(Circular) (Six-base) MAPSORT of: pFN21AE3248.seq Check: 3781 from: 1 to: 6418 pFN21AE3248 6418 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4228 278 Size: 53 83 186 936 2692 2468 Fragments arranged by size: 2692 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2129 3761 1937 Size: 192 1632 4594 Fragments arranged by size: 4594 1632 192 Acc65I G'GTAC_C Cuts at: 2193 2928 3739 2193 Size: 735 811 4872 Fragments arranged by size: 4872 811 735 AclI AA'CG_TT Cuts at: 1563 4545 4918 1563 Size: 2982 373 3063 Fragments arranged by size: 3063 2982 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2748 3347 4481 5865 6133 1632 Size: 1116 599 1134 1384 268 1917 Fragments arranged by size: 1917 1384 1134 1116 599 268 AflII C'TTAA_G Cuts at: 820 1017 2986 820 Size: 197 1969 4252 Fragments arranged by size: 4252 1969 197 AflIII A'CryG_T Cuts at: 1203 5317 6294 1203 Size: 4114 977 1327 Fragments arranged by size: 4114 1327 977 AgeI A'CCGG_T Cuts at: 5249 5249 Size: 6418 AhdI GACnn_n'nnGTC Cuts at: 5147 5147 Size: 6418 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6418 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6418 AlwNI CAG_nnn'CTG Cuts at: 4161 5733 4161 Size: 1572 4846 ApaLI G'TGCA_C Cuts at: 4475 5631 4475 Size: 1156 5262 ApoI r'AATT_y Cuts at: 1493 2209 2602 3616 3727 3953 4105 6214 1493 Size: 716 393 1014 111 226 152 2109 1697 Fragments arranged by size: 2109 1697 1014 716 393 226 152 111 AseI AT'TA_AT Cuts at: 160 1032 4972 160 Size: 872 3940 1606 Fragments arranged by size: 3940 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6418 AvaI C'yCGr_G Cuts at: 1870 1943 3743 1870 Size: 73 1800 4545 Fragments arranged by size: 4545 1800 73 BamHI G'GATC_C Cuts at: 3202 3410 3748 4115 3202 Size: 208 338 367 5505 Fragments arranged by size: 5505 367 338 208 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2193 2918 2928 3391 3739 Size: 325 217 627 406 725 10 463 348 Cuts at: 3739 5194 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 725 627 463 406 348 325 217 10 BanII G_rGCy'C Cuts at: 1391 2082 3737 1391 Size: 691 1655 4072 Fragments arranged by size: 4072 1655 691 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5447 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4609 4643 1903 Size: 34 2672 34 3678 Fragments arranged by size: 3678 2672 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3652 4310 5520 3652 Size: 658 1210 4550 Fragments arranged by size: 4550 1210 658 BclI T'GATC_A Cuts at: 1548 3144 1548 Size: 1596 4822 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3400 5029 136 Size: 107 122 71 631 605 1728 1629 1525 Fragments arranged by size: 1728 1629 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6413 6413 Size: 6418 BlpI GC'TnA_GC Cuts at: 2549 3835 2549 Size: 1286 5132 Bme1580I G_kGCm'C Cuts at: 1790 4479 5635 1790 Size: 2689 1156 2573 Fragments arranged by size: 2689 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6418 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4149 5107 442 Size: 416 53 83 399 24 2732 958 1753 Fragments arranged by size: 2732 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6418 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3559 3559 Size: 6418 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2691 3435 5078 1239 Size: 1452 744 1643 2579 Fragments arranged by size: 2579 1643 1452 744 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3529 4543 5408 5706 3529 Size: 1014 865 298 4241 Fragments arranged by size: 4241 1014 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5081 882 Size: 617 3582 2219 Fragments arranged by size: 3582 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4209 493 Size: 711 3005 2702 Fragments arranged by size: 3005 2702 711 BsaBI GATnn'nnATC Cuts at: 2313 3423 6412 2313 Size: 1110 2989 2319 Fragments arranged by size: 2989 2319 1110 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2022 4225 4607 5242 Size: 53 83 186 936 489 2203 382 635 Cuts at: 5242 275 Size: 1451 Fragments arranged by size: 2203 1451 936 635 489 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4851 5249 5523 5670 6081 4851 Size: 398 274 147 411 5188 Fragments arranged by size: 5188 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2821 2851 1178 Size: 30 165 30 1418 30 4745 Fragments arranged by size: 4745 1418 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2474 1186 Size: 1288 5130 BseYI C'CCAG_C Cuts at: 5621 6117 5621 Size: 496 5922 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2892 1163 Size: 573 1156 4689 Fragments arranged by size: 4689 1156 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2133 3868 4629 5657 1540 Size: 275 186 132 1735 761 1028 2301 Fragments arranged by size: 2301 1735 1028 761 275 186 132 BsiHKAI G_wGCw'C Cuts at: 3655 4479 4564 5635 3655 Size: 824 85 1071 4438 Fragments arranged by size: 4438 1071 824 85 BsmI GAATG_Cn' Cuts at: 3929 4022 3929 Size: 93 6325 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 3655 3737 4479 4564 5635 1391 Size: 399 292 1573 82 742 85 1071 2174 Fragments arranged by size: 2174 1573 1071 742 399 292 85 82 BspEI T'CCGG_A Cuts at: 6081 6081 Size: 6418 BspHI T'CATG_A Cuts at: 4307 6037 4307 Size: 1730 4688 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3773 844 Size: 2929 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4305 1394 Size: 429 2482 3507 Fragments arranged by size: 3507 2482 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4913 5087 65 Size: 1183 3665 174 1396 Fragments arranged by size: 3665 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3399 5062 5249 1700 Size: 110 1589 1663 187 2869 Fragments arranged by size: 2869 1663 1589 187 110 BsrGI T'GTAC_A Cuts at: 96 2734 96 Size: 2638 3780 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6418 BssSI C'ACGA_G Cuts at: 2072 2993 4478 5490 2072 Size: 921 1485 1012 3000 Fragments arranged by size: 3000 1485 1012 921 BstAPI GCAn_nnn'nTGC Cuts at: 794 3391 794 Size: 2597 3821 BstBI TT'CG_AA Cuts at: 5222 5222 Size: 6418 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6418 BstYI r'GATC_y Cuts at: 1972 2055 3202 3410 3748 4115 4500 4517 5969 Size: 83 1147 208 338 367 385 17 1452 Cuts at: 5969 6055 6067 6413 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1147 385 367 346 338 208 86 83 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2900 513 Size: 552 939 896 4031 Fragments arranged by size: 4031 939 896 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2519 487 Size: 683 56 1293 4386 Fragments arranged by size: 4386 1293 683 56 BtsI GCAGTG_nn' Cuts at: 3934 4728 4748 3934 Size: 794 20 5604 Fragments arranged by size: 5604 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6418 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6383 DraI TTT'AAA Cuts at: 3573 3722 3573 Size: 149 6269 DraIII CAC_nnn'GTG Cuts at: 2109 2109 Size: 6418 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5425 809 Size: 507 4109 1802 Fragments arranged by size: 4109 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2284 3865 4754 Size: 54 1425 50 275 18 454 1581 889 Cuts at: 4754 6383 8 Size: 1629 43 Fragments arranged by size: 1629 1581 1425 889 454 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3865 1537 Size: 275 2053 4090 Fragments arranged by size: 4090 2053 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2350 2361 2463 2494 3123 4348 1353 Size: 997 11 102 31 629 1225 3423 Fragments arranged by size: 3423 1225 997 629 102 31 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5005 5379 5525 387 Size: 1064 138 3416 374 146 1280 Fragments arranged by size: 3416 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2691 2748 3347 3435 4481 5078 5865 Size: 393 1059 57 599 88 1046 597 787 Cuts at: 5865 6133 1239 Size: 268 1524 Fragments arranged by size: 1524 1059 1046 787 599 597 393 268 88 57 EcoRI G'AATT_C Cuts at: 2602 3727 4105 2602 Size: 1125 378 4915 Fragments arranged by size: 4915 1125 378 EcoRV GAT'ATC Cuts at: 2847 2847 Size: 6418 FspI TGC'GCA Cuts at: 4924 4924 Size: 6418 HaeII r_GCGC'y Cuts at: 1678 5565 1678 Size: 3887 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2744 2776 2821 2853 3466 Size: 32 135 32 1172 32 45 32 613 Cuts at: 3466 3498 5059 5091 5133 5165 1373 Size: 32 1561 32 42 32 2626 Fragments arranged by size: 2626 1561 1172 613 135 45 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2130 2221 3762 4008 669 Size: 988 281 192 91 1541 246 3079 Fragments arranged by size: 3079 1541 988 281 246 192 91 HindIII A'AGCT_T Cuts at: 748 2270 748 Size: 1522 4896 HpaI GTT'AAC Cuts at: 4008 4008 Size: 6418 KpnI G_GTAC'C Cuts at: 2197 2932 3743 2197 Size: 735 811 4872 Fragments arranged by size: 4872 811 735 MfeI C'AATT_G Cuts at: 4017 4017 Size: 6418 MluI A'CGCG_T Cuts at: 6294 6294 Size: 6418 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2839 3551 5532 5716 1305 Size: 1534 712 1981 184 2007 Fragments arranged by size: 2007 1981 1534 712 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2286 10 Size: 54 1425 343 454 4142 Fragments arranged by size: 4142 1425 454 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2905 4376 4735 4894 518 Size: 648 1739 1471 359 159 2042 Fragments arranged by size: 2042 1739 1471 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3834 4511 5659 5904 1606 Size: 2228 677 1148 245 2120 Fragments arranged by size: 2228 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2900 513 Size: 552 939 896 4031 Fragments arranged by size: 4031 939 896 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6418 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6418 NotI GC'GGCC_GC Cuts at: 3865 3865 Size: 6418 NspI r_CATG'y Cuts at: 1132 1229 3776 5321 1132 Size: 97 2547 1545 2229 Fragments arranged by size: 2547 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2259 1348 Size: 911 5507 PciI A'CATG_T Cuts at: 5317 5317 Size: 6418 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6418 PmeI GTTT'AAAC Cuts at: 3722 3722 Size: 6418 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4487 4519 6000 6032 4487 Size: 32 1481 32 4873 Fragments arranged by size: 4873 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6418 PsiI TTA'TAA Cuts at: 3988 3988 Size: 6418 PstI C_TGCA'G Cuts at: 830 3037 3770 830 Size: 2207 733 3478 Fragments arranged by size: 3478 2207 733 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6418 SalI G'TCGA_C Cuts at: 1936 2128 3760 1936 Size: 192 1632 4594 Fragments arranged by size: 4594 1632 192 SapI GCTCTTCn'nnn_ Cuts at: 2494 2494 Size: 6418 SbfI CC_TGCA'GG Cuts at: 3770 3770 Size: 6418 ScaI AGT'ACT Cuts at: 4666 6378 4666 Size: 1712 4706 SfcI C'TryA_G Cuts at: 826 1045 3033 3662 3766 4901 5582 5773 826 Size: 219 1988 629 104 1135 681 191 1471 Fragments arranged by size: 1988 1471 1135 681 629 219 191 104 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6418 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6418 SmaI CCC'GGG Cuts at: 1872 3745 1872 Size: 1873 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2986 3544 4522 5423 5685 820 Size: 197 926 1043 558 978 901 262 1553 Fragments arranged by size: 1553 1043 978 926 901 558 262 197 SnaBI TAC'GTA Cuts at: 493 4209 493 Size: 3716 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6418 SphI G_CATG'C Cuts at: 1132 3776 1132 Size: 2644 3774 SspI AAT'ATT Cuts at: 5 52 4342 5 Size: 47 4290 2081 Fragments arranged by size: 4290 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6418 StyI C'CwwG_G Cuts at: 513 1065 2004 2851 2900 3857 513 Size: 552 939 847 49 957 3074 Fragments arranged by size: 3074 957 939 847 552 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2644 3054 4445 4462 4615 4800 5245 Size: 1255 1064 410 1391 17 153 185 445 Cuts at: 5245 325 Size: 1498 Fragments arranged by size: 1498 1391 1255 1064 445 410 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2734 4664 6376 Size: 274 80 33 51 1443 757 1930 1712 Cuts at: 6376 96 Size: 138 Fragments arranged by size: 1930 1712 1443 757 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3508 3540 6378 6410 3508 Size: 32 2838 32 3516 Fragments arranged by size: 3516 2838 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6418 XbaI T'CTAG_A Cuts at: 3754 3754 Size: 6418 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6418 XmaI C'CCGG_G Cuts at: 1870 3743 1870 Size: 1873 4545 XmnI GAAnn'nnTTC Cuts at: 2209 4547 2209 Size: 2338 4080 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4226 276 Size: 53 83 186 936 2692 2468 Fragments arranged by size: 2692 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI