Restriction map for
pFN21AE2419
(Linear) (Six-base) MAPSORT of: KIEE2419.seq Check: 5137 from: 1 to: 2070
KIEE2419.orf
With 182 enzymes: SgfI *
June 11, 2014 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 597 2070
Size: 597 1473
Acc65I G'GTAC_C
Cuts at: 0 1505 2070
Size: 1505 565
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 384 1819 2070
Size: 208 176 1435 251
Fragments arranged by size:
1435 251 208 176
AflIII A'CryG_T
Cuts at: 0 1740 2070
Size: 1740 330
AleI CACnn'nnGTG
Cuts at: 0 193 2070
Size: 193 1877
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 95 1117 2070
Size: 95 1022 953
Fragments arranged by size:
1022 953 95
ApoI r'AATT_y
Cuts at: 0 1065 2070
Size: 1065 1005
AvaI C'yCGr_G
Cuts at: 0 231 721 2070
Size: 231 490 1349
Fragments arranged by size:
1349 490 231
BanI G'GyrC_C
Cuts at: 0 126 1093 1390 1505 2070
Size: 126 967 297 115 565
Fragments arranged by size:
967 565 297 126 115
BanII G_rGCy'C
Cuts at: 0 188 478 1165 1381 2070
Size: 188 290 687 216 689
Fragments arranged by size:
689 687 290 216 188
BbsI GAAGACnn'nnnn_
Cuts at: 0 1207 1581 1680 2070
Size: 1207 374 99 390
Fragments arranged by size:
1207 390 374 99
BclI T'GATC_A
Cuts at: 0 1139 1598 2070
Size: 1139 459 472
Fragments arranged by size:
1139 472 459
BfrBI ATG'CAT
Cuts at: 0 1438 2070
Size: 1438 632
BglI GCCn_nnn'nGGC
Cuts at: 0 1411 2070
Size: 1411 659
BglII A'GATC_T
Cuts at: 0 783 2070
Size: 783 1287
Bme1580I G_kGCm'C
Cuts at: 0 1096 2070
Size: 1096 974
BmgBI CAC'GTC
Cuts at: 0 1310 2070
Size: 1310 760
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 1438 1744 2070
Size: 462 976 306 326
Fragments arranged by size:
976 462 326 306
Bpu10I CC'TnA_GC
Cuts at: 0 1419 2021 2070
Size: 1419 602 49
Fragments arranged by size:
1419 602 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1156 2070
Size: 1156 914
BsaI GGTCTCn'nnnn_
Cuts at: 0 94 1981 2070
Size: 94 1887 89
Fragments arranged by size:
1887 94 89
BsaBI GATnn'nnATC
Cuts at: 0 1333 2070
Size: 1333 737
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 638 668 952 982 1697 1727 2070
Size: 638 30 284 30 715 30 343
Fragments arranged by size:
715 638 343 284 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1458 1960 2070
Size: 1458 502 110
Fragments arranged by size:
1458 502 110
BseYI C'CCAG_C
Cuts at: 0 29 734 1374 1551 2070
Size: 29 705 640 177 519
Fragments arranged by size:
705 640 519 177 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 948 2070
Size: 490 458 1122
Fragments arranged by size:
1122 490 458
BsiHKAI G_wGCw'C
Cuts at: 0 340 478 692 1172 2070
Size: 340 138 214 480 898
Fragments arranged by size:
898 480 340 214 138
BsmI GAATG_Cn'
Cuts at: 0 2062 2070
Size: 2062 8
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1824 2070
Size: 1824 246
Bsp1286I G_dGCh'C
Cuts at: 0 188 340 478 692 1096 1165 1172 1381
Size: 188 152 138 214 404 69 7 209
Cuts at: 1381 2070
Size: 689
Fragments arranged by size:
689 404 214 209 188 152 138 69
7
BspHI T'CATG_A
Cuts at: 0 1142 2070
Size: 1142 928
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 55 597 1170 2070
Size: 55 542 573 900
Fragments arranged by size:
900 573 542 55
BsrDI GCAATG_nn'
Cuts at: 0 520 866 1440 2070
Size: 520 346 574 630
Fragments arranged by size:
630 574 520 346
BsrFI r'CCGG_y
Cuts at: 0 1648 1831 2070
Size: 1648 183 239
Fragments arranged by size:
1648 239 183
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1928 1931 2070
Size: 1928 3 139
Fragments arranged by size:
1928 139 3
BstEII G'GTnAC_C
Cuts at: 0 456 2070
Size: 456 1614
BstXI CCAn_nnnn'nTGG
Cuts at: 0 195 292 373 555 1539 1551 2070
Size: 195 97 81 182 984 12 519
Fragments arranged by size:
984 519 195 182 97 81 12
BstYI r'GATC_y
Cuts at: 0 783 1080 1154 2070
Size: 783 297 74 916
Fragments arranged by size:
916 783 297 74
BtgI C'CryG_G
Cuts at: 0 164 698 2070
Size: 164 534 1372
Fragments arranged by size:
1372 534 164
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1023 2070
Size: 1023 1047
BtsI GCAGTG_nn'
Cuts at: 0 77 2070
Size: 77 1993
EaeI y'GGCC_r
Cuts at: 0 707 1253 1412 1541 1646 2070
Size: 707 546 159 129 105 424
Fragments arranged by size:
707 546 424 159 129 105
EarI CTCTTCn'nnn_
Cuts at: 0 227 522 627 2070
Size: 227 295 105 1443
Fragments arranged by size:
1443 295 227 105
EcoICRI GAG'CTC
Cuts at: 0 476 2070
Size: 476 1594
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 384 462 1438 1744 1819 2070
Size: 208 176 78 976 306 75 251
Fragments arranged by size:
976 306 251 208 176 78 75
EcoO109I rG'GnC_Cy
Cuts at: 0 2031 2070
Size: 2031 39
HaeII r_GCGC'y
Cuts at: 0 362 2070
Size: 362 1708
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 638 670 1347 1379 2070
Size: 638 32 677 32 691
Fragments arranged by size:
691 677 638 32 32
HincII GTy'rAC
Cuts at: 0 1241 2070
Size: 1241 829
KpnI G_GTAC'C
Cuts at: 0 1509 2070
Size: 1509 561
MluI A'CGCG_T
Cuts at: 0 1740 2070
Size: 1740 330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 446 964 1406 1740 2070
Size: 354 92 518 442 334 330
Fragments arranged by size:
518 442 354 334 330 92
MscI TGG'CCA
Cuts at: 0 709 1255 1543 2070
Size: 709 546 288 527
Fragments arranged by size:
709 546 527 288
MslI CAynn'nnrTG
Cuts at: 0 193 553 607 1537 2070
Size: 193 360 54 930 533
Fragments arranged by size:
930 533 360 193 54
MspA1I CmG'CkG
Cuts at: 0 369 831 1623 1782 1919 2070
Size: 369 462 792 159 137 151
Fragments arranged by size:
792 462 369 159 151 137
NaeI GCC'GGC
Cuts at: 0 1650 2070
Size: 1650 420
NgoMIV G'CCGG_C
Cuts at: 0 1648 2070
Size: 1648 422
NsiI A_TGCA'T
Cuts at: 0 1440 2070
Size: 1440 630
PasI CC'CwG_GG
Cuts at: 0 1805 2070
Size: 1805 265
PfoI T'CCnGG_A
Cuts at: 0 1076 2070
Size: 1076 994
PpuMI rG'GwC_Cy
Cuts at: 0 2031 2070
Size: 2031 39
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1564 1596 2070
Size: 1564 32 474
Fragments arranged by size:
1564 474 32
PstI C_TGCA'G
Cuts at: 0 1727 1924 2070
Size: 1727 197 146
Fragments arranged by size:
1727 197 146
PvuII CAG'CTG
Cuts at: 0 369 831 2070
Size: 369 462 1239
Fragments arranged by size:
1239 462 369
SacI G_AGCT'C
Cuts at: 0 478 2070
Size: 478 1592
SapI GCTCTTCn'nnn_
Cuts at: 0 627 2070
Size: 627 1443
SfcI C'TryA_G
Cuts at: 0 1723 1920 2070
Size: 1723 197 150
Fragments arranged by size:
1723 197 150
SmlI C'TyrA_G
Cuts at: 0 721 1171 2070
Size: 721 450 899
Fragments arranged by size:
899 721 450
StyI C'CwwG_G
Cuts at: 0 285 1544 2070
Size: 285 1259 526
Fragments arranged by size:
1259 526 285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 727 2068 2070
Size: 727 1341 2
Fragments arranged by size:
1341 727 2
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1325 1357 2070
Size: 1325 32 713
Fragments arranged by size:
1325 713 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 429 763 1407 2070
Size: 429 334 644 663
Fragments arranged by size:
663 644 429 334
XhoI C'TCGA_G
Cuts at: 0 721 2070
Size: 721 1349
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI ApoI AvaI
BanI BanII BbsI BclI BfrBI BglI BglII Bme1580I
BmgBI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrDI BsrFI BstAPI BstEII BstXI BstYI BtgI BtgZI
BtsI EaeI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I
HincII KpnI MluI MmeI MscI MslI MspA1I NaeI
NgoMIV NsiI PasI PfoI PpuMI PsrI PstI PvuII
SacI SapI SfcI SmlI StyI TaqII TstI XcmI
XhoI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BlpI BmrI BmtI
BplI BsaAI BsaHI BsaWI BsiEI BsiWI BspEI BsrBI
BsrGI BssHII BssSI BstBI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EagI EciI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI MfeI
NarI NcoI NdeI NheI NotI NruI NspI PacI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XmaI XmnI ZraI