Restriction map for
pFN21AE2740
(Linear) (Six-base) MAPSORT of: KIEE2740.seq Check: 7263 from: 1 to: 1512
KIEE2740.orf
With 182 enzymes: SgfI *
December 17, 2018 10:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1168 1512
Size: 1168 344
AatII G_ACGT'C
Cuts at: 0 824 1315 1512
Size: 824 491 197
Fragments arranged by size:
824 491 197
AccI GT'mk_AC
Cuts at: 0 119 1512
Size: 119 1393
AflIII A'CryG_T
Cuts at: 0 1082 1512
Size: 1082 430
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1180 1512
Size: 1180 332
AlwNI CAG_nnn'CTG
Cuts at: 0 907 1512
Size: 907 605
ApaI G_GGCC'C
Cuts at: 0 625 1140 1209 1512
Size: 625 515 69 303
Fragments arranged by size:
625 515 303 69
ApaLI G'TGCA_C
Cuts at: 0 1155 1512
Size: 1155 357
AvaI C'yCGr_G
Cuts at: 0 663 1141 1168 1512
Size: 663 478 27 344
Fragments arranged by size:
663 478 344 27
BamHI G'GATC_C
Cuts at: 0 308 1512
Size: 308 1204
BanI G'GyrC_C
Cuts at: 0 361 937 1188 1512
Size: 361 576 251 324
Fragments arranged by size:
576 361 324 251
BanII G_rGCy'C
Cuts at: 0 200 625 1003 1111 1140 1148 1209 1421
Size: 200 425 378 108 29 8 61 212
Cuts at: 1421 1512
Size: 91
Fragments arranged by size:
425 378 212 200 108 91 61 29
8
BbsI GAAGACnn'nnnn_
Cuts at: 0 534 1512
Size: 534 978
BlpI GC'TnA_GC
Cuts at: 0 733 1117 1512
Size: 733 384 395
Fragments arranged by size:
733 395 384
Bme1580I G_kGCm'C
Cuts at: 0 625 1140 1159 1193 1209 1345 1512
Size: 625 515 19 34 16 136 167
Fragments arranged by size:
625 515 167 136 34 19 16
BmgBI CAC'GTC
Cuts at: 0 881 1083 1512
Size: 881 202 429
Fragments arranged by size:
881 429 202
BmrI ACTGGGnnnn_n'
Cuts at: 0 540 1114 1512
Size: 540 574 398
Fragments arranged by size:
574 540 398
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 879 1512
Size: 879 633
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 341 742 1029 1512
Size: 214 127 401 287 483
Fragments arranged by size:
483 401 287 214 127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 927 1477 1512
Size: 927 550 35
Fragments arranged by size:
927 550 35
BsaI GGTCTCn'nnnn_
Cuts at: 0 1241 1512
Size: 1241 271
BsaHI Gr'CG_yC
Cuts at: 0 539 821 1312 1512
Size: 539 282 491 200
Fragments arranged by size:
539 491 282 200
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 339 369 1512
Size: 339 30 1143
Fragments arranged by size:
1143 339 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 697 730 993 1026 1512
Size: 697 33 263 33 486
Fragments arranged by size:
697 486 263 33 33
BseYI C'CCAG_C
Cuts at: 0 264 1147 1338 1512
Size: 264 883 191 174
Fragments arranged by size:
883 264 191 174
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 1512
Size: 376 1136
BsiEI CG_ry'CG
Cuts at: 0 560 640 1512
Size: 560 80 872
Fragments arranged by size:
872 560 80
BsiHKAI G_wGCw'C
Cuts at: 0 1003 1159 1512
Size: 1003 156 353
Fragments arranged by size:
1003 353 156
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1341 1512
Size: 1341 171
Bsp1286I G_dGCh'C
Cuts at: 0 200 625 1003 1111 1140 1148 1159 1193
Size: 200 425 378 108 29 8 11 34
Cuts at: 1193 1209 1345 1421 1512
Size: 16 136 76 91
Fragments arranged by size:
425 378 200 136 108 91 76 34
29 16 11 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1168 1512
Size: 1168 344
BsrBI CCG'CTC
Cuts at: 0 377 1512
Size: 377 1135
BsrDI GCAATG_nn'
Cuts at: 0 408 1512
Size: 408 1104
BsrFI r'CCGG_y
Cuts at: 0 639 836 1512
Size: 639 197 676
Fragments arranged by size:
676 639 197
BssSI C'ACGA_G
Cuts at: 0 280 1344 1512
Size: 280 1064 168
Fragments arranged by size:
1064 280 168
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1232 1512
Size: 1232 280
BstXI CCAn_nnnn'nTGG
Cuts at: 0 264 816 1512
Size: 264 552 696
Fragments arranged by size:
696 552 264
BstYI r'GATC_y
Cuts at: 0 308 1512
Size: 308 1204
BtgI C'CryG_G
Cuts at: 0 92 929 970 1512
Size: 92 837 41 542
Fragments arranged by size:
837 542 92 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1002 1512
Size: 1002 510
BtsI GCAGTG_nn'
Cuts at: 0 457 771 1111 1393 1512
Size: 457 314 340 282 119
Fragments arranged by size:
457 340 314 282 119
DrdI GACnn_nn'nnGTC
Cuts at: 0 595 1512
Size: 595 917
EaeI y'GGCC_r
Cuts at: 0 233 419 557 637 850 990 1289 1305
Size: 233 186 138 80 213 140 299 16
Cuts at: 1305 1512
Size: 207
Fragments arranged by size:
299 233 213 207 186 140 138 80
16
EagI C'GGCC_G
Cuts at: 0 557 637 1512
Size: 557 80 875
Fragments arranged by size:
875 557 80
EarI CTCTTCn'nnn_
Cuts at: 0 52 1512
Size: 52 1460
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1305 1512
Size: 1305 207
EcoICRI GAG'CTC
Cuts at: 0 1001 1512
Size: 1001 511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 341 742 1029 1512
Size: 214 127 401 287 483
Fragments arranged by size:
483 401 287 214 127
EcoNI CCTnn'n_nnAGG
Cuts at: 0 250 1512
Size: 250 1262
EcoO109I rG'GnC_Cy
Cuts at: 0 147 216 621 622 667 892 1136 1137
Size: 147 69 405 1 45 225 244 1
Cuts at: 1137 1356 1371 1512
Size: 219 15 141
Fragments arranged by size:
405 244 225 219 147 141 69 45
15 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 642 1512
Size: 642 870
HaeII r_GCGC'y
Cuts at: 0 16 477 830 1512
Size: 16 461 353 682
Fragments arranged by size:
682 461 353 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 339 371 1512
Size: 339 32 1141
Fragments arranged by size:
1141 339 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 350 1512
Size: 113 237 1162
Fragments arranged by size:
1162 237 113
MscI TGG'CCA
Cuts at: 0 235 421 852 1512
Size: 235 186 431 660
Fragments arranged by size:
660 431 235 186
MspA1I CmG'CkG
Cuts at: 0 720 907 963 972 978 1013 1132 1512
Size: 720 187 56 9 6 35 119 380
Fragments arranged by size:
720 380 187 119 56 35 9 6
NaeI GCC'GGC
Cuts at: 0 838 1512
Size: 838 674
NcoI C'CATG_G
Cuts at: 0 929 1512
Size: 929 583
NgoMIV G'CCGG_C
Cuts at: 0 836 1512
Size: 836 676
NspI r_CATG'y
Cuts at: 0 751 1475 1512
Size: 751 724 37
Fragments arranged by size:
751 724 37
PasI CC'CwG_GG
Cuts at: 0 1377 1421 1512
Size: 1377 44 91
Fragments arranged by size:
1377 91 44
PflMI CCAn_nnn'nTGG
Cuts at: 0 231 1483 1512
Size: 231 1252 29
Fragments arranged by size:
1252 231 29
PpuMI rG'GwC_Cy
Cuts at: 0 667 1512
Size: 667 845
PshAI GACnn'nnGTC
Cuts at: 0 656 1512
Size: 656 856
PspOMI G'GGCC_C
Cuts at: 0 621 1136 1205 1512
Size: 621 515 69 307
Fragments arranged by size:
621 515 307 69
PstI C_TGCA'G
Cuts at: 0 983 1512
Size: 983 529
PvuII CAG'CTG
Cuts at: 0 720 907 978 1512
Size: 720 187 71 534
Fragments arranged by size:
720 534 187 71
SacI G_AGCT'C
Cuts at: 0 1003 1512
Size: 1003 509
SacII CC_GC'GG
Cuts at: 0 973 1512
Size: 973 539
SanDI GG'GwC_CC
Cuts at: 0 667 1512
Size: 667 845
SexAI A'CCwGG_T
Cuts at: 0 517 1184 1512
Size: 517 667 328
Fragments arranged by size:
667 517 328
SfcI C'TryA_G
Cuts at: 0 715 979 1512
Size: 715 264 533
Fragments arranged by size:
715 533 264
SmaI CCC'GGG
Cuts at: 0 665 1170 1512
Size: 665 505 342
Fragments arranged by size:
665 505 342
SmlI C'TyrA_G
Cuts at: 0 942 1492 1512
Size: 942 550 20
Fragments arranged by size:
942 550 20
StyI C'CwwG_G
Cuts at: 0 617 929 1512
Size: 617 312 583
Fragments arranged by size:
617 583 312
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 925 1512
Size: 19 906 587
Fragments arranged by size:
906 587 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 507 539 1512
Size: 507 32 973
Fragments arranged by size:
973 507 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1101 1512
Size: 1101 411
XmaI C'CCGG_G
Cuts at: 0 663 1168 1512
Size: 663 505 344
Fragments arranged by size:
663 505 344
ZraI GAC'GTC
Cuts at: 0 822 1313 1512
Size: 822 491 199
Fragments arranged by size:
822 491 199
Enzymes that do cut:
AarI AatII AccI AflIII AlfI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbsI BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstXI BstYI BtgI BtgZI
BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I MmeI MscI MspA1I
NaeI NcoI NgoMIV NspI PasI PflMI PpuMI PshAI
PspOMI PstI PvuII SacI SacII SanDI SexAI SfcI
SmaI SmlI StyI TaqII TstI XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI
AloI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BmtI
Bpu10I BsaAI BsaBI BsaWI BsiWI BsmI BspEI BspHI
BsrGI BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DraI DraIII EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MslI NarI
NdeI NheI NotI NruI NsiI PacI PciI PfoI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII
SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XbaI XhoI XmnI