Restriction map for
pFN21AE3759
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII AccI AccIII AclI AflII
AflIII AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
AspEI AspHI AspI AviII AvrII
BalI BbrPI BbsI BbvCI BciVI
BclI BfrI BglI BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BseAI BsgI BsiHKAI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII ClaI CpoI DraII DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRV EcoT14I
FbaI FseI FspI HpaI HphI
MamI MluI MluNI MroI MscI
NcoI NdeI NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SalI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 554 GGTACC G^GTACC
AgeI 23 ACCGGT A^CCGGT
ApaI 104 GGGCCC GGGCC^C
Asp700I 291 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 554 GGTACC G^GTACC
AvaI 202 CCCGGG C^[CT]CG[GA]G
BbeI 121 GGCGCC GGCGC^C
BcgI 564 CGAAGCGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 216 GGTCTC GGTCTC(1/5)
BsiEI 21 CGACCG CG[GA][CT]^CG
BsmBI 1298 GAGACG CGTCTC(1/5)
BsmFI 206 GGGAC GGGAC(10/14)
BsoBI 202 CCCGGG C^[CT]CG[GA]G
BsrBI 73 GAGCGG CCGCTC(-3/-3)
BsrDI 917 GCAATG GCAATG(2/0)
BsrI 1088 ACTGG ACTGG(1/-1)
BstXI 1081 CCATTTCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1051 TTTAAA TTT^AAA
DrdI 208 GACGTGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 525 TGGCCG [CT]^GGCC[GA]
EcoNI 1281 CCTTACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1003 GAATTC G^AATTC
EcoT22I 293 ATGCAT ATGCA^T
HincII 467 GTCAAC GT[CT]^[GA]AC
HindII 467 GTCAAC GT[CT]^[GA]AC
HindIII 592 AAGCTT A^AGCTT
KasI 121 GGCGCC G^GCGCC
KpnI 554 GGTACC GGTAC^C
MaeII 209 ACGT A^CGT
MslI 1057 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 175 GCCGGC GCC^GGC
NarI 121 GGCGCC GG^CGCC
NgoMIV 175 GCCGGC G^CCGGC
NlaIII 1164 CATG CATG^
NsiI 293 ATGCAT ATGCA^T
PinAI 23 ACCGGT A^CCGGT
SapI 454 GAAGAGC GCTCTTC(1/4)
SfoI 121 GGCGCC GGC^GCC
SmaI 202 CCCGGG CCC^GGG
Tsp45I 861 GTCAC ^GT[GC]AC
XmaCI 202 CCCGGG C^CCGGG
XmaI 202 CCCGGG C^CCGGG
XmnI 291 GAATGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 30 GGCGTC G[GA]^CG[CT]C
121 GGCGCC
AvaII 243 GGACC G^G[AT]CC
485 GGACC
BamHI 6 GGATCC G^GATCC
411 GGATCC
BfaI 1039 CTAG C^TAG
1321 CTAG
BglII 1191 AGATCT A^GATCT
1271 AGATCT
BsaHI 30 GGCGTC G[GA]^CG[CT]C
121 GGCGCC
BsaWI 23 ACCGGT [AT]^CCGG[AT]
245 ACCGGA
BsmI 291 GAATGC GAATGC(1/-1)
295 GCATTC
BsrFI 23 ACCGGT [GA]^CCGG[CT]
175 GCCGGC
CfoI 122 GCGC GCG^C
134 GCGC
Cfr10I 23 ACCGGT [GA]^CCGG[CT]
175 GCCGGC
EarI 454 GAAGAG CTCTTC(1/4)
1305 GAAGAG
HaeII 121 GGCGCC [GA]GCGC^[CT]
133 AGCGCC
HgaI 151 GACGC GACGC(5/10)
31 GCGTC
HhaI 122 GCGC GCG^C
134 GCGC
Hin1I 30 GGCGTC G[GA]^CG[CT]C
121 GGCGCC
HinP1I 122 GCGC G^CGC
134 GCGC
Ksp632I 454 GAAGAG CTCTTC(1/4)
1305 GAAGAG
MaeI 1039 CTAG C^TAG
1321 CTAG
MfeI 1025 CAATTG C^AATTG
1413 CAATTG
MunI 1025 CAATTG C^AATTG
1413 CAATTG
PleI 743 GACTC GAGTC(4/5)
1308 GAGTC
SmlI 380 CTCAAG C^T[CT][GA]AG
1103 CTCAAG
SspI 800 AATATT AAT^ATT
841 AATATT
TspRI 639 CAGTG CA[GC]TG[ACGT][ACGT]^
1087 CACTG
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# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI BanI BanII BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
BstNI BstUI BstYI Cac8I Cfr13I
DdeI DpnII DsaI EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI KspI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIV RsaI SacII Sau3AI
Sau96I ScrFI TfiI Tru9I TseI
Tsp509I XhoII