##################################
# NO CUT ENZYMES (  207 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AfaI        AflII       AflIII      
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaII       AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BseAI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrGI       BsrI        BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstNI       BstPI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       Cfr13I      ClaI        
CpoI        DdeI        DpnII       DraI        DraII       
DraIII      DrdI        DsaI        Eam1105I    Eco47III    
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HgaI        
Hin1I       HincII      HindII      HindIII     HinfI       
HpaI        HphI        KasI        KpnI        KspI        
MaeI        MaeII       MaeIII      MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MunI        MvaI        
NaeI        NarI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      Sau96I      SbfI        ScaI        
ScrFI       SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tsp45I      TspRI       Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AccII          83  CGCG                 CG^CG
AgeI            9  ACCGGT               A^CCGGT
AluI           35  AGCT                 AG^CT
AvaI          104  CTCGGG               C^[CT]CG[GA]G
BanI            3  GGTGCC               G^G[CT][GA]CC
BbvI          130  GCAGC                GCAGC(8/12)
BsaI           18  GGTCTC               GGTCTC(1/5)
BsaWI           9  ACCGGT               [AT]^CCGG[AT]
BsgI           13  GTGCAG               GTGCAG(16/14)
BsiEI          39  CGGCCG               CG[GA][CT]^CG
BsiYI          60  CCTCGGTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           60  CCTCGGTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI          19  GTCTC                GTCTC(1/5)
BsoBI         104  CTCGGG               C^[CT]CG[GA]G
BspMI          15  GCAGGT               ACCTGC(4/8)
BsrBI          25  CCGCTC               CCGCTC(-3/-3)
BsrFI           9  ACCGGT               [GA]^CCGG[CT]
BstF5I        153  GGATG                GGATG(2/0)
BstUI          83  CGCG                 CG^CG
BstXI         173  CCAGAGAATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI           84  GCGC                 GCG^C
Cfr10I          9  ACCGGT               [GA]^CCGG[CT]
EaeI           39  CGGCCG               [CT]^GGCC[GA]
EagI           39  CGGCCG               C^GGCCG
EclXI          39  CGGCCG               C^GGCCG
Eco52I         39  CGGCCG               C^GGCCG
FokI          153  GGATG                GGATG(9/13)
HapII          10  CCGG                 C^CGG
HhaI           84  GCGC                 GCG^C
HinP1I         84  GCGC                 G^CGC
HpaII          10  CCGG                 C^CGG
MseI          202  TTAA                 T^TAA
MspI           10  CCGG                 C^CGG
MvnI           83  CGCG                 CG^CG
PinAI           9  ACCGGT               A^CCGGT
SgrAI           8  CACCGGTG             C[GA]^CCGG[CT]G
Tru9I         202  TTAA                 T^TAA
TseI          130  GCAGC                G^C[AT]GC


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

BsaJI          60  CCTCGG               C^C[ACGT][ACGT]GG
              103  CCTCGG              
BseRI         115  GAGGAG               GAGGAG(10/8)
              120  GAGGAG              
EarI          112  GAAGAG               CTCTTC(1/4)
              136  GAAGAG              
Fnu4HI         41  GCCGC                GC^[ACGT]GC
              130  GCAGC               
HaeIII         40  GGCC                 GG^CC
               69  GGCC                
ItaI           41  GCCGC                GC^[ACGT]GC
              130  GCAGC               
Ksp632I       112  GAAGAG               CTCTTC(1/4)
              136  GAAGAG              
NlaIV           3  GGTGCC               GG[ACGT]^[ACGT]CC
               76  GGAGCC              
Tsp509I        91  AATT                 ^AATT
              179  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

AciI        MboII       MnlI        MwoI