################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV FbaI FseI FspI HapII HgaI Hin1I HindIII HpaII KasI KpnI Ksp632I KspI MaeI MaeIII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AccI 431 GTCGAC GT^[AC][GT]AC AcsI 629 AAATTC [GA]^AATT[CT] ApoI 629 AAATTC [GA]^AATT[CT] AseI 778 ATTAAT AT^TAAT AsnI 778 ATTAAT AT^TAAT AvaI 178 CCCGAG C^[CT]CG[GA]G BamHI 240 GGATCC G^GATCC BanI 112 GGCACC G^G[CT][GA]CC BbsI 481 GAAGAC GAAGAC(2/6) BglII 668 AGATCT A^GATCT BmyI 111 GGGCAC G[AGT]GC[ACT]^C BpuAI 481 GAAGAC GAAGAC(2/6) BsgI 198 CTGCAC GTGCAG(16/14) BsoBI 178 CCCGAG C^[CT]CG[GA]G Bsp1286I 111 GGGCAC G[AGT]GC[ACT]^C BstAPI 48 GCAGCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraI 220 TTTAAA TTT^AAA DraII 693 GGGACCC [GA]G^G[ACGT]CC[CT] EcoNI 639 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 693 GGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 539 CCAAGG C^C[AT][AT]GG EcoT22I 679 ATGCAT ATGCA^T HaeII 228 GGCGCT [GA]GCGC^[CT] HpaI 346 GTTAAC GTT^AAC HphI 555 GGTGA GGTGA(8/7) MaeII 563 ACGT A^CGT NsiI 679 ATGCAT ATGCA^T PpuMI 693 GGGACCC [GA]G^G[AT]CC[CT] PshBI 778 ATTAAT AT^TAAT PstI 46 CTGCAG CTGCA^G SalI 431 GTCGAC G^TCGAC SfcI 46 CTGCAG C^T[GA][CT]AG SmlI 420 CTTGAG C^T[CT][GA]AG StyI 539 CCAAGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AfaI 159 GTAC GT^AC 768 GTAC AlwNI 37 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG 49 CAGCTTCTG AvaII 694 GGACC G^G[AT]CC 816 GGACC BpmI 34 CTCCAG CTGGAG(16/14) 640 CTCCAG BstYI 240 GGATCC [GA]^GATC[CT] 668 AGATCT HaeIII 6 GGCC GG^CC 145 GGCC HincII 346 GTTAAC GT[CT]^[GA]AC 431 GTCGAC HindII 346 GTTAAC GT[CT]^[GA]AC 431 GTCGAC MboII 481 GAAGA GAAGA(8/7) 137 TCTTC MflI 240 GGATCC [GA]^GATC[CT] 668 AGATCT PvuII 37 CAGCTG CAG^CTG 771 CAGCTG RsaI 159 GTAC GT^AC 768 GTAC SfaNI 22 GCATC GCATC(5/9) 523 GCATC XhoII 240 GGATCC [GA]^GATC[CT] 668 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AccII AciI AluI AlwI BbvI BsaJI BsiYI BslI BsmFI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EcoRII FauI Fnu4HI FokI HhaI HinP1I HinfI ItaI MboI MnlI MseI MspA1I MvaI MvnI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I