################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII AccII AccIII AclI AflII Alw44I AocI Aor51HI ApaLI AscI AspHI AspI AvaI AvaII AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI BspEI BspHI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI FbaI FseI FspI HaeII HhaI HinP1I HpaI KasI KspI MluI MroI MspA1I MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 1071 GGTACC G^GTACC AccI 1280 GTAGAC GT^[AC][GT]AC AcyI 1847 GACGCC G[GA]^CG[CT]C AgeI 676 ACCGGT A^CCGGT AhdI 649 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 305 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG AseI 1873 ATTAAT AT^TAAT AsnI 1873 ATTAAT AT^TAAT Asp700I 719 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1071 GGTACC G^GTACC AspEI 649 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 1760 GGATCC G^GATCC BsaBI 1438 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1847 GACGCC G[GA]^CG[CT]C BsaI 1021 GAGACC GGTCTC(1/5) BsaWI 676 ACCGGT [AT]^CCGG[AT] BsmFI 657 GTCCC GGGAC(10/14) BspDI 703 ATCGAT AT^CGAT BspLU11I 979 ACATGT A^CATGT BsrBI 1246 GAGCGG CCGCTC(-3/-3) BsrFI 676 ACCGGT [GA]^CCGG[CT] BstBI 584 TTCGAA TT^CGAA BstXI 1350 CCAAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1760 GGATCC [GA]^GATC[CT] Cfr10I 676 ACCGGT [GA]^CCGG[CT] ClaI 703 ATCGAT AT^CGAT DraI 100 TTTAAA TTT^AAA Eam1105I 649 GACTGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 1768 GATATC GAT^ATC EcoT22I 695 ATGCAT ATGCA^T FauI 1248 GCGGG CCCGC(4/6) HapII 677 CCGG C^CGG HgaI 1847 GACGC GACGC(5/10) Hin1I 1847 GACGCC G[GA]^CG[CT]C HindIII 862 AAGCTT A^AGCTT HpaII 677 CCGG C^CGG KpnI 1071 GGTACC GGTAC^C MamI 1438 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 599 CAATTG C^AATTG MflI 1760 GGATCC [GA]^GATC[CT] MspI 677 CCGG C^CGG MunI 599 CAATTG C^AATTG NsiI 695 ATGCAT ATGCA^T NspI 979 ACATGT [GA]CATG^[CT] NspV 584 TTCGAA TT^CGAA PinAI 676 ACCGGT A^CCGGT PshBI 1873 ATTAAT AT^TAAT SfuI 584 TTCGAA TT^CGAA SspI 947 AATATT AAT^ATT StuI 1141 AGGCCT AGG^CCT XhoII 1760 GGATCC [GA]^GATC[CT] XmnI 719 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AciI 171 CCGC CCGC(-3/-1) 1248 GCGG AflIII 206 ACACGT A^C[GA][CT]GT 979 ACATGT AlwI 1761 GATCC GGATC(4/5) 1760 GGATC ApaI 396 GGGCCC GGGCC^C 551 GGGCCC BbsI 1585 GAAGAC GAAGAC(2/6) 1620 GAAGAC BfaI 1045 CTAG C^TAG 1651 CTAG BpuAI 1585 GAAGAC GAAGAC(2/6) 1620 GAAGAC BseRI 339 GAGGAG GAGGAG(10/8) 835 GAGGAG BsgI 1232 CTGCAC GTGCAG(16/14) 1604 CTGCAC BsmI 316 GAATGC GAATGC(1/-1) 488 GCATTC Bsp1407I 685 TGTACA T^GTACA 768 TGTACA BspMI 373 ACCTGC ACCTGC(4/8) 1230 ACCTGC BsrDI 1704 CATTGC GCAATG(2/0) 83 GCAATG BsrGI 685 TGTACA T^GTACA 768 TGTACA Cac8I 937 GCTGGC GC[ACGT]^[ACGT]GC 1573 GCAAGC DraII 395 AGGGCCC [GA]G^G[ACGT]CC[CT] 396 GGGCCCT EcoO109I 395 AGGGCCC [GA]G^G[ACGT]CC[CT] 396 GGGCCCT MaeI 1045 CTAG C^TAG 1651 CTAG MaeII 208 ACGT A^CGT 1344 ACGT PleI 846 GACTC GAGTC(4/5) 1693 GACTC SapI 1243 GAAGAGC GCTCTTC(1/4) 1577 GCTCTTC ScaI 494 AGTACT AGT^ACT 1591 AGTACT SspBI 685 TGTACA T^GTACA 768 TGTACA XcmI 782 CCAAGATTTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1365 CCAAAGAGGACATGG ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AcsI AfaI AluI ApoI BalI BanI BanII BbvI BmyI BpmI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI Ksp632I MaeIII MboI MboII MluNI MnlI MscI MseI MslI MvaI MwoI NcoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I