################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BbrPI BcgI BclI BfrI BglII BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KspI MamI MluI MroI MspA1I NaeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 861 GGTACC G^GTACC AccI 688 GTCTAC GT^[AC][GT]AC AcyI 432 GGCGCC G[GA]^CG[CT]C AflIII 533 ACGTGT A^C[GA][CT]GT AlwNI 1702 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 658 CCTCAGG CC^T[ACGT]AGG Asp700I 1974 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 861 GGTACC G^GTACC BalI 322 TGGCCA TGG^CCA BbeI 432 GGCGCC GGCGC^C BbsI 845 GAAGAC GAAGAC(2/6) BfaI 782 CTAG C^TAG BglI 559 GCCAAGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 845 GAAGAC GAAGAC(2/6) BsaHI 432 GGCGCC G[GA]^CG[CT]C BsaI 1894 GAGACC GGTCTC(1/5) BsgI 1608 CTGCAC GTGCAG(16/14) Bsp1407I 908 TGTACA T^GTACA BsrFI 1811 ACCGGC [GA]^CCGG[CT] BsrGI 908 TGTACA T^GTACA Bsu36I 658 CCTCAGG CC^T[ACGT]AGG Cfr10I 1811 ACCGGC [GA]^CCGG[CT] DraIII 17 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1739 CCATGG C^C[GA][CT]GG EarI 1046 GAAGAG CTCTTC(1/4) Eco81I 658 CCTCAGG CC^T[ACGT]AGG EcoT14I 1739 CCATGG C^C[AT][AT]GG Hin1I 432 GGCGCC G[GA]^CG[CT]C KasI 432 GGCGCC G^GCGCC KpnI 861 GGTACC GGTAC^C Ksp632I 1046 GAAGAG CTCTTC(1/4) MaeI 782 CTAG C^TAG MfeI 1292 CAATTG C^AATTG MluNI 322 TGGCCA TGG^CCA MscI 322 TGGCCA TGG^CCA MseI 964 TTAA T^TAA MunI 1292 CAATTG C^AATTG NarI 432 GGCGCC GG^CGCC NcoI 1739 CCATGG C^CATGG NdeI 1373 CATATG CA^TATG PflMI 1531 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ScaI 35 AGTACT AGT^ACT SfoI 432 GGCGCC GGC^GCC SmaI 1898 CCCGGG CCC^GGG SphI 290 GCATGC GCATG^C SspBI 908 TGTACA T^GTACA StyI 1739 CCATGG C^C[AT][AT]GG Tru9I 964 TTAA T^TAA Van91I 1531 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1739 CCATGGATGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1898 CCCGGG C^CCGGG XmaI 1898 CCCGGG C^CCGGG XmnI 1974 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AccII 414 CGCG CG^CG 676 CGCG AvaI 1898 CCCGGG C^[CT]CG[GA]G 1961 CCCGAG BanII 343 GGGCTC G[GA]GC[CT]^C 1884 GGGCTC BbvCI 1282 CCTCAGC CCTCAGC(-5/-2) 1450 CCTCAGC BlpI 700 GCTGAGC GC^T[ACGT]AGC 744 GCTGAGC BpmI 1887 CTCCAG CTGGAG(16/14) 1538 CTGGAG Bpu1102I 700 GCTGAGC GC^T[ACGT]AGC 744 GCTGAGC BsoBI 1898 CCCGGG C^[CT]CG[GA]G 1961 CCCGAG BstUI 414 CGCG CG^CG 676 CGCG CelII 700 GCTGAGC GC^T[ACGT]AGC 744 GCTGAGC EaeI 322 TGGCCA [CT]^GGCC[GA] 566 CGGCCA FauI 487 CCCGC CCCGC(4/6) 519 CCCGC HaeII 432 GGCGCC [GA]GCGC^[CT] 1124 AGCGCC HgaI 1010 GACGC GACGC(5/10) 425 GCGTC MvnI 414 CGCG CG^CG 676 CGCG SfcI 31 CTACAG C^T[GA][CT]AG 1871 CTACAG ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BanI BbvI BciVI BcnI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII