(Circular) (Six-base) MAPSORT of: pFN21AE2419.seq Check: 7076 from: 1 to: 6775 pFN21AE2419 6775 bp With 182 enzymes: SgfI * August 14, 2012 13:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2602 2602 Size: 6775 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4585 278 Size: 53 83 186 936 3049 2468 Fragments arranged by size: 3049 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4118 1937 Size: 2181 4594 Acc65I G'GTAC_C Cuts at: 3510 4096 3510 Size: 586 6189 AclI AA'CG_TT Cuts at: 1563 4902 5275 1563 Size: 3339 373 3063 Fragments arranged by size: 3339 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2213 2389 3824 4838 6222 6490 1632 Size: 581 176 1435 1014 1384 268 1917 Fragments arranged by size: 1917 1435 1384 1014 581 268 176 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6578 AflIII A'CryG_T Cuts at: 1203 3745 5674 6651 1203 Size: 2542 1929 977 1327 Fragments arranged by size: 2542 1929 1327 977 AgeI A'CCGG_T Cuts at: 5606 5606 Size: 6775 AhdI GACnn_n'nnGTC Cuts at: 5504 5504 Size: 6775 AleI CACnn'nnGTG Cuts at: 1166 2198 1166 Size: 1032 5743 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2100 3122 1184 Size: 916 1022 4837 Fragments arranged by size: 4837 1022 916 AlwNI CAG_nnn'CTG Cuts at: 4518 6090 4518 Size: 1572 5203 ApaLI G'TGCA_C Cuts at: 4832 5988 4832 Size: 1156 5619 ApoI r'AATT_y Cuts at: 1493 3070 4084 4310 4462 6571 1493 Size: 1577 1014 226 152 2109 1697 Fragments arranged by size: 2109 1697 1577 1014 226 152 AseI AT'TA_AT Cuts at: 160 1032 5329 160 Size: 872 4297 1606 Fragments arranged by size: 4297 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6775 AvaI C'yCGr_G Cuts at: 1870 1943 2236 2726 4100 1870 Size: 73 293 490 1374 4545 Fragments arranged by size: 4545 1374 490 293 73 BamHI G'GATC_C Cuts at: 4105 4472 4105 Size: 367 6408 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2131 3098 3395 3510 4096 Size: 325 217 627 344 967 297 115 586 Cuts at: 4096 5551 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 967 627 586 344 325 297 217 115 BanII G_rGCy'C Cuts at: 1391 2193 2483 3170 3386 4094 1391 Size: 802 290 687 216 708 4072 Fragments arranged by size: 4072 802 708 687 290 216 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3212 3586 3685 928 Size: 971 1313 374 99 4018 Fragments arranged by size: 4018 1313 971 374 99 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4966 5000 1903 Size: 34 3029 34 3678 Fragments arranged by size: 3678 3029 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4667 5877 4667 Size: 1210 5565 BclI T'GATC_A Cuts at: 1548 3144 3603 1548 Size: 1596 459 4720 Fragments arranged by size: 4720 1596 459 BfrBI ATG'CAT Cuts at: 3443 3443 Size: 6775 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3416 5386 136 Size: 107 122 71 631 605 1744 1970 1525 Fragments arranged by size: 1970 1744 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2788 6770 2788 Size: 3982 2793 BlpI GC'TnA_GC Cuts at: 4192 4192 Size: 6775 Bme1580I G_kGCm'C Cuts at: 1790 3101 4836 5992 1790 Size: 1311 1735 1156 2573 Fragments arranged by size: 2573 1735 1311 1156 BmgBI CAC'GTC Cuts at: 1318 3315 1318 Size: 1997 4778 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4506 5464 442 Size: 416 53 83 399 24 3089 958 1753 Fragments arranged by size: 3089 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6775 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2467 3443 3749 5435 1239 Size: 1228 976 306 1686 2579 Fragments arranged by size: 2579 1686 1228 976 306 Bpu10I CC'TnA_GC Cuts at: 3424 4026 3424 Size: 602 6173 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3161 4900 5765 6063 3161 Size: 1739 865 298 3873 Fragments arranged by size: 3873 1739 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2099 3986 5438 882 Size: 617 600 1887 1452 2219 Fragments arranged by size: 2219 1887 1452 617 600 BsaAI yAC'GTr Cuts at: 493 1204 4566 493 Size: 711 3362 2702 Fragments arranged by size: 3362 2702 711 BsaBI GATnn'nnATC Cuts at: 3338 6769 3338 Size: 3431 3344 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4582 4964 5599 275 Size: 53 83 186 936 3049 382 635 1451 Fragments arranged by size: 3049 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5208 5606 5880 6027 6438 5208 Size: 398 274 147 411 5545 Fragments arranged by size: 5545 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2643 2673 2957 2987 3702 Size: 30 165 30 1240 30 284 30 715 Cuts at: 3702 3732 1178 Size: 30 4221 Fragments arranged by size: 4221 1240 715 284 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3463 3965 1186 Size: 2277 502 3996 Fragments arranged by size: 3996 2277 502 BseYI C'CCAG_C Cuts at: 2034 2739 3379 3556 5978 6474 2034 Size: 705 640 177 2422 496 2335 Fragments arranged by size: 2422 2335 705 640 496 177 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2495 2953 1163 Size: 573 759 458 4985 Fragments arranged by size: 4985 759 573 458 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4225 4986 6014 1540 Size: 275 186 2224 761 1028 2301 Fragments arranged by size: 2301 2224 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2345 2483 2697 3177 4836 4921 5992 2345 Size: 138 214 480 1659 85 1071 3128 Fragments arranged by size: 3128 1659 1071 480 214 138 85 BsmI GAATG_Cn' Cuts at: 4067 4286 4379 4067 Size: 219 93 6463 Fragments arranged by size: 6463 219 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3829 3829 Size: 6775 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2193 2345 2483 2697 3101 3170 3177 Size: 399 403 152 138 214 404 69 7 Cuts at: 3177 3386 4094 4836 4921 5992 1391 Size: 209 708 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 708 404 403 399 214 209 152 138 85 69 7 BspEI T'CCGG_A Cuts at: 6438 6438 Size: 6775 BspHI T'CATG_A Cuts at: 3147 4664 6394 3147 Size: 1517 1730 3528 Fragments arranged by size: 3528 1730 1517 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2060 2602 3175 4130 844 Size: 1216 542 573 955 3489 Fragments arranged by size: 3489 1216 955 573 542 BsrBI CCG'CTC Cuts at: 1394 1823 4662 1394 Size: 429 2839 3507 Fragments arranged by size: 3507 2839 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2525 2871 3445 5270 5444 65 Size: 1183 1277 346 574 1825 174 1396 Fragments arranged by size: 1825 1396 1277 1183 574 346 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3653 3836 5419 5606 1700 Size: 110 1843 183 1583 187 2869 Fragments arranged by size: 2869 1843 1583 187 183 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6775 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6775 BssSI C'ACGA_G Cuts at: 4835 5847 4835 Size: 1012 5763 BstAPI GCAn_nnn'nTGC Cuts at: 794 3933 3936 794 Size: 3139 3 3633 Fragments arranged by size: 3633 3139 3 BstBI TT'CG_AA Cuts at: 5579 5579 Size: 6775 BstEII G'GTnAC_C Cuts at: 1184 2461 1184 Size: 1277 5498 BstXI CCAn_nnnn'nTGG Cuts at: 2200 2297 2378 2560 3544 3556 2200 Size: 97 81 182 984 12 5419 Fragments arranged by size: 5419 984 182 97 81 12 BstYI r'GATC_y Cuts at: 1972 2788 3085 3159 4105 4472 4857 4874 6326 Size: 816 297 74 946 367 385 17 1452 Cuts at: 6326 6412 6424 6770 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 946 816 385 367 346 297 86 74 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2169 2703 513 Size: 552 939 165 534 4585 Fragments arranged by size: 4585 939 552 534 165 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3028 487 Size: 683 56 1802 4234 Fragments arranged by size: 4234 1802 683 56 BtsI GCAGTG_nn' Cuts at: 2082 4291 5085 5105 2082 Size: 2209 794 20 3752 Fragments arranged by size: 3752 2209 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6775 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6740 DraI TTT'AAA Cuts at: 4079 4079 Size: 6775 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5782 809 Size: 507 4466 1802 Fragments arranged by size: 4466 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2712 3258 3417 Size: 54 1425 50 275 18 882 546 159 Cuts at: 3417 3546 3651 4222 5111 6740 8 Size: 129 105 571 889 1629 43 Fragments arranged by size: 1629 1425 889 882 571 546 275 159 129 105 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4222 1537 Size: 275 2410 4090 Fragments arranged by size: 4090 2410 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2232 2527 2632 4705 1353 Size: 879 295 105 2073 3423 Fragments arranged by size: 3423 2073 879 295 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5362 5736 5882 387 Size: 1064 138 3773 374 146 1280 Fragments arranged by size: 3773 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2481 2481 Size: 6775 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2389 2467 3443 3749 3824 4838 Size: 393 581 176 78 976 306 75 1014 Cuts at: 4838 5435 6222 6490 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1014 976 787 597 581 393 306 268 176 78 75 EcoO109I rG'GnC_Cy Cuts at: 4036 4036 Size: 6775 EcoRI G'AATT_C Cuts at: 4084 4462 4084 Size: 378 6397 FspI TGC'GCA Cuts at: 5281 5281 Size: 6775 HaeII r_GCGC'y Cuts at: 1678 2367 5922 1678 Size: 689 3555 2531 Fragments arranged by size: 3555 2531 689 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2643 2675 3352 3384 5416 Size: 32 135 32 1071 32 677 32 2032 Cuts at: 5416 5448 5490 5522 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2032 1071 677 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3246 4119 4365 669 Size: 988 281 1308 873 246 3079 Fragments arranged by size: 3079 1308 988 873 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6775 HpaI GTT'AAC Cuts at: 4365 4365 Size: 6775 KpnI G_GTAC'C Cuts at: 3514 4100 3514 Size: 586 6189 MfeI C'AATT_G Cuts at: 4374 4374 Size: 6775 MluI A'CGCG_T Cuts at: 3745 6651 3745 Size: 2906 3869 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2359 2451 2969 3411 3745 5889 6073 1305 Size: 1054 92 518 442 334 2144 184 2007 Fragments arranged by size: 2144 2007 1054 518 442 334 184 92 MscI TGG'CCA Cuts at: 10 64 1489 1832 2714 3260 3548 10 Size: 54 1425 343 882 546 288 3237 Fragments arranged by size: 3237 1425 882 546 343 288 54 MslI CAynn'nnrTG Cuts at: 518 1166 2198 2558 2612 3542 4733 5092 5251 Size: 648 1032 360 54 930 1191 359 159 Cuts at: 5251 518 Size: 2042 Fragments arranged by size: 2042 1191 1032 930 648 360 359 159 54 MspA1I CmG'CkG Cuts at: 1606 2374 2836 3628 3787 3924 4191 4868 6016 Size: 768 462 792 159 137 267 677 1148 Cuts at: 6016 6261 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 792 768 677 462 267 245 159 137 NaeI GCC'GGC Cuts at: 3655 3655 Size: 6775 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5284 Fragments arranged by size: 5284 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6775 NgoMIV G'CCGG_C Cuts at: 3653 3653 Size: 6775 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6775 NotI GC'GGCC_GC Cuts at: 4222 4222 Size: 6775 NsiI A_TGCA'T Cuts at: 3445 3445 Size: 6775 NspI r_CATG'y Cuts at: 1132 1229 4133 5678 1132 Size: 97 2904 1545 2229 Fragments arranged by size: 2904 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3810 1348 Size: 2462 4313 PciI A'CATG_T Cuts at: 5674 5674 Size: 6775 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6775 PfoI T'CCnGG_A Cuts at: 3081 3081 Size: 6775 PmeI GTTT'AAAC Cuts at: 4079 4079 Size: 6775 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4844 4876 6357 6389 4844 Size: 32 1481 32 5230 Fragments arranged by size: 5230 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4036 4036 Size: 6775 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6775 PsiI TTA'TAA Cuts at: 4345 4345 Size: 6775 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3569 3601 3569 Size: 32 6743 PstI C_TGCA'G Cuts at: 830 3732 3929 4127 830 Size: 2902 197 198 3478 Fragments arranged by size: 3478 2902 198 197 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6775 PvuII CAG'CTG Cuts at: 2374 2836 2374 Size: 462 6313 SacI G_AGCT'C Cuts at: 2483 2483 Size: 6775 SalI G'TCGA_C Cuts at: 1936 4117 1936 Size: 2181 4594 SapI GCTCTTCn'nnn_ Cuts at: 2632 2632 Size: 6775 SbfI CC_TGCA'GG Cuts at: 4127 4127 Size: 6775 ScaI AGT'ACT Cuts at: 5023 6735 5023 Size: 1712 5063 SfcI C'TryA_G Cuts at: 826 1045 3728 3925 4123 5258 5939 6130 826 Size: 219 2683 197 198 1135 681 191 1471 Fragments arranged by size: 2683 1471 1135 681 219 198 197 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6775 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6775 SmaI CCC'GGG Cuts at: 1872 4102 1872 Size: 2230 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2726 3176 4879 5780 6042 820 Size: 197 926 783 450 1703 901 262 1553 Fragments arranged by size: 1703 1553 926 901 783 450 262 197 SnaBI TAC'GTA Cuts at: 493 4566 493 Size: 4073 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6775 SphI G_CATG'C Cuts at: 1132 4133 1132 Size: 3001 3774 SspI AAT'ATT Cuts at: 5 52 4699 5 Size: 47 4647 2081 Fragments arranged by size: 4647 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6775 StyI C'CwwG_G Cuts at: 513 1065 2004 2290 3549 4071 4214 513 Size: 552 939 286 1259 522 143 3074 Fragments arranged by size: 3074 1259 939 552 522 286 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2732 4073 4802 4819 4972 5157 5602 Size: 1255 1152 1341 729 17 153 185 445 Cuts at: 5602 325 Size: 1498 Fragments arranged by size: 1498 1341 1255 1152 729 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5021 6733 96 Size: 274 80 33 51 1443 3044 1712 138 Fragments arranged by size: 3044 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3330 3362 6735 6767 3330 Size: 32 3373 32 3338 Fragments arranged by size: 3373 3338 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6775 XbaI T'CTAG_A Cuts at: 4111 4111 Size: 6775 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2434 2768 3412 2434 Size: 334 644 5797 Fragments arranged by size: 5797 644 334 XhoI C'TCGA_G Cuts at: 1943 2726 1943 Size: 783 5992 XmaI C'CCGG_G Cuts at: 1870 4100 1870 Size: 2230 4545 XmnI GAAnn'nnTTC Cuts at: 4904 4904 Size: 6775 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4583 276 Size: 53 83 186 936 3049 2468 Fragments arranged by size: 3049 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI