Restriction map for
pFN21AE6170
(Circular) (Six-base) MAPSORT of: pFN21AE6170.seq Check: 7620 from: 1 to: 4912
pFN21AE6170 4912 bp
With 182 enzymes: SgfI *
December 8, 2014 13:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2722 278
Size: 53 83 186 936 1186 2468
Fragments arranged by size:
2468 1186 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2255 1937
Size: 318 4594
Acc65I G'GTAC_C
Cuts at: 2233 2233
Size: 4912
AclI AA'CG_TT
Cuts at: 1563 3039 3412 1563
Size: 1476 373 3063
Fragments arranged by size:
3063 1476 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2975 4359 4627 1632
Size: 1343 1384 268 1917
Fragments arranged by size:
1917 1384 1343 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4715
AflIII A'CryG_T
Cuts at: 1203 3811 4788 1203
Size: 2608 977 1327
Fragments arranged by size:
2608 1327 977
AgeI A'CCGG_T
Cuts at: 2014 3743 2014
Size: 1729 3183
AhdI GACnn_n'nnGTC
Cuts at: 3641 3641
Size: 4912
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4912
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4912
AlwNI CAG_nnn'CTG
Cuts at: 2655 4227 2655
Size: 1572 3340
ApaLI G'TGCA_C
Cuts at: 2969 4125 2969
Size: 1156 3756
ApoI r'AATT_y
Cuts at: 1493 2221 2447 2599 4708 1493
Size: 728 226 152 2109 1697
Fragments arranged by size:
2109 1697 728 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3466 160
Size: 872 2434 1606
Fragments arranged by size:
2434 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4912
AvaI C'yCGr_G
Cuts at: 1870 1943 2109 2237 1870
Size: 73 166 128 4545
Fragments arranged by size:
4545 166 128 73
BamHI G'GATC_C
Cuts at: 2242 2609 2242
Size: 367 4545
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2233 3688 618
Size: 325 217 627 221 225 1455 1842
Fragments arranged by size:
1842 1455 627 325 225 221 217
BanII G_rGCy'C
Cuts at: 1391 2231 1391
Size: 840 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3941
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3103 3137 1903
Size: 34 1166 34 3678
Fragments arranged by size:
3678 1166 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2804 4014 2804
Size: 1210 3702
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4912
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3523 136
Size: 107 122 71 631 605 1851 1525
Fragments arranged by size:
1851 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4907 4907
Size: 4912
BlpI GC'TnA_GC
Cuts at: 2329 2329
Size: 4912
Bme1580I G_kGCm'C
Cuts at: 1790 2973 4129 1790
Size: 1183 1156 2573
Fragments arranged by size:
2573 1183 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4912
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2643 3601 442
Size: 416 53 83 399 24 1226 958 1753
Fragments arranged by size:
1753 1226 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4912
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3572 1239
Size: 2333 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3037 3902 4200 3037
Size: 865 298 3749
Fragments arranged by size:
3749 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2029 3575 882
Size: 617 530 1546 2219
Fragments arranged by size:
2219 1546 617 530
BsaAI yAC'GTr
Cuts at: 493 1204 2703 493
Size: 711 1499 2702
Fragments arranged by size:
2702 1499 711
BsaBI GATnn'nnATC
Cuts at: 4906 4906
Size: 4912
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2719 3101 3736 275
Size: 53 83 186 936 1186 382 635 1451
Fragments arranged by size:
1451 1186 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2014 3345 3743 4017 4164 4575 2014
Size: 1331 398 274 147 411 2351
Fragments arranged by size:
2351 1331 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4687
Fragments arranged by size:
4687 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2140 1186
Size: 949 5 3958
Fragments arranged by size:
3958 949 5
BseYI C'CCAG_C
Cuts at: 4115 4611 4115
Size: 496 4416
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2039 1163
Size: 573 303 4036
Fragments arranged by size:
4036 573 303
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2047 2362 3123 4151 1540
Size: 275 186 46 315 761 1028 2301
Fragments arranged by size:
2301 1028 761 315 275 186 46
BsiHKAI G_wGCw'C
Cuts at: 2973 3058 4129 2973
Size: 85 1071 3756
Fragments arranged by size:
3756 1071 85
BsmI GAATG_Cn'
Cuts at: 2423 2516 2423
Size: 93 4819
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2231 2973 3058 4129 1391
Size: 399 441 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 441 399 85
BspEI T'CCGG_A
Cuts at: 4575 4575
Size: 4912
BspHI T'CATG_A
Cuts at: 2801 4531 2801
Size: 1730 3182
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2011 2267 844
Size: 1167 256 3489
Fragments arranged by size:
3489 1167 256
BsrBI CCG'CTC
Cuts at: 1394 1823 2032 2799 1394
Size: 429 209 767 3507
Fragments arranged by size:
3507 767 429 209
BsrDI GCAATG_nn'
Cuts at: 65 1248 3407 3581 65
Size: 1183 2159 174 1396
Fragments arranged by size:
2159 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2014 3556 3743 1700
Size: 110 204 1542 187 2869
Fragments arranged by size:
2869 1542 204 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4912
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4912
BssSI C'ACGA_G
Cuts at: 2972 3984 2972
Size: 1012 3900
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4912
BstBI TT'CG_AA
Cuts at: 3716 3716
Size: 4912
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4912
BstXI CCAn_nnnn'nTGG
Cuts at: 2185 2185
Size: 4912
BstYI r'GATC_y
Cuts at: 1972 2242 2609 2994 3011 4463 4549 4561 4907
Size: 270 367 385 17 1452 86 12 346
Cuts at: 4907 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 270 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3421
Fragments arranged by size:
3421 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4173
Fragments arranged by size:
4173 683 56
BtsI GCAGTG_nn'
Cuts at: 2428 3222 3242 2428
Size: 794 20 4098
Fragments arranged by size:
4098 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4912
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4877
DraI TTT'AAA
Cuts at: 2216 2216
Size: 4912
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3919 809
Size: 507 2603 1802
Fragments arranged by size:
2603 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2044 2359 3248
Size: 54 1425 50 275 18 214 315 889
Cuts at: 3248 4877 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 315 275 214 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2044 2359 1537
Size: 275 232 315 4090
Fragments arranged by size:
4090 315 275 232
EarI CTCTTCn'nnn_
Cuts at: 1353 2112 2136 2842 1353
Size: 759 24 706 3423
Fragments arranged by size:
3423 759 706 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2044 3499 3873 4019 387
Size: 1064 138 455 1455 374 146 1280
Fragments arranged by size:
1455 1280 1064 455 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2975 3572 4359 4627 1239
Size: 393 1343 597 787 268 1524
Fragments arranged by size:
1524 1343 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2221 2599 2221
Size: 378 4534
FspI TGC'GCA
Cuts at: 3418 3418
Size: 4912
HaeII r_GCGC'y
Cuts at: 1678 4059 1678
Size: 2381 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3553 3585 3627 3659 1373
Size: 32 135 32 1981 32 42 32 2626
Fragments arranged by size:
2626 1981 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2256 2502 669
Size: 988 281 318 246 3079
Fragments arranged by size:
3079 988 318 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4912
HpaI GTT'AAC
Cuts at: 2502 2502
Size: 4912
KpnI G_GTAC'C
Cuts at: 2237 2237
Size: 4912
MfeI C'AATT_G
Cuts at: 2511 2511
Size: 4912
MluI A'CGCG_T
Cuts at: 4788 4788
Size: 4912
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4026 4210 1305
Size: 2721 184 2007
Fragments arranged by size:
2721 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3090
Fragments arranged by size:
3090 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2870 3229 3388 518
Size: 648 1704 359 159 2042
Fragments arranged by size:
2042 1704 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2328 3005 4153 4398 1606
Size: 722 677 1148 245 2120
Fragments arranged by size:
2120 1148 722 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3421
Fragments arranged by size:
3421 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4912
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4912
NotI GC'GGCC_GC
Cuts at: 2359 2359
Size: 4912
NspI r_CATG'y
Cuts at: 1132 1229 2270 3815 1132
Size: 97 1041 1545 2229
Fragments arranged by size:
2229 1545 1041 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4912
PciI A'CATG_T
Cuts at: 3811 3811
Size: 4912
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4912
PmeI GTTT'AAAC
Cuts at: 2216 2216
Size: 4912
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2981 3013 4494 4526 2981
Size: 32 1481 32 3367
Fragments arranged by size:
3367 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4912
PsiI TTA'TAA
Cuts at: 2482 2482
Size: 4912
PstI C_TGCA'G
Cuts at: 830 2264 830
Size: 1434 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4912
SalI G'TCGA_C
Cuts at: 1936 2254 1936
Size: 318 4594
SbfI CC_TGCA'GG
Cuts at: 2264 2264
Size: 4912
ScaI AGT'ACT
Cuts at: 3160 4872 3160
Size: 1712 3200
SfcI C'TryA_G
Cuts at: 826 1045 2260 3395 4076 4267 826
Size: 219 1215 1135 681 191 1471
Fragments arranged by size:
1471 1215 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4912
SgrAI Cr'CCGG_yG
Cuts at: 1700 2014 1700
Size: 314 4598
SmaI CCC'GGG
Cuts at: 1872 2239 1872
Size: 367 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3016 3917 4179 820
Size: 197 926 1073 901 262 1553
Fragments arranged by size:
1553 1073 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2703 493
Size: 2210 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4912
SphI G_CATG'C
Cuts at: 1132 2270 1132
Size: 1138 3774
SspI AAT'ATT
Cuts at: 5 52 2836 5
Size: 47 2784 2081
Fragments arranged by size:
2784 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4912
StyI C'CwwG_G
Cuts at: 513 1065 2004 2351 513
Size: 552 939 347 3074
Fragments arranged by size:
3074 939 552 347
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2939 2956 3109 3294 3739 325
Size: 1255 1359 17 153 185 445 1498
Fragments arranged by size:
1498 1359 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3158 4870 96
Size: 274 80 33 51 1443 1181 1712 138
Fragments arranged by size:
1712 1443 1181 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4872 4904 4872
Size: 32 4880
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4912
XbaI T'CTAG_A
Cuts at: 2248 2248
Size: 4912
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4912
XmaI C'CCGG_G
Cuts at: 1870 2237 1870
Size: 367 4545
XmnI GAAnn'nnTTC
Cuts at: 3041 3041
Size: 4912
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2720 276
Size: 53 83 186 936 1186 2468
Fragments arranged by size:
2468 1186 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI