(Circular) (Six-base) MAPSORT of: pFN21AB6244.seq Check: 398 from: 1 to: 6745 pFN21AB6244 6745 bp With 182 enzymes: SgfI * July 6, 2012 17:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4555 278 Size: 53 83 186 936 3019 2468 Fragments arranged by size: 3019 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2694 4088 1937 Size: 757 1394 4594 Fragments arranged by size: 4594 1394 757 Acc65I G'GTAC_C Cuts at: 2866 4066 2866 Size: 1200 5545 AclI AA'CG_TT Cuts at: 1563 4872 5245 1563 Size: 3309 373 3063 Fragments arranged by size: 3309 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2618 3329 4808 6192 6460 1632 Size: 986 711 1479 1384 268 1917 Fragments arranged by size: 1917 1479 1384 986 711 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6548 AflIII A'CryG_T Cuts at: 1203 2538 5644 6621 1203 Size: 1335 3106 977 1327 Fragments arranged by size: 3106 1335 1327 977 AgeI A'CCGG_T Cuts at: 5576 5576 Size: 6745 AhdI GACnn_n'nnGTC Cuts at: 5474 5474 Size: 6745 AleI CACnn'nnGTG Cuts at: 1166 2720 1166 Size: 1554 5191 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2644 3548 1184 Size: 1460 904 4381 Fragments arranged by size: 4381 1460 904 AlwNI CAG_nnn'CTG Cuts at: 3712 4488 6060 3712 Size: 776 1572 4397 Fragments arranged by size: 4397 1572 776 ApaLI G'TGCA_C Cuts at: 4802 5958 4802 Size: 1156 5589 ApoI r'AATT_y Cuts at: 1493 2008 2147 2759 2897 3830 4054 4280 4432 Size: 515 139 612 138 933 224 226 152 Cuts at: 4432 6541 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 933 612 515 226 224 152 139 138 AseI AT'TA_AT Cuts at: 160 1032 5299 160 Size: 872 4267 1606 Fragments arranged by size: 4267 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6745 AvaI C'yCGr_G Cuts at: 1870 1943 3903 3966 4070 1870 Size: 73 1960 63 104 4545 Fragments arranged by size: 4545 1960 104 73 63 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3614 3647 3614 Size: 33 6712 BamHI G'GATC_C Cuts at: 4075 4442 4075 Size: 367 6378 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2437 2733 2866 3524 4066 Size: 325 217 627 650 296 133 658 542 Cuts at: 4066 5521 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 658 650 627 542 325 296 217 133 BanII G_rGCy'C Cuts at: 1391 2352 3893 4064 1391 Size: 961 1541 171 4072 Fragments arranged by size: 4072 1541 961 171 BbeI G_GCGC'C Cuts at: 2441 2441 Size: 6745 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2857 928 Size: 971 958 4816 Fragments arranged by size: 4816 971 958 BbvCI CC'TCA_GC Cuts at: 3288 3456 3288 Size: 168 6577 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4936 4970 1903 Size: 34 2999 34 3678 Fragments arranged by size: 3678 2999 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2300 3167 3642 4637 5847 2300 Size: 867 475 995 1210 3198 Fragments arranged by size: 3198 1210 995 867 475 BclI T'GATC_A Cuts at: 1548 1548 Size: 6745 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2570 5356 136 Size: 107 122 71 631 605 898 2786 1525 Fragments arranged by size: 2786 1525 898 631 605 122 107 71 BglII A'GATC_T Cuts at: 6740 6740 Size: 6745 BlpI GC'TnA_GC Cuts at: 2706 2750 4162 2706 Size: 44 1412 5289 Fragments arranged by size: 5289 1412 44 Bme1580I G_kGCm'C Cuts at: 1790 2738 3527 4806 5962 1790 Size: 948 789 1279 1156 2573 Fragments arranged by size: 2573 1279 1156 948 789 BmgBI CAC'GTC Cuts at: 1318 2539 2713 1318 Size: 1221 174 5350 Fragments arranged by size: 5350 1221 174 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2818 4476 5434 Size: 416 53 83 399 24 1401 1658 958 Cuts at: 5434 442 Size: 1753 Fragments arranged by size: 1753 1658 1401 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6745 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3757 3757 Size: 6745 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3564 3877 5405 1239 Size: 2325 313 1528 2579 Fragments arranged by size: 2579 2325 1528 313 Bpu10I CC'TnA_GC Cuts at: 3288 3456 3774 3288 Size: 168 318 6259 Fragments arranged by size: 6259 318 168 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4870 5735 6033 4870 Size: 865 298 5582 Fragments arranged by size: 5582 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3893 5408 882 Size: 617 2394 1515 2219 Fragments arranged by size: 2394 2219 1515 617 BsaAI yAC'GTr Cuts at: 493 1204 4536 493 Size: 711 3332 2702 Fragments arranged by size: 3332 2702 711 BsaBI GATnn'nnATC Cuts at: 6739 6739 Size: 6745 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2438 4552 4934 5569 Size: 53 83 186 936 905 2114 382 635 Cuts at: 5569 275 Size: 1451 Fragments arranged by size: 2114 1451 936 905 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5178 5576 5850 5997 6408 5178 Size: 398 274 147 411 5515 Fragments arranged by size: 5515 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2644 2674 3818 3848 1178 Size: 30 165 30 1241 30 1144 30 4075 Fragments arranged by size: 4075 1241 1144 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2377 2652 3826 4049 1186 Size: 1191 275 1174 223 3882 Fragments arranged by size: 3882 1191 1174 275 223 BseYI C'CCAG_C Cuts at: 2080 2459 5948 6444 2080 Size: 379 3489 496 2381 Fragments arranged by size: 3489 2381 496 379 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3598 1163 Size: 573 1862 4310 Fragments arranged by size: 4310 1862 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4195 4956 5984 1540 Size: 275 186 2194 761 1028 2301 Fragments arranged by size: 2301 2194 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2135 2561 2712 4806 4891 5962 2135 Size: 426 151 2094 85 1071 2918 Fragments arranged by size: 2918 2094 1071 426 151 85 BsmI GAATG_Cn' Cuts at: 4256 4349 4256 Size: 93 6652 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2135 2352 2561 2712 2738 3527 3893 Size: 399 345 217 209 151 26 789 366 Cuts at: 3893 4064 4806 4891 5962 1391 Size: 171 742 85 1071 2174 Fragments arranged by size: 2174 1071 789 742 399 366 345 217 209 171 151 85 26 BspEI T'CCGG_A Cuts at: 6408 6408 Size: 6745 BspHI T'CATG_A Cuts at: 4634 6364 4634 Size: 1730 5015 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4100 844 Size: 3256 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4632 1394 Size: 429 2809 3507 Fragments arranged by size: 3507 2809 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5240 5414 65 Size: 1183 3992 174 1396 Fragments arranged by size: 3992 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3816 5389 5576 1700 Size: 110 2006 1573 187 2869 Fragments arranged by size: 2869 2006 1573 187 110 BsrGI T'GTAC_A Cuts at: 96 2913 96 Size: 2817 3928 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6745 BssSI C'ACGA_G Cuts at: 4805 5817 4805 Size: 1012 5733 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6745 BstBI TT'CG_AA Cuts at: 5549 5549 Size: 6745 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6745 BstYI r'GATC_y Cuts at: 1972 2836 3033 3185 4075 4442 4827 4844 6296 Size: 864 197 152 890 367 385 17 1452 Cuts at: 6296 6382 6394 6740 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 890 864 385 367 346 197 152 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2664 2664 Size: 6745 BtgI C'CryG_G Cuts at: 513 1065 3744 513 Size: 552 2679 3514 Fragments arranged by size: 3514 2679 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6006 Fragments arranged by size: 6006 683 56 BtsI GCAGTG_nn' Cuts at: 3610 3874 4261 5055 5075 3610 Size: 264 387 794 20 5280 Fragments arranged by size: 5280 794 387 264 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6745 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6710 DraI TTT'AAA Cuts at: 4049 4049 Size: 6745 DraIII CAC_nnn'GTG Cuts at: 2027 2027 Size: 6745 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5752 809 Size: 507 4436 1802 Fragments arranged by size: 4436 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2327 2571 4192 Size: 54 1425 50 275 18 497 244 1621 Cuts at: 4192 5081 6710 8 Size: 889 1629 43 Fragments arranged by size: 1629 1621 1425 889 497 275 244 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4192 1537 Size: 275 2380 4090 Fragments arranged by size: 4090 2380 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3046 4675 1353 Size: 1693 1629 3423 Fragments arranged by size: 3423 1693 1629 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3080 3932 5332 5706 5852 387 Size: 1064 138 1491 852 1400 374 146 1280 Fragments arranged by size: 1491 1400 1280 1064 852 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2618 3329 3564 3877 4808 5405 6192 Size: 393 986 711 235 313 931 597 787 Cuts at: 6192 6460 1239 Size: 268 1524 Fragments arranged by size: 1524 986 931 787 711 597 393 313 268 235 EcoRI G'AATT_C Cuts at: 2147 2759 2897 3830 4054 4432 2147 Size: 612 138 933 224 378 4460 Fragments arranged by size: 4460 933 612 378 224 138 FspI TGC'GCA Cuts at: 5251 5251 Size: 6745 HaeII r_GCGC'y Cuts at: 1678 2441 3133 5892 1678 Size: 763 692 2759 2531 Fragments arranged by size: 2759 2531 763 692 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2228 2260 2465 2497 2644 Size: 32 135 32 656 32 205 32 147 Cuts at: 2644 2661 2676 2693 2767 2799 3777 3809 3924 Size: 17 15 17 74 32 978 32 115 Cuts at: 3924 3956 5386 5418 5460 5492 1373 Size: 32 1430 32 42 32 2626 Fragments arranged by size: 2626 1430 978 656 205 147 135 115 74 42 32 32 32 32 32 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 4089 4335 669 Size: 988 281 2151 246 3079 Fragments arranged by size: 3079 2151 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6745 HpaI GTT'AAC Cuts at: 4335 4335 Size: 6745 KasI G'GCGC_C Cuts at: 2437 2437 Size: 6745 KpnI G_GTAC'C Cuts at: 2870 4070 2870 Size: 1200 5545 MfeI C'AATT_G Cuts at: 3297 4344 3297 Size: 1047 5698 MluI A'CGCG_T Cuts at: 6621 6621 Size: 6745 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2529 2923 5859 6043 1305 Size: 1224 394 2936 184 2007 Fragments arranged by size: 2936 2007 1224 394 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2329 10 Size: 54 1425 343 497 4426 Fragments arranged by size: 4426 1425 497 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2720 3515 3530 3536 4703 5062 5221 Size: 648 1554 795 15 6 1167 359 159 Cuts at: 5221 518 Size: 2042 Fragments arranged by size: 2042 1554 1167 795 648 359 159 15 6 MspA1I CmG'CkG Cuts at: 1606 4161 4838 5986 6231 1606 Size: 2555 677 1148 245 2120 Fragments arranged by size: 2555 2120 1148 677 245 NarI GG'CG_CC Cuts at: 2438 2438 Size: 6745 NcoI C'CATG_G Cuts at: 513 1065 3744 513 Size: 552 2679 3514 Fragments arranged by size: 3514 2679 552 NdeI CA'TA_TG Cuts at: 387 3379 387 Size: 2992 3753 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6745 NotI GC'GGCC_GC Cuts at: 4192 4192 Size: 6745 NspI r_CATG'y Cuts at: 1132 1229 2299 2631 3535 3791 4103 5648 1132 Size: 97 1070 332 904 256 312 1545 2229 Fragments arranged by size: 2229 1545 1070 904 332 312 256 97 PasI CC'CwG_GG Cuts at: 1348 3706 1348 Size: 2358 4387 PciI A'CATG_T Cuts at: 5644 5644 Size: 6745 PflMI CCAn_nnn'nTGG Cuts at: 1108 3542 1108 Size: 2434 4311 PfoI T'CCnGG_A Cuts at: 3807 3807 Size: 6745 PmeI GTTT'AAAC Cuts at: 4049 4049 Size: 6745 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2372 2404 4814 4846 6327 6359 2372 Size: 32 2410 32 1481 32 2758 Fragments arranged by size: 2758 2410 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6745 PsiI TTA'TAA Cuts at: 4315 4315 Size: 6745 PstI C_TGCA'G Cuts at: 830 4097 830 Size: 3267 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6745 SalI G'TCGA_C Cuts at: 1936 4087 1936 Size: 2151 4594 SbfI CC_TGCA'GG Cuts at: 4097 4097 Size: 6745 ScaI AGT'ACT Cuts at: 2042 4993 6705 2042 Size: 2951 1712 2082 Fragments arranged by size: 2951 2082 1712 SfcI C'TryA_G Cuts at: 826 1045 2036 3876 4093 5228 5909 6100 826 Size: 219 991 1840 217 1135 681 191 1471 Fragments arranged by size: 1840 1471 1135 991 681 219 217 191 SfoI GGC'GCC Cuts at: 2439 2439 Size: 6745 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6745 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6745 SmaI CCC'GGG Cuts at: 1872 3905 4072 1872 Size: 2033 167 4545 Fragments arranged by size: 4545 2033 167 SmlI C'TyrA_G Cuts at: 820 1017 1943 4849 5750 6012 820 Size: 197 926 2906 901 262 1553 Fragments arranged by size: 2906 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4536 493 Size: 4043 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6745 SphI G_CATG'C Cuts at: 1132 2299 4103 1132 Size: 1167 1804 3774 Fragments arranged by size: 3774 1804 1167 SspI AAT'ATT Cuts at: 5 52 4669 5 Size: 47 4617 2081 Fragments arranged by size: 4617 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6745 StyI C'CwwG_G Cuts at: 513 1065 3744 4184 513 Size: 552 2679 440 3074 Fragments arranged by size: 3074 2679 552 440 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3542 3584 4772 4789 4942 5127 5572 Size: 1255 1962 42 1188 17 153 185 445 Cuts at: 5572 325 Size: 1498 Fragments arranged by size: 1962 1498 1255 1188 445 185 153 42 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2040 2334 2913 Size: 274 80 33 51 1443 63 294 579 Cuts at: 2913 3104 4991 6703 96 Size: 191 1887 1712 138 Fragments arranged by size: 1887 1712 1443 579 294 274 191 138 80 63 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2481 2513 3141 3173 3818 3850 6705 6737 2481 Size: 32 628 32 645 32 2855 32 2489 Fragments arranged by size: 2855 2489 645 628 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6745 XbaI T'CTAG_A Cuts at: 4081 4081 Size: 6745 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3751 3751 Size: 6745 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6745 XmaI C'CCGG_G Cuts at: 1870 3903 4070 1870 Size: 2033 167 4545 Fragments arranged by size: 4545 2033 167 XmnI GAAnn'nnTTC Cuts at: 3983 4874 3983 Size: 891 5854 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4553 276 Size: 53 83 186 936 3019 2468 Fragments arranged by size: 3019 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI