(Circular) (Six-base) MAPSORT of: pFN21AE2696.seq Check: 3242 from: 1 to: 5626 pFN21AE2696 5626 bp With 182 enzymes: SgfI * June 29, 2017 18:09 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2127 3436 278 Size: 53 83 186 936 591 1309 2468 Fragments arranged by size: 2468 1309 936 591 186 83 53 AccI GT'mk_AC Cuts at: 1937 2403 2969 1937 Size: 466 566 4594 Fragments arranged by size: 4594 566 466 Acc65I G'GTAC_C Cuts at: 2310 2947 2310 Size: 637 4989 AclI AA'CG_TT Cuts at: 1563 3753 4126 1563 Size: 2190 373 3063 Fragments arranged by size: 3063 2190 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3689 5073 5341 1632 Size: 2057 1384 268 1917 Fragments arranged by size: 2057 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5429 AflIII A'CryG_T Cuts at: 1203 4525 5502 1203 Size: 3322 977 1327 Fragments arranged by size: 3322 1327 977 AgeI A'CCGG_T Cuts at: 4457 4457 Size: 5626 AhdI GACnn_n'nnGTC Cuts at: 4355 4355 Size: 5626 AleI CACnn'nnGTG Cuts at: 1166 2480 1166 Size: 1314 4312 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2745 1184 Size: 1561 4065 AlwNI CAG_nnn'CTG Cuts at: 3369 4941 3369 Size: 1572 4054 ApaLI G'TGCA_C Cuts at: 2597 3683 4839 2597 Size: 1086 1156 3384 Fragments arranged by size: 3384 1156 1086 ApoI r'AATT_y Cuts at: 1493 2935 3161 3313 5422 1493 Size: 1442 226 152 2109 1697 Fragments arranged by size: 2109 1697 1442 226 152 AseI AT'TA_AT Cuts at: 160 1032 4180 160 Size: 872 3148 1606 Fragments arranged by size: 3148 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 AvaI C'yCGr_G Cuts at: 1870 1943 2951 1870 Size: 73 1008 4545 Fragments arranged by size: 4545 1008 73 BamHI G'GATC_C Cuts at: 2956 3323 2956 Size: 367 5259 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2310 2947 4402 618 Size: 325 217 627 523 637 1455 1842 Fragments arranged by size: 1842 1455 637 627 523 325 217 BanII G_rGCy'C Cuts at: 1391 2945 1391 Size: 1554 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2366 928 Size: 971 467 4188 Fragments arranged by size: 4188 971 467 BbvCI CC'TCA_GC Cuts at: 2315 2315 Size: 5626 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3817 3851 1903 Size: 34 1880 34 3678 Fragments arranged by size: 3678 1880 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3518 4728 3518 Size: 1210 4416 BclI T'GATC_A Cuts at: 1548 1548 Size: 5626 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4237 136 Size: 107 122 71 631 605 2565 1525 Fragments arranged by size: 2565 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5621 5621 Size: 5626 BlpI GC'TnA_GC Cuts at: 2225 3043 2225 Size: 818 4808 Bme1580I G_kGCm'C Cuts at: 1790 2601 3687 4843 1790 Size: 811 1086 1156 2573 Fragments arranged by size: 2573 1156 1086 811 BmgBI CAC'GTC Cuts at: 1318 2242 2379 1318 Size: 924 137 4565 Fragments arranged by size: 4565 924 137 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2287 3357 4315 Size: 416 53 83 399 24 870 1070 958 Cuts at: 4315 442 Size: 1753 Fragments arranged by size: 1753 1070 958 870 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5626 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 2237 4286 1239 Size: 860 138 2049 2579 Fragments arranged by size: 2579 2049 860 138 Bpu10I CC'TnA_GC Cuts at: 2315 2394 2315 Size: 79 5547 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3751 4616 4914 3751 Size: 865 298 4463 Fragments arranged by size: 4463 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2439 4289 882 Size: 617 940 1850 2219 Fragments arranged by size: 2219 1850 940 617 BsaAI yAC'GTr Cuts at: 493 1204 2596 3417 493 Size: 711 1392 821 2702 Fragments arranged by size: 2702 1392 821 711 BsaBI GATnn'nnATC Cuts at: 5620 5620 Size: 5626 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2124 3433 3815 4450 Size: 53 83 186 936 591 1309 382 635 Cuts at: 4450 275 Size: 1451 Fragments arranged by size: 1451 1309 936 635 591 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4059 4457 4731 4878 5289 4059 Size: 398 274 147 411 4396 Fragments arranged by size: 4396 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5401 Fragments arranged by size: 5401 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 2543 1186 Size: 1111 246 4269 Fragments arranged by size: 4269 1111 246 BseYI C'CCAG_C Cuts at: 2043 2049 2157 4829 5325 2043 Size: 6 108 2672 496 2344 Fragments arranged by size: 2672 2344 496 108 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2216 2843 1163 Size: 573 480 627 3946 Fragments arranged by size: 3946 627 573 480 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2250 2659 2788 3076 3837 4865 Size: 275 186 249 409 129 288 761 1028 Cuts at: 4865 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 409 288 275 249 186 129 BsiHKAI G_wGCw'C Cuts at: 2601 3687 3772 4843 2601 Size: 1086 85 1071 3384 Fragments arranged by size: 3384 1086 1071 85 BsmI GAATG_Cn' Cuts at: 3137 3230 3137 Size: 93 5533 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2601 2945 3687 3772 4843 1391 Size: 399 811 344 742 85 1071 2174 Fragments arranged by size: 2174 1071 811 742 399 344 85 BspEI T'CCGG_A Cuts at: 5289 5289 Size: 5626 BspHI T'CATG_A Cuts at: 2872 3515 5245 2872 Size: 643 1730 3253 Fragments arranged by size: 3253 1730 643 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2981 844 Size: 2137 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3513 1394 Size: 429 1690 3507 Fragments arranged by size: 3507 1690 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4121 4295 65 Size: 1183 2873 174 1396 Fragments arranged by size: 2873 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4270 4457 1700 Size: 110 2460 187 2869 Fragments arranged by size: 2869 2460 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5626 BssHII G'CGCG_C Cuts at: 1925 2206 1925 Size: 281 5345 BssSI C'ACGA_G Cuts at: 3686 4698 3686 Size: 1012 4614 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5626 BstBI TT'CG_AA Cuts at: 2735 4430 2735 Size: 1695 3931 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5626 BstYI r'GATC_y Cuts at: 1972 2685 2956 3323 3708 3725 5177 5263 5275 Size: 713 271 367 385 17 1452 86 12 Cuts at: 5275 5621 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 713 385 367 346 271 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2250 2296 513 Size: 552 939 246 46 3843 Fragments arranged by size: 3843 939 552 246 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2145 2559 487 Size: 683 56 919 414 3554 Fragments arranged by size: 3554 919 683 414 56 BtsI GCAGTG_nn' Cuts at: 2210 3142 3936 3956 2210 Size: 932 794 20 3880 Fragments arranged by size: 3880 932 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5626 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5591 DraI TTT'AAA Cuts at: 2930 2930 Size: 5626 DraIII CAC_nnn'GTG Cuts at: 2647 2647 Size: 5626 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4633 809 Size: 507 3317 1802 Fragments arranged by size: 3317 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2247 2336 Size: 54 1425 50 275 18 177 240 89 Cuts at: 2336 2785 3073 3962 5591 8 Size: 449 288 889 1629 43 Fragments arranged by size: 1629 1425 889 449 288 275 240 177 89 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2247 2785 3073 1537 Size: 275 435 538 288 4090 Fragments arranged by size: 4090 538 435 288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3556 1353 Size: 2203 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4213 4587 4733 387 Size: 1064 138 2624 374 146 1280 Fragments arranged by size: 2624 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2237 3689 4286 5073 5341 1239 Size: 393 467 138 1452 597 787 268 1524 Fragments arranged by size: 1524 1452 787 597 467 393 268 138 EcoO109I rG'GnC_Cy Cuts at: 2109 2572 2671 2109 Size: 463 99 5064 Fragments arranged by size: 5064 463 99 EcoRI G'AATT_C Cuts at: 2935 3313 2935 Size: 378 5248 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2908 2908 Size: 5626 FspI TGC'GCA Cuts at: 2192 4132 2192 Size: 1940 3686 HaeII r_GCGC'y Cuts at: 1678 4773 1678 Size: 3095 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4267 4299 4341 4373 1373 Size: 32 135 32 2695 32 42 32 2626 Fragments arranged by size: 2695 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2970 3216 669 Size: 988 281 1032 246 3079 Fragments arranged by size: 3079 1032 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5626 HpaI GTT'AAC Cuts at: 3216 3216 Size: 5626 KpnI G_GTAC'C Cuts at: 2314 2951 2314 Size: 637 4989 MfeI C'AATT_G Cuts at: 3225 3225 Size: 5626 MluI A'CGCG_T Cuts at: 5502 5502 Size: 5626 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2433 4740 4924 1305 Size: 1128 2307 184 2007 Fragments arranged by size: 2307 2007 1128 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2338 10 Size: 54 1425 343 506 3298 Fragments arranged by size: 3298 1425 506 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2186 2480 3584 3943 4102 518 Size: 648 1020 294 1104 359 159 2042 Fragments arranged by size: 2042 1104 1020 648 359 294 159 MspA1I CmG'CkG Cuts at: 1606 2252 2629 2784 2796 3042 3719 4867 5112 Size: 646 377 155 12 246 677 1148 245 Cuts at: 5112 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 646 377 246 245 155 12 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4135 Fragments arranged by size: 4135 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5626 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5626 NotI GC'GGCC_GC Cuts at: 2247 3073 2247 Size: 826 4800 NspI r_CATG'y Cuts at: 1132 1229 2500 2605 2984 4529 1132 Size: 97 1271 105 379 1545 2229 Fragments arranged by size: 2229 1545 1271 379 105 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5626 PciI A'CATG_T Cuts at: 4525 4525 Size: 5626 PflMI CCAn_nnn'nTGG Cuts at: 1108 2302 2647 1108 Size: 1194 345 4087 Fragments arranged by size: 4087 1194 345 PfoI T'CCnGG_A Cuts at: 2115 2115 Size: 5626 PmeI GTTT'AAAC Cuts at: 2930 2930 Size: 5626 PmlI CAC'GTG Cuts at: 2596 2596 Size: 5626 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3695 3727 5208 5240 3695 Size: 32 1481 32 4081 Fragments arranged by size: 4081 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2109 2671 2109 Size: 562 5064 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5626 PsiI TTA'TAA Cuts at: 3196 3196 Size: 5626 PstI C_TGCA'G Cuts at: 830 2978 830 Size: 2148 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5626 SacII CC_GC'GG Cuts at: 2253 2253 Size: 5626 SalI G'TCGA_C Cuts at: 1936 2968 1936 Size: 1032 4594 SanDI GG'GwC_CC Cuts at: 2109 2671 2109 Size: 562 5064 SbfI CC_TGCA'GG Cuts at: 2978 2978 Size: 5626 ScaI AGT'ACT Cuts at: 3874 5586 3874 Size: 1712 3914 SfcI C'TryA_G Cuts at: 826 1045 2404 2974 4109 4790 4981 826 Size: 219 1359 570 1135 681 191 1471 Fragments arranged by size: 1471 1359 1135 681 570 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5626 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5626 SmaI CCC'GGG Cuts at: 1872 2953 1872 Size: 1081 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3730 4631 4893 820 Size: 197 926 1787 901 262 1553 Fragments arranged by size: 1787 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3417 493 Size: 2924 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5626 SphI G_CATG'C Cuts at: 1132 2984 1132 Size: 1852 3774 SspI AAT'ATT Cuts at: 5 52 2867 3550 5 Size: 47 2815 683 2081 Fragments arranged by size: 2815 2081 683 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5626 StyI C'CwwG_G Cuts at: 513 1065 2004 2757 3065 513 Size: 552 939 753 308 3074 Fragments arranged by size: 3074 939 753 552 308 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2492 3653 3670 3823 4008 4453 325 Size: 1255 912 1161 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1161 912 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3872 5584 96 Size: 274 80 33 51 1443 1895 1712 138 Fragments arranged by size: 1895 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5586 5618 5586 Size: 32 5594 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5626 XbaI T'CTAG_A Cuts at: 2962 2962 Size: 5626 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5626 XmaI C'CCGG_G Cuts at: 1870 2951 1870 Size: 1081 4545 XmnI GAAnn'nnTTC Cuts at: 2868 2891 3755 2868 Size: 23 864 4739 Fragments arranged by size: 4739 864 23 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2125 3434 276 Size: 53 83 186 936 591 1309 2468 Fragments arranged by size: 2468 1309 936 591 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI XcmI