Restriction map for
pFN21AE2740
(Circular) (Six-base) MAPSORT of: pFN21AE2740.seq Check: 7030 from: 1 to: 6217
pFN21AE2740 6217 bp
With 182 enzymes: SgfI *
December 17, 2018 11:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3173 3173
Size: 6217
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2829 3320 4027 278
Size: 53 83 186 936 1293 491 707 2468
Fragments arranged by size:
2468 1293 936 707 491 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2124 3560 1937
Size: 187 1436 4594
Fragments arranged by size:
4594 1436 187
Acc65I G'GTAC_C
Cuts at: 3538 3538
Size: 6217
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4280 5664 5932 1632
Size: 2648 1384 268 1917
Fragments arranged by size:
2648 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6020
AflIII A'CryG_T
Cuts at: 1203 3087 5116 6093 1203
Size: 1884 2029 977 1327
Fragments arranged by size:
2029 1884 1327 977
AgeI A'CCGG_T
Cuts at: 5048 5048
Size: 6217
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3185 1184
Size: 2001 4216
AlwNI CAG_nnn'CTG
Cuts at: 2912 3960 5532 2912
Size: 1048 1572 3597
Fragments arranged by size:
3597 1572 1048
ApaI G_GGCC'C
Cuts at: 2630 3145 3214 2630
Size: 515 69 5633
Fragments arranged by size:
5633 515 69
ApaLI G'TGCA_C
Cuts at: 3160 4274 5430 3160
Size: 1114 1156 3947
Fragments arranged by size:
3947 1156 1114
ApoI r'AATT_y
Cuts at: 1493 3526 3752 3904 6013 1493
Size: 2033 226 152 2109 1697
Fragments arranged by size:
2109 2033 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 2668 3146 3173 3542 1870
Size: 73 725 478 27 369 4545
Fragments arranged by size:
4545 725 478 369 73 27
BamHI G'GATC_C
Cuts at: 2313 3547 3914 2313
Size: 1234 367 4616
Fragments arranged by size:
4616 1234 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2366 2942 3193 3538 4993
Size: 325 217 627 579 576 251 345 1455
Cuts at: 4993 618
Size: 1842
Fragments arranged by size:
1842 1455 627 579 576 345 325 251
217
BanII G_rGCy'C
Cuts at: 1391 2205 2630 3008 3116 3145 3153 3214 3426
Size: 814 425 378 108 29 8 61 212
Cuts at: 3426 3536 1391
Size: 110 4072
Fragments arranged by size:
4072 814 425 378 212 110 108 61
29 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2539 928
Size: 971 640 4606
Fragments arranged by size:
4606 971 640
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4408 4442 1903
Size: 34 2471 34 3678
Fragments arranged by size:
3678 2471 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4109 5319 4109
Size: 1210 5007
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6217
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4828 136
Size: 107 122 71 631 605 3156 1525
Fragments arranged by size:
3156 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6212 6212
Size: 6217
BlpI GC'TnA_GC
Cuts at: 2738 3122 3634 2738
Size: 384 512 5321
Fragments arranged by size:
5321 512 384
Bme1580I G_kGCm'C
Cuts at: 1790 2630 3145 3164 3198 3214 3350 4278 5434
Size: 840 515 19 34 16 136 928 1156
Cuts at: 5434 1790
Size: 2573
Fragments arranged by size:
2573 1156 928 840 515 136 34 19
16
BmgBI CAC'GTC
Cuts at: 1318 2886 3088 1318
Size: 1568 202 4447
Fragments arranged by size:
4447 1568 202
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2545 3119 3948
Size: 416 53 83 399 24 1128 574 829
Cuts at: 3948 4906 442
Size: 958 1753
Fragments arranged by size:
1753 1128 958 829 574 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6217
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2884 2884
Size: 6217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2219 2346 2747 3034 4877 1239
Size: 980 127 401 287 1843 2579
Fragments arranged by size:
2579 1843 980 401 287 127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2932 3482 4342 5207 5505 2932
Size: 550 860 865 298 3644
Fragments arranged by size:
3644 865 860 550 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3246 4880 882
Size: 617 1747 1634 2219
Fragments arranged by size:
2219 1747 1634 617
BsaAI yAC'GTr
Cuts at: 493 1204 4008 493
Size: 711 2804 2702
Fragments arranged by size:
2804 2702 711
BsaBI GATnn'nnATC
Cuts at: 6211 6211
Size: 6217
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2544 2826 3317 4024
Size: 53 83 186 936 1011 282 491 707
Cuts at: 4024 4406 5041 275
Size: 382 635 1451
Fragments arranged by size:
1451 1011 936 707 635 491 382 282
186 83 53
BsaWI w'CCGG_w
Cuts at: 4650 5048 5322 5469 5880 4650
Size: 398 274 147 411 4987
Fragments arranged by size:
4987 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2344 2374 1178
Size: 30 165 30 941 30 5021
Fragments arranged by size:
5021 941 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2702 2735 2998 3031 1186
Size: 1516 33 263 33 4372
Fragments arranged by size:
4372 1516 263 33 33
BseYI C'CCAG_C
Cuts at: 2269 3152 3343 5420 5916 2269
Size: 883 191 2077 496 2570
Fragments arranged by size:
2570 2077 883 496 191
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2381 1163
Size: 573 645 4999
Fragments arranged by size:
4999 645 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2565 2645 3667 4428 5456 1540
Size: 275 186 564 80 1022 761 1028 2301
Fragments arranged by size:
2301 1028 1022 761 564 275 186 80
BsiHKAI G_wGCw'C
Cuts at: 3008 3164 4278 4363 5434 3008
Size: 156 1114 85 1071 3791
Fragments arranged by size:
3791 1114 1071 156 85
BsmI GAATG_Cn'
Cuts at: 3728 3821 3728
Size: 93 6124
BsmBI CGTCTCn'nnnn_
Cuts at: 3346 3346
Size: 6217
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2205 2630 3008 3116 3145 3153 3164
Size: 399 415 425 378 108 29 8 11
Cuts at: 3164 3198 3214 3350 3426 3536 4278 4363 5434
Size: 34 16 136 76 110 742 85 1071
Cuts at: 5434 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 425 415 399 378 136
110 108 85 76 34 29 16 11
8
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 6217
BspHI T'CATG_A
Cuts at: 4106 5836 4106
Size: 1730 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3173 3572 844
Size: 2329 399 3489
Fragments arranged by size:
3489 2329 399
BsrBI CCG'CTC
Cuts at: 1394 1823 2382 4104 1394
Size: 429 559 1722 3507
Fragments arranged by size:
3507 1722 559 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2413 4712 4886 65
Size: 1183 1165 2299 174 1396
Fragments arranged by size:
2299 1396 1183 1165 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2644 2841 4861 5048 1700
Size: 110 834 197 2020 187 2869
Fragments arranged by size:
2869 2020 834 197 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6217
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6217
BssSI C'ACGA_G
Cuts at: 2285 3349 4277 5289 2285
Size: 1064 928 1012 3213
Fragments arranged by size:
3213 1064 1012 928
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3237 794
Size: 2443 3774
BstBI TT'CG_AA
Cuts at: 5021 5021
Size: 6217
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6217
BstXI CCAn_nnnn'nTGG
Cuts at: 2269 2821 2269
Size: 552 5665
BstYI r'GATC_y
Cuts at: 1972 2313 3547 3914 4299 4316 5768 5854 5866
Size: 341 1234 367 385 17 1452 86 12
Cuts at: 5866 6212 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1234 385 367 346 341 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2097 2934 2975 513
Size: 552 1032 837 41 3755
Fragments arranged by size:
3755 1032 837 552 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3007 487
Size: 683 56 1781 3697
Fragments arranged by size:
3697 1781 683 56
BtsI GCAGTG_nn'
Cuts at: 2462 2776 3116 3398 3733 4527 4547 2462
Size: 314 340 282 335 794 20 4132
Fragments arranged by size:
4132 794 340 335 314 282 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6182
DraI TTT'AAA
Cuts at: 3521 3521
Size: 6217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2600 5224 809
Size: 507 1284 2624 1802
Fragments arranged by size:
2624 1802 1284 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2238 2424 2562
Size: 54 1425 50 275 18 408 186 138
Cuts at: 2562 2642 2855 2995 3294 3310 3664 4553 6182
Size: 80 213 140 299 16 354 889 1629
Cuts at: 6182 8
Size: 43
Fragments arranged by size:
1629 1425 889 408 354 299 275 213
186 140 138 80 54 50 43 18
16
EagI C'GGCC_G
Cuts at: 1537 1812 2562 2642 3664 1537
Size: 275 750 80 1022 4090
Fragments arranged by size:
4090 1022 750 275 80
EarI CTCTTCn'nnn_
Cuts at: 1353 2057 4147 1353
Size: 704 2090 3423
Fragments arranged by size:
3423 2090 704
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3310 4804 5178 5324 387
Size: 1064 138 1721 1494 374 146 1280
Fragments arranged by size:
1721 1494 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3006 3006
Size: 6217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 2346 2747 3034 4280 4877 5664
Size: 393 587 127 401 287 1246 597 787
Cuts at: 5664 5932 1239
Size: 268 1524
Fragments arranged by size:
1524 1246 787 597 587 401 393 287
268 127
EcoNI CCTnn'n_nnAGG
Cuts at: 2255 2255
Size: 6217
EcoO109I rG'GnC_Cy
Cuts at: 2152 2221 2626 2627 2672 2897 3141 3142 3361
Size: 69 405 1 45 225 244 1 219
Cuts at: 3361 3376 2152
Size: 15 4993
Fragments arranged by size:
4993 405 244 225 219 69 45 15
1 1
EcoRI G'AATT_C
Cuts at: 3526 3904 3526
Size: 378 5839
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2647 2647
Size: 6217
FspI TGC'GCA
Cuts at: 4723 4723
Size: 6217
HaeII r_GCGC'y
Cuts at: 1678 2021 2482 2835 5364 1678
Size: 343 461 353 2529 2531
Fragments arranged by size:
2531 2529 461 353 343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2344 2376 4858 4890 4932
Size: 32 135 32 772 32 2482 32 42
Cuts at: 4932 4964 1373
Size: 32 2626
Fragments arranged by size:
2626 2482 772 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3561 3807 669
Size: 988 281 1623 246 3079
Fragments arranged by size:
3079 1623 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6217
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KpnI G_GTAC'C
Cuts at: 3542 3542
Size: 6217
MfeI C'AATT_G
Cuts at: 3816 3816
Size: 6217
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2118 2355 5331 5515 1305
Size: 813 237 2976 184 2007
Fragments arranged by size:
2976 2007 813 237 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2240 2426 2857 10
Size: 54 1425 343 408 186 431 3370
Fragments arranged by size:
3370 1425 431 408 343 186 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4175 4534 4693 518
Size: 648 3009 359 159 2042
Fragments arranged by size:
3009 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2725 2912 2968 2977 2983 3018 3137 3633
Size: 1119 187 56 9 6 35 119 496
Cuts at: 3633 4310 5458 5703 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1119 677 496 245 187 119
56 35 9 6
NaeI GCC'GGC
Cuts at: 2843 2843
Size: 6217
NcoI C'CATG_G
Cuts at: 513 1065 2934 513
Size: 552 1869 3796
Fragments arranged by size:
3796 1869 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NgoMIV G'CCGG_C
Cuts at: 2841 2841
Size: 6217
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6217
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 2756 3480 3575 5120 1132
Size: 97 1527 724 95 1545 2229
Fragments arranged by size:
2229 1545 1527 724 97 95
PasI CC'CwG_GG
Cuts at: 1348 3382 3426 1348
Size: 2034 44 4139
Fragments arranged by size:
4139 2034 44
PciI A'CATG_T
Cuts at: 5116 5116
Size: 6217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2236 3488 1108
Size: 1128 1252 3837
Fragments arranged by size:
3837 1252 1128
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2672 2672
Size: 6217
PshAI GACnn'nnGTC
Cuts at: 1613 2661 1613
Size: 1048 5169
PsiI TTA'TAA
Cuts at: 3787 3787
Size: 6217
PspOMI G'GGCC_C
Cuts at: 2626 3141 3210 2626
Size: 515 69 5633
Fragments arranged by size:
5633 515 69
PstI C_TGCA'G
Cuts at: 830 2988 3569 830
Size: 2158 581 3478
Fragments arranged by size:
3478 2158 581
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
PvuII CAG'CTG
Cuts at: 2725 2912 2983 2725
Size: 187 71 5959
Fragments arranged by size:
5959 187 71
SacI G_AGCT'C
Cuts at: 3008 3008
Size: 6217
SacII CC_GC'GG
Cuts at: 2978 2978
Size: 6217
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SanDI GG'GwC_CC
Cuts at: 2672 2672
Size: 6217
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 4465 6177 4465
Size: 1712 4505
SexAI A'CCwGG_T
Cuts at: 2522 3189 2522
Size: 667 5550
SfcI C'TryA_G
Cuts at: 826 1045 2720 2984 3565 4700 5381 5572 826
Size: 219 1675 264 581 1135 681 191 1471
Fragments arranged by size:
1675 1471 1135 681 581 264 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6217
SmaI CCC'GGG
Cuts at: 1872 2670 3175 3544 1872
Size: 798 505 369 4545
Fragments arranged by size:
4545 798 505 369
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2947 3497 4321 5222 5484 820
Size: 197 926 1004 550 824 901 262 1553
Fragments arranged by size:
1553 1004 926 901 824 550 262 197
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6217
SphI G_CATG'C
Cuts at: 1132 3575 1132
Size: 2443 3774
SspI AAT'ATT
Cuts at: 5 52 4141 5
Size: 47 4089 2081
Fragments arranged by size:
4089 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6217
StyI C'CwwG_G
Cuts at: 513 1065 2622 2934 3656 513
Size: 552 1557 312 722 3074
Fragments arranged by size:
3074 1557 722 552 312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2024 2930 4244 4261 4414 4599 5044
Size: 1255 444 906 1314 17 153 185 445
Cuts at: 5044 325
Size: 1498
Fragments arranged by size:
1498 1314 1255 906 445 444 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4463 6175 96
Size: 274 80 33 51 1443 2486 1712 138
Fragments arranged by size:
2486 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2512 2544 6177 6209 2512
Size: 32 3633 32 2520
Fragments arranged by size:
3633 2520 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6217
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3106 3106
Size: 6217
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 2668 3173 3542 1870
Size: 798 505 369 4545
Fragments arranged by size:
4545 798 505 369
XmnI GAAnn'nnTTC
Cuts at: 4346 4346
Size: 6217
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2827 3318 4025 276
Size: 53 83 186 936 1293 491 707 2468
Fragments arranged by size:
2468 1293 936 707 491 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
KasI NarI NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SapI SfiI SfoI SrfI SwaI