(Circular) (Six-base) MAPSORT of: pFN21AE4385.seq Check: 3840 from: 1 to: 5923 pFN21AE4385 5923 bp With 182 enzymes: SgfI * March 28, 2012 14:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2541 2541 Size: 5923 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3733 278 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2787 3266 1937 Size: 850 479 4594 Fragments arranged by size: 4594 850 479 Acc65I G'GTAC_C Cuts at: 2811 3244 2811 Size: 433 5490 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2042 2603 3986 5370 5638 1632 Size: 410 561 1383 1384 268 1917 Fragments arranged by size: 1917 1384 1383 561 410 268 AfeI AGC'GCT Cuts at: 2729 2729 Size: 5923 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 3057 4822 5799 1203 Size: 1854 1765 977 1327 Fragments arranged by size: 1854 1765 1327 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5923 AlwNI CAG_nnn'CTG Cuts at: 2040 3666 5238 2040 Size: 1626 1572 2725 Fragments arranged by size: 2725 1626 1572 ApaLI G'TGCA_C Cuts at: 3980 5136 3980 Size: 1156 4767 ApoI r'AATT_y Cuts at: 1493 2135 3133 3232 3458 3610 5719 1493 Size: 642 998 99 226 152 2109 1697 Fragments arranged by size: 2109 1697 998 642 226 152 99 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 3248 1870 Size: 73 1305 4545 Fragments arranged by size: 4545 1305 73 AvrII C'CTAG_G Cuts at: 2324 2324 Size: 5923 BamHI G'GATC_C Cuts at: 3253 3620 3253 Size: 367 5556 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2644 2811 3104 3244 4699 Size: 325 217 627 857 167 293 140 1455 Cuts at: 4699 618 Size: 1842 Fragments arranged by size: 1842 1455 857 627 325 293 217 167 140 BanII G_rGCy'C Cuts at: 1391 3242 1391 Size: 1851 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4952 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2590 2624 4114 4148 1903 Size: 34 653 34 1490 34 3678 Fragments arranged by size: 3678 1490 653 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2203 2464 3815 5025 2203 Size: 261 1351 1210 3101 Fragments arranged by size: 3101 1351 1210 261 BclI T'GATC_A Cuts at: 1548 2614 1548 Size: 1066 4857 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4534 136 Size: 107 122 71 631 605 2862 1525 Fragments arranged by size: 2862 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2337 5918 2337 Size: 3581 2342 BlpI GC'TnA_GC Cuts at: 3340 3340 Size: 5923 Bme1580I G_kGCm'C Cuts at: 1790 2647 2678 3161 3984 5140 1790 Size: 857 31 483 823 1156 2573 Fragments arranged by size: 2573 1156 857 823 483 31 BmgBI CAC'GTC Cuts at: 1318 3058 1318 Size: 1740 4183 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2179 3654 4612 Size: 416 53 83 399 24 762 1475 958 Cuts at: 4612 442 Size: 1753 Fragments arranged by size: 1753 1475 958 762 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4583 1239 Size: 3344 2579 Bpu10I CC'TnA_GC Cuts at: 2354 2354 Size: 5923 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2182 2876 4048 4913 5211 2182 Size: 694 1172 865 298 2894 Fragments arranged by size: 2894 1172 865 694 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4586 882 Size: 617 3087 2219 Fragments arranged by size: 3087 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3714 493 Size: 711 2510 2702 Fragments arranged by size: 2702 2510 711 BsaBI GATnn'nnATC Cuts at: 5917 5917 Size: 5923 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3730 4112 4747 275 Size: 53 83 186 936 2197 382 635 1451 Fragments arranged by size: 2197 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2821 3207 4356 4754 5028 5175 5586 2821 Size: 386 1149 398 274 147 411 3158 Fragments arranged by size: 3158 1149 411 398 386 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5698 Fragments arranged by size: 5698 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5923 BseYI C'CCAG_C Cuts at: 2537 5126 5622 2537 Size: 2589 496 2838 Fragments arranged by size: 2838 2589 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2433 2849 3079 1163 Size: 573 697 416 230 4007 Fragments arranged by size: 4007 697 573 416 230 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3373 4134 5162 1540 Size: 275 186 1372 761 1028 2301 Fragments arranged by size: 2301 1372 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3984 4069 5140 3984 Size: 85 1071 4767 Fragments arranged by size: 4767 1071 85 BsmI GAATG_Cn' Cuts at: 3434 3527 3434 Size: 93 5830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2647 2678 3161 3242 3984 4069 5140 Size: 399 857 31 483 81 742 85 1071 Cuts at: 5140 1391 Size: 2174 Fragments arranged by size: 2174 1071 857 742 483 399 85 81 31 BspEI T'CCGG_A Cuts at: 2821 3207 5586 2821 Size: 386 2379 3158 Fragments arranged by size: 3158 2379 386 BspHI T'CATG_A Cuts at: 2617 2994 3812 5542 2617 Size: 377 818 1730 2998 Fragments arranged by size: 2998 1730 818 377 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2541 3186 3278 844 Size: 1697 645 92 3489 Fragments arranged by size: 3489 1697 645 92 BsrBI CCG'CTC Cuts at: 1394 1823 3810 1394 Size: 429 1987 3507 Fragments arranged by size: 3507 1987 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4418 4592 65 Size: 1183 3170 174 1396 Fragments arranged by size: 3170 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4567 4754 1700 Size: 110 2757 187 2869 Fragments arranged by size: 2869 2757 187 110 BsrGI T'GTAC_A Cuts at: 96 2748 96 Size: 2652 3271 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5923 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 2713 1184 Size: 1529 4394 BstYI r'GATC_y Cuts at: 1972 2337 2576 3253 3620 4005 4022 5474 5560 Size: 365 239 677 367 385 17 1452 86 Cuts at: 5560 5572 5918 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 677 385 367 365 346 239 86 17 12 BstZ17I GTA'TAC Cuts at: 2788 2788 Size: 5923 Bsu36I CC'TnA_GG Cuts at: 2630 2630 Size: 5923 BtgI C'CryG_G Cuts at: 513 1065 2169 2457 513 Size: 552 1104 288 3979 Fragments arranged by size: 3979 1104 552 288 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 3194 3217 3439 4233 4253 3194 Size: 23 222 794 20 4864 Fragments arranged by size: 4864 794 222 23 20 ClaI AT'CG_AT Cuts at: 1554 2610 1554 Size: 1056 4867 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 2119 3227 2119 Size: 1108 4815 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2166 3370 4259 Size: 54 1425 50 275 18 336 1204 889 Cuts at: 4259 5888 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1204 889 336 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3370 1537 Size: 275 1558 4090 Fragments arranged by size: 4090 1558 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2311 2940 3853 1353 Size: 958 629 913 3423 Fragments arranged by size: 3423 958 913 629 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4510 4884 5030 387 Size: 1064 138 2921 374 146 1280 Fragments arranged by size: 2921 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2603 3986 4583 5370 5638 1239 Size: 393 410 561 1383 597 787 268 1524 Fragments arranged by size: 1524 1383 787 597 561 410 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2258 2258 Size: 5923 EcoO109I rG'GnC_Cy Cuts at: 2212 2419 2776 2212 Size: 207 357 5359 Fragments arranged by size: 5359 357 207 EcoRI G'AATT_C Cuts at: 2135 3232 3610 2135 Size: 1097 378 4448 Fragments arranged by size: 4448 1097 378 EcoRV GAT'ATC Cuts at: 2800 2800 Size: 5923 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 2731 5070 1678 Size: 1053 2339 2531 Fragments arranged by size: 2531 2339 1053 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 2385 2417 2689 Size: 32 135 32 599 32 182 32 272 Cuts at: 2689 2721 4564 4596 4638 4670 1373 Size: 32 1843 32 42 32 2626 Fragments arranged by size: 2626 1843 599 272 182 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3267 3513 669 Size: 988 281 1329 246 3079 Fragments arranged by size: 3079 1329 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5923 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KpnI G_GTAC'C Cuts at: 2815 3248 2815 Size: 433 5490 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2686 5037 5221 1305 Size: 1381 2351 184 2007 Fragments arranged by size: 2351 2007 1381 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2168 10 Size: 54 1425 343 336 3765 Fragments arranged by size: 3765 1425 343 336 54 MslI CAynn'nnrTG Cuts at: 518 1166 2075 2448 2993 2996 3881 4240 4399 Size: 648 909 373 545 3 885 359 159 Cuts at: 4399 518 Size: 2042 Fragments arranged by size: 2042 909 885 648 545 373 359 159 3 MspA1I CmG'CkG Cuts at: 1606 2037 2573 3118 3339 4016 5164 5409 1606 Size: 431 536 545 221 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 545 536 431 245 221 NcoI C'CATG_G Cuts at: 513 1065 2169 2457 513 Size: 552 1104 288 3979 Fragments arranged by size: 3979 1104 552 288 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 3281 4826 1132 Size: 97 2052 1545 2229 Fragments arranged by size: 2229 2052 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5923 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 2972 1108 Size: 1864 4059 PfoI T'CCnGG_A Cuts at: 2436 2436 Size: 5923 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2212 2776 2212 Size: 564 5359 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 2829 3493 2829 Size: 664 5259 PstI C_TGCA'G Cuts at: 830 3275 830 Size: 2445 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 PvuII CAG'CTG Cuts at: 2037 2037 Size: 5923 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SanDI GG'GwC_CC Cuts at: 2776 2776 Size: 5923 SapI GCTCTTCn'nnn_ Cuts at: 2311 2311 Size: 5923 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SexAI A'CCwGG_T Cuts at: 3012 3012 Size: 5923 SfcI C'TryA_G Cuts at: 826 1045 3271 4406 5087 5278 826 Size: 219 2226 1135 681 191 1471 Fragments arranged by size: 2226 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 3250 1872 Size: 1378 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2197 2891 4027 4928 5190 820 Size: 197 926 254 694 1136 901 262 1553 Fragments arranged by size: 1553 1136 926 901 694 262 254 197 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SspI AAT'ATT Cuts at: 5 52 3847 5 Size: 47 3795 2081 Fragments arranged by size: 3795 2081 47 StuI AGG'CCT Cuts at: 1517 2253 2276 1517 Size: 736 23 5164 Fragments arranged by size: 5164 736 23 StyI C'CwwG_G Cuts at: 513 1065 2130 2169 2259 2324 2457 2514 2532 Size: 552 1065 39 90 65 133 57 18 Cuts at: 2532 2543 2906 3362 513 Size: 11 363 456 3074 Fragments arranged by size: 3074 1065 552 456 363 133 90 65 57 39 18 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2222 2662 3092 3950 3967 4120 4305 Size: 1255 642 440 430 858 17 153 185 Cuts at: 4305 4750 325 Size: 445 1498 Fragments arranged by size: 1498 1255 858 642 445 440 430 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2367 2748 4169 Size: 274 80 33 51 1443 390 381 1421 Cuts at: 4169 5881 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1421 390 381 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2461 2493 5883 5915 2461 Size: 32 3390 32 2469 Fragments arranged by size: 3390 2469 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2624 1316 Size: 1308 4615 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 3248 1870 Size: 1378 4545 XmnI GAAnn'nnTTC Cuts at: 2030 2729 2949 3080 4052 2030 Size: 699 220 131 972 3901 Fragments arranged by size: 3901 972 699 220 131 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3731 276 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SfiI SfoI SrfI SwaI XcmI