################################## # NO CUT ENZYMES ( 178 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspI AvaI AviII AvrII BalI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CfoI ClaI CpoI DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FokI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HphI ItaI KasI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MunI MvnI MwoI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TseI Tsp45I Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AfaI 572 GTAC GT^AC AhdI 25 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AluI 452 AGCT AG^CT Asp700I 322 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 25 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 428 GAGCAC G[AT]GC[AT]^C AvaII 4 GGTCC G^G[AT]CC BamHI 243 GGATCC G^GATCC BanI 126 GGTGCC G^G[CT][GA]CC BlpI 453 GCTAAGC GC^T[ACGT]AGC Bpu1102I 453 GCTAAGC GC^T[ACGT]AGC BsiHKAI 428 GAGCAC G[AT]GC[AT]^C BspMI 162 GCAGGT ACCTGC(4/8) BsrFI 395 GCCGGC [GA]^CCGG[CT] CelII 453 GCTAAGC GC^T[ACGT]AGC Cfr10I 395 GCCGGC [GA]^CCGG[CT] DraII 3 GGGTCCT [GA]G^G[ACGT]CC[CT] Eam1105I 25 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 3 GGGTCCT [GA]G^G[ACGT]CC[CT] HapII 396 CCGG C^CGG HincII 622 GTTAAC GT[CT]^[GA]AC HindII 622 GTTAAC GT[CT]^[GA]AC HpaI 622 GTTAAC GTT^AAC HpaII 396 CCGG C^CGG MaeIII 68 GTTAC ^GT[ACGT]AC MspI 396 CCGG C^CGG NaeI 395 GCCGGC GCC^GGC NgoMIV 395 GCCGGC G^CCGGC PflMI 188 CCACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 3 GGGTCCT [GA]G^G[AT]CC[CT] RsaI 572 GTAC GT^AC SfaNI 640 GCATC GCATC(5/9) SmlI 145 CTTGAG C^T[CT][GA]AG TaqI 270 TCGA T^CGA TthHB8I 270 TCGA T^CGA Van91I 188 CCACAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 403 TCTAGA T^CTAGA XcmI 325 CCAATTCCTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 322 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### BfaI 8 CTAG C^TAG 404 CTAG BmyI 127 GTGCCC G[AGT]GC[ACT]^C 428 GAGCAC BsiYI 188 CCACAACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 312 CCCCAAACTGG BslI 188 CCACAACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 312 CCCCAAACTGG Bsp1286I 127 GTGCCC G[AGT]GC[ACT]^C 428 GAGCAC BsrI 318 ACTGG ACTGG(1/-1) 256 CCAGT BssKI 158 CCTGG ^CC[ACGT]GG 218 CCTGG BstNI 158 CCTGG CC^[AT]GG 218 CCTGG BstYI 243 GGATCC [GA]^GATC[CT] 503 AGATCC Cac8I 395 GCCGGC GC[ACGT]^[ACGT]GC 539 GCTGGC Cfr13I 4 GGTCC G^G[ACGT]CC 338 GGCCC DdeI 283 CTAAG C^T[ACGT]AG 454 CTAAG DraI 177 TTTAAA TTT^AAA 204 TTTAAA EarI 91 GAAGAG CTCTTC(1/4) 112 GAAGAG EcoRII 158 CCTGG ^CC[AT]GG 218 CCTGG HaeIII 338 GGCC GG^CC 348 GGCC Ksp632I 91 GAAGAG CTCTTC(1/4) 112 GAAGAG MaeI 8 CTAG C^TAG 404 CTAG MflI 243 GGATCC [GA]^GATC[CT] 503 AGATCC MvaI 158 CCTGG CC^[AT]GG 218 CCTGG Sau96I 4 GGTCC G^G[ACGT]CC 338 GGCCC ScrFI 158 CCTGG CC^[ACGT]GG 218 CCTGG SspI 61 AATATT AAT^ATT 182 AATATT TfiI 37 GAATC G^A[AT]TC 100 GATTC XhoII 243 GGATCC [GA]^GATC[CT] 503 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AlwI BsmFI DpnII HinfI MboI MboII MnlI MseI NdeII NlaIII NlaIV PleI Sau3AI Tru9I Tsp509I TspRI