(Circular) (Six-base) MAPSORT of: pFN21AE5744.seq Check: 2083 from: 1 to: 6583 pFN21AE5744 6583 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3196 3517 3196 Size: 321 6262 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4393 278 Size: 53 83 186 936 2857 2468 Fragments arranged by size: 2857 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3926 1937 Size: 1989 4594 Acc65I G'GTAC_C Cuts at: 3904 3904 Size: 6583 AclI AA'CG_TT Cuts at: 1563 3362 4710 5083 1563 Size: 1799 1348 373 3063 Fragments arranged by size: 3063 1799 1348 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3011 3155 3764 4646 6030 6298 1632 Size: 1379 144 609 882 1384 268 1917 Fragments arranged by size: 1917 1384 1379 882 609 268 144 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6386 AflIII A'CryG_T Cuts at: 1203 5482 6459 1203 Size: 4279 977 1327 Fragments arranged by size: 4279 1327 977 AgeI A'CCGG_T Cuts at: 5414 5414 Size: 6583 AhdI GACnn_n'nnGTC Cuts at: 5312 5312 Size: 6583 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6583 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6583 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3176 3208 3176 Size: 32 6551 AlwNI CAG_nnn'CTG Cuts at: 2263 2517 2710 3211 3496 4326 5898 2263 Size: 254 193 501 285 830 1572 2948 Fragments arranged by size: 2948 1572 830 501 285 254 193 ApaLI G'TGCA_C Cuts at: 2585 4640 5796 2585 Size: 2055 1156 3372 Fragments arranged by size: 3372 2055 1156 ApoI r'AATT_y Cuts at: 1493 2896 3788 3875 3892 4118 4270 6379 1493 Size: 1403 892 87 17 226 152 2109 1697 Fragments arranged by size: 2109 1697 1403 892 226 152 87 17 AseI AT'TA_AT Cuts at: 160 1032 5137 160 Size: 872 4105 1606 Fragments arranged by size: 4105 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 AvaI C'yCGr_G Cuts at: 1870 1943 2039 2801 3444 3480 3567 3908 1870 Size: 73 96 762 643 36 87 341 4545 Fragments arranged by size: 4545 762 643 341 96 87 73 36 BamHI G'GATC_C Cuts at: 3913 4280 3913 Size: 367 6216 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2769 3904 5359 618 Size: 325 217 627 982 1135 1455 1842 Fragments arranged by size: 1842 1455 1135 982 627 325 217 BanII G_rGCy'C Cuts at: 1391 2220 2277 2478 2796 2826 3902 1391 Size: 829 57 201 318 30 1076 4072 Fragments arranged by size: 4072 1076 829 318 201 57 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2249 2925 3429 928 Size: 971 350 676 504 4082 Fragments arranged by size: 4082 971 676 504 350 BbvCI CC'TCA_GC Cuts at: 3161 3593 3161 Size: 432 6151 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2089 2123 2416 2450 2537 2571 4774 Size: 34 152 34 293 34 87 34 2203 Cuts at: 4774 4808 1903 Size: 34 3678 Fragments arranged by size: 3678 2203 293 152 87 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3062 4475 5685 3062 Size: 1413 1210 3960 Fragments arranged by size: 3960 1413 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6583 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2567 5194 136 Size: 107 122 71 631 605 895 2627 1525 Fragments arranged by size: 2627 1525 895 631 605 122 107 71 BglII A'GATC_T Cuts at: 6578 6578 Size: 6583 BlpI GC'TnA_GC Cuts at: 4000 4000 Size: 6583 Bme1580I G_kGCm'C Cuts at: 1790 2589 4644 5800 1790 Size: 799 2055 1156 2573 Fragments arranged by size: 2573 2055 1156 799 BmgBI CAC'GTC Cuts at: 1318 3226 1318 Size: 1908 4675 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4314 5272 442 Size: 416 53 83 399 24 2897 958 1753 Fragments arranged by size: 2897 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6583 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2192 2492 3233 3266 3503 5243 1239 Size: 953 300 741 33 237 1740 2579 Fragments arranged by size: 2579 1740 953 741 300 237 33 Bpu10I CC'TnA_GC Cuts at: 3161 3593 3161 Size: 432 6151 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2204 2462 4708 5573 5871 2204 Size: 258 2246 865 298 2916 Fragments arranged by size: 2916 2246 865 298 258 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2969 5246 882 Size: 617 1470 2277 2219 Fragments arranged by size: 2277 2219 1470 617 BsaAI yAC'GTr Cuts at: 493 1204 4374 493 Size: 711 3170 2702 Fragments arranged by size: 3170 2702 711 BsaBI GATnn'nnATC Cuts at: 6577 6577 Size: 6583 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2832 3128 3762 4390 Size: 53 83 186 936 1299 296 634 628 Cuts at: 4390 4772 5407 275 Size: 382 635 1451 Fragments arranged by size: 1451 1299 936 635 634 628 382 296 186 83 53 BsaWI w'CCGG_w Cuts at: 5016 5414 5688 5835 6246 5016 Size: 398 274 147 411 5353 Fragments arranged by size: 5353 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3176 3206 1178 Size: 30 165 30 1773 30 4555 Fragments arranged by size: 4555 1773 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2252 2432 2516 3080 3356 3464 1186 Size: 904 162 180 84 564 276 108 4305 Fragments arranged by size: 4305 904 564 276 180 162 108 84 BseYI C'CCAG_C Cuts at: 2693 3406 5786 6282 2693 Size: 713 2380 496 2994 Fragments arranged by size: 2994 2380 713 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 2708 1163 Size: 573 345 627 5038 Fragments arranged by size: 5038 627 573 345 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2379 4033 4794 5822 1540 Size: 275 186 378 1654 761 1028 2301 Fragments arranged by size: 2301 1654 1028 761 378 275 186 BsiHKAI G_wGCw'C Cuts at: 2220 2478 2589 2796 2826 4644 4729 5800 2220 Size: 258 111 207 30 1818 85 1071 3003 Fragments arranged by size: 3003 1818 1071 258 207 111 85 30 BsmI GAATG_Cn' Cuts at: 2100 4094 4187 2100 Size: 1994 93 4496 Fragments arranged by size: 4496 1994 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2042 3135 2042 Size: 1093 5490 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2220 2277 2478 2589 2796 2826 3902 Size: 399 430 57 201 111 207 30 1076 Cuts at: 3902 4644 4729 5800 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1076 1071 742 430 399 207 201 111 85 57 30 BspEI T'CCGG_A Cuts at: 6246 6246 Size: 6583 BspHI T'CATG_A Cuts at: 4472 6202 4472 Size: 1730 4853 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2559 3196 3468 3517 3938 844 Size: 1715 637 272 49 421 3489 Fragments arranged by size: 3489 1715 637 421 272 49 BsrBI CCG'CTC Cuts at: 1394 1823 2152 2767 4470 1394 Size: 429 329 615 1703 3507 Fragments arranged by size: 3507 1703 615 429 329 BsrDI GCAATG_nn' Cuts at: 65 1248 5078 5252 65 Size: 1183 3830 174 1396 Fragments arranged by size: 3830 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5227 5414 1700 Size: 110 3417 187 2869 Fragments arranged by size: 3417 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6583 BssHII G'CGCG_C Cuts at: 1925 2303 2411 2423 2486 1925 Size: 378 108 12 63 6022 Fragments arranged by size: 6022 378 108 63 12 BssSI C'ACGA_G Cuts at: 2288 2970 4643 5655 2288 Size: 682 1673 1012 3216 Fragments arranged by size: 3216 1673 1012 682 BstAPI GCAn_nnn'nTGC Cuts at: 794 2263 794 Size: 1469 5114 BstBI TT'CG_AA Cuts at: 5387 5387 Size: 6583 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6583 BstYI r'GATC_y Cuts at: 1972 2466 3037 3913 4280 4665 4682 6134 6220 Size: 494 571 876 367 385 17 1452 86 Cuts at: 6220 6232 6578 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 876 571 494 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3197 3197 Size: 6583 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6031 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5844 Fragments arranged by size: 5844 683 56 BtsI GCAGTG_nn' Cuts at: 4099 4893 4913 4099 Size: 794 20 5769 Fragments arranged by size: 5769 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6583 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6548 DraI TTT'AAA Cuts at: 3887 3887 Size: 6583 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5590 809 Size: 507 4274 1802 Fragments arranged by size: 4274 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2376 4030 Size: 54 1425 50 275 18 237 309 1654 Cuts at: 4030 4919 6548 8 Size: 889 1629 43 Fragments arranged by size: 1654 1629 1425 889 309 275 237 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2376 4030 1537 Size: 275 564 1654 4090 Fragments arranged by size: 4090 1654 564 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3660 3726 4513 1353 Size: 2307 66 787 3423 Fragments arranged by size: 3423 2307 787 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5170 5544 5690 387 Size: 1064 138 3581 374 146 1280 Fragments arranged by size: 3581 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2218 2476 2794 2824 2218 Size: 258 318 30 5977 Fragments arranged by size: 5977 318 258 30 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2492 3011 3155 3233 3266 3503 Size: 393 560 300 519 144 78 33 237 Cuts at: 3503 3764 4646 5243 6030 6298 1239 Size: 261 882 597 787 268 1524 Fragments arranged by size: 1524 882 787 597 560 519 393 300 268 261 237 144 78 33 EcoNI CCTnn'n_nnAGG Cuts at: 3518 3518 Size: 6583 EcoO109I rG'GnC_Cy Cuts at: 2430 3402 3493 2430 Size: 972 91 5520 Fragments arranged by size: 5520 972 91 EcoRI G'AATT_C Cuts at: 2896 3788 3892 4270 2896 Size: 892 104 378 5209 Fragments arranged by size: 5209 892 378 104 FspI TGC'GCA Cuts at: 5089 5089 Size: 6583 HaeII r_GCGC'y Cuts at: 1678 2264 2399 2449 2510 5730 1678 Size: 586 135 50 61 3220 2531 Fragments arranged by size: 3220 2531 586 135 61 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3224 3256 3329 3361 3807 Size: 32 135 32 1652 32 73 32 446 Cuts at: 3807 3839 5224 5256 5298 5330 1373 Size: 32 1385 32 42 32 2626 Fragments arranged by size: 2626 1652 1385 446 135 73 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3927 4173 669 Size: 988 281 1989 246 3079 Fragments arranged by size: 3079 1989 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6583 HpaI GTT'AAC Cuts at: 4173 4173 Size: 6583 KpnI G_GTAC'C Cuts at: 3908 3908 Size: 6583 MfeI C'AATT_G Cuts at: 4182 4182 Size: 6583 MluI A'CGCG_T Cuts at: 6459 6459 Size: 6583 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5697 5881 1305 Size: 4392 184 2007 Fragments arranged by size: 4392 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2069 10 Size: 54 1425 343 237 4524 Fragments arranged by size: 4524 1425 343 237 54 MslI CAynn'nnrTG Cuts at: 518 1166 4541 4900 5059 518 Size: 648 3375 359 159 2042 Fragments arranged by size: 3375 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2338 2743 2989 3475 3676 3742 3999 4676 Size: 732 405 246 486 201 66 257 677 Cuts at: 4676 5824 6069 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 732 677 486 405 257 246 245 201 66 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6031 NdeI CA'TA_TG Cuts at: 387 2847 387 Size: 2460 4123 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6583 NotI GC'GGCC_GC Cuts at: 4030 4030 Size: 6583 NspI r_CATG'y Cuts at: 1132 1229 2524 3314 3941 5486 1132 Size: 97 1295 790 627 1545 2229 Fragments arranged by size: 2229 1545 1295 790 627 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6583 PciI A'CATG_T Cuts at: 5482 5482 Size: 6583 PflMI CCAn_nnn'nTGG Cuts at: 1108 3061 1108 Size: 1953 4630 PfoI T'CCnGG_A Cuts at: 2469 2835 3519 2469 Size: 366 684 5533 Fragments arranged by size: 5533 684 366 PmeI GTTT'AAAC Cuts at: 3887 3887 Size: 6583 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3176 3208 4652 4684 6165 6197 3176 Size: 32 1444 32 1481 32 3562 Fragments arranged by size: 3562 1481 1444 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3402 3402 Size: 6583 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6583 PsiI TTA'TAA Cuts at: 4153 4153 Size: 6583 PstI C_TGCA'G Cuts at: 830 2160 2259 2337 2460 2721 3207 3935 830 Size: 1330 99 78 123 261 486 728 3478 Fragments arranged by size: 3478 1330 728 486 261 123 99 78 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6583 PvuII CAG'CTG Cuts at: 2743 2989 3475 3676 3742 2743 Size: 246 486 201 66 5584 Fragments arranged by size: 5584 486 246 201 66 SacI G_AGCT'C Cuts at: 2220 2478 2796 2826 2220 Size: 258 318 30 5977 Fragments arranged by size: 5977 318 258 30 SalI G'TCGA_C Cuts at: 1936 3925 1936 Size: 1989 4594 SbfI CC_TGCA'GG Cuts at: 3935 3935 Size: 6583 ScaI AGT'ACT Cuts at: 4831 6543 4831 Size: 1712 4871 SfcI C'TryA_G Cuts at: 826 1045 2156 2255 2333 2342 2456 2717 3203 Size: 219 1111 99 78 9 114 261 486 Cuts at: 3203 3931 5066 5747 5938 826 Size: 728 1135 681 191 1471 Fragments arranged by size: 1471 1135 1111 728 681 486 261 219 191 114 99 78 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6583 SmaI CCC'GGG Cuts at: 1872 2803 3482 3910 1872 Size: 931 679 428 4545 Fragments arranged by size: 4545 931 679 428 SmlI C'TyrA_G Cuts at: 820 1017 1943 2219 2477 4687 5588 5850 820 Size: 197 926 276 258 2210 901 262 1553 Fragments arranged by size: 2210 1553 926 901 276 262 258 197 SnaBI TAC'GTA Cuts at: 493 4374 493 Size: 3881 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6583 SphI G_CATG'C Cuts at: 1132 2524 3941 1132 Size: 1392 1417 3774 Fragments arranged by size: 3774 1417 1392 SspI AAT'ATT Cuts at: 5 52 4507 5 Size: 47 4455 2081 Fragments arranged by size: 4455 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6583 StyI C'CwwG_G Cuts at: 513 1065 2247 2538 2667 3765 4022 513 Size: 552 1182 291 129 1098 257 3074 Fragments arranged by size: 3074 1182 1098 552 291 257 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2420 2993 4610 4627 4780 4965 5410 Size: 1255 840 573 1617 17 153 185 445 Cuts at: 5410 325 Size: 1498 Fragments arranged by size: 1617 1498 1255 840 573 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4829 6541 96 Size: 274 80 33 51 1443 2852 1712 138 Fragments arranged by size: 2852 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2638 2670 3641 3673 6543 6575 2638 Size: 32 971 32 2870 32 2646 Fragments arranged by size: 2870 2646 971 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2384 1316 Size: 1068 5515 XbaI T'CTAG_A Cuts at: 3919 3919 Size: 6583 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6583 XmaI C'CCGG_G Cuts at: 1870 2801 3480 3908 1870 Size: 931 679 428 4545 Fragments arranged by size: 4545 931 679 428 XmnI GAAnn'nnTTC Cuts at: 4712 4712 Size: 6583 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4391 276 Size: 53 83 186 936 2857 2468 Fragments arranged by size: 2857 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI