################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AccIII AclI AcsI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI AspEI AspI AvaI AvrII BamHI BanII BbeI BcgI BciVI BclI BfrI BglI BglII BlnI BsaBI BsaWI BseAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssSI Bst1107I BstAPI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HaeII HgaI HincII HindII HindIII HpaI HphI KasI Ksp632I MaeIII MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TfiI Tsp45I Tsp509I Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AatII 118 GACGTC GACGT^C Acc65I 305 GGTACC G^GTACC AccI 397 GTCTAC GT^[AC][GT]AC AcyI 118 GACGTC G[GA]^CG[CT]C AfaI 306 GTAC GT^AC Alw44I 592 GTGCAC G^TGCAC ApaLI 592 GTGCAC G^TGCAC Asp718I 305 GGTACC G^GTACC AspHI 592 GTGCAC G[AT]GC[AT]^C AviII 185 TGCGCA TGC^GCA BalI 331 TGGCCA TGG^CCA BanI 305 GGTACC G^G[CT][GA]CC BbrPI 589 CACGTG CAC^GTG BbsI 354 GAAGAC GAAGAC(2/6) BbvCI 309 CCTCAGC CCTCAGC(-5/-2) BlpI 219 GCTCAGC GC^T[ACGT]AGC BmyI 592 GTGCAC G[AGT]GC[ACT]^C Bpu1102I 219 GCTCAGC GC^T[ACGT]AGC BpuAI 354 GAAGAC GAAGAC(2/6) BsaAI 589 CACGTG [CT]AC^GT[GA] BsaHI 118 GACGTC G[GA]^CG[CT]C BsaI 440 GAGACC GGTCTC(1/5) BsiHKAI 592 GTGCAC G[AT]GC[AT]^C BsmAI 440 GAGAC GTCTC(1/5) Bsp1286I 592 GTGCAC G[AGT]GC[ACT]^C BspHI 867 TCATGA T^CATGA BssHII 201 GCGCGC G^CGCGC BstBI 729 TTCGAA TT^CGAA BstYI 680 AGATCC [GA]^GATC[CT] CelII 219 GCTCAGC GC^T[ACGT]AGC DraIII 637 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoT14I 752 CCAAGG C^C[AT][AT]GG FspI 185 TGCGCA TGC^GCA Hin1I 118 GACGTC G[GA]^CG[CT]C KpnI 305 GGTACC GGTAC^C KspI 245 CCGCGG CCGC^GG MflI 680 AGATCC [GA]^GATC[CT] MluNI 331 TGGCCA TGG^CCA MscI 331 TGGCCA TGG^CCA MseI 743 TTAA T^TAA NotI 241 GCGGCCGC GC^GGCCGC NspV 729 TTCGAA TT^CGAA PmaCI 589 CACGTG CAC^GTG PmlI 589 CACGTG CAC^GTG RcaI 867 TCATGA T^CATGA RsaI 306 GTAC GT^AC SacII 245 CCGCGG CCGC^GG SfcI 399 CTACAG C^T[GA][CT]AG SfuI 729 TTCGAA TT^CGAA SspI 860 AATATT AAT^ATT StyI 752 CCAAGG C^C[AT][AT]GG Tru9I 743 TTAA T^TAA XhoII 680 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AlwI 681 GATCC GGATC(4/5) 832 GATCC Asp700I 859 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 882 GAAGTTCTTC BcnI 783 CCGGG CC^[GC]GG 813 CCGGG BfaI 49 CTAG C^TAG 73 CTAG BpmI 109 CTCCAG CTGGAG(16/14) 211 CTGGAG BseRI 277 GAGGAG GAGGAG(10/8) 523 GAGGAG BsgI 226 CTGCAC GTGCAG(16/14) 853 CTGCAC DsaI 245 CCGCGG C^C[GA][CT]GG 291 CCACGG EagI 242 CGGCCG C^GGCCG 780 CGGCCG EclXI 242 CGGCCG C^GGCCG 780 CGGCCG Eco52I 242 CGGCCG C^GGCCG 780 CGGCCG HinfI 395 GAGTC G^A[ACGT]TC 802 GAGTC MaeI 49 CTAG C^TAG 73 CTAG MslI 177 CATCGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 471 CACCCAAGTG NciI 783 CCGGG CC^[GC]GG 813 CCGGG NspI 491 ACATGC [GA]CATG^[CT] 596 ACATGC PflMI 291 CCACGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 636 CCACATTGTGG PleI 395 GAGTC GAGTC(4/5) 802 GAGTC PpuMI 103 GGGACCC [GA]G^G[AT]CC[CT] 665 GGGACCC SfaNI 216 GATGC GCATC(5/9) 318 GATGC TspRI 206 CACTG CA[GC]TG[ACGT][ACGT]^ 347 CAGTG Van91I 291 CCACGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 636 CCACATTGTGG XmnI 859 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 882 GAAGTTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AccII AciI AluI AvaII BbvI BsaJI BsiEI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TaqI TseI TthHB8I