(Circular) (Six-base) MAPSORT of: pFN21AB0402.seq Check: 6932 from: 1 to: 6637 pFN21AB0402 6637 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3244 3244 Size: 6637 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4447 278 Size: 53 83 186 936 2911 2468 Fragments arranged by size: 2911 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3286 3980 1937 Size: 1349 694 4594 Fragments arranged by size: 4594 1349 694 Acc65I G'GTAC_C Cuts at: 3076 3958 3076 Size: 882 5755 AclI AA'CG_TT Cuts at: 1563 4764 5137 1563 Size: 3201 373 3063 Fragments arranged by size: 3201 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2147 2205 3084 3609 4700 6084 6352 1632 Size: 515 58 879 525 1091 1384 268 1917 Fragments arranged by size: 1917 1384 1091 879 525 515 268 58 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6440 AflIII A'CryG_T Cuts at: 1203 2211 2984 5536 6513 1203 Size: 1008 773 2552 977 1327 Fragments arranged by size: 2552 1327 1008 977 773 AgeI A'CCGG_T Cuts at: 2681 5468 2681 Size: 2787 3850 AhdI GACnn_n'nnGTC Cuts at: 2659 5366 2659 Size: 2707 3930 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6637 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6637 AlwNI CAG_nnn'CTG Cuts at: 2315 4380 5952 2315 Size: 2065 1572 3000 Fragments arranged by size: 3000 2065 1572 ApaI G_GGCC'C Cuts at: 2405 2560 2405 Size: 155 6482 ApaLI G'TGCA_C Cuts at: 4694 5850 4694 Size: 1156 5481 ApoI r'AATT_y Cuts at: 1493 2357 2632 3093 3946 4172 4324 6433 1493 Size: 864 275 461 853 226 152 2109 1697 Fragments arranged by size: 2109 1697 864 853 461 275 226 152 AseI AT'TA_AT Cuts at: 160 1032 3879 5191 160 Size: 872 2847 1312 1606 Fragments arranged by size: 2847 1606 1312 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6637 AvaI C'yCGr_G Cuts at: 1870 1943 3962 1870 Size: 73 2019 4545 Fragments arranged by size: 4545 2019 73 BamHI G'GATC_C Cuts at: 3765 3967 4334 3765 Size: 202 367 6068 Fragments arranged by size: 6068 367 202 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3076 3232 3366 3958 5413 Size: 325 217 627 1289 156 134 592 1455 Cuts at: 5413 618 Size: 1842 Fragments arranged by size: 1842 1455 1289 627 592 325 217 156 134 BanII G_rGCy'C Cuts at: 1391 2405 2465 2478 2560 3956 1391 Size: 1014 60 13 82 1396 4072 Fragments arranged by size: 4072 1396 1014 82 60 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3597 3632 928 Size: 971 1698 35 3933 Fragments arranged by size: 3933 1698 971 35 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4828 4862 1903 Size: 34 2891 34 3678 Fragments arranged by size: 3678 2891 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4529 5739 4529 Size: 1210 5427 BclI T'GATC_A Cuts at: 1548 1548 Size: 6637 BfrBI ATG'CAT Cuts at: 2702 2702 Size: 6637 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5248 136 Size: 107 122 71 631 605 3576 1525 Fragments arranged by size: 3576 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6632 6632 Size: 6637 BlpI GC'TnA_GC Cuts at: 4054 4054 Size: 6637 Bme1580I G_kGCm'C Cuts at: 1790 2405 2560 3369 4698 5854 1790 Size: 615 155 809 1329 1156 2573 Fragments arranged by size: 2573 1329 1156 809 615 155 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6637 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2413 3131 3304 Size: 416 53 83 399 24 996 718 173 Cuts at: 3304 4368 5326 442 Size: 1064 958 1753 Fragments arranged by size: 1753 1064 996 958 718 416 399 173 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6637 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2020 2337 2376 2492 2634 2858 3135 5297 Size: 781 317 39 116 142 224 277 2162 Cuts at: 5297 1239 Size: 2579 Fragments arranged by size: 2579 2162 781 317 277 224 142 116 39 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4762 5627 5925 4762 Size: 865 298 5474 Fragments arranged by size: 5474 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3020 5300 882 Size: 617 1521 2280 2219 Fragments arranged by size: 2280 2219 1521 617 BsaAI yAC'GTr Cuts at: 493 1204 4428 493 Size: 711 3224 2702 Fragments arranged by size: 3224 2702 711 BsaBI GATnn'nnATC Cuts at: 3447 6631 3447 Size: 3184 3453 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3853 4444 4826 5461 Size: 53 83 186 936 2320 591 382 635 Cuts at: 5461 275 Size: 1451 Fragments arranged by size: 2320 1451 936 635 591 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2681 5070 5468 5742 5889 6300 2681 Size: 2389 398 274 147 411 3018 Fragments arranged by size: 3018 2389 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3818 3848 1178 Size: 30 165 30 2415 30 3967 Fragments arranged by size: 3967 2415 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2359 2855 1186 Size: 1173 496 4968 Fragments arranged by size: 4968 1173 496 BseYI C'CCAG_C Cuts at: 5840 6336 5840 Size: 496 6141 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3222 3594 1163 Size: 573 1486 372 4206 Fragments arranged by size: 4206 1486 573 372 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4087 4848 5876 1540 Size: 275 186 2086 761 1028 2301 Fragments arranged by size: 2301 2086 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4698 4783 5854 4698 Size: 85 1071 5481 Fragments arranged by size: 5481 1071 85 BsmI GAATG_Cn' Cuts at: 2327 2493 4148 4241 2327 Size: 166 1655 93 4723 Fragments arranged by size: 4723 1655 166 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2405 2465 2478 2560 3369 3956 4698 Size: 399 615 60 13 82 809 587 742 Cuts at: 4698 4783 5854 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 809 742 615 587 399 85 82 60 13 BspEI T'CCGG_A Cuts at: 6300 6300 Size: 6637 BspHI T'CATG_A Cuts at: 4526 6256 4526 Size: 1730 4907 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2387 3244 3992 844 Size: 1543 857 748 3489 Fragments arranged by size: 3489 1543 857 748 BsrBI CCG'CTC Cuts at: 1394 1823 3253 4524 1394 Size: 429 1430 1271 3507 Fragments arranged by size: 3507 1430 1271 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2095 3708 5132 5306 65 Size: 1183 847 1613 1424 174 1396 Fragments arranged by size: 1613 1424 1396 1183 847 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2681 5281 5468 1700 Size: 110 871 2600 187 2869 Fragments arranged by size: 2869 2600 871 187 110 BsrGI T'GTAC_A Cuts at: 96 2690 2773 96 Size: 2594 83 3960 Fragments arranged by size: 3960 2594 83 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6637 BssSI C'ACGA_G Cuts at: 4697 5709 4697 Size: 1012 5625 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6637 BstBI TT'CG_AA Cuts at: 2590 5441 2590 Size: 2851 3786 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6637 BstXI CCAn_nnnn'nTGG Cuts at: 3362 3362 Size: 6637 BstYI r'GATC_y Cuts at: 1972 3765 3967 4334 4719 4736 6188 6274 6286 Size: 1793 202 367 385 17 1452 86 12 Cuts at: 6286 6632 1972 Size: 346 1977 Fragments arranged by size: 1977 1793 1452 385 367 346 202 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2334 2364 3228 3331 3391 3481 3511 Size: 552 1269 30 864 103 60 90 30 Cuts at: 3511 513 Size: 3639 Fragments arranged by size: 3639 1269 864 552 103 90 60 30 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5898 Fragments arranged by size: 5898 683 56 BtsI GCAGTG_nn' Cuts at: 4153 4947 4967 4153 Size: 794 20 5823 Fragments arranged by size: 5823 794 20 ClaI AT'CG_AT Cuts at: 1554 2709 1554 Size: 1155 5482 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6602 DraI TTT'AAA Cuts at: 2107 3941 2107 Size: 1834 4803 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5644 809 Size: 507 4328 1802 Fragments arranged by size: 4328 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2970 3040 3328 Size: 54 1425 50 275 18 1140 70 288 Cuts at: 3328 3334 3388 3508 4084 4973 6602 8 Size: 6 54 120 576 889 1629 43 Fragments arranged by size: 1629 1425 1140 889 576 288 275 120 70 54 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 4084 1537 Size: 275 2272 4090 Fragments arranged by size: 4090 2272 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2336 2537 3000 3243 3589 4567 1353 Size: 983 201 463 243 346 978 3423 Fragments arranged by size: 3423 983 978 463 346 243 201 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5224 5598 5744 387 Size: 1064 138 3635 374 146 1280 Fragments arranged by size: 3635 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2020 2147 2205 2337 2376 2492 2634 Size: 393 388 127 58 132 39 116 142 Cuts at: 2634 2858 3084 3135 3609 4700 5297 6084 6352 Size: 224 226 51 474 1091 597 787 268 Cuts at: 6352 1239 Size: 1524 Fragments arranged by size: 1524 1091 787 597 474 393 388 268 226 224 142 132 127 116 58 51 39 EcoO109I rG'GnC_Cy Cuts at: 2401 2402 2401 Size: 1 6636 EcoRI G'AATT_C Cuts at: 3946 4324 3946 Size: 378 6259 EcoRV GAT'ATC Cuts at: 3775 3775 Size: 6637 FspI TGC'GCA Cuts at: 5143 5143 Size: 6637 HaeII r_GCGC'y Cuts at: 1678 5784 1678 Size: 4106 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2645 2677 2836 2868 3818 Size: 32 135 32 1073 32 159 32 950 Cuts at: 3818 3850 5278 5310 5352 5384 1373 Size: 32 1428 32 42 32 2626 Fragments arranged by size: 2626 1428 1073 950 159 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3012 3109 3116 3981 4227 669 Size: 988 281 1074 97 7 865 246 3079 Fragments arranged by size: 3079 1074 988 865 281 246 97 7 HindIII A'AGCT_T Cuts at: 748 2867 748 Size: 2119 4518 HpaI GTT'AAC Cuts at: 4227 4227 Size: 6637 KpnI G_GTAC'C Cuts at: 3080 3962 3080 Size: 882 5755 MfeI C'AATT_G Cuts at: 2604 4236 2604 Size: 1632 5005 MluI A'CGCG_T Cuts at: 6513 6513 Size: 6637 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3539 3908 5751 5935 1305 Size: 2234 369 1843 184 2007 Fragments arranged by size: 2234 2007 1843 369 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2972 3042 3330 3336 3390 Size: 54 1425 343 1140 70 288 6 54 Cuts at: 3390 3510 10 Size: 120 3137 Fragments arranged by size: 3137 1425 1140 343 288 120 70 54 54 6 MslI CAynn'nnrTG Cuts at: 518 1166 3227 3342 3348 3498 3504 3893 4595 Size: 648 2061 115 6 150 6 389 702 Cuts at: 4595 4954 5113 518 Size: 359 159 2042 Fragments arranged by size: 2061 2042 702 648 389 359 159 150 115 6 6 MspA1I CmG'CkG Cuts at: 1606 4053 4730 5878 6123 1606 Size: 2447 677 1148 245 2120 Fragments arranged by size: 2447 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3228 3331 3391 3481 513 Size: 552 2163 103 60 90 3669 Fragments arranged by size: 3669 2163 552 103 90 60 NdeI CA'TA_TG Cuts at: 387 387 Size: 6637 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6637 NotI GC'GGCC_GC Cuts at: 4084 4084 Size: 6637 NsiI A_TGCA'T Cuts at: 2704 2704 Size: 6637 NspI r_CATG'y Cuts at: 1132 1229 2988 3995 5540 1132 Size: 97 1759 1007 1545 2229 Fragments arranged by size: 2229 1759 1545 1007 97 PasI CC'CwG_GG Cuts at: 1348 3081 1348 Size: 1733 4904 PciI A'CATG_T Cuts at: 2984 5536 2984 Size: 2552 4085 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6637 PfoI T'CCnGG_A Cuts at: 3847 3847 Size: 6637 PmeI GTTT'AAAC Cuts at: 3941 3941 Size: 6637 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4706 4738 6219 6251 4706 Size: 32 1481 32 5092 Fragments arranged by size: 5092 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6637 PsiI TTA'TAA Cuts at: 4207 4207 Size: 6637 PspOMI G'GGCC_C Cuts at: 2401 2556 2401 Size: 155 6482 PstI C_TGCA'G Cuts at: 830 3989 830 Size: 3159 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6637 SalI G'TCGA_C Cuts at: 1936 3979 1936 Size: 2043 4594 SapI GCTCTTCn'nnn_ Cuts at: 3243 3589 3243 Size: 346 6291 SbfI CC_TGCA'GG Cuts at: 3989 3989 Size: 6637 ScaI AGT'ACT Cuts at: 2501 3598 4885 6597 2501 Size: 1097 1287 1712 2541 Fragments arranged by size: 2541 1712 1287 1097 SfcI C'TryA_G Cuts at: 826 1045 3985 5120 5801 5992 826 Size: 219 2940 1135 681 191 1471 Fragments arranged by size: 2940 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6637 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6637 SmaI CCC'GGG Cuts at: 1872 3964 1872 Size: 2092 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4741 5642 5904 820 Size: 197 926 2798 901 262 1553 Fragments arranged by size: 2798 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4428 493 Size: 3935 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6637 SphI G_CATG'C Cuts at: 1132 3995 1132 Size: 2863 3774 SspI AAT'ATT Cuts at: 5 52 2954 4561 5 Size: 47 2902 1607 2081 Fragments arranged by size: 2902 2081 1607 47 StuI AGG'CCT Cuts at: 1517 3148 1517 Size: 1631 5006 StyI C'CwwG_G Cuts at: 513 1065 2991 3228 3331 3355 3385 3391 3460 Size: 552 1926 237 103 24 30 6 69 Cuts at: 3460 3481 4076 513 Size: 21 595 3074 Fragments arranged by size: 3074 1926 595 552 237 103 69 30 24 21 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3029 3658 4664 4681 4834 5019 5464 Size: 1255 1449 629 1006 17 153 185 445 Cuts at: 5464 325 Size: 1498 Fragments arranged by size: 1498 1449 1255 1006 629 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2289 2499 2690 Size: 274 80 33 51 1443 312 210 191 Cuts at: 2690 2773 2929 3596 4883 6595 96 Size: 83 156 667 1287 1712 138 Fragments arranged by size: 1712 1443 1287 667 312 274 210 191 156 138 83 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6597 6629 6597 Size: 32 6605 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6637 XbaI T'CTAG_A Cuts at: 3973 3973 Size: 6637 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2794 3377 2794 Size: 583 6054 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6637 XmaI C'CCGG_G Cuts at: 1870 3962 1870 Size: 2092 4545 XmnI GAAnn'nnTTC Cuts at: 2728 4766 2728 Size: 2038 4599 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4445 276 Size: 53 83 186 936 2911 2468 Fragments arranged by size: 2911 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI