(Circular) (Six-base) MAPSORT of: pFN21AE1902.seq Check: 3114 from: 1 to: 5992 pFN21AE1902 5992 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2430 3802 278 Size: 53 83 186 936 894 1372 2468 Fragments arranged by size: 2468 1372 936 894 186 83 53 AccI GT'mk_AC Cuts at: 1937 3335 1937 Size: 1398 4594 Acc65I G'GTAC_C Cuts at: 3313 3313 Size: 5992 AclI AA'CG_TT Cuts at: 1563 4119 4492 1563 Size: 2556 373 3063 Fragments arranged by size: 3063 2556 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3197 3223 4055 5439 5707 1632 Size: 1565 26 832 1384 268 1917 Fragments arranged by size: 1917 1565 1384 832 268 26 AfeI AGC'GCT Cuts at: 2690 2690 Size: 5992 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5795 AflIII A'CryG_T Cuts at: 1203 2493 4891 5868 1203 Size: 1290 2398 977 1327 Fragments arranged by size: 2398 1327 1290 977 AgeI A'CCGG_T Cuts at: 4823 4823 Size: 5992 AhdI GACnn_n'nnGTC Cuts at: 2020 2935 4721 2020 Size: 915 1786 3291 Fragments arranged by size: 3291 1786 915 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5992 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5992 AlwNI CAG_nnn'CTG Cuts at: 2095 2335 2338 2506 2848 2899 3735 5307 2095 Size: 240 3 168 342 51 836 1572 2780 Fragments arranged by size: 2780 1572 836 342 240 168 51 3 ApaLI G'TGCA_C Cuts at: 4049 5205 4049 Size: 1156 4836 ApoI r'AATT_y Cuts at: 1493 3301 3527 3679 5788 1493 Size: 1808 226 152 2109 1697 Fragments arranged by size: 2109 1808 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4546 160 Size: 872 3514 1606 Fragments arranged by size: 3514 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 AvaI C'yCGr_G Cuts at: 1870 1943 2010 2017 2412 2765 2868 3036 3317 Size: 73 67 7 395 353 103 168 281 Cuts at: 3317 1870 Size: 4545 Fragments arranged by size: 4545 395 353 281 168 103 73 67 7 BamHI G'GATC_C Cuts at: 3322 3689 3322 Size: 367 5625 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2255 3115 3313 4768 618 Size: 325 217 627 468 860 198 1455 1842 Fragments arranged by size: 1842 1455 860 627 468 325 217 198 BanII G_rGCy'C Cuts at: 1391 2727 2766 2965 3264 3311 1391 Size: 1336 39 199 299 47 4072 Fragments arranged by size: 4072 1336 299 199 47 39 BbeI G_GCGC'C Cuts at: 2259 2259 Size: 5992 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2673 928 Size: 971 774 4247 Fragments arranged by size: 4247 971 774 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4183 4217 1903 Size: 34 2246 34 3678 Fragments arranged by size: 3678 2246 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3884 5094 3884 Size: 1210 4782 BclI T'GATC_A Cuts at: 1548 1548 Size: 5992 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2159 2264 4603 Size: 107 122 71 631 605 487 105 2339 Cuts at: 4603 136 Size: 1525 Fragments arranged by size: 2339 1525 631 605 487 122 107 105 71 BglII A'GATC_T Cuts at: 2784 5987 2784 Size: 3203 2789 BlpI GC'TnA_GC Cuts at: 2339 3409 2339 Size: 1070 4922 Bme1580I G_kGCm'C Cuts at: 1790 2592 3221 4053 5209 1790 Size: 802 629 832 1156 2573 Fragments arranged by size: 2573 1156 832 802 629 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5992 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3150 3723 4681 Size: 416 53 83 399 24 1733 573 958 Cuts at: 4681 442 Size: 1753 Fragments arranged by size: 1753 1733 958 573 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5992 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 2228 2842 3005 3043 3148 4652 1239 Size: 950 39 614 163 38 105 1504 2579 Fragments arranged by size: 2579 1504 950 614 163 105 39 38 Bpu10I CC'TnA_GC Cuts at: 2738 2738 Size: 5992 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4117 4982 5280 4117 Size: 865 298 4829 Fragments arranged by size: 4829 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2447 4655 882 Size: 617 948 2208 2219 Fragments arranged by size: 2219 2208 948 617 BsaAI yAC'GTr Cuts at: 493 1204 2716 3783 493 Size: 711 1512 1067 2702 Fragments arranged by size: 2702 1512 1067 711 BsaBI GATnn'nnATC Cuts at: 5986 5986 Size: 5992 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2130 2178 2256 2427 Size: 53 83 186 936 597 48 78 171 Cuts at: 2427 3048 3799 4181 4816 275 Size: 621 751 382 635 1451 Fragments arranged by size: 1451 936 751 635 621 597 382 186 171 83 78 53 48 BsaWI w'CCGG_w Cuts at: 4425 4823 5097 5244 5655 4425 Size: 398 274 147 411 4762 Fragments arranged by size: 4762 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5767 Fragments arranged by size: 5767 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2401 3254 1186 Size: 865 350 853 3924 Fragments arranged by size: 3924 865 853 350 BseYI C'CCAG_C Cuts at: 2031 3065 3147 5195 5691 2031 Size: 1034 82 2048 496 2332 Fragments arranged by size: 2332 2048 1034 496 82 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2050 2912 2993 1163 Size: 573 314 862 81 4162 Fragments arranged by size: 4162 862 573 314 81 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2953 3055 3442 4203 5231 1540 Size: 275 186 952 102 387 761 1028 2301 Fragments arranged by size: 2301 1028 952 761 387 275 186 102 BsiHKAI G_wGCw'C Cuts at: 3264 4053 4138 5209 3264 Size: 789 85 1071 4047 Fragments arranged by size: 4047 1071 789 85 BsmI GAATG_Cn' Cuts at: 3503 3596 3503 Size: 93 5899 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2592 2727 2766 2965 3221 3264 3311 Size: 399 802 135 39 199 256 43 47 Cuts at: 3311 4053 4138 5209 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 802 742 399 256 199 135 85 47 43 39 BspEI T'CCGG_A Cuts at: 5655 5655 Size: 5992 BspHI T'CATG_A Cuts at: 3881 5611 3881 Size: 1730 4262 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2203 3347 844 Size: 1359 1144 3489 Fragments arranged by size: 3489 1359 1144 BsrBI CCG'CTC Cuts at: 1394 1823 2439 2887 3879 1394 Size: 429 616 448 992 3507 Fragments arranged by size: 3507 992 616 448 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4487 4661 65 Size: 1183 3239 174 1396 Fragments arranged by size: 3239 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3050 4636 4823 1700 Size: 110 1240 1586 187 2869 Fragments arranged by size: 2869 1586 1240 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5992 BssHII G'CGCG_C Cuts at: 1925 2047 2219 2407 1925 Size: 122 172 188 5510 Fragments arranged by size: 5510 188 172 122 BssSI C'ACGA_G Cuts at: 2078 2354 2759 4052 5064 2078 Size: 276 405 1293 1012 3006 Fragments arranged by size: 3006 1293 1012 405 276 BstAPI GCAn_nnn'nTGC Cuts at: 794 2335 794 Size: 1541 4451 BstBI TT'CG_AA Cuts at: 4796 4796 Size: 5992 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5992 BstXI CCAn_nnnn'nTGG Cuts at: 2518 3088 2518 Size: 570 5422 BstYI r'GATC_y Cuts at: 1972 2751 2784 3322 3689 4074 4091 5543 5629 Size: 779 33 538 367 385 17 1452 86 Cuts at: 5629 5641 5987 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 779 538 385 367 346 86 33 17 12 Bsu36I CC'TnA_GG Cuts at: 3266 3266 Size: 5992 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5440 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2122 487 Size: 683 56 896 4357 Fragments arranged by size: 4357 896 683 56 BtsI GCAGTG_nn' Cuts at: 3223 3239 3508 4302 4322 3223 Size: 16 269 794 20 4893 Fragments arranged by size: 4893 794 269 20 16 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5992 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5957 DraI TTT'AAA Cuts at: 3296 3296 Size: 5992 DraIII CAC_nnn'GTG Cuts at: 3016 3016 Size: 5992 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4999 809 Size: 507 3683 1802 Fragments arranged by size: 3683 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2160 2445 2508 Size: 54 1425 50 275 18 330 285 63 Cuts at: 2508 3052 3090 3439 4328 5957 8 Size: 544 38 349 889 1629 43 Fragments arranged by size: 1629 1425 889 544 349 330 285 275 63 54 50 43 38 18 EagI C'GGCC_G Cuts at: 1537 1812 3052 3439 1537 Size: 275 1240 387 4090 Fragments arranged by size: 4090 1240 387 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2805 3252 3922 1353 Size: 1452 447 670 3423 Fragments arranged by size: 3423 1452 670 447 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4579 4953 5099 387 Size: 1064 138 2990 374 146 1280 Fragments arranged by size: 2990 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3262 3262 Size: 5992 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2228 2842 3005 3043 3148 3197 Size: 393 557 39 614 163 38 105 49 Cuts at: 3197 3223 4055 4652 5439 5707 1239 Size: 26 832 597 787 268 1524 Fragments arranged by size: 1524 832 787 614 597 557 393 268 163 105 49 39 38 26 EcoO109I rG'GnC_Cy Cuts at: 2014 2041 3144 3208 2014 Size: 27 1103 64 4798 Fragments arranged by size: 4798 1103 64 27 EcoRI G'AATT_C Cuts at: 3301 3679 3301 Size: 378 5614 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2947 2947 Size: 5992 FspI TGC'GCA Cuts at: 2733 4498 2733 Size: 1765 4227 HaeII r_GCGC'y Cuts at: 1678 2259 2692 5139 1678 Size: 581 433 2447 2531 Fragments arranged by size: 2531 2447 581 433 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2006 2038 2921 2953 4633 Size: 32 135 32 434 32 883 32 1680 Cuts at: 4633 4665 4707 4739 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1680 883 434 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3336 3582 669 Size: 988 281 1398 246 3079 Fragments arranged by size: 3079 1398 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5992 HpaI GTT'AAC Cuts at: 3582 3582 Size: 5992 KasI G'GCGC_C Cuts at: 2255 2255 Size: 5992 KpnI G_GTAC'C Cuts at: 3317 3317 Size: 5992 MfeI C'AATT_G Cuts at: 3591 3591 Size: 5992 MluI A'CGCG_T Cuts at: 5868 5868 Size: 5992 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2553 2787 2862 5106 5290 1305 Size: 1248 234 75 2244 184 2007 Fragments arranged by size: 2244 2007 1248 234 184 75 MscI TGG'CCA Cuts at: 10 64 1489 1832 2447 2510 10 Size: 54 1425 343 615 63 3492 Fragments arranged by size: 3492 1425 615 343 63 54 MslI CAynn'nnrTG Cuts at: 518 1166 2583 3950 4309 4468 518 Size: 648 1417 1367 359 159 2042 Fragments arranged by size: 2042 1417 1367 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2054 2092 2236 2335 2464 3408 4085 5233 Size: 448 38 144 99 129 944 677 1148 Cuts at: 5233 5478 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 944 677 448 245 144 129 99 38 NaeI GCC'GGC Cuts at: 3052 3052 Size: 5992 NarI GG'CG_CC Cuts at: 2256 2256 Size: 5992 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5440 NdeI CA'TA_TG Cuts at: 387 387 Size: 5992 NgoMIV G'CCGG_C Cuts at: 3050 3050 Size: 5992 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5992 NotI GC'GGCC_GC Cuts at: 3439 3439 Size: 5992 NspI r_CATG'y Cuts at: 1132 1229 2497 2537 3275 3350 4895 1132 Size: 97 1268 40 738 75 1545 2229 Fragments arranged by size: 2229 1545 1268 738 97 75 40 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5992 PciI A'CATG_T Cuts at: 2493 4891 2493 Size: 2398 3594 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5992 PfoI T'CCnGG_A Cuts at: 2226 2858 3020 2226 Size: 632 162 5198 Fragments arranged by size: 5198 632 162 PmeI GTTT'AAAC Cuts at: 3296 3296 Size: 5992 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4061 4093 5574 5606 4061 Size: 32 1481 32 4447 Fragments arranged by size: 4447 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2014 3144 3208 2014 Size: 1130 64 4798 Fragments arranged by size: 4798 1130 64 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5992 PsiI TTA'TAA Cuts at: 3562 3562 Size: 5992 PstI C_TGCA'G Cuts at: 830 2109 2751 3169 3234 3344 830 Size: 1279 642 418 65 110 3478 Fragments arranged by size: 3478 1279 642 418 110 65 PvuI CG_AT'CG Cuts at: 2001 2953 2001 Size: 952 5040 PvuII CAG'CTG Cuts at: 2054 2236 2335 2464 2054 Size: 182 99 129 5582 Fragments arranged by size: 5582 182 129 99 SacI G_AGCT'C Cuts at: 3264 3264 Size: 5992 SalI G'TCGA_C Cuts at: 1936 3334 1936 Size: 1398 4594 SanDI GG'GwC_CC Cuts at: 2014 3144 2014 Size: 1130 4862 SapI GCTCTTCn'nnn_ Cuts at: 3252 3252 Size: 5992 SbfI CC_TGCA'GG Cuts at: 2109 3344 2109 Size: 1235 4757 ScaI AGT'ACT Cuts at: 4240 5952 4240 Size: 1712 4280 SexAI A'CCwGG_T Cuts at: 3012 3012 Size: 5992 SfcI C'TryA_G Cuts at: 826 1045 2105 2747 3165 3230 3340 4475 5156 Size: 219 1060 642 418 65 110 1135 681 Cuts at: 5156 5347 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1060 681 642 418 219 191 110 65 SfoI GGC'GCC Cuts at: 2257 2257 Size: 5992 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5992 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5992 SmaI CCC'GGG Cuts at: 1872 2012 2019 2414 2870 3319 1872 Size: 140 7 395 456 449 4545 Fragments arranged by size: 4545 456 449 395 140 7 SmlI C'TyrA_G Cuts at: 820 1017 1943 3036 4096 4997 5259 820 Size: 197 926 1093 1060 901 262 1553 Fragments arranged by size: 1553 1093 1060 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3783 493 Size: 3290 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5992 SphI G_CATG'C Cuts at: 1132 2537 3350 1132 Size: 1405 813 3774 Fragments arranged by size: 3774 1405 813 SrfI GCCC'GGGC Cuts at: 2414 2414 Size: 5992 SspI AAT'ATT Cuts at: 5 52 3916 5 Size: 47 3864 2081 Fragments arranged by size: 3864 2081 47 StuI AGG'CCT Cuts at: 1517 3133 1517 Size: 1616 4376 StyI C'CwwG_G Cuts at: 513 1065 2442 3431 513 Size: 552 1377 989 3074 Fragments arranged by size: 3074 1377 989 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4019 4036 4189 4374 4819 325 Size: 1255 2439 17 153 185 445 1498 Fragments arranged by size: 2439 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4238 5950 96 Size: 274 80 33 51 1443 2261 1712 138 Fragments arranged by size: 2261 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3002 3034 3200 3232 5952 5984 3002 Size: 32 166 32 2720 32 3010 Fragments arranged by size: 3010 2720 166 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3142 1316 Size: 1826 4166 XbaI T'CTAG_A Cuts at: 3328 3328 Size: 5992 XhoI C'TCGA_G Cuts at: 1943 3036 1943 Size: 1093 4899 XmaI C'CCGG_G Cuts at: 1870 2010 2017 2412 2868 3317 1870 Size: 140 7 395 456 449 4545 Fragments arranged by size: 4545 456 449 395 140 7 XmnI GAAnn'nnTTC Cuts at: 4121 4121 Size: 5992 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2428 3800 276 Size: 53 83 186 936 894 1372 2468 Fragments arranged by size: 2468 1372 936 894 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SfiI SwaI XcmI