(Linear) (Six-base) MAPSORT of: KIEE3759.seq Check: 7786 from: 1 to: 1443 KIEE3759.orf With 182 enzymes: SgfI * March 22, 2012 16:43 .. Acc65I G'GTAC_C Cuts at: 0 554 1443 Size: 554 889 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 266 1145 1443 Size: 266 879 298 Fragments arranged by size: 879 298 266 AgeI A'CCGG_T Cuts at: 0 23 1443 Size: 23 1420 ApaI G_GGCC'C Cuts at: 0 108 1443 Size: 108 1335 ApoI r'AATT_y Cuts at: 0 313 359 814 1003 1137 1443 Size: 313 46 455 189 134 306 Fragments arranged by size: 455 313 306 189 134 46 AvaI C'yCGr_G Cuts at: 0 202 1443 Size: 202 1241 BamHI G'GATC_C Cuts at: 0 6 411 1443 Size: 6 405 1032 Fragments arranged by size: 1032 405 6 BanI G'GyrC_C Cuts at: 0 121 554 571 1443 Size: 121 433 17 872 Fragments arranged by size: 872 433 121 17 BanII G_rGCy'C Cuts at: 0 20 52 108 1443 Size: 20 32 56 1335 Fragments arranged by size: 1335 56 32 20 BbeI G_GCGC'C Cuts at: 0 125 1443 Size: 125 1318 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 553 587 1443 Size: 553 34 856 Fragments arranged by size: 856 553 34 BfrBI ATG'CAT Cuts at: 0 295 1443 Size: 295 1148 BglII A'GATC_T Cuts at: 0 1191 1271 1443 Size: 1191 80 172 Fragments arranged by size: 1191 172 80 Bme1580I G_kGCm'C Cuts at: 0 108 1443 Size: 108 1335 BmgBI CAC'GTC Cuts at: 0 210 1443 Size: 210 1233 BmrI ACTGGGnnnn_n' Cuts at: 0 1098 1443 Size: 1098 345 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 27 1443 Size: 27 1416 Bpu10I CC'TnA_GC Cuts at: 0 808 1387 1443 Size: 808 579 56 Fragments arranged by size: 808 579 56 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 365 1088 1443 Size: 365 723 355 Fragments arranged by size: 723 365 355 BsaI GGTCTCn'nnnn_ Cuts at: 0 222 1443 Size: 222 1221 BsaHI Gr'CG_yC Cuts at: 0 31 122 1443 Size: 31 91 1321 Fragments arranged by size: 1321 91 31 BsaWI w'CCGG_w Cuts at: 0 23 245 1443 Size: 23 222 1198 Fragments arranged by size: 1198 222 23 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 70 73 76 178 199 1443 Size: 70 3 3 102 21 1244 Fragments arranged by size: 1244 102 70 21 3 3 BsiEI CG_ry'CG Cuts at: 0 24 1443 Size: 24 1419 BsmI GAATG_Cn' Cuts at: 0 295 297 1443 Size: 295 2 1146 Fragments arranged by size: 1146 295 2 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1292 1443 Size: 1292 151 Bsp1286I G_dGCh'C Cuts at: 0 20 52 108 1443 Size: 20 32 56 1335 Fragments arranged by size: 1335 56 32 20 BsrBI CCG'CTC Cuts at: 0 75 1443 Size: 75 1368 BsrDI GCAATG_nn' Cuts at: 0 924 1443 Size: 924 519 BsrFI r'CCGG_y Cuts at: 0 23 175 1443 Size: 23 152 1268 Fragments arranged by size: 1268 152 23 BstXI CCAn_nnnn'nTGG Cuts at: 0 1088 1443 Size: 1088 355 BstYI r'GATC_y Cuts at: 0 6 411 1191 1271 1443 Size: 6 405 780 80 172 Fragments arranged by size: 780 405 172 80 6 BtgI C'CryG_G Cuts at: 0 35 91 112 257 1443 Size: 35 56 21 145 1186 Fragments arranged by size: 1186 145 56 35 21 DraI TTT'AAA Cuts at: 0 1053 1443 Size: 1053 390 DrdI GACnn_nn'nnGTC Cuts at: 0 214 1443 Size: 214 1229 EaeI y'GGCC_r Cuts at: 0 525 1443 Size: 525 918 EarI CTCTTCn'nnn_ Cuts at: 0 449 1300 1443 Size: 449 851 143 Fragments arranged by size: 851 449 143 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 266 1145 1443 Size: 266 879 298 Fragments arranged by size: 879 298 266 EcoNI CCTnn'n_nnAGG Cuts at: 0 1285 1443 Size: 1285 158 EcoRI G'AATT_C Cuts at: 0 1003 1443 Size: 1003 440 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1024 1443 Size: 1024 419 HaeII r_GCGC'y Cuts at: 0 125 137 1443 Size: 125 12 1306 Fragments arranged by size: 1306 125 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 531 563 1062 1094 1251 1283 1443 Size: 531 32 499 32 157 32 160 Fragments arranged by size: 531 499 160 157 32 32 32 HincII GTy'rAC Cuts at: 0 469 1443 Size: 469 974 HindIII A'AGCT_T Cuts at: 0 592 1443 Size: 592 851 KasI G'GCGC_C Cuts at: 0 121 1443 Size: 121 1322 KpnI G_GTAC'C Cuts at: 0 558 1443 Size: 558 885 MfeI C'AATT_G Cuts at: 0 1025 1413 1443 Size: 1025 388 30 Fragments arranged by size: 1025 388 30 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 336 1354 1443 Size: 336 1018 89 Fragments arranged by size: 1018 336 89 MslI CAynn'nnrTG Cuts at: 0 1061 1443 Size: 1061 382 MspA1I CmG'CkG Cuts at: 0 37 84 93 114 259 1443 Size: 37 47 9 21 145 1184 Fragments arranged by size: 1184 145 47 37 21 9 NaeI GCC'GGC Cuts at: 0 177 1443 Size: 177 1266 NarI GG'CG_CC Cuts at: 0 122 1443 Size: 122 1321 NgoMIV G'CCGG_C Cuts at: 0 175 1443 Size: 175 1268 NsiI A_TGCA'T Cuts at: 0 297 1443 Size: 297 1146 PfoI T'CCnGG_A Cuts at: 0 220 1443 Size: 220 1223 PspOMI G'GGCC_C Cuts at: 0 104 1443 Size: 104 1339 SacII CC_GC'GG Cuts at: 0 38 94 115 260 1443 Size: 38 56 21 145 1183 Fragments arranged by size: 1183 145 56 38 21 SapI GCTCTTCn'nnn_ Cuts at: 0 449 1443 Size: 449 994 SfoI GGC'GCC Cuts at: 0 123 1443 Size: 123 1320 SmaI CCC'GGG Cuts at: 0 204 1443 Size: 204 1239 SmlI C'TyrA_G Cuts at: 0 380 1103 1443 Size: 380 723 340 Fragments arranged by size: 723 380 340 SspI AAT'ATT Cuts at: 0 802 843 1443 Size: 802 41 600 Fragments arranged by size: 802 600 41 TatI w'GTAC_w Cuts at: 0 1323 1443 Size: 1323 120 XmaI C'CCGG_G Cuts at: 0 202 1443 Size: 202 1241 XmnI GAAnn'nnTTC Cuts at: 0 295 1443 Size: 295 1148 Enzymes that do cut: Acc65I AcuI AgeI ApaI ApoI AvaI BamHI BanI BanII BbeI BcgI BfrBI BglII Bme1580I BmgBI BmrI BplI Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BsiEI BsmI BsmBI Bsp1286I BsrBI BsrDI BsrFI BstXI BstYI BtgI DraI DrdI EaeI EarI Eco57MI EcoNI EcoRI FalI HaeII Hin4I HincII HindIII KasI KpnI MfeI MmeI MslI MspA1I NaeI NarI NgoMIV NsiI PfoI PspOMI SacII SapI SfoI SmaI SmlI SspI TatI XmaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AhdI AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BbsI BbvCI BciVI BclI BglI BlpI BmtI BpmI BsaAI BsaBI BsaXI BseYI BsgI BsiHKAI BsiWI BspEI BspHI BspMI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII EagI EciI EcoICRI EcoO109I EcoRV FseI FspI FspAI HpaI MluI MscI NcoI NdeI NheI NotI NruI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI StuI StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI ZraI