(Circular) (Six-base) MAPSORT of: pFN21AE2320.seq Check: 2505 from: 1 to: 6463 pFN21AE2320 6463 bp With 182 enzymes: SgfI * December 28, 2011 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4273 278 Size: 53 83 186 936 2737 2468 Fragments arranged by size: 2737 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2412 3806 1937 Size: 475 1394 4594 Fragments arranged by size: 4594 1394 475 Acc65I G'GTAC_C Cuts at: 2584 3784 2584 Size: 1200 5263 AclI AA'CG_TT Cuts at: 1563 4590 4963 1563 Size: 3027 373 3063 Fragments arranged by size: 3063 3027 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2336 3047 4526 5910 6178 1632 Size: 704 711 1479 1384 268 1917 Fragments arranged by size: 1917 1479 1384 711 704 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6266 AflIII A'CryG_T Cuts at: 1203 2256 5362 6339 1203 Size: 1053 3106 977 1327 Fragments arranged by size: 3106 1327 1053 977 AgeI A'CCGG_T Cuts at: 5294 5294 Size: 6463 AhdI GACnn_n'nnGTC Cuts at: 5192 5192 Size: 6463 AleI CACnn'nnGTG Cuts at: 1166 2438 1166 Size: 1272 5191 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2362 3266 1184 Size: 1178 904 4381 Fragments arranged by size: 4381 1178 904 AlwNI CAG_nnn'CTG Cuts at: 3430 4206 5778 3430 Size: 776 1572 4115 Fragments arranged by size: 4115 1572 776 ApaLI G'TGCA_C Cuts at: 4520 5676 4520 Size: 1156 5307 ApoI r'AATT_y Cuts at: 1493 2477 2615 3548 3772 3998 4150 6259 1493 Size: 984 138 933 224 226 152 2109 1697 Fragments arranged by size: 2109 1697 984 933 226 224 152 138 AseI AT'TA_AT Cuts at: 160 1032 5017 160 Size: 872 3985 1606 Fragments arranged by size: 3985 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6463 AvaI C'yCGr_G Cuts at: 1870 1943 3621 3684 3788 1870 Size: 73 1678 63 104 4545 Fragments arranged by size: 4545 1678 104 73 63 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3332 3365 3332 Size: 33 6430 BamHI G'GATC_C Cuts at: 3793 4160 3793 Size: 367 6096 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2155 2451 2584 3242 3784 Size: 325 217 627 368 296 133 658 542 Cuts at: 3784 5239 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 658 627 542 368 325 296 217 133 BanII G_rGCy'C Cuts at: 1391 2070 3611 3782 1391 Size: 679 1541 171 4072 Fragments arranged by size: 4072 1541 679 171 BbeI G_GCGC'C Cuts at: 2159 2159 Size: 6463 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2575 928 Size: 971 676 4816 Fragments arranged by size: 4816 971 676 BbvCI CC'TCA_GC Cuts at: 3006 3174 3006 Size: 168 6295 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4654 4688 1903 Size: 34 2717 34 3678 Fragments arranged by size: 3678 2717 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2885 3360 4355 5565 2885 Size: 475 995 1210 3783 Fragments arranged by size: 3783 1210 995 475 BclI T'GATC_A Cuts at: 1548 1548 Size: 6463 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2288 5074 136 Size: 107 122 71 631 605 616 2786 1525 Fragments arranged by size: 2786 1525 631 616 605 122 107 71 BglII A'GATC_T Cuts at: 6458 6458 Size: 6463 BlpI GC'TnA_GC Cuts at: 2424 2468 3880 2424 Size: 44 1412 5007 Fragments arranged by size: 5007 1412 44 Bme1580I G_kGCm'C Cuts at: 1790 2456 3245 4524 5680 1790 Size: 666 789 1279 1156 2573 Fragments arranged by size: 2573 1279 1156 789 666 BmgBI CAC'GTC Cuts at: 1318 2257 2431 1318 Size: 939 174 5350 Fragments arranged by size: 5350 939 174 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2536 4194 5152 Size: 416 53 83 399 24 1119 1658 958 Cuts at: 5152 442 Size: 1753 Fragments arranged by size: 1753 1658 1119 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6463 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3475 3475 Size: 6463 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3282 3595 5123 1239 Size: 2043 313 1528 2579 Fragments arranged by size: 2579 2043 1528 313 Bpu10I CC'TnA_GC Cuts at: 3006 3174 3492 3006 Size: 168 318 5977 Fragments arranged by size: 5977 318 168 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4588 5453 5751 4588 Size: 865 298 5300 Fragments arranged by size: 5300 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3611 5126 882 Size: 617 2112 1515 2219 Fragments arranged by size: 2219 2112 1515 617 BsaAI yAC'GTr Cuts at: 493 1204 4254 493 Size: 711 3050 2702 Fragments arranged by size: 3050 2702 711 BsaBI GATnn'nnATC Cuts at: 6457 6457 Size: 6463 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2156 4270 4652 5287 Size: 53 83 186 936 623 2114 382 635 Cuts at: 5287 275 Size: 1451 Fragments arranged by size: 2114 1451 936 635 623 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4896 5294 5568 5715 6126 4896 Size: 398 274 147 411 5233 Fragments arranged by size: 5233 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2362 2392 3536 3566 1178 Size: 30 165 30 959 30 1144 30 4075 Fragments arranged by size: 4075 1144 959 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2095 2370 3544 3767 1186 Size: 909 275 1174 223 3882 Fragments arranged by size: 3882 1174 909 275 223 BseYI C'CCAG_C Cuts at: 2177 5666 6162 2177 Size: 3489 496 2478 Fragments arranged by size: 3489 2478 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3316 1163 Size: 573 1580 4310 Fragments arranged by size: 4310 1580 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3913 4674 5702 1540 Size: 275 186 1912 761 1028 2301 Fragments arranged by size: 2301 1912 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2279 2430 4524 4609 5680 2279 Size: 151 2094 85 1071 3062 Fragments arranged by size: 3062 2094 1071 151 85 BsmI GAATG_Cn' Cuts at: 3974 4067 3974 Size: 93 6370 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2279 2430 2456 3245 3611 3782 Size: 399 280 209 151 26 789 366 171 Cuts at: 3782 4524 4609 5680 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 789 742 399 366 280 209 171 151 85 26 BspEI T'CCGG_A Cuts at: 6126 6126 Size: 6463 BspHI T'CATG_A Cuts at: 4352 6082 4352 Size: 1730 4733 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3818 844 Size: 2974 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4350 1394 Size: 429 2527 3507 Fragments arranged by size: 3507 2527 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4958 5132 65 Size: 1183 3710 174 1396 Fragments arranged by size: 3710 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3534 5107 5294 1700 Size: 110 1724 1573 187 2869 Fragments arranged by size: 2869 1724 1573 187 110 BsrGI T'GTAC_A Cuts at: 96 2631 96 Size: 2535 3928 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6463 BssSI C'ACGA_G Cuts at: 4523 5535 4523 Size: 1012 5451 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6463 BstBI TT'CG_AA Cuts at: 5267 5267 Size: 6463 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6463 BstYI r'GATC_y Cuts at: 1972 2554 2751 2903 3793 4160 4545 4562 6014 Size: 582 197 152 890 367 385 17 1452 Cuts at: 6014 6100 6112 6458 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 890 582 385 367 346 197 152 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2382 2382 Size: 6463 BtgI C'CryG_G Cuts at: 513 1065 3462 513 Size: 552 2397 3514 Fragments arranged by size: 3514 2397 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5724 Fragments arranged by size: 5724 683 56 BtsI GCAGTG_nn' Cuts at: 3328 3592 3979 4773 4793 3328 Size: 264 387 794 20 4998 Fragments arranged by size: 4998 794 387 264 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6463 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6428 DraI TTT'AAA Cuts at: 3767 3767 Size: 6463 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5470 809 Size: 507 4154 1802 Fragments arranged by size: 4154 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2289 3910 4799 Size: 54 1425 50 275 18 459 1621 889 Cuts at: 4799 6428 8 Size: 1629 43 Fragments arranged by size: 1629 1621 1425 889 459 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3910 1537 Size: 275 2098 4090 Fragments arranged by size: 4090 2098 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2764 4393 1353 Size: 1411 1629 3423 Fragments arranged by size: 3423 1629 1411 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2798 3650 5050 5424 5570 387 Size: 1064 138 1209 852 1400 374 146 1280 Fragments arranged by size: 1400 1280 1209 1064 852 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2336 3047 3282 3595 4526 5123 5910 Size: 393 704 711 235 313 931 597 787 Cuts at: 5910 6178 1239 Size: 268 1524 Fragments arranged by size: 1524 931 787 711 704 597 393 313 268 235 EcoRI G'AATT_C Cuts at: 2477 2615 3548 3772 4150 2477 Size: 138 933 224 378 4790 Fragments arranged by size: 4790 933 378 224 138 FspI TGC'GCA Cuts at: 4969 4969 Size: 6463 HaeII r_GCGC'y Cuts at: 1678 2159 2851 5610 1678 Size: 481 692 2759 2531 Fragments arranged by size: 2759 2531 692 481 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2183 2215 2362 2379 2394 Size: 32 135 32 611 32 147 17 15 Cuts at: 2394 2411 2485 2517 3495 3527 3642 3674 5104 Size: 17 74 32 978 32 115 32 1430 Cuts at: 5104 5136 5178 5210 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1430 978 611 147 135 115 74 42 32 32 32 32 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 3807 4053 669 Size: 988 281 1869 246 3079 Fragments arranged by size: 3079 1869 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6463 HpaI GTT'AAC Cuts at: 4053 4053 Size: 6463 KasI G'GCGC_C Cuts at: 2155 2155 Size: 6463 KpnI G_GTAC'C Cuts at: 2588 3788 2588 Size: 1200 5263 MfeI C'AATT_G Cuts at: 3015 4062 3015 Size: 1047 5416 MluI A'CGCG_T Cuts at: 6339 6339 Size: 6463 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2247 2641 5577 5761 1305 Size: 942 394 2936 184 2007 Fragments arranged by size: 2936 2007 942 394 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4641 Fragments arranged by size: 4641 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2438 3233 3248 3254 4421 4780 4939 Size: 648 1272 795 15 6 1167 359 159 Cuts at: 4939 518 Size: 2042 Fragments arranged by size: 2042 1272 1167 795 648 359 159 15 6 MspA1I CmG'CkG Cuts at: 1606 3879 4556 5704 5949 1606 Size: 2273 677 1148 245 2120 Fragments arranged by size: 2273 2120 1148 677 245 NarI GG'CG_CC Cuts at: 2156 2156 Size: 6463 NcoI C'CATG_G Cuts at: 513 1065 3462 513 Size: 552 2397 3514 Fragments arranged by size: 3514 2397 552 NdeI CA'TA_TG Cuts at: 387 3097 387 Size: 2710 3753 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6463 NotI GC'GGCC_GC Cuts at: 3910 3910 Size: 6463 NspI r_CATG'y Cuts at: 1132 1229 2349 3253 3509 3821 5366 1132 Size: 97 1120 904 256 312 1545 2229 Fragments arranged by size: 2229 1545 1120 904 312 256 97 PasI CC'CwG_GG Cuts at: 1348 3424 1348 Size: 2076 4387 PciI A'CATG_T Cuts at: 5362 5362 Size: 6463 PflMI CCAn_nnn'nTGG Cuts at: 1108 3260 1108 Size: 2152 4311 PfoI T'CCnGG_A Cuts at: 3525 3525 Size: 6463 PmeI GTTT'AAAC Cuts at: 3767 3767 Size: 6463 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2090 2122 4532 4564 6045 6077 2090 Size: 32 2410 32 1481 32 2476 Fragments arranged by size: 2476 2410 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6463 PsiI TTA'TAA Cuts at: 4033 4033 Size: 6463 PstI C_TGCA'G Cuts at: 830 3815 830 Size: 2985 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6463 SalI G'TCGA_C Cuts at: 1936 3805 1936 Size: 1869 4594 SbfI CC_TGCA'GG Cuts at: 3815 3815 Size: 6463 ScaI AGT'ACT Cuts at: 4711 6423 4711 Size: 1712 4751 SfcI C'TryA_G Cuts at: 826 1045 3594 3811 4946 5627 5818 826 Size: 219 2549 217 1135 681 191 1471 Fragments arranged by size: 2549 1471 1135 681 219 217 191 SfoI GGC'GCC Cuts at: 2157 2157 Size: 6463 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6463 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6463 SmaI CCC'GGG Cuts at: 1872 3623 3790 1872 Size: 1751 167 4545 Fragments arranged by size: 4545 1751 167 SmlI C'TyrA_G Cuts at: 820 1017 1943 4567 5468 5730 820 Size: 197 926 2624 901 262 1553 Fragments arranged by size: 2624 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4254 493 Size: 3761 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6463 SphI G_CATG'C Cuts at: 1132 3821 1132 Size: 2689 3774 SspI AAT'ATT Cuts at: 5 52 4387 5 Size: 47 4335 2081 Fragments arranged by size: 4335 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6463 StyI C'CwwG_G Cuts at: 513 1065 3462 3902 513 Size: 552 2397 440 3074 Fragments arranged by size: 3074 2397 552 440 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3260 3302 4490 4507 4660 4845 5290 Size: 1255 1680 42 1188 17 153 185 445 Cuts at: 5290 325 Size: 1498 Fragments arranged by size: 1680 1498 1255 1188 445 185 153 42 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2052 2631 2822 Size: 274 80 33 51 1443 75 579 191 Cuts at: 2822 4709 6421 96 Size: 1887 1712 138 Fragments arranged by size: 1887 1712 1443 579 274 191 138 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2199 2231 2859 2891 3536 3568 6423 6455 2199 Size: 32 628 32 645 32 2855 32 2207 Fragments arranged by size: 2855 2207 645 628 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6463 XbaI T'CTAG_A Cuts at: 3799 3799 Size: 6463 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3469 2011 Size: 1458 5005 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6463 XmaI C'CCGG_G Cuts at: 1870 3621 3788 1870 Size: 1751 167 4545 Fragments arranged by size: 4545 1751 167 XmnI GAAnn'nnTTC Cuts at: 3701 4592 3701 Size: 891 5572 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4271 276 Size: 53 83 186 936 2737 2468 Fragments arranged by size: 2737 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI