Restriction map for
pFN21AE2320
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI AspEI AspI
AviII AvrII BalI BamHI BbrPI
BcgI BclI BfrI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KspI MamI MluI
MluNI MroI MscI MspA1I NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 579 GGTACC G^GTACC
AccI 406 GTCTAC GT^[AC][GT]AC
AcyI 150 GGCGCC G[GA]^CG[CT]C
AflIII 251 ACGTGT A^C[GA][CT]GT
AlwNI 1420 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 376 CCTCAGG CC^T[ACGT]AGG
Asp700I 1692 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 579 GGTACC G^GTACC
BbeI 150 GGCGCC GGCGC^C
BbsI 563 GAAGAC GAAGAC(2/6)
BfaI 500 CTAG C^TAG
BglI 277 GCCAAGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 563 GAAGAC GAAGAC(2/6)
BsaHI 150 GGCGCC G[GA]^CG[CT]C
BsaI 1612 GAGACC GGTCTC(1/5)
BsgI 1326 CTGCAC GTGCAG(16/14)
Bsp1407I 626 TGTACA T^GTACA
BsrFI 1529 ACCGGC [GA]^CCGG[CT]
BsrGI 626 TGTACA T^GTACA
Bsu36I 376 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1529 ACCGGC [GA]^CCGG[CT]
DsaI 1457 CCATGG C^C[GA][CT]GG
EaeI 284 CGGCCA [CT]^GGCC[GA]
EarI 764 GAAGAG CTCTTC(1/4)
Eco81I 376 CCTCAGG CC^T[ACGT]AGG
EcoT14I 1457 CCATGG C^C[AT][AT]GG
Hin1I 150 GGCGCC G[GA]^CG[CT]C
KasI 150 GGCGCC G^GCGCC
KpnI 579 GGTACC GGTAC^C
Ksp632I 764 GAAGAG CTCTTC(1/4)
MaeI 500 CTAG C^TAG
MfeI 1010 CAATTG C^AATTG
MseI 682 TTAA T^TAA
MunI 1010 CAATTG C^AATTG
NarI 150 GGCGCC GG^CGCC
NcoI 1457 CCATGG C^CATGG
NdeI 1091 CATATG CA^TATG
PflMI 1249 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfcI 1589 CTACAG C^T[GA][CT]AG
SfoI 150 GGCGCC GGC^GCC
SmaI 1616 CCCGGG CCC^GGG
SspBI 626 TGTACA T^GTACA
StyI 1457 CCATGG C^C[AT][AT]GG
Tru9I 682 TTAA T^TAA
Van91I 1249 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1457 CCATGGATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1616 CCCGGG C^CCGGG
XmaI 1616 CCCGGG C^CCGGG
XmnI 1692 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccII 132 CGCG CG^CG
394 CGCG
AspHI 270 GAGCAC G[AT]GC[AT]^C
421 GAGCAC
AvaI 1616 CCCGGG C^[CT]CG[GA]G
1679 CCCGAG
BanII 61 GGGCTC G[GA]GC[CT]^C
1602 GGGCTC
BbvCI 1000 CCTCAGC CCTCAGC(-5/-2)
1168 CCTCAGC
BciVI 869 GTATCC GTATCC(6/5)
1344 GTATCC
BlpI 418 GCTGAGC GC^T[ACGT]AGC
462 GCTGAGC
BpmI 1605 CTCCAG CTGGAG(16/14)
1256 CTGGAG
Bpu1102I 418 GCTGAGC GC^T[ACGT]AGC
462 GCTGAGC
BsiHKAI 270 GAGCAC G[AT]GC[AT]^C
421 GAGCAC
BsoBI 1616 CCCGGG C^[CT]CG[GA]G
1679 CCCGAG
BstUI 132 CGCG CG^CG
394 CGCG
CelII 418 GCTGAGC GC^T[ACGT]AGC
462 GCTGAGC
FauI 205 CCCGC CCCGC(4/6)
237 CCCGC
HaeII 150 GGCGCC [GA]GCGC^[CT]
842 AGCGCC
HgaI 728 GACGC GACGC(5/10)
143 GCGTC
MvnI 132 CGCG CG^CG
394 CGCG
Tsp45I 145 GTCAC ^GT[GC]AC
427 GTCAC
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanI BbvI BcnI
BmyI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EcoRI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI TseI Tsp509I
TspRI TthHB8I XhoII