Restriction map for
pFN21AE3248
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII AccIII AclI AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspEI AspI AvaI AvaII AviII
AvrII BbeI BbrPI BbsI BbvCI
BbvI BcgI BglII BlnI BpuAI
BsaAI BsaI BsaWI BseAI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DraII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoT22I FseI FspI HaeII
HpaI KasI KspI MfeI MluI
MroI MspA1I MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TseI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 123 GTCGAC GT^[AC][GT]AC
AccII 1429 CGCG CG^CG
AcyI 16 GACGCC G[GA]^CG[CT]C
AflII 981 CTTAAG C^TTAAG
Asp700I 200 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1646 GTGCTC G[AT]GC[AT]^C
BalI 279 TGGCCA TGG^CCA
BanII 73 GAGCCC G[GA]GC[CT]^C
BciVI 1636 GTATCC GTATCC(6/5)
BclI 1139 TGATCA T^GATCA
BcnI 168 CCGGG CC^[GC]GG
BfrI 981 CTTAAG C^TTAAG
BglI 1389 GCCAAACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 543 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 543 GCTAAGC GC^T[ACGT]AGC
BsaHI 16 GACGCC G[GA]^CG[CT]C
BseRI 454 GAGGAG GAGGAG(10/8)
BsgI 866 GTGCAG GTGCAG(16/14)
BsiEI 125 CGACCG CG[GA][CT]^CG
BsiHKAI 1646 GTGCTC G[AT]GC[AT]^C
Bsp1407I 729 TGTACA T^GTACA
BsrFI 1394 ACCGGC [GA]^CCGG[CT]
BsrGI 729 TGTACA T^GTACA
BstAPI 1380 GCAAATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1429 CGCG CG^CG
CelII 543 GCTAAGC GC^T[ACGT]AGC
Cfr10I 1394 ACCGGC [GA]^CCGG[CT]
Cfr13I 395 GGGCC G^G[ACGT]CC
DraI 1566 TTTAAA TTT^AAA
DraIII 99 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 895 CCATGG C^C[GA][CT]GG
EaeI 279 TGGCCA [CT]^GGCC[GA]
EcoRI 597 GAATTC G^AATTC
EcoRV 840 GATATC GAT^ATC
FauI 47 GCGGG CCCGC(4/6)
FbaI 1139 TGATCA T^GATCA
Hin1I 16 GACGCC G[GA]^CG[CT]C
HindIII 265 AAGCTT A^AGCTT
MluNI 279 TGGCCA TGG^CCA
MscI 279 TGGCCA TGG^CCA
MslI 896 CATGGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 1429 CGCG CG^CG
NciI 168 CCGGG CC^[GC]GG
NcoI 895 CCATGG C^CATGG
PstI 1028 CTGCAG CTGCA^G
SalI 123 GTCGAC G^TCGAC
SapI 494 GAAGAGC GCTCTTC(1/4)
Sau96I 395 GGGCC G^G[ACGT]CC
SspBI 729 TGTACA T^GTACA
XmnI 200 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 188 GGTACC G^GTACC
923 GGTACC
Asp718I 188 GGTACC G^GTACC
923 GGTACC
BamHI 1197 GGATCC G^GATCC
1405 GGATCC
BfaI 1619 CTAG C^TAG
1664 CTAG
BmyI 73 GAGCCC G[AGT]GC[ACT]^C
1646 GTGCTC
BpmI 1445 CTCCAG CTGGAG(16/14)
665 CTGGAG
BsaBI 304 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1414 GATGAGTATC
BsmAI 1111 GAGAC GTCTC(1/5)
1314 GAGAC
Bsp1286I 73 GAGCCC G[AGT]GC[ACT]^C
1646 GTGCTC
BssSI 988 CACGAG CACGAG(-5/-1)
67 CTCGTG
BstNI 254 CCAGG CC^[AT]GG
337 CCTGG
Cac8I 263 GCAAGC GC[ACGT]^[ACGT]GC
397 GCCAGC
CfoI 1064 GCGC GCG^C
1430 GCGC
EcoRII 254 CCAGG ^CC[AT]GG
337 CCTGG
EcoT14I 846 CCTTGG C^C[AT][AT]GG
895 CCATGG
Fnu4HI 164 GCCGC GC^[ACGT]GC
382 GCGGC
HaeIII 280 GGCC GG^CC
396 GGCC
HapII 168 CCGG C^CGG
1395 CCGG
HgaI 16 GACGC GACGC(5/10)
274 GCGTC
HhaI 1064 GCGC GCG^C
1430 GCGC
HinP1I 1064 GCGC G^CGC
1430 GCGC
HincII 123 GTCGAC GT[CT]^[GA]AC
214 GTCAAC
HindII 123 GTCGAC GT[CT]^[GA]AC
214 GTCAAC
HpaII 168 CCGG C^CGG
1395 CCGG
ItaI 164 GCCGC GC^[ACGT]GC
382 GCGGC
KpnI 188 GGTACC GGTAC^C
923 GGTACC
MaeI 1619 CTAG C^TAG
1664 CTAG
MaeII 218 ACGT A^CGT
938 ACGT
MamI 304 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1414 GATGAGTATC
MspI 168 CCGG C^CGG
1395 CCGG
MvaI 254 CCAGG CC^[AT]GG
337 CCTGG
SfcI 1028 CTGCAG C^T[GA][CT]AG
1657 CTACAG
SmlI 981 CTTAAG C^T[CT][GA]AG
1539 CTCAAG
StyI 846 CCTTGG C^C[AT][AT]GG
895 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BanI BsaJI BsiYI BslI
BsmFI BsrI BssKI BstF5I BstYI
DdeI DpnII EarI FokI HinfI
HphI Ksp632I MaeIII MboI MboII
MflI MnlI MseI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
ScrFI SfaNI TaqI TfiI Tru9I
Tsp45I Tsp509I TspRI TthHB8I XhoII