(Linear) (Six-base) MAPSORT of: KIEE1902.seq Check: 2790 from: 1 to: 1287 KIEE1902.orf With 182 enzymes: SgfI * July 15, 2010 18:50 .. AatII G_ACGT'C Cuts at: 0 425 1287 Size: 425 862 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1192 1218 1287 Size: 1192 26 69 Fragments arranged by size: 1192 69 26 AfeI AGC'GCT Cuts at: 0 685 1287 Size: 685 602 AflIII A'CryG_T Cuts at: 0 488 1287 Size: 488 799 AhdI GACnn_n'nnGTC Cuts at: 0 15 930 1287 Size: 15 915 357 Fragments arranged by size: 915 357 15 AlwNI CAG_nnn'CTG Cuts at: 0 90 330 333 501 843 894 1287 Size: 90 240 3 168 342 51 393 Fragments arranged by size: 393 342 240 168 90 51 3 AvaI C'yCGr_G Cuts at: 0 5 12 407 760 863 1031 1287 Size: 5 7 395 353 103 168 256 Fragments arranged by size: 395 353 256 168 103 7 5 BanI G'GyrC_C Cuts at: 0 250 1110 1287 Size: 250 860 177 Fragments arranged by size: 860 250 177 BanII G_rGCy'C Cuts at: 0 722 761 960 1259 1287 Size: 722 39 199 299 28 Fragments arranged by size: 722 299 199 39 28 BbeI G_GCGC'C Cuts at: 0 254 1287 Size: 254 1033 BbsI GAAGACnn'nnnn_ Cuts at: 0 668 1287 Size: 668 619 BglI GCCn_nnn'nGGC Cuts at: 0 154 259 1287 Size: 154 105 1028 Fragments arranged by size: 1028 154 105 BglII A'GATC_T Cuts at: 0 779 1287 Size: 779 508 BlpI GC'TnA_GC Cuts at: 0 334 1287 Size: 334 953 Bme1580I G_kGCm'C Cuts at: 0 587 1216 1287 Size: 587 629 71 Fragments arranged by size: 629 587 71 BmrI ACTGGGnnnn_n' Cuts at: 0 1145 1287 Size: 1145 142 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 223 837 1000 1038 1143 1287 Size: 184 39 614 163 38 105 144 Fragments arranged by size: 614 184 163 144 105 39 38 Bpu10I CC'TnA_GC Cuts at: 0 733 1287 Size: 733 554 BsaI GGTCTCn'nnnn_ Cuts at: 0 442 1287 Size: 442 845 BsaAI yAC'GTr Cuts at: 0 711 1287 Size: 711 576 BsaHI Gr'CG_yC Cuts at: 0 125 173 251 422 1043 1287 Size: 125 48 78 171 621 244 Fragments arranged by size: 621 244 171 125 78 48 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 46 396 1249 1287 Size: 46 350 853 38 Fragments arranged by size: 853 350 46 38 BseYI C'CCAG_C Cuts at: 0 26 1060 1142 1287 Size: 26 1034 82 145 Fragments arranged by size: 1034 145 82 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 45 907 988 1287 Size: 45 862 81 299 Fragments arranged by size: 862 299 81 45 BsiEI CG_ry'CG Cuts at: 0 948 1050 1287 Size: 948 102 237 Fragments arranged by size: 948 237 102 BsiHKAI G_wGCw'C Cuts at: 0 1259 1287 Size: 1259 28 Bsp1286I G_dGCh'C Cuts at: 0 587 722 761 960 1216 1259 1287 Size: 587 135 39 199 256 43 28 Fragments arranged by size: 587 256 199 135 43 39 28 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 198 1287 Size: 198 1089 BsrBI CCG'CTC Cuts at: 0 434 882 1287 Size: 434 448 405 Fragments arranged by size: 448 434 405 BsrFI r'CCGG_y Cuts at: 0 1045 1287 Size: 1045 242 BssHII G'CGCG_C Cuts at: 0 42 214 402 1287 Size: 42 172 188 885 Fragments arranged by size: 885 188 172 42 BssSI C'ACGA_G Cuts at: 0 73 349 754 1287 Size: 73 276 405 533 Fragments arranged by size: 533 405 276 73 BstAPI GCAn_nnn'nTGC Cuts at: 0 330 1287 Size: 330 957 BstXI CCAn_nnnn'nTGG Cuts at: 0 513 1083 1287 Size: 513 570 204 Fragments arranged by size: 570 513 204 BstYI r'GATC_y Cuts at: 0 746 779 1287 Size: 746 33 508 Fragments arranged by size: 746 508 33 Bsu36I CC'TnA_GG Cuts at: 0 1261 1287 Size: 1261 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 117 1287 Size: 117 1170 BtsI GCAGTG_nn' Cuts at: 0 1218 1234 1287 Size: 1218 16 53 Fragments arranged by size: 1218 53 16 DraIII CAC_nnn'GTG Cuts at: 0 1011 1287 Size: 1011 276 EaeI y'GGCC_r Cuts at: 0 155 440 503 1047 1085 1287 Size: 155 285 63 544 38 202 Fragments arranged by size: 544 285 202 155 63 38 EagI C'GGCC_G Cuts at: 0 1047 1287 Size: 1047 240 EarI CTCTTCn'nnn_ Cuts at: 0 800 1247 1287 Size: 800 447 40 Fragments arranged by size: 800 447 40 EcoICRI GAG'CTC Cuts at: 0 1257 1287 Size: 1257 30 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 184 223 837 1000 1038 1143 1192 1218 Size: 184 39 614 163 38 105 49 26 Cuts at: 1218 1287 Size: 69 Fragments arranged by size: 614 184 163 105 69 49 39 38 26 EcoO109I rG'GnC_Cy Cuts at: 0 9 36 1139 1203 1287 Size: 9 27 1103 64 84 Fragments arranged by size: 1103 84 64 27 9 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 942 1287 Size: 942 345 FspI TGC'GCA Cuts at: 0 728 1287 Size: 728 559 HaeII r_GCGC'y Cuts at: 0 254 687 1287 Size: 254 433 600 Fragments arranged by size: 600 433 254 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1 33 916 948 1287 Size: 1 32 883 32 339 Fragments arranged by size: 883 339 32 32 1 KasI G'GCGC_C Cuts at: 0 250 1287 Size: 250 1037 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 548 782 857 1287 Size: 548 234 75 430 Fragments arranged by size: 548 430 234 75 MscI TGG'CCA Cuts at: 0 442 505 1287 Size: 442 63 782 Fragments arranged by size: 782 442 63 MslI CAynn'nnrTG Cuts at: 0 578 1287 Size: 578 709 MspA1I CmG'CkG Cuts at: 0 49 87 231 330 459 1287 Size: 49 38 144 99 129 828 Fragments arranged by size: 828 144 129 99 49 38 NaeI GCC'GGC Cuts at: 0 1047 1287 Size: 1047 240 NarI GG'CG_CC Cuts at: 0 251 1287 Size: 251 1036 NgoMIV G'CCGG_C Cuts at: 0 1045 1287 Size: 1045 242 NspI r_CATG'y Cuts at: 0 492 532 1270 1287 Size: 492 40 738 17 Fragments arranged by size: 738 492 40 17 PciI A'CATG_T Cuts at: 0 488 1287 Size: 488 799 PfoI T'CCnGG_A Cuts at: 0 221 853 1015 1287 Size: 221 632 162 272 Fragments arranged by size: 632 272 221 162 PpuMI rG'GwC_Cy Cuts at: 0 9 1139 1203 1287 Size: 9 1130 64 84 Fragments arranged by size: 1130 84 64 9 PstI C_TGCA'G Cuts at: 0 104 746 1164 1229 1287 Size: 104 642 418 65 58 Fragments arranged by size: 642 418 104 65 58 PvuI CG_AT'CG Cuts at: 0 948 1287 Size: 948 339 PvuII CAG'CTG Cuts at: 0 49 231 330 459 1287 Size: 49 182 99 129 828 Fragments arranged by size: 828 182 129 99 49 SacI G_AGCT'C Cuts at: 0 1259 1287 Size: 1259 28 SanDI GG'GwC_CC Cuts at: 0 9 1139 1287 Size: 9 1130 148 Fragments arranged by size: 1130 148 9 SapI GCTCTTCn'nnn_ Cuts at: 0 1247 1287 Size: 1247 40 SbfI CC_TGCA'GG Cuts at: 0 104 1287 Size: 104 1183 SexAI A'CCwGG_T Cuts at: 0 1007 1287 Size: 1007 280 SfcI C'TryA_G Cuts at: 0 100 742 1160 1225 1287 Size: 100 642 418 65 62 Fragments arranged by size: 642 418 100 65 62 SfoI GGC'GCC Cuts at: 0 252 1287 Size: 252 1035 SmaI CCC'GGG Cuts at: 0 7 14 409 865 1287 Size: 7 7 395 456 422 Fragments arranged by size: 456 422 395 7 7 SmlI C'TyrA_G Cuts at: 0 1031 1287 Size: 1031 256 SphI G_CATG'C Cuts at: 0 532 1287 Size: 532 755 SrfI GCCC'GGGC Cuts at: 0 409 1287 Size: 409 878 StuI AGG'CCT Cuts at: 0 1128 1287 Size: 1128 159 StyI C'CwwG_G Cuts at: 0 437 1287 Size: 437 850 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 997 1029 1195 1227 1287 Size: 997 32 166 32 60 Fragments arranged by size: 997 166 60 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1137 1287 Size: 1137 150 XhoI C'TCGA_G Cuts at: 0 1031 1287 Size: 1031 256 XmaI C'CCGG_G Cuts at: 0 5 12 407 863 1287 Size: 5 7 395 456 424 Fragments arranged by size: 456 424 395 7 5 ZraI GAC'GTC Cuts at: 0 423 1287 Size: 423 864 Enzymes that do cut: AatII AcuI AfeI AflIII AhdI AlwNI AvaI BanI BanII BbeI BbsI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgZI BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI PpuMI PstI PvuI PvuII SacI SanDI SapI SbfI SexAI SfcI SfoI SmaI SmlI SphI SrfI StuI StyI TstI Tth111I XhoI XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AflII AgeI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI BplI BpuEI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BstBI BstEII BstZ17I BtgI ClaI CspCI DraI DrdI EciI EcoNI EcoRI EcoRV FseI FspAI HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI RsrII SacII SalI ScaI SfiI SgfI SgrAI SnaBI SpeI SspI SwaI TaqII TatI XbaI XcmI XmnI