################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AgeI Alw44I ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BlnI BsaBI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI DsaI EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MroI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RcaI RsrII SacII SalI ScaI SfiI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AatII 421 GACGTC GACGT^C AfaI 69 GTAC GT^AC AflIII 488 ACATGT A^C[GA][CT]GT AlwI 747 GATCC GGATC(4/5) AocI 1260 CCTCAGG CC^T[ACGT]AGG Aor51HI 683 AGCGCT AGC^GCT AspHI 1255 GAGCTC G[AT]GC[AT]^C AspI 1134 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 726 TGCGCA TGC^GCA BbeI 250 GGCGCC GGCGC^C BbsI 661 GAAGAC GAAGAC(2/6) BglII 779 AGATCT A^GATCT BlpI 333 GCTGAGC GC^T[ACGT]AGC Bpu1102I 333 GCTGAGC GC^T[ACGT]AGC BpuAI 661 GAAGAC GAAGAC(2/6) BsaAI 709 TACGTG [CT]AC^GT[GA] BsaI 448 GAGACC GGTCTC(1/5) BsiHKAI 1255 GAGCTC G[AT]GC[AT]^C BspLU11I 488 ACATGT A^CATGT BspMI 207 GCAGGT ACCTGC(4/8) BsrFI 1045 GCCGGC [GA]^CCGG[CT] BstAPI 324 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1260 CCTCAGG CC^T[ACGT]AGG CelII 333 GCTGAGC GC^T[ACGT]AGC Cfr10I 1045 GCCGGC [GA]^CCGG[CT] DraIII 1006 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1047 CGGCCG C^GGCCG EclXI 1047 CGGCCG C^GGCCG Eco47III 683 AGCGCT AGC^GCT Eco52I 1047 CGGCCG C^GGCCG Eco81I 1260 CCTCAGG CC^T[ACGT]AGG EcoT14I 437 CCTTGG C^C[AT][AT]GG FspI 726 TGCGCA TGC^GCA HphI 648 GGTGA GGTGA(8/7) KasI 250 GGCGCC G^GCGCC MseI 637 TTAA T^TAA MslI 574 CACACAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 1045 GCCGGC GCC^GGC NarI 250 GGCGCC GG^CGCC NgoMIV 1045 GCCGGC G^CCGGC PaeR7I 1031 CTCGAG C^TCGAG PflFI 1134 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 651 GACTC GAGTC(4/5) PvuI 945 CGATCG CGAT^CG RsaI 69 GTAC GT^AC SacI 1255 GAGCTC GAGCT^C SapI 1252 GAAGAGC GCTCTTC(1/4) SbfI 99 CCTGCAGG CCTGCA^GG SexAI 1007 ACCAGGT A^CC[AT]GGT SfoI 250 GGCGCC GGC^GCC SmlI 1031 CTCGAG C^T[CT][GA]AG SphI 528 GCATGC GCATG^C Sse8387I 99 CCTGCAGG CCTGCA^GG StuI 1126 AGGCCT AGG^CCT StyI 437 CCTTGG C^C[AT][AT]GG Tru9I 637 TTAA T^TAA Tsp509I 279 AATT ^AATT Tth111I 1134 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1031 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AhdI 10 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 925 GACTTCATGTC AspEI 10 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 925 GACTTCATGTC BalI 440 TGGCCA TGG^CCA 503 TGGCCA BanI 250 GGCGCC G^G[CT][GA]CC 1110 GGTGCC BfaI 187 CTAG C^TAG 301 CTAG BglI 148 GCCCGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 253 GCCGCCGAGGC BsiEI 945 CGATCG CG[GA][CT]^CG 1047 CGGCCG BsmAI 448 GAGAC GTCTC(1/5) 1020 GAGAC BsrBI 432 CCGCTC CCGCTC(-3/-3) 880 GAGCGG BstXI 506 CCAAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1076 CCAAGTATTTGG BstYI 746 AGATCC [GA]^GATC[CT] 779 AGATCT Eam1105I 10 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 925 GACTTCATGTC EarI 805 GAAGAG CTCTTC(1/4) 1252 GAAGAG HaeII 250 GGCGCC [GA]GCGC^[CT] 683 AGCGCT Ksp632I 805 GAAGAG CTCTTC(1/4) 1252 GAAGAG MaeI 187 CTAG C^TAG 301 CTAG MflI 746 AGATCC [GA]^GATC[CT] 779 AGATCT MluNI 440 TGGCCA TGG^CCA 503 TGGCCA MscI 440 TGGCCA TGG^CCA 503 TGGCCA TaqI 630 TCGA T^CGA 1032 TCGA TthHB8I 630 TCGA T^CGA 1032 TCGA XhoII 746 AGATCC [GA]^GATC[CT] 779 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AccII AciI AcyI AluI AlwNI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssHII BssKI BssSI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PpuMI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SmaI TfiI TseI Tsp45I TspRI XmaCI XmaI