################################## # NO CUT ENZYMES ( 136 enzymes) # ################################## AatII Acc65I AccI AclI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbvCI BcgI BclI BfaI BfrI BglII BlnI BsaBI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstYI BstZ17I Cfr10I ClaI CpoI DpnII DraI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoRI EcoRV FbaI FseI FspI HaeII HincII HindII HpaI KasI KpnI Ksp632I KspI MaeI MamI MboI MflI MluI MluNI MscI NaeI NarI NdeI NdeII NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccII 892 CGCG CG^CG AccIII 842 TCCGGA T^CCGGA AcsI 675 GAATTT [GA]^AATT[CT] AcyI 505 GACGCC G[GA]^CG[CT]C AocI 294 CCTCAGG CC^T[ACGT]AGG ApaI 556 GGGCCC GGGCC^C ApoI 675 GAATTT [GA]^AATT[CT] AvaI 1077 CCCGAG C^[CT]CG[GA]G BanII 556 GGGCCC G[GA]GC[CT]^C BbrPI 277 CACGTG CAC^GTG BciVI 113 GGATAC GTATCC(6/5) BglI 422 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 556 GGGCCC G[AGT]GC[ACT]^C BpmI 354 CTCCAG CTGGAG(16/14) BsaAI 277 CACGTG [CT]AC^GT[GA] BsaHI 505 GACGCC G[GA]^CG[CT]C BsaI 793 GAGACC GGTCTC(1/5) BseAI 842 TCCGGA T^CCGGA BseRI 904 GAGGAG GAGGAG(10/8) BsoBI 1077 CCCGAG C^[CT]CG[GA]G Bsp1286I 556 GGGCCC G[AGT]GC[ACT]^C BspEI 842 TCCGGA T^CCGGA BsrDI 123 CATTGC GCAATG(2/0) BsrI 896 ACTGG ACTGG(1/-1) BssSI 638 CACGAG CACGAG(-5/-1) BstAPI 831 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 85 GGTAACC G^GT[ACGT]ACC BstPI 85 GGTAACC G^GT[ACGT]ACC BstUI 892 CGCG CG^CG Bsu36I 294 CCTCAGG CC^T[ACGT]AGG CfoI 1010 GCGC GCG^C DraIII 853 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 505 GACGCCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 1060 CGGCCA [CT]^GGCC[GA] Eco81I 294 CCTCAGG CC^T[ACGT]AGG EcoO65I 85 GGTAACC G^GT[ACGT]ACC EcoT22I 991 ATGCAT ATGCA^T HhaI 1010 GCGC GCG^C Hin1I 505 GACGCC G[GA]^CG[CT]C HinP1I 1010 GCGC G^CGC HindIII 362 AAGCTT A^AGCTT MaeII 278 ACGT A^CGT MfeI 144 CAATTG C^AATTG MroI 842 TCCGGA T^CCGGA MspA1I 539 CCGCTG C[AC]G^C[GT]G MunI 144 CAATTG C^AATTG MvnI 892 CGCG CG^CG NsiI 991 ATGCAT ATGCA^T PflMI 527 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 277 CACGTG CAC^GTG PmlI 277 CACGTG CAC^GTG PpuMI 570 AGGACCC [GA]G^G[AT]CC[CT] PstI 829 CTGCAG CTGCA^G SexAI 854 ACCAGGT A^CC[AT]GGT SfcI 829 CTGCAG C^T[GA][CT]AG TaqI 717 TCGA T^CGA TfiI 300 GATTC G^A[AT]TC TthHB8I 717 TCGA T^CGA Van91I 527 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 864 CCAAGTTGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AfaI 225 GTAC GT^AC 390 GTAC BlpI 541 GCTGAGC GC^T[ACGT]AGC 607 GCTGAGC Bpu1102I 541 GCTGAGC GC^T[ACGT]AGC 607 GCTGAGC BsaWI 842 TCCGGA [AT]^CCGG[AT] 930 ACCGGA BstXI 424 CCACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 776 CCAACGAGCTGG CelII 541 GCTGAGC GC^T[ACGT]AGC 607 GCTGAGC EcoT14I 109 CCATGG C^C[AT][AT]GG 1041 CCATGG HgaI 505 GACGC GACGC(5/10) 834 GACGC HphI 263 TCACC GGTGA(8/7) 996 TCACC MslI 91 CATTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 997 CACCCTGATG NcoI 109 CCATGG C^CATGG 1041 CCATGG RsaI 225 GTAC GT^AC 390 GTAC SfaNI 31 GATGC GCATC(5/9) 993 GCATC StyI 109 CCATGG C^C[AT][AT]GG 1041 CCATGG Tsp45I 479 GTCAC ^GT[GC]AC 485 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AluI AlwNI AvaII BbsI BbvI BcnI BpuAI BsaJI BsiYI BslI BsmAI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII DsaI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeIII MboII MnlI MseI MspI MvaI MwoI NciI NlaIII NlaIV PleI Sau96I ScrFI Tru9I TseI Tsp509I TspRI