(Circular) (Six-base) MAPSORT of: pFN21AE3759.seq Check: 8968 from: 1 to: 6148 pFN21AE3759 6148 bp With 182 enzymes: SgfI * March 28, 2012 12:06 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3958 278 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3491 1937 Size: 1554 4594 Acc65I G'GTAC_C Cuts at: 2559 3469 2559 Size: 910 5238 AclI AA'CG_TT Cuts at: 1563 4275 4648 1563 Size: 2712 373 3063 Fragments arranged by size: 3063 2712 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2271 3150 4211 5595 5863 1632 Size: 639 879 1061 1384 268 1917 Fragments arranged by size: 1917 1384 1061 879 639 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5951 AflIII A'CryG_T Cuts at: 1203 5047 6024 1203 Size: 3844 977 1327 Fragments arranged by size: 3844 1327 977 AgeI A'CCGG_T Cuts at: 2028 4979 2028 Size: 2951 3197 AhdI GACnn_n'nnGTC Cuts at: 4877 4877 Size: 6148 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6148 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6148 AlwNI CAG_nnn'CTG Cuts at: 3891 5463 3891 Size: 1572 4576 ApaI G_GGCC'C Cuts at: 2113 2113 Size: 6148 ApaLI G'TGCA_C Cuts at: 4205 5361 4205 Size: 1156 4992 ApoI r'AATT_y Cuts at: 1493 2318 2364 2819 3008 3142 3457 3683 3835 Size: 825 46 455 189 134 315 226 152 Cuts at: 3835 5944 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 825 455 315 226 189 152 134 46 AseI AT'TA_AT Cuts at: 160 1032 4702 160 Size: 872 3670 1606 Fragments arranged by size: 3670 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 AvaI C'yCGr_G Cuts at: 1870 1943 2207 3473 1870 Size: 73 264 1266 4545 Fragments arranged by size: 4545 1266 264 73 BamHI G'GATC_C Cuts at: 2011 2416 3478 3845 2011 Size: 405 1062 367 4314 Fragments arranged by size: 4314 1062 405 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2126 2559 2576 3469 4924 Size: 325 217 627 339 433 17 893 1455 Cuts at: 4924 618 Size: 1842 Fragments arranged by size: 1842 1455 893 627 433 339 325 217 17 BanII G_rGCy'C Cuts at: 1391 2025 2057 2113 3467 1391 Size: 634 32 56 1354 4072 Fragments arranged by size: 4072 1354 634 56 32 BbeI G_GCGC'C Cuts at: 2130 2130 Size: 6148 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5177 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2558 2592 4339 4373 1903 Size: 34 621 34 1747 34 3678 Fragments arranged by size: 3678 1747 621 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4040 5250 4040 Size: 1210 4938 BclI T'GATC_A Cuts at: 1548 1548 Size: 6148 BfrBI ATG'CAT Cuts at: 2300 2300 Size: 6148 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4759 136 Size: 107 122 71 631 605 3087 1525 Fragments arranged by size: 3087 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3196 3276 6143 3196 Size: 80 2867 3201 Fragments arranged by size: 3201 2867 80 BlpI GC'TnA_GC Cuts at: 3565 3565 Size: 6148 Bme1580I G_kGCm'C Cuts at: 1790 2113 4209 5365 1790 Size: 323 2096 1156 2573 Fragments arranged by size: 2573 2096 1156 323 BmgBI CAC'GTC Cuts at: 1318 2215 1318 Size: 897 5251 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3103 3879 4837 Size: 416 53 83 399 24 1686 776 958 Cuts at: 4837 442 Size: 1753 Fragments arranged by size: 1753 1686 958 776 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6148 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2032 2032 Size: 6148 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4808 1239 Size: 3569 2579 Bpu10I CC'TnA_GC Cuts at: 2813 3392 2813 Size: 579 5569 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2370 3093 4273 5138 5436 2370 Size: 723 1180 865 298 3082 Fragments arranged by size: 3082 1180 865 723 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2227 4811 882 Size: 617 728 2584 2219 Fragments arranged by size: 2584 2219 728 617 BsaAI yAC'GTr Cuts at: 493 1204 3939 493 Size: 711 2735 2702 Fragments arranged by size: 2735 2702 711 BsaBI GATnn'nnATC Cuts at: 6142 6142 Size: 6148 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2036 2127 3955 4337 Size: 53 83 186 936 503 91 1828 382 Cuts at: 4337 4972 275 Size: 635 1451 Fragments arranged by size: 1828 1451 936 635 503 382 186 91 83 53 BsaWI w'CCGG_w Cuts at: 2028 2250 4581 4979 5253 5400 5811 2028 Size: 222 2331 398 274 147 411 2365 Fragments arranged by size: 2365 2331 411 398 274 222 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5923 Fragments arranged by size: 5923 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2078 2081 2183 2204 1186 Size: 889 3 3 102 21 5130 Fragments arranged by size: 5130 889 102 21 3 3 BseYI C'CCAG_C Cuts at: 5351 5847 5351 Size: 496 5652 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5575 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2029 3598 4359 5387 1540 Size: 275 186 28 1569 761 1028 2301 Fragments arranged by size: 2301 1569 1028 761 275 186 28 BsiHKAI G_wGCw'C Cuts at: 4209 4294 5365 4209 Size: 85 1071 4992 Fragments arranged by size: 4992 1071 85 BsmI GAATG_Cn' Cuts at: 2300 2302 3659 3752 2300 Size: 2 1357 93 4696 Fragments arranged by size: 4696 1357 93 2 BsmBI CGTCTCn'nnnn_ Cuts at: 3297 3297 Size: 6148 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2057 2113 3467 4209 4294 5365 Size: 399 235 32 56 1354 742 85 1071 Cuts at: 5365 1391 Size: 2174 Fragments arranged by size: 2174 1354 1071 742 399 235 85 56 32 BspEI T'CCGG_A Cuts at: 5811 5811 Size: 6148 BspHI T'CATG_A Cuts at: 4037 5767 4037 Size: 1730 4418 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3503 844 Size: 2659 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2080 4035 1394 Size: 429 257 1955 3507 Fragments arranged by size: 3507 1955 429 257 BsrDI GCAATG_nn' Cuts at: 65 1248 2929 4643 4817 65 Size: 1183 1681 1714 174 1396 Fragments arranged by size: 1714 1681 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2028 2180 4792 4979 1700 Size: 110 218 152 2612 187 2869 Fragments arranged by size: 2869 2612 218 187 152 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6148 BssSI C'ACGA_G Cuts at: 4208 5220 4208 Size: 1012 5136 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6148 BstBI TT'CG_AA Cuts at: 4952 4952 Size: 6148 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6148 BstXI CCAn_nnnn'nTGG Cuts at: 3093 3093 Size: 6148 BstYI r'GATC_y Cuts at: 1972 2011 2416 3196 3276 3478 3845 4230 4247 Size: 39 405 780 80 202 367 385 17 Cuts at: 4247 5699 5785 5797 6143 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 780 405 385 367 346 202 86 80 39 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2040 2096 2117 2262 513 Size: 552 939 36 56 21 145 4399 Fragments arranged by size: 4399 939 552 145 56 36 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5409 Fragments arranged by size: 5409 683 56 BtsI GCAGTG_nn' Cuts at: 3664 4458 4478 3664 Size: 794 20 5334 Fragments arranged by size: 5334 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6148 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6113 DraI TTT'AAA Cuts at: 3058 3452 3058 Size: 394 5754 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2219 5155 809 Size: 507 903 2936 1802 Fragments arranged by size: 2936 1802 903 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2530 3595 4484 Size: 54 1425 50 275 18 700 1065 889 Cuts at: 4484 6113 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1065 889 700 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3595 1537 Size: 275 1783 4090 Fragments arranged by size: 4090 1783 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2454 3305 4078 1353 Size: 1101 851 773 3423 Fragments arranged by size: 3423 1101 851 773 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4735 5109 5255 387 Size: 1064 138 3146 374 146 1280 Fragments arranged by size: 3146 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2271 3150 4211 4808 5595 5863 1239 Size: 393 639 879 1061 597 787 268 1524 Fragments arranged by size: 1524 1061 879 787 639 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3290 3290 Size: 6148 EcoRI G'AATT_C Cuts at: 3008 3457 3835 3008 Size: 449 378 5321 Fragments arranged by size: 5321 449 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3029 3029 Size: 6148 FspI TGC'GCA Cuts at: 4654 4654 Size: 6148 HaeII r_GCGC'y Cuts at: 1678 2130 2142 5295 1678 Size: 452 12 3153 2531 Fragments arranged by size: 3153 2531 452 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2536 2568 3067 3099 3256 Size: 32 135 32 964 32 499 32 157 Cuts at: 3256 3288 4789 4821 4863 4895 1373 Size: 32 1501 32 42 32 2626 Fragments arranged by size: 2626 1501 964 499 157 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2474 3492 3738 669 Size: 988 281 536 1018 246 3079 Fragments arranged by size: 3079 1018 988 536 281 246 HindIII A'AGCT_T Cuts at: 748 2597 748 Size: 1849 4299 HpaI GTT'AAC Cuts at: 3738 3738 Size: 6148 KasI G'GCGC_C Cuts at: 2126 2126 Size: 6148 KpnI G_GTAC'C Cuts at: 2563 3473 2563 Size: 910 5238 MfeI C'AATT_G Cuts at: 3030 3418 3747 3030 Size: 388 329 5431 Fragments arranged by size: 5431 388 329 MluI A'CGCG_T Cuts at: 6024 6024 Size: 6148 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2341 3359 5262 5446 1305 Size: 1036 1018 1903 184 2007 Fragments arranged by size: 2007 1903 1036 1018 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4326 Fragments arranged by size: 4326 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3066 4106 4465 4624 518 Size: 648 1900 1040 359 159 2042 Fragments arranged by size: 2042 1900 1040 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2042 2089 2098 2119 2264 3564 4241 5389 Size: 436 47 9 21 145 1300 677 1148 Cuts at: 5389 5634 1606 Size: 245 2120 Fragments arranged by size: 2120 1300 1148 677 436 245 145 47 21 9 NaeI GCC'GGC Cuts at: 2182 2182 Size: 6148 NarI GG'CG_CC Cuts at: 2127 2127 Size: 6148 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4657 Fragments arranged by size: 4657 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6148 NgoMIV G'CCGG_C Cuts at: 2180 2180 Size: 6148 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6148 NotI GC'GGCC_GC Cuts at: 3595 3595 Size: 6148 NsiI A_TGCA'T Cuts at: 2302 2302 Size: 6148 NspI r_CATG'y Cuts at: 1132 1229 3506 5051 1132 Size: 97 2277 1545 2229 Fragments arranged by size: 2277 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6148 PciI A'CATG_T Cuts at: 5047 5047 Size: 6148 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6148 PfoI T'CCnGG_A Cuts at: 2225 2225 Size: 6148 PmeI GTTT'AAAC Cuts at: 3452 3452 Size: 6148 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4217 4249 5730 5762 4217 Size: 32 1481 32 4603 Fragments arranged by size: 4603 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6148 PsiI TTA'TAA Cuts at: 3718 3718 Size: 6148 PspOMI G'GGCC_C Cuts at: 2109 2109 Size: 6148 PstI C_TGCA'G Cuts at: 830 3500 830 Size: 2670 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6148 SacII CC_GC'GG Cuts at: 2043 2099 2120 2265 2043 Size: 56 21 145 5926 Fragments arranged by size: 5926 145 56 21 SalI G'TCGA_C Cuts at: 1936 3490 1936 Size: 1554 4594 SapI GCTCTTCn'nnn_ Cuts at: 2454 2454 Size: 6148 SbfI CC_TGCA'GG Cuts at: 3500 3500 Size: 6148 ScaI AGT'ACT Cuts at: 4396 6108 4396 Size: 1712 4436 SfcI C'TryA_G Cuts at: 826 1045 3496 4631 5312 5503 826 Size: 219 2451 1135 681 191 1471 Fragments arranged by size: 2451 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2128 2128 Size: 6148 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6148 SmaI CCC'GGG Cuts at: 1872 2209 3475 1872 Size: 337 1266 4545 Fragments arranged by size: 4545 1266 337 SmlI C'TyrA_G Cuts at: 820 1017 1943 2385 3108 4252 5153 5415 820 Size: 197 926 442 723 1144 901 262 1553 Fragments arranged by size: 1553 1144 926 901 723 442 262 197 SnaBI TAC'GTA Cuts at: 493 3939 493 Size: 3446 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6148 SphI G_CATG'C Cuts at: 1132 3506 1132 Size: 2374 3774 SspI AAT'ATT Cuts at: 5 52 2807 2848 4072 5 Size: 47 2755 41 1224 2081 Fragments arranged by size: 2755 2081 1224 47 41 StuI AGG'CCT Cuts at: 1517 1517 Size: 6148 StyI C'CwwG_G Cuts at: 513 1065 2004 3587 513 Size: 552 939 1583 3074 Fragments arranged by size: 3074 1583 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4175 4192 4345 4530 4975 325 Size: 1255 2595 17 153 185 445 1498 Fragments arranged by size: 2595 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3328 4394 6106 Size: 274 80 33 51 1443 1351 1066 1712 Cuts at: 6106 96 Size: 138 Fragments arranged by size: 1712 1443 1351 1066 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6108 6140 6108 Size: 32 6116 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6148 XbaI T'CTAG_A Cuts at: 3484 3484 Size: 6148 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6148 XmaI C'CCGG_G Cuts at: 1870 2207 3473 1870 Size: 337 1266 4545 Fragments arranged by size: 4545 1266 337 XmnI GAAnn'nnTTC Cuts at: 2300 4277 2300 Size: 1977 4171 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3956 276 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SrfI SwaI XcmI