Restriction map for
pFN21AE2419
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BfaI BfrI
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV FseI FspI Hin1I HindIII
HpaI KasI KspI MaeI MfeI
MroI MunI NarI NcoI NdeI
NheI NotI NruI NspI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 1505 GGTACC G^GTACC
AcsI 1065 GAATTT [GA]^AATT[CT]
AflIII 1740 ACGCGT A^C[GA][CT]GT
ApoI 1065 GAATTT [GA]^AATT[CT]
Asp718I 1505 GGTACC G^GTACC
BcnI 1077 CCCGG CC^[GC]GG
BglI 1405 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 783 AGATCT A^GATCT
BsaBI 1329 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 1818 CGTCTC CGTCTC(1/5)
BsmI 2056 GAATGC GAATGC(1/-1)
BspHI 1142 TCATGA T^CATGA
BstEII 456 GGTGACC G^GT[ACGT]ACC
BstPI 456 GGTGACC G^GT[ACGT]ACC
DraII 2030 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 2030 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 456 GGTGACC G^GT[ACGT]ACC
EcoT22I 1436 ATGCAT ATGCA^T
HaeII 358 AGCGCC [GA]GCGC^[CT]
HgaI 1742 GCGTC GACGC(5/10)
HincII 1239 GTTGAC GT[CT]^[GA]AC
HindII 1239 GTTGAC GT[CT]^[GA]AC
KpnI 1505 GGTACC GGTAC^C
MamI 1329 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1740 ACGCGT A^CGCGT
MseI 935 TTAA T^TAA
NaeI 1648 GCCGGC GCC^GGC
NciI 1077 CCCGG CC^[GC]GG
NgoMIV 1648 GCCGGC G^CCGGC
NsiI 1436 ATGCAT ATGCA^T
PaeR7I 721 CTCGAG C^TCGAG
PpuMI 2030 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 1142 TCATGA T^CATGA
SacI 474 GAGCTC GAGCT^C
SapI 632 GAAGAGC GCTCTTC(1/4)
Tru9I 935 TTAA T^TAA
XhoI 721 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AvaI 231 CTCGGG C^[CT]CG[GA]G
721 CTCGAG
BclI 1139 TGATCA T^GATCA
1598 TGATCA
BsaI 100 GAGACC GGTCTC(1/5)
1987 GAGACC
BseRI 1443 GAGGAG GAGGAG(10/8)
1945 GAGGAG
BsgI 963 CTGCAC GTGCAG(16/14)
469 GTGCAG
BsmFI 1969 GTCCC GGGAC(10/14)
2032 GTCCC
BsoBI 231 CTCGGG C^[CT]CG[GA]G
721 CTCGAG
BsrFI 1648 GCCGGC [GA]^CCGG[CT]
1831 ACCGGC
BstAPI 1922 GCAGCAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1925 GCAAGTGCTGC
Cfr10I 1648 GCCGGC [GA]^CCGG[CT]
1831 ACCGGC
DsaI 164 CCACGG C^C[GA][CT]GG
698 CCGTGG
EcoT14I 285 CCAAGG C^C[AT][AT]GG
1544 CCAAGG
FauI 1620 CCCGC CCCGC(4/6)
1656 GCGGG
FbaI 1139 TGATCA T^GATCA
1598 TGATCA
PstI 1723 CTGCAG CTGCA^G
1920 CTGCAG
PvuII 367 CAGCTG CAG^CTG
829 CAGCTG
SfcI 1723 CTGCAG C^T[GA][CT]AG
1920 CTGCAG
SmlI 721 CTCGAG C^T[CT][GA]AG
1171 CTCAAG
StyI 285 CCAAGG C^C[AT][AT]GG
1544 CCAAGG
TfiI 270 GATTC G^A[AT]TC
1334 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BalI BanI BanII
BbsI BbvI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EarI EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII