(Circular) (Six-base) MAPSORT of: pFN21AE4031.seq Check: 8273 from: 1 to: 5431 pFN21AE4031 5431 bp With 182 enzymes: SgfI * March 28, 2012 13:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2060 2165 2174 2455 2060 Size: 105 9 281 5036 Fragments arranged by size: 5036 281 105 9 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3241 278 Size: 53 83 186 936 1705 2468 Fragments arranged by size: 2468 1705 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2774 1937 Size: 837 4594 Acc65I G'GTAC_C Cuts at: 2070 2248 2752 2070 Size: 178 504 4749 Fragments arranged by size: 4749 504 178 AclI AA'CG_TT Cuts at: 1563 3558 3931 1563 Size: 1995 373 3063 Fragments arranged by size: 3063 1995 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2531 2579 3494 4878 5146 1632 Size: 899 48 915 1384 268 1917 Fragments arranged by size: 1917 1384 915 899 268 48 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5234 AflIII A'CryG_T Cuts at: 1203 4330 5307 1203 Size: 3127 977 1327 Fragments arranged by size: 3127 1327 977 AgeI A'CCGG_T Cuts at: 4262 4262 Size: 5431 AhdI GACnn_n'nnGTC Cuts at: 2100 4160 2100 Size: 2060 3371 AleI CACnn'nnGTG Cuts at: 1166 2090 1166 Size: 924 4507 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2330 2516 1184 Size: 1146 186 4099 Fragments arranged by size: 4099 1146 186 AlwNI CAG_nnn'CTG Cuts at: 2124 3174 4746 2124 Size: 1050 1572 2809 Fragments arranged by size: 2809 1572 1050 ApaLI G'TGCA_C Cuts at: 3488 4644 3488 Size: 1156 4275 ApoI r'AATT_y Cuts at: 1493 2740 2966 3118 5227 1493 Size: 1247 226 152 2109 1697 Fragments arranged by size: 2109 1697 1247 226 152 AseI AT'TA_AT Cuts at: 160 1032 3985 160 Size: 872 2953 1606 Fragments arranged by size: 2953 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 AvaI C'yCGr_G Cuts at: 1870 1943 2756 1870 Size: 73 813 4545 Fragments arranged by size: 4545 813 73 BamHI G'GATC_C Cuts at: 2523 2761 3128 2523 Size: 238 367 4826 Fragments arranged by size: 4826 367 238 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2070 2248 2314 2689 2752 Size: 325 217 627 283 178 66 375 63 Cuts at: 2752 4207 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 375 325 283 217 178 66 63 BanII G_rGCy'C Cuts at: 1391 2750 1391 Size: 1359 4072 BbeI G_GCGC'C Cuts at: 2693 2693 Size: 5431 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4460 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2533 2567 3622 3656 1903 Size: 34 596 34 1055 34 3678 Fragments arranged by size: 3678 1055 596 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3323 4533 3323 Size: 1210 4221 BclI T'GATC_A Cuts at: 1548 1548 Size: 5431 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4042 136 Size: 107 122 71 631 605 2370 1525 Fragments arranged by size: 2370 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5426 5426 Size: 5431 BlpI GC'TnA_GC Cuts at: 2533 2848 2533 Size: 315 5116 Bme1580I G_kGCm'C Cuts at: 1790 3492 4648 1790 Size: 1702 1156 2573 Fragments arranged by size: 2573 1702 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5431 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3162 4120 442 Size: 416 53 83 399 24 1745 958 1753 Fragments arranged by size: 1753 1745 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5431 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2359 2359 Size: 5431 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2162 2501 4091 1239 Size: 923 339 1590 2579 Fragments arranged by size: 2579 1590 923 339 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2636 3556 4421 4719 2636 Size: 920 865 298 3348 Fragments arranged by size: 3348 920 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2330 4094 882 Size: 617 831 1764 2219 Fragments arranged by size: 2219 1764 831 617 BsaAI yAC'GTr Cuts at: 493 1204 3222 493 Size: 711 2018 2702 Fragments arranged by size: 2702 2018 711 BsaBI GATnn'nnATC Cuts at: 2177 2367 5425 2177 Size: 190 3058 2183 Fragments arranged by size: 3058 2183 190 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2082 2690 3238 3620 Size: 53 83 186 936 549 608 548 382 Cuts at: 3620 4255 275 Size: 635 1451 Fragments arranged by size: 1451 936 635 608 549 548 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3864 4262 4536 4683 5094 3864 Size: 398 274 147 411 4201 Fragments arranged by size: 4201 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2241 2271 1178 Size: 30 165 30 838 30 4338 Fragments arranged by size: 4338 838 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2159 2435 2447 2639 1186 Size: 973 276 12 192 3978 Fragments arranged by size: 3978 973 276 192 12 BseYI C'CCAG_C Cuts at: 4634 5130 4634 Size: 496 4935 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2083 2090 2687 1163 Size: 573 347 7 597 3907 Fragments arranged by size: 3907 597 573 347 7 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2547 2881 3642 4670 1540 Size: 275 186 546 334 761 1028 2301 Fragments arranged by size: 2301 1028 761 546 334 275 186 BsiHKAI G_wGCw'C Cuts at: 3492 3577 4648 3492 Size: 85 1071 4275 Fragments arranged by size: 4275 1071 85 BsmI GAATG_Cn' Cuts at: 2942 3035 2942 Size: 93 5338 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2750 3492 3577 4648 1391 Size: 399 960 742 85 1071 2174 Fragments arranged by size: 2174 1071 960 742 399 85 BspEI T'CCGG_A Cuts at: 5094 5094 Size: 5431 BspHI T'CATG_A Cuts at: 2212 3320 5050 2212 Size: 1108 1730 2593 Fragments arranged by size: 2593 1730 1108 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2060 2165 2174 2348 2455 2786 844 Size: 1216 105 9 174 107 331 3489 Fragments arranged by size: 3489 1216 331 174 107 105 9 BsrBI CCG'CTC Cuts at: 1394 1823 2695 3318 1394 Size: 429 872 623 3507 Fragments arranged by size: 3507 872 623 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3926 4100 65 Size: 1183 2678 174 1396 Fragments arranged by size: 2678 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4075 4262 1700 Size: 110 2265 187 2869 Fragments arranged by size: 2869 2265 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5431 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5431 BssSI C'ACGA_G Cuts at: 3491 4503 3491 Size: 1012 4419 BstAPI GCAn_nnn'nTGC Cuts at: 794 2124 794 Size: 1330 4101 BstBI TT'CG_AA Cuts at: 4235 4235 Size: 5431 BstEII G'GTnAC_C Cuts at: 1184 2092 1184 Size: 908 4523 BstXI CCAn_nnnn'nTGG Cuts at: 2375 2375 Size: 5431 BstYI r'GATC_y Cuts at: 1972 2523 2628 2761 3128 3513 3530 4982 5068 Size: 551 105 133 367 385 17 1452 86 Cuts at: 5068 5080 5426 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 551 385 367 346 133 105 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2088 2415 513 Size: 552 1023 327 3529 Fragments arranged by size: 3529 1023 552 327 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2554 487 Size: 683 56 1328 3364 Fragments arranged by size: 3364 1328 683 56 BtsI GCAGTG_nn' Cuts at: 2947 3741 3761 2947 Size: 794 20 4617 Fragments arranged by size: 4617 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5431 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5396 DraI TTT'AAA Cuts at: 2735 2735 Size: 5431 DraIII CAC_nnn'GTG Cuts at: 2673 2673 Size: 5431 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4438 809 Size: 507 3122 1802 Fragments arranged by size: 3122 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2878 3767 5396 Size: 54 1425 50 275 18 1048 889 1629 Cuts at: 5396 8 Size: 43 Fragments arranged by size: 1629 1425 1048 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2878 1537 Size: 275 1066 4090 Fragments arranged by size: 4090 1066 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3361 1353 Size: 2008 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4018 4392 4538 387 Size: 1064 138 2429 374 146 1280 Fragments arranged by size: 2429 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2162 2501 2531 2579 3494 4091 4878 Size: 393 530 339 30 48 915 597 787 Cuts at: 4878 5146 1239 Size: 268 1524 Fragments arranged by size: 1524 915 787 597 530 393 339 268 48 30 EcoNI CCTnn'n_nnAGG Cuts at: 2116 2636 2116 Size: 520 4911 EcoRI G'AATT_C Cuts at: 2740 3118 2740 Size: 378 5053 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2740 2740 Size: 5431 FspI TGC'GCA Cuts at: 3937 3937 Size: 5431 HaeII r_GCGC'y Cuts at: 1678 2693 4578 1678 Size: 1015 1885 2531 Fragments arranged by size: 2531 1885 1015 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2086 2118 4072 4104 4146 Size: 32 135 32 514 32 1954 32 42 Cuts at: 4146 4178 1373 Size: 32 2626 Fragments arranged by size: 2626 1954 514 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2010 2775 3021 669 Size: 988 281 72 765 246 3079 Fragments arranged by size: 3079 988 765 281 246 72 HindIII A'AGCT_T Cuts at: 748 748 Size: 5431 HpaI GTT'AAC Cuts at: 3021 3021 Size: 5431 KasI G'GCGC_C Cuts at: 2689 2689 Size: 5431 KpnI G_GTAC'C Cuts at: 2074 2252 2756 2074 Size: 178 504 4749 Fragments arranged by size: 4749 504 178 MfeI C'AATT_G Cuts at: 3030 3030 Size: 5431 MluI A'CGCG_T Cuts at: 5307 5307 Size: 5431 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4545 4729 1305 Size: 3240 184 2007 Fragments arranged by size: 3240 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3609 Fragments arranged by size: 3609 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2090 2312 2373 3389 3748 3907 518 Size: 648 924 222 61 1016 359 159 2042 Fragments arranged by size: 2042 1016 924 648 359 222 159 61 MspA1I CmG'CkG Cuts at: 1606 2067 2417 2529 2847 3524 4672 4917 1606 Size: 461 350 112 318 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 461 350 318 245 112 NarI GG'CG_CC Cuts at: 2690 2690 Size: 5431 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4879 NdeI CA'TA_TG Cuts at: 387 387 Size: 5431 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5431 NotI GC'GGCC_GC Cuts at: 2878 2878 Size: 5431 NspI r_CATG'y Cuts at: 1132 1229 2789 4334 1132 Size: 97 1560 1545 2229 Fragments arranged by size: 2229 1560 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5431 PciI A'CATG_T Cuts at: 4330 4330 Size: 5431 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5431 PmeI GTTT'AAAC Cuts at: 2735 2735 Size: 5431 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3500 3532 5013 5045 3500 Size: 32 1481 32 3886 Fragments arranged by size: 3886 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5431 PsiI TTA'TAA Cuts at: 3001 3001 Size: 5431 PstI C_TGCA'G Cuts at: 830 2120 2171 2202 2783 830 Size: 1290 51 31 581 3478 Fragments arranged by size: 3478 1290 581 51 31 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5431 SacII CC_GC'GG Cuts at: 2418 2418 Size: 5431 SalI G'TCGA_C Cuts at: 1936 2773 1936 Size: 837 4594 SbfI CC_TGCA'GG Cuts at: 2171 2783 2171 Size: 612 4819 ScaI AGT'ACT Cuts at: 3679 5391 3679 Size: 1712 3719 SfcI C'TryA_G Cuts at: 826 1045 2116 2167 2198 2707 2779 3914 4595 Size: 219 1071 51 31 509 72 1135 681 Cuts at: 4595 4786 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1071 681 509 219 191 72 51 31 SfoI GGC'GCC Cuts at: 2691 2691 Size: 5431 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5431 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5431 SmaI CCC'GGG Cuts at: 1872 2758 1872 Size: 886 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2651 3535 4436 4698 820 Size: 197 926 708 884 901 262 1553 Fragments arranged by size: 1553 926 901 884 708 262 197 SnaBI TAC'GTA Cuts at: 493 3222 493 Size: 2729 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5431 SphI G_CATG'C Cuts at: 1132 2789 1132 Size: 1657 3774 SspI AAT'ATT Cuts at: 5 52 3355 5 Size: 47 3303 2081 Fragments arranged by size: 3303 2081 47 StuI AGG'CCT Cuts at: 1517 2256 1517 Size: 739 4692 StyI C'CwwG_G Cuts at: 513 1065 2870 513 Size: 552 1805 3074 Fragments arranged by size: 3074 1805 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2111 2198 3458 3475 3628 3813 4258 Size: 1255 531 87 1260 17 153 185 445 Cuts at: 4258 325 Size: 1498 Fragments arranged by size: 1498 1260 1255 531 445 185 153 87 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2202 3677 5389 Size: 274 80 33 51 1443 225 1475 1712 Cuts at: 5389 96 Size: 138 Fragments arranged by size: 1712 1475 1443 274 225 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2348 2380 5391 5423 2348 Size: 32 3011 32 2356 Fragments arranged by size: 3011 2356 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5431 XbaI T'CTAG_A Cuts at: 2767 2767 Size: 5431 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5431 XmaI C'CCGG_G Cuts at: 1870 2756 1870 Size: 886 4545 XmnI GAAnn'nnTTC Cuts at: 2564 3560 2564 Size: 996 4435 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3239 276 Size: 53 83 186 936 1705 2468 Fragments arranged by size: 2468 1705 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI XcmI