 Size: 219 2330 126 130 1135 681 191 1471 Fragments arranged by size: 2330 1471 1135 681 219 191 130 126 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6283 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6283 SmaI CCC'GGG Cuts at: 1872 2978 3610 1872 Size: 1106 632 4545 Fragments arranged by size: 4545 1106 632 SmlI C'TyrA_G Cuts at: 820 1017 1943 2060 2480 2843 4387 5288 5550 Size: 197 926 117 420 363 1544 901 262 Cuts at: 5550 820 Size: 1553 Fragments arranged by size: 1553 1544 926 901 420 363 262 197 117 SnaBI TAC'GTA Cuts at: 493 2693 4074 493 Size: 2200 1381 2702 Fragments arranged by size: 2702 2200 1381 SpeI A'CTAG_T Cuts at: 152 152 Size: 6283 SphI G_CATG'C Cuts at: 1132 3641 1132 Size: 2509 3774 SspI AAT'ATT Cuts at: 5 52 2803 4207 5 Size: 47 2751 1404 2081 Fragments arranged by size: 2751 2081 1404 47 StuI AGG'CCT Cuts at: 1517 2099 1517 Size: 582 5701 StyI C'CwwG_G Cuts at: 513 1065 3409 3722 513 Size: 552 2344 313 3074 Fragments arranged by size: 3074 2344 552 313 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4310 4327 4480 4665 5110 325 Size: 1255 2730 17 153 185 445 1498 Fragments arranged by size: 2730 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2076 2742 4529 Size: 274 80 33 51 1443 99 666 1787 Cuts at: 4529 6241 96 Size: 1712 138 Fragments arranged by size: 1787 1712 1443 666 274 138 99 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6243 6275 6243 Size: 32 6251 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6283 XbaI T'CTAG_A Cuts at: 3619 3619 Size: 6283 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6283 XmaI C'CCGG_G Cuts at: 1870 2976 3608 1870 Size: 1106 632 4545 Fragments arranged by size: 4545 1106 632 XmnI GAAnn'nnTTC Cuts at: 3264 3513 4412 3264 Size: 249 899 5135 Fragments arranged by size: 5135 899 249 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4091 276 Size: 53 83 186 936 2557 2468 Fragments arranged by size: 2557 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI