################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII AccII AclI AcyI AflII AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI AspEI AspHI AvaI AviII BamHI BanII BbeI BbrPI BbsI BbvCI BfrI BglI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstUI BstXI CelII Cfr10I CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoT22I FseI FspI Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 806 GGTACC G^GTACC AccI 781 GTATAC GT^[AC][GT]AC AflIII 1052 ACGTGT A^C[GA][CT]GT AlwI 571 GGATC GGATC(4/5) AlwNI 30 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 624 CCTGAGG CC^T[ACGT]AGG Aor51HI 722 AGCGCT AGC^GCT Asp718I 806 GGTACC G^GTACC AspI 616 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 319 CCTAGG C^CTAGG BalI 161 TGGCCA TGG^CCA BcgI 596 GCAGAGAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 609 TGATCA T^GATCA BcnI 432 CCGGG CC^[GC]GG BglII 332 AGATCT A^GATCT BlnI 319 CCTAGG C^CTAGG BsmAI 239 GTCTC GTCTC(1/5) Bsp1407I 743 TGTACA T^GTACA BspDI 604 ATCGAT AT^CGAT BsrGI 743 TGTACA T^GTACA BsrI 180 CCAGT ACTGG(1/-1) Bst1107I 781 GTATAC GTA^TAC BstEII 708 GGTTACC G^GT[ACGT]ACC BstPI 708 GGTTACC G^GT[ACGT]ACC BstZ17I 781 GTATAC GTA^TAC Bsu36I 624 CCTGAGG CC^T[ACGT]AGG Cac8I 1087 GCCTGC GC[ACGT]^[ACGT]GC ClaI 604 ATCGAT AT^CGAT DraI 112 TTTAAA TTT^AAA EaeI 161 TGGCCA [CT]^GGCC[GA] Eco47III 722 AGCGCT AGC^GCT Eco81I 624 CCTGAGG CC^T[ACGT]AGG EcoNI 249 CCTAGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 708 GGTTACC G^GT[ACGT]ACC EcoRI 130 GAATTC G^AATTC EcoRV 793 GATATC GAT^ATC FbaI 609 TGATCA T^GATCA HaeII 722 AGCGCT [GA]GCGC^[CT] HphI 1134 GGTGA GGTGA(8/7) KpnI 806 GGTACC GGTAC^C MaeII 1052 ACGT A^CGT MluNI 161 TGGCCA TGG^CCA MscI 161 TGGCCA TGG^CCA NciI 432 CCGGG CC^[GC]GG PflFI 616 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 961 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 147 GAGTC GAGTC(4/5) PvuII 30 CAGCTG CAG^CTG SapI 299 GCTCTTC GCTCTTC(1/4) SexAI 1007 ACCAGGT A^CC[AT]GGT SspBI 743 TGTACA T^GTACA Tth111I 616 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 961 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccIII 816 TCCGGA T^CCGGA 1202 TCCGGA AcsI 130 GAATTC [GA]^AATT[CT] 1128 GAATTT ApoI 130 GAATTC [GA]^AATT[CT] 1128 GAATTT BciVI 187 GTATCC GTATCC(6/5) 448 GTATCC BsaWI 816 TCCGGA [AT]^CCGG[AT] 1202 TCCGGA BseAI 816 TCCGGA T^CCGGA 1202 TCCGGA BspEI 816 TCCGGA T^CCGGA 1202 TCCGGA BspHI 612 TCATGA T^CATGA 989 TCATGA BspMI 1172 ACCTGC ACCTGC(4/8) 545 GCAGGT BstYI 332 AGATCT [GA]^GATC[CT] 571 GGATCT DsaI 164 CCATGG C^C[GA][CT]GG 452 CCATGG EarI 300 CTCTTC CTCTTC(1/4) 940 GAAGAG FauI 1110 CCCGC CCCGC(4/6) 568 GCGGG Ksp632I 300 CTCTTC CTCTTC(1/4) 940 GAAGAG MflI 332 AGATCT [GA]^GATC[CT] 571 GGATCT MroI 816 TCCGGA T^CCGGA 1202 TCCGGA NcoI 164 CCATGG C^CATGG 452 CCATGG PpuMI 206 AGGACCC [GA]G^G[AT]CC[CT] 770 GGGACCC RcaI 612 TCATGA T^CATGA 989 TCATGA SmlI 192 CTCAAG C^T[CT][GA]AG 886 CTCAAG StuI 246 AGGCCT AGG^CCT 269 AGGCCT TaqI 605 TCGA T^CGA 1079 TCGA Tsp45I 463 GTCAC ^GT[GC]AC 1004 GTGAC TthHB8I 605 TCGA T^CGA 1079 TCGA XhoII 332 AGATCT [GA]^GATC[CT] 571 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AfaI AluI Asp700I AvaII BanI BbvI BfaI BmyI BsaJI BsgI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp509I TspRI XmnI