Restriction map for
pFN21AE2740
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI MamI MfeI
MluI MroI MseI MslI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp509I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccI 118 GTCTAC GT^[AC][GT]AC
AflIII 1082 ACGTGT A^C[GA][CT]GT
Alw44I 1155 GTGCAC G^TGCAC
AlwNI 902 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1155 GTGCAC G^TGCAC
BamHI 308 GGATCC G^GATCC
BbsI 541 GTCTTC GAAGAC(2/6)
BpuAI 541 GTCTTC GAAGAC(2/6)
BsaI 1247 GAGACC GGTCTC(1/5)
BsgI 391 CTGCAC GTGCAG(16/14)
BsmBI 1347 GAGACG CGTCTC(1/5)
BspMI 1159 ACCTGC ACCTGC(4/8)
BsrBI 375 GAGCGG CCGCTC(-3/-3)
BsrDI 401 GCAATG GCAATG(2/0)
BstAPI 1226 GCAGCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 308 GGATCC [GA]^GATC[CT]
DrdI 589 GACTGCATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 57 GAAGAG CTCTTC(1/4)
EcoNI 246 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Ksp632I 57 GAAGAG CTCTTC(1/4)
KspI 970 CCGCGG CCGC^GG
MflI 308 GGATCC [GA]^GATC[CT]
NaeI 836 GCCGGC GCC^GGC
NcoI 929 CCATGG C^CATGG
NgoMIV 836 GCCGGC G^CCGGC
PpuMI 666 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 652 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 979 CTGCAG CTGCA^G
SacI 999 GAGCTC GAGCT^C
SacII 970 CCGCGG CCGC^GG
TfiI 497 GATTC G^A[AT]TC
XcmI 1094 CCACAGATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 308 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 35 enzymes) #
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AatII 820 GACGTC GACGT^C
1311 GACGTC
AfaI 528 GTAC GT^AC
1182 GTAC
AlwI 309 GATCC GGATC(4/5)
308 GGATC
AspHI 999 GAGCTC G[AT]GC[AT]^C
1155 GTGCAC
BlpI 732 GCTGAGC GC^T[ACGT]AGC
1116 GCTCAGC
Bpu1102I 732 GCTGAGC GC^T[ACGT]AGC
1116 GCTCAGC
BsiEI 557 CGGCCG CG[GA][CT]^CG
637 CGGCCG
BsiHKAI 999 GAGCTC G[AT]GC[AT]^C
1155 GTGCAC
BsmAI 1247 GAGAC GTCTC(1/5)
1347 GAGAC
BsrFI 639 GCCGGT [GA]^CCGG[CT]
836 GCCGGC
BssSI 280 CACGAG CACGAG(-5/-1)
1344 CACGAG
BstXI 257 CCATCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
809 CCAGTCACTTGG
CelII 732 GCTGAGC GC^T[ACGT]AGC
1116 GCTCAGC
Cfr10I 639 GCCGGT [GA]^CCGG[CT]
836 GCCGGC
DpnII 309 GATC ^GATC
573 GATC
EagI 557 CGGCCG C^GGCCG
637 CGGCCG
EclXI 557 CGGCCG C^GGCCG
637 CGGCCG
Eco52I 557 CGGCCG C^GGCCG
637 CGGCCG
EcoT14I 617 CCAAGG C^C[AT][AT]GG
929 CCATGG
HphI 1362 GGTGA GGTGA(8/7)
842 TCACC
MboI 309 GATC ^GATC
573 GATC
NdeII 309 GATC ^GATC
573 GATC
NspI 747 ACATGC [GA]CATG^[CT]
1471 GCATGT
PflMI 225 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1477 CCAGTCTGTGG
PleI 1297 GACTC GAGTC(4/5)
1490 GACTC
RsaI 528 GTAC GT^AC
1182 GTAC
Sau3AI 309 GATC ^GATC
573 GATC
SexAI 517 ACCAGGT A^CC[AT]GGT
1184 ACCAGGT
SfcI 715 CTACAG C^T[GA][CT]AG
979 CTGCAG
SmaI 663 CCCGGG CCC^GGG
1168 CCCGGG
SmlI 942 CTCAAG C^T[CT][GA]AG
1492 CTCAAG
StyI 617 CCAAGG C^C[AT][AT]GG
929 CCATGG
Van91I 225 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1477 CCAGTCTGTGG
XmaCI 663 CCCGGG C^CCGGG
1168 CCCGGG
XmaI 663 CCCGGG C^CCGGG
1168 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII ItaI
MaeI MaeII MaeIII MboII MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PvuII Sau96I ScrFI SfaNI TaqI
TseI Tsp45I TspRI TthHB8I