Restriction map for
pFN21AE5744
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccI AccIII AflII
AflIII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI Eam1105I
Eco47III EcoO65I EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MamI MfeI MluI MroI MseI
MslI MunI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp45I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AclI 1356 AACGTT AA^CGTT
AfaI 1482 GTAC GT^AC
Alw44I 580 GTGCAC G^TGCAC
AocI 1191 CCTGAGG CC^T[ACGT]AGG
ApaLI 580 GTGCAC G^TGCAC
AspI 376 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1397 GGACC G^G[AT]CC
BalI 62 TGGCCA TGG^CCA
BanI 764 GGCACC G^G[CT][GA]CC
BciVI 1046 GTATCC GTATCC(6/5)
BfaI 1795 CTAG C^TAG
BglI 556 GCCTACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 970 GAGACC GGTCTC(1/5)
BsiEI 371 CGGCCG CG[GA][CT]^CG
BsmI 95 GCATTC GAATGC(1/-1)
BstAPI 252 GCAGGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1191 CCTGAGG CC^T[ACGT]AGG
EagI 371 CGGCCG C^GGCCG
EclXI 371 CGGCCG C^GGCCG
Eco52I 371 CGGCCG C^GGCCG
Eco81I 1191 CCTGAGG CC^T[ACGT]AGG
EcoNI 1509 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeI 1795 CTAG C^TAG
MluNI 62 TGGCCA TGG^CCA
MscI 62 TGGCCA TGG^CCA
NdeI 841 CATATG CA^TATG
PflFI 376 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1050 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1396 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 1356 AACGTT AA^CGTT
RsaI 1482 GTAC GT^AC
SphI 515 GCATGC GCATG^C
Tth111I 376 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1050 CCAGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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BbvCI 1155 CCTCAGC CCTCAGC(-5/-2)
1587 GCTGAGG
BsgI 55 GTGCAG GTGCAG(16/14)
682 GTGCAG
BsmBI 1124 CGTCTC CGTCTC(1/5)
43 GAGACG
BsmFI 806 GGGAC GGGAC(10/14)
1638 GGGAC
BsrBI 145 GAGCGG CCGCTC(-3/-3)
760 GAGCGG
BssSI 283 CACGAG CACGAG(-5/-1)
965 CACGAG
BstYI 461 AGATCC [GA]^GATC[CT]
1032 GGATCT
EaeI 62 TGGCCA [CT]^GGCC[GA]
371 CGGCCG
EarI 1660 GAAGAG CTCTTC(1/4)
1726 GAAGAG
EcoRI 891 GAATTC G^AATTC
1783 GAATTC
Ksp632I 1660 GAAGAG CTCTTC(1/4)
1726 GAAGAG
MaeIII 509 GTAAC ^GT[ACGT]AC
1623 GTTAC
MflI 461 AGATCC [GA]^GATC[CT]
1032 GGATCT
NlaIV 764 GGCACC GG[ACGT]^[ACGT]CC
1397 GGACCC
NspI 515 GCATGC [GA]CATG^[CT]
1305 GCATGT
SmaI 796 CCCGGG CCC^GGG
1475 CCCGGG
SmlI 214 CTCAAG C^T[CT][GA]AG
472 CTCAAG
TfiI 654 GATTC G^A[AT]TC
1645 GATTC
TspRI 896 CAGTG CA[GC]TG[ACGT][ACGT]^
1238 CACTG
XhoII 461 AGATCC [GA]^GATC[CT]
1032 GGATCT
XmaCI 796 CCCGGG C^CCGGG
1475 CCCGGG
XmaI 796 CCCGGG C^CCGGG
1475 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
AlwI AlwNI ApoI AspHI AvaI
BanII BbsI BbvI BcgI BcnI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I BspMI BsrI BssHII
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
ItaI MaeII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII PleI PstI
PvuII SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp509I TthHB8I