################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI AspHI AspI AvaI AviII AvrII BalI BanII BbrPI BbsI BbvCI BciVI BclI BfrI BglI BglII BlnI BmyI BpuAI BsaAI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DrdI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI Ksp632I MaeII MfeI MluI MluNI MroI MscI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SalI SapI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StyI SwaI TspRI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AhdI 90 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 114 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 555 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 90 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 636 GGACC G^G[AT]CC BamHI 518 GGATCC G^GATCC BbeI 684 GGCGCC GGCGC^C BcgI 539 CGACCGTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 313 CCGGG CC^[GC]GG BfaI 665 CTAG C^TAG BlpI 527 GCTCAGC GC^T[ACGT]AGC Bpu1102I 527 GCTCAGC GC^T[ACGT]AGC BsaI 331 GAGACC GGTCTC(1/5) BsiEI 539 CGACCG CG[GA][CT]^CG BsmFI 26 GGGAC GGGAC(10/14) BspHI 207 TCATGA T^CATGA BsrBI 688 CCGCTC CCGCTC(-3/-3) BstAPI 113 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 87 GGTGACC G^GT[ACGT]ACC BstF5I 587 CATCC GGATG(2/0) BstPI 87 GGTGACC G^GT[ACGT]ACC BstXI 363 CCATCGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 500 GCGGGC GC[ACGT]^[ACGT]GC CelII 527 GCTCAGC GC^T[ACGT]AGC Cfr13I 636 GGACC G^G[ACGT]CC DraIII 663 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 90 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 87 GGTGACC G^GT[ACGT]ACC FokI 587 CATCC GGATG(9/13) HaeII 684 GGCGCC [GA]GCGC^[CT] HgaI 76 GACGC GACGC(5/10) HincII 3 GTTGAC GT[CT]^[GA]AC HindII 3 GTTGAC GT[CT]^[GA]AC KasI 684 GGCGCC G^GCGCC KspI 410 CCGCGG CCGC^GG MaeI 665 CTAG C^TAG MseI 593 TTAA T^TAA NarI 684 GGCGCC GG^CGCC NciI 313 CCGGG CC^[GC]GG RcaI 207 TCATGA T^CATGA SacII 410 CCGCGG CCGC^GG Sau96I 636 GGACC G^G[ACGT]CC SbfI 161 CCTGCAGG CCTGCA^GG SfoI 684 GGCGCC GGC^GCC SmlI 646 CTCAAG C^T[CT][GA]AG Sse8387I 161 CCTGCAGG CCTGCA^GG StuI 249 AGGCCT AGG^CCT TaqI 74 TCGA T^CGA Tru9I 593 TTAA T^TAA TthHB8I 74 TCGA T^CGA XmnI 555 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### Acc65I 65 GGTACC G^GTACC 243 GGTACC AccII 411 CGCG CG^CG 499 CGCG AcyI 76 GACGCC G[GA]^CG[CT]C 684 GGCGCC Asp718I 65 GGTACC G^GTACC 243 GGTACC BpmI 136 CTGGAG CTGGAG(16/14) 475 CTGGAG BsaBI 168 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 358 GATTCCCATC BsaHI 76 GACGCC G[GA]^CG[CT]C 684 GGCGCC BsmAI 317 GAGAC GTCTC(1/5) 331 GAGAC BstUI 411 CGCG CG^CG 499 CGCG BstYI 518 GGATCC [GA]^GATC[CT] 623 AGATCC CfoI 498 GCGC GCG^C 685 GCGC DdeI 354 CTTAG C^T[ACGT]AG 528 CTCAG DsaI 83 CCACGG C^C[GA][CT]GG 410 CCGCGG EcoNI 107 CCTTCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 627 CCTGGGCAAGG FauI 62 GCGGG CCCGC(4/6) 500 GCGGG HaeIII 250 GGCC GG^CC 408 GGCC HapII 313 CCGG C^CGG 424 CCGG HhaI 498 GCGC GCG^C 685 GCGC Hin1I 76 GACGCC G[GA]^CG[CT]C 684 GGCGCC HinP1I 498 GCGC G^CGC 685 GCGC HinfI 358 GATTC G^A[ACGT]TC 674 GATTC HpaII 313 CCGG C^CGG 424 CCGG KpnI 65 GGTACC GGTAC^C 243 GGTACC MamI 168 GATTGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 358 GATTCCCATC MboII 12 GAAGA GAAGA(8/7) 555 GAAGA MflI 518 GGATCC [GA]^GATC[CT] 623 AGATCC MspI 313 CCGG C^CGG 424 CCGG MvnI 411 CGCG CG^CG 499 CGCG SfaNI 467 GCATC GCATC(5/9) 549 GCATC TfiI 358 GATTC G^A[AT]TC 674 GATTC Tsp45I 88 GTGAC ^GT[GC]AC 661 GTCAC Tsp509I 239 AATT ^AATT 296 AATT XhoII 518 GGATCC [GA]^GATC[CT] 623 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AciI AfaI AluI AlwI BanI BbvI BsaJI BseRI BsgI BsiYI BslI BspMI BssKI BstNI DpnII EcoRII Fnu4HI HphI ItaI MaeIII MboI MnlI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PstI RsaI Sau3AI ScrFI SfcI TseI