(Circular) (Six-base) MAPSORT of: pFN21AE1804.seq Check: 5402 from: 1 to: 5659 pFN21AE1804 5659 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3469 278 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2437 3002 1937 Size: 500 565 4594 Fragments arranged by size: 4594 565 500 Acc65I G'GTAC_C Cuts at: 2980 2980 Size: 5659 AclI AA'CG_TT Cuts at: 1563 3786 4159 1563 Size: 2223 373 3063 Fragments arranged by size: 3063 2223 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2774 3722 5106 5374 1632 Size: 1142 948 1384 268 1917 Fragments arranged by size: 1917 1384 1142 948 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5462 AflIII A'CryG_T Cuts at: 1203 4558 5535 1203 Size: 3355 977 1327 Fragments arranged by size: 3355 1327 977 AgeI A'CCGG_T Cuts at: 4490 4490 Size: 5659 AhdI GACnn_n'nnGTC Cuts at: 4388 4388 Size: 5659 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5659 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5659 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2750 2782 2750 Size: 32 5627 AlwNI CAG_nnn'CTG Cuts at: 2047 2059 3402 4974 2047 Size: 12 1343 1572 2732 Fragments arranged by size: 2732 1572 1343 12 ApaLI G'TGCA_C Cuts at: 3716 4872 3716 Size: 1156 4503 ApoI r'AATT_y Cuts at: 1493 2634 2968 3194 3346 5455 1493 Size: 1141 334 226 152 2109 1697 Fragments arranged by size: 2109 1697 1141 334 226 152 AseI AT'TA_AT Cuts at: 160 1032 2784 4213 160 Size: 872 1752 1429 1606 Fragments arranged by size: 1752 1606 1429 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 AvaI C'yCGr_G Cuts at: 1870 1943 2183 2984 1870 Size: 73 240 801 4545 Fragments arranged by size: 4545 801 240 73 BamHI G'GATC_C Cuts at: 2245 2989 3356 2245 Size: 744 367 4548 Fragments arranged by size: 4548 744 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2117 2980 4435 618 Size: 325 217 627 330 863 1455 1842 Fragments arranged by size: 1842 1455 863 627 330 325 217 BanII G_rGCy'C Cuts at: 1391 2978 1391 Size: 1587 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2493 928 Size: 971 594 4094 Fragments arranged by size: 4094 971 594 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3850 3884 1903 Size: 34 1913 34 3678 Fragments arranged by size: 3678 1913 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3551 4761 3551 Size: 1210 4449 BclI T'GATC_A Cuts at: 1548 1548 Size: 5659 BfrBI ATG'CAT Cuts at: 2686 2686 Size: 5659 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4270 136 Size: 107 122 71 631 605 337 2261 1525 Fragments arranged by size: 2261 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2673 5654 2673 Size: 2981 2678 BlpI GC'TnA_GC Cuts at: 3076 3076 Size: 5659 Bme1580I G_kGCm'C Cuts at: 1790 2120 3720 4876 1790 Size: 330 1600 1156 2573 Fragments arranged by size: 2573 1600 1156 330 BmgBI CAC'GTC Cuts at: 1318 2569 1318 Size: 1251 4408 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3390 4348 442 Size: 416 53 83 399 24 1973 958 1753 Fragments arranged by size: 1973 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5659 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2024 2630 4319 1239 Size: 785 606 1689 2579 Fragments arranged by size: 2579 1689 785 606 Bpu10I CC'TnA_GC Cuts at: 2390 2390 Size: 5659 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2446 3784 4649 4947 2446 Size: 1338 865 298 3158 Fragments arranged by size: 3158 1338 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4322 882 Size: 617 2823 2219 Fragments arranged by size: 2823 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3450 493 Size: 711 2246 2702 Fragments arranged by size: 2702 2246 711 BsaBI GATnn'nnATC Cuts at: 5653 5653 Size: 5659 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3466 3848 4483 275 Size: 53 83 186 936 1933 382 635 1451 Fragments arranged by size: 1933 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4092 4490 4764 4911 5322 4092 Size: 398 274 147 411 4429 Fragments arranged by size: 4429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5434 Fragments arranged by size: 5434 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5659 BseYI C'CCAG_C Cuts at: 4862 5358 4862 Size: 496 5163 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2188 1163 Size: 573 452 4634 Fragments arranged by size: 4634 573 452 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3109 3870 4898 1540 Size: 275 186 1108 761 1028 2301 Fragments arranged by size: 2301 1108 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3720 3805 4876 3720 Size: 85 1071 4503 Fragments arranged by size: 4503 1071 85 BsmI GAATG_Cn' Cuts at: 3170 3263 3170 Size: 93 5566 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2120 2978 3720 3805 4876 1391 Size: 399 330 858 742 85 1071 2174 Fragments arranged by size: 2174 1071 858 742 399 330 85 BspEI T'CCGG_A Cuts at: 5322 5322 Size: 5659 BspHI T'CATG_A Cuts at: 3548 5278 3548 Size: 1730 3929 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3014 844 Size: 2170 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3546 1394 Size: 429 1723 3507 Fragments arranged by size: 3507 1723 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4154 4328 65 Size: 1183 2906 174 1396 Fragments arranged by size: 2906 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4303 4490 1700 Size: 110 2493 187 2869 Fragments arranged by size: 2869 2493 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5659 BssHII G'CGCG_C Cuts at: 1925 2093 2095 2097 1925 Size: 168 2 2 5487 Fragments arranged by size: 5487 168 2 2 BssSI C'ACGA_G Cuts at: 3719 4731 3719 Size: 1012 4647 BstAPI GCAn_nnn'nTGC Cuts at: 794 2059 794 Size: 1265 4394 BstBI TT'CG_AA Cuts at: 4463 4463 Size: 5659 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5659 BstYI r'GATC_y Cuts at: 1972 2245 2673 2989 3356 3741 3758 5210 5296 Size: 273 428 316 367 385 17 1452 86 Cuts at: 5296 5308 5654 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 428 385 367 346 316 273 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4168 Fragments arranged by size: 4168 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2013 2409 487 Size: 683 56 787 396 3737 Fragments arranged by size: 3737 787 683 396 56 BtsI GCAGTG_nn' Cuts at: 2171 3175 3969 3989 2171 Size: 1004 794 20 3841 Fragments arranged by size: 3841 1004 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5659 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5624 DraI TTT'AAA Cuts at: 2227 2963 2227 Size: 736 4923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4666 809 Size: 507 3350 1802 Fragments arranged by size: 3350 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3106 3995 5624 Size: 54 1425 50 275 18 1276 889 1629 Cuts at: 5624 8 Size: 43 Fragments arranged by size: 1629 1425 1276 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3106 1537 Size: 275 1294 4090 Fragments arranged by size: 4090 1294 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3589 1353 Size: 2236 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4246 4620 4766 387 Size: 1064 138 2657 374 146 1280 Fragments arranged by size: 2657 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2024 2630 2774 3722 4319 5106 5374 Size: 393 392 606 144 948 597 787 268 Cuts at: 5374 1239 Size: 1524 Fragments arranged by size: 1524 948 787 606 597 393 392 268 144 EcoNI CCTnn'n_nnAGG Cuts at: 2648 2648 Size: 5659 EcoO109I rG'GnC_Cy Cuts at: 2699 2699 Size: 5659 EcoRI G'AATT_C Cuts at: 2968 3346 2968 Size: 378 5281 FspI TGC'GCA Cuts at: 4165 4165 Size: 5659 HaeII r_GCGC'y Cuts at: 1678 2237 4806 1678 Size: 559 2569 2531 Fragments arranged by size: 2569 2531 559 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2237 2269 2419 2451 2453 Size: 32 135 32 665 32 150 32 2 Cuts at: 2453 2485 4300 4332 4374 4406 1373 Size: 32 1815 32 42 32 2626 Fragments arranged by size: 2626 1815 665 150 135 42 32 32 32 32 32 32 32 2 HincII GTy'rAC Cuts at: 669 1657 1938 2353 2438 3003 3249 669 Size: 988 281 415 85 565 246 3079 Fragments arranged by size: 3079 988 565 415 281 246 85 HindIII A'AGCT_T Cuts at: 748 748 Size: 5659 HpaI GTT'AAC Cuts at: 2353 3249 2353 Size: 896 4763 KpnI G_GTAC'C Cuts at: 2984 2984 Size: 5659 MfeI C'AATT_G Cuts at: 3258 3258 Size: 5659 MluI A'CGCG_T Cuts at: 5535 5535 Size: 5659 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4773 4957 1305 Size: 3468 184 2007 Fragments arranged by size: 3468 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3837 Fragments arranged by size: 3837 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3617 3976 4135 518 Size: 648 2451 359 159 2042 Fragments arranged by size: 2451 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2044 2154 2191 2778 3075 3752 4900 5145 Size: 438 110 37 587 297 677 1148 245 Cuts at: 5145 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 587 438 297 245 110 37 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4168 Fragments arranged by size: 4168 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5659 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5659 NotI GC'GGCC_GC Cuts at: 3106 3106 Size: 5659 NsiI A_TGCA'T Cuts at: 2688 2688 Size: 5659 NspI r_CATG'y Cuts at: 1132 1229 3017 4562 1132 Size: 97 1788 1545 2229 Fragments arranged by size: 2229 1788 1545 97 PasI CC'CwG_GG Cuts at: 1348 2014 1348 Size: 666 4993 PciI A'CATG_T Cuts at: 4558 4558 Size: 5659 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5659 PfoI T'CCnGG_A Cuts at: 2739 2945 2739 Size: 206 5453 PmeI GTTT'AAAC Cuts at: 2963 2963 Size: 5659 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3728 3760 5241 5273 3728 Size: 32 1481 32 4114 Fragments arranged by size: 4114 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2699 2699 Size: 5659 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5659 PsiI TTA'TAA Cuts at: 3229 3229 Size: 5659 PstI C_TGCA'G Cuts at: 830 2055 3011 830 Size: 1225 956 3478 Fragments arranged by size: 3478 1225 956 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5659 PvuII CAG'CTG Cuts at: 2044 2778 2044 Size: 734 4925 SalI G'TCGA_C Cuts at: 1936 2436 3001 1936 Size: 500 565 4594 Fragments arranged by size: 4594 565 500 SanDI GG'GwC_CC Cuts at: 2699 2699 Size: 5659 SbfI CC_TGCA'GG Cuts at: 3011 3011 Size: 5659 ScaI AGT'ACT Cuts at: 3907 5619 3907 Size: 1712 3947 SfcI C'TryA_G Cuts at: 826 1045 2051 3007 4142 4823 5014 826 Size: 219 1006 956 1135 681 191 1471 Fragments arranged by size: 1471 1135 1006 956 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5659 SmaI CCC'GGG Cuts at: 1872 2986 1872 Size: 1114 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2425 3763 4664 4926 820 Size: 197 926 482 1338 901 262 1553 Fragments arranged by size: 1553 1338 926 901 482 262 197 SnaBI TAC'GTA Cuts at: 493 3450 493 Size: 2957 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5659 SphI G_CATG'C Cuts at: 1132 3017 1132 Size: 1885 3774 SspI AAT'ATT Cuts at: 5 52 3583 5 Size: 47 3531 2081 Fragments arranged by size: 3531 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5659 StyI C'CwwG_G Cuts at: 513 1065 2004 2544 3098 513 Size: 552 939 540 554 3074 Fragments arranged by size: 3074 939 554 552 540 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2846 3686 3703 3856 4041 4486 325 Size: 1255 1266 840 17 153 185 445 1498 Fragments arranged by size: 1498 1266 1255 840 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2772 3905 5617 Size: 274 80 33 51 1443 795 1133 1712 Cuts at: 5617 96 Size: 138 Fragments arranged by size: 1712 1443 1133 795 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2691 2723 5619 5651 2691 Size: 32 2896 32 2699 Fragments arranged by size: 2896 2699 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5659 XbaI T'CTAG_A Cuts at: 2995 2995 Size: 5659 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2953 2953 Size: 5659 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5659 XmaI C'CCGG_G Cuts at: 1870 2984 1870 Size: 1114 4545 XmnI GAAnn'nnTTC Cuts at: 3788 3788 Size: 5659 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3467 276 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI