(Linear) (Six-base) MAPSORT of: KIEE5744.seq Check: 5656 from: 1 to: 1878 KIEE5744.orf With 182 enzymes: SgfI * December 5, 2014 13:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1191 1512 1878 Size: 1191 321 366 Fragments arranged by size: 1191 366 321 AclI AA'CG_TT Cuts at: 0 1357 1878 Size: 1357 521 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1006 1150 1759 1878 Size: 1006 144 609 119 Fragments arranged by size: 1006 609 144 119 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1171 1203 1878 Size: 1171 32 675 Fragments arranged by size: 1171 675 32 AlwNI CAG_nnn'CTG Cuts at: 0 258 512 705 1206 1491 1878 Size: 258 254 193 501 285 387 Fragments arranged by size: 501 387 285 258 254 193 ApaLI G'TGCA_C Cuts at: 0 580 1878 Size: 580 1298 ApoI r'AATT_y Cuts at: 0 891 1783 1870 1878 Size: 891 892 87 8 Fragments arranged by size: 892 891 87 8 AvaI C'yCGr_G Cuts at: 0 34 796 1439 1475 1562 1878 Size: 34 762 643 36 87 316 Fragments arranged by size: 762 643 316 87 36 34 BanI G'GyrC_C Cuts at: 0 764 1878 Size: 764 1114 BanII G_rGCy'C Cuts at: 0 215 272 473 791 821 1878 Size: 215 57 201 318 30 1057 Fragments arranged by size: 1057 318 215 201 57 30 BbsI GAAGACnn'nnnn_ Cuts at: 0 244 920 1424 1878 Size: 244 676 504 454 Fragments arranged by size: 676 504 454 244 BbvCI CC'TCA_GC Cuts at: 0 1156 1588 1878 Size: 1156 432 290 Fragments arranged by size: 1156 432 290 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 84 118 411 445 532 566 1878 Size: 84 34 293 34 87 34 1312 Fragments arranged by size: 1312 293 87 84 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1057 1878 Size: 1057 821 BglI GCCn_nnn'nGGC Cuts at: 0 562 1878 Size: 562 1316 Bme1580I G_kGCm'C Cuts at: 0 584 1878 Size: 584 1294 BmgBI CAC'GTC Cuts at: 0 1221 1878 Size: 1221 657 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 187 487 1228 1261 1498 1878 Size: 187 300 741 33 237 380 Fragments arranged by size: 741 380 300 237 187 33 Bpu10I CC'TnA_GC Cuts at: 0 1156 1588 1878 Size: 1156 432 290 Fragments arranged by size: 1156 432 290 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 457 1878 Size: 199 258 1421 Fragments arranged by size: 1421 258 199 BsaI GGTCTCn'nnnn_ Cuts at: 0 964 1878 Size: 964 914 BsaHI Gr'CG_yC Cuts at: 0 827 1123 1757 1878 Size: 827 296 634 121 Fragments arranged by size: 827 634 296 121 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1171 1201 1878 Size: 1171 30 677 Fragments arranged by size: 1171 677 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 85 247 427 511 1075 1351 1459 1878 Size: 85 162 180 84 564 276 108 419 Fragments arranged by size: 564 419 276 180 162 108 85 84 BseYI C'CCAG_C Cuts at: 0 688 1401 1878 Size: 688 713 477 Fragments arranged by size: 713 688 477 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 703 1878 Size: 76 627 1175 Fragments arranged by size: 1175 627 76 BsiEI CG_ry'CG Cuts at: 0 374 1878 Size: 374 1504 BsiHKAI G_wGCw'C Cuts at: 0 215 473 584 791 821 1878 Size: 215 258 111 207 30 1057 Fragments arranged by size: 1057 258 215 207 111 30 BsmI GAATG_Cn' Cuts at: 0 95 1878 Size: 95 1783 BsmBI CGTCTCn'nnnn_ Cuts at: 0 37 1130 1878 Size: 37 1093 748 Fragments arranged by size: 1093 748 37 Bsp1286I G_dGCh'C Cuts at: 0 215 272 473 584 791 821 1878 Size: 215 57 201 111 207 30 1057 Fragments arranged by size: 1057 215 207 201 111 57 30 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 554 1191 1463 1512 1878 Size: 554 637 272 49 366 Fragments arranged by size: 637 554 366 272 49 BsrBI CCG'CTC Cuts at: 0 147 762 1878 Size: 147 615 1116 Fragments arranged by size: 1116 615 147 BssHII G'CGCG_C Cuts at: 0 298 406 418 481 1878 Size: 298 108 12 63 1397 Fragments arranged by size: 1397 298 108 63 12 BssSI C'ACGA_G Cuts at: 0 283 965 1878 Size: 283 682 913 Fragments arranged by size: 913 682 283 BstAPI GCAn_nnn'nTGC Cuts at: 0 258 1878 Size: 258 1620 BstYI r'GATC_y Cuts at: 0 461 1032 1878 Size: 461 571 846 Fragments arranged by size: 846 571 461 Bsu36I CC'TnA_GG Cuts at: 0 1192 1878 Size: 1192 686 EaeI y'GGCC_r Cuts at: 0 62 371 1878 Size: 62 309 1507 Fragments arranged by size: 1507 309 62 EagI C'GGCC_G Cuts at: 0 371 1878 Size: 371 1507 EarI CTCTTCn'nnn_ Cuts at: 0 1655 1721 1878 Size: 1655 66 157 Fragments arranged by size: 1655 157 66 EcoICRI GAG'CTC Cuts at: 0 213 471 789 819 1878 Size: 213 258 318 30 1059 Fragments arranged by size: 1059 318 258 213 30 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 187 487 1006 1150 1228 1261 1498 1759 Size: 187 300 519 144 78 33 237 261 Cuts at: 1759 1878 Size: 119 Fragments arranged by size: 519 300 261 237 187 144 119 78 33 EcoNI CCTnn'n_nnAGG Cuts at: 0 1513 1878 Size: 1513 365 EcoO109I rG'GnC_Cy Cuts at: 0 425 1397 1488 1878 Size: 425 972 91 390 Fragments arranged by size: 972 425 390 91 EcoRI G'AATT_C Cuts at: 0 891 1783 1878 Size: 891 892 95 Fragments arranged by size: 892 891 95 HaeII r_GCGC'y Cuts at: 0 259 394 444 505 1878 Size: 259 135 50 61 1373 Fragments arranged by size: 1373 259 135 61 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1219 1251 1324 1356 1802 1834 1878 Size: 1219 32 73 32 446 32 44 Fragments arranged by size: 1219 446 73 44 32 32 32 MscI TGG'CCA Cuts at: 0 64 1878 Size: 64 1814 MspA1I CmG'CkG Cuts at: 0 333 738 984 1470 1671 1737 1878 Size: 333 405 246 486 201 66 141 Fragments arranged by size: 486 405 333 246 201 141 66 NdeI CA'TA_TG Cuts at: 0 842 1878 Size: 842 1036 NspI r_CATG'y Cuts at: 0 519 1309 1878 Size: 519 790 569 Fragments arranged by size: 790 569 519 PflMI CCAn_nnn'nTGG Cuts at: 0 1056 1878 Size: 1056 822 PfoI T'CCnGG_A Cuts at: 0 464 830 1514 1878 Size: 464 366 684 364 Fragments arranged by size: 684 464 366 364 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1171 1203 1878 Size: 1171 32 675 Fragments arranged by size: 1171 675 32 PpuMI rG'GwC_Cy Cuts at: 0 1397 1878 Size: 1397 481 PstI C_TGCA'G Cuts at: 0 155 254 332 455 716 1202 1878 Size: 155 99 78 123 261 486 676 Fragments arranged by size: 676 486 261 155 123 99 78 PvuII CAG'CTG Cuts at: 0 738 984 1470 1671 1737 1878 Size: 738 246 486 201 66 141 Fragments arranged by size: 738 486 246 201 141 66 SacI G_AGCT'C Cuts at: 0 215 473 791 821 1878 Size: 215 258 318 30 1057 Fragments arranged by size: 1057 318 258 215 30 SfcI C'TryA_G Cuts at: 0 151 250 328 337 451 712 1198 1878 Size: 151 99 78 9 114 261 486 680 Fragments arranged by size: 680 486 261 151 114 99 78 9 SmaI CCC'GGG Cuts at: 0 798 1477 1878 Size: 798 679 401 Fragments arranged by size: 798 679 401 SmlI C'TyrA_G Cuts at: 0 214 472 1878 Size: 214 258 1406 Fragments arranged by size: 1406 258 214 SphI G_CATG'C Cuts at: 0 519 1878 Size: 519 1359 StyI C'CwwG_G Cuts at: 0 242 533 662 1760 1878 Size: 242 291 129 1098 118 Fragments arranged by size: 1098 291 242 129 118 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 415 988 1878 Size: 415 573 890 Fragments arranged by size: 890 573 415 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 633 665 1636 1668 1878 Size: 633 32 971 32 210 Fragments arranged by size: 971 633 210 32 32 Tth111I GACn'n_nGTC Cuts at: 0 379 1878 Size: 379 1499 XmaI C'CCGG_G Cuts at: 0 796 1475 1878 Size: 796 679 403 Fragments arranged by size: 796 679 403 Enzymes that do cut: AarI AclI AcuI AloI AlwNI ApaLI ApoI AvaI BanI BanII BbsI BbvCI BcgI BciVI BglI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BssHII BssSI BstAPI BstYI Bsu36I EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I MscI MspA1I NdeI NspI PflMI PfoI PpiI PpuMI PstI PvuII SacI SfcI SmaI SmlI SphI StyI TaqII TstI Tth111I XmaI Enzymes that do not cut: AatII AccI Acc65I AfeI AflII AflIII AgeI AhdI AleI AlfI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI BclI BfrBI BglII BlpI BmrI BmtI BplI BsaAI BsaBI BsaWI BsiWI BspEI BspHI BsrDI BsrFI BsrGI BstBI BstEII BstXI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EciI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MslI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI TatI XbaI XcmI XhoI XmnI ZraI