(Linear) (Six-base) MAPSORT of: KIBB0402.seq Check: 8539 from: 1 to: 1932 KIBB0402.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1239 1932 Size: 1239 693 AccI GT'mk_AC Cuts at: 0 1281 1932 Size: 1281 651 Acc65I G'GTAC_C Cuts at: 0 1071 1932 Size: 1071 861 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 200 1079 1604 1932 Size: 142 58 879 525 328 Fragments arranged by size: 879 525 328 142 58 AflIII A'CryG_T Cuts at: 0 206 979 1932 Size: 206 773 953 Fragments arranged by size: 953 773 206 AgeI A'CCGG_T Cuts at: 0 676 1932 Size: 676 1256 AhdI GACnn_n'nnGTC Cuts at: 0 654 1932 Size: 654 1278 AlwNI CAG_nnn'CTG Cuts at: 0 310 1932 Size: 310 1622 ApaI G_GGCC'C Cuts at: 0 400 555 1932 Size: 400 155 1377 Fragments arranged by size: 1377 400 155 ApoI r'AATT_y Cuts at: 0 352 627 1088 1932 Size: 352 275 461 844 Fragments arranged by size: 844 461 352 275 AseI AT'TA_AT Cuts at: 0 1874 1932 Size: 1874 58 BamHI G'GATC_C Cuts at: 0 1760 1932 Size: 1760 172 BanI G'GyrC_C Cuts at: 0 1071 1227 1361 1932 Size: 1071 156 134 571 Fragments arranged by size: 1071 571 156 134 BanII G_rGCy'C Cuts at: 0 400 460 473 555 1932 Size: 400 60 13 82 1377 Fragments arranged by size: 1377 400 82 60 13 BbsI GAAGACnn'nnnn_ Cuts at: 0 1592 1627 1932 Size: 1592 35 305 Fragments arranged by size: 1592 305 35 BfrBI ATG'CAT Cuts at: 0 697 1932 Size: 697 1235 Bme1580I G_kGCm'C Cuts at: 0 400 555 1364 1932 Size: 400 155 809 568 Fragments arranged by size: 809 568 400 155 BmrI ACTGGGnnnn_n' Cuts at: 0 408 1126 1299 1932 Size: 408 718 173 633 Fragments arranged by size: 718 633 408 173 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 15 332 371 487 629 853 1130 1932 Size: 15 317 39 116 142 224 277 802 Fragments arranged by size: 802 317 277 224 142 116 39 15 BsaI GGTCTCn'nnnn_ Cuts at: 0 1015 1932 Size: 1015 917 BsaBI GATnn'nnATC Cuts at: 0 1442 1932 Size: 1442 490 BsaHI Gr'CG_yC Cuts at: 0 1848 1932 Size: 1848 84 BsaWI w'CCGG_w Cuts at: 0 676 1932 Size: 676 1256 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1813 1843 1932 Size: 1813 30 89 Fragments arranged by size: 1813 89 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 354 850 1932 Size: 354 496 1082 Fragments arranged by size: 1082 496 354 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1217 1589 1932 Size: 1217 372 343 Fragments arranged by size: 1217 372 343 BsmI GAATG_Cn' Cuts at: 0 322 488 1932 Size: 322 166 1444 Fragments arranged by size: 1444 322 166 Bsp1286I G_dGCh'C Cuts at: 0 400 460 473 555 1364 1932 Size: 400 60 13 82 809 568 Fragments arranged by size: 809 568 400 82 60 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 382 1239 1932 Size: 382 857 693 Fragments arranged by size: 857 693 382 BsrBI CCG'CTC Cuts at: 0 1248 1932 Size: 1248 684 BsrDI GCAATG_nn' Cuts at: 0 90 1703 1932 Size: 90 1613 229 Fragments arranged by size: 1613 229 90 BsrFI r'CCGG_y Cuts at: 0 676 1932 Size: 676 1256 BsrGI T'GTAC_A Cuts at: 0 685 768 1932 Size: 685 83 1164 Fragments arranged by size: 1164 685 83 BstBI TT'CG_AA Cuts at: 0 585 1932 Size: 585 1347 BstXI CCAn_nnnn'nTGG Cuts at: 0 1357 1932 Size: 1357 575 BstYI r'GATC_y Cuts at: 0 1760 1932 Size: 1760 172 BtgI C'CryG_G Cuts at: 0 329 359 1223 1326 1386 1476 1506 1932 Size: 329 30 864 103 60 90 30 426 Fragments arranged by size: 864 426 329 103 90 60 30 30 ClaI AT'CG_AT Cuts at: 0 704 1932 Size: 704 1228 DraI TTT'AAA Cuts at: 0 102 1932 Size: 102 1830 EaeI y'GGCC_r Cuts at: 0 965 1035 1323 1329 1383 1503 1932 Size: 965 70 288 6 54 120 429 Fragments arranged by size: 965 429 288 120 70 54 6 EarI CTCTTCn'nnn_ Cuts at: 0 331 532 995 1238 1584 1932 Size: 331 201 463 243 346 348 Fragments arranged by size: 463 348 346 331 243 201 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 15 142 200 332 371 487 629 853 Size: 15 127 58 132 39 116 142 224 Cuts at: 853 1079 1130 1604 1932 Size: 226 51 474 328 Fragments arranged by size: 474 328 226 224 142 132 127 116 58 51 39 15 EcoO109I rG'GnC_Cy Cuts at: 0 396 397 1932 Size: 396 1 1535 Fragments arranged by size: 1535 396 1 EcoRV GAT'ATC Cuts at: 0 1770 1932 Size: 1770 162 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 640 672 831 863 1813 1845 1932 Size: 640 32 159 32 950 32 87 Fragments arranged by size: 950 640 159 87 32 32 32 HincII GTy'rAC Cuts at: 0 1007 1104 1111 1932 Size: 1007 97 7 821 Fragments arranged by size: 1007 821 97 7 HindIII A'AGCT_T Cuts at: 0 862 1932 Size: 862 1070 KpnI G_GTAC'C Cuts at: 0 1075 1932 Size: 1075 857 MfeI C'AATT_G Cuts at: 0 599 1932 Size: 599 1333 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1534 1903 1932 Size: 1534 369 29 Fragments arranged by size: 1534 369 29 MscI TGG'CCA Cuts at: 0 967 1037 1325 1331 1385 1505 1932 Size: 967 70 288 6 54 120 427 Fragments arranged by size: 967 427 288 120 70 54 6 MslI CAynn'nnrTG Cuts at: 0 1222 1337 1343 1493 1499 1888 1932 Size: 1222 115 6 150 6 389 44 Fragments arranged by size: 1222 389 150 115 44 6 6 NcoI C'CATG_G Cuts at: 0 1223 1326 1386 1476 1932 Size: 1223 103 60 90 456 Fragments arranged by size: 1223 456 103 90 60 NsiI A_TGCA'T Cuts at: 0 699 1932 Size: 699 1233 NspI r_CATG'y Cuts at: 0 983 1932 Size: 983 949 PasI CC'CwG_GG Cuts at: 0 1076 1932 Size: 1076 856 PciI A'CATG_T Cuts at: 0 979 1932 Size: 979 953 PfoI T'CCnGG_A Cuts at: 0 1842 1932 Size: 1842 90 PspOMI G'GGCC_C Cuts at: 0 396 551 1932 Size: 396 155 1381 Fragments arranged by size: 1381 396 155 SapI GCTCTTCn'nnn_ Cuts at: 0 1238 1584 1932 Size: 1238 346 348 Fragments arranged by size: 1238 348 346 ScaI AGT'ACT Cuts at: 0 496 1593 1932 Size: 496 1097 339 Fragments arranged by size: 1097 496 339 SspI AAT'ATT Cuts at: 0 949 1932 Size: 949 983 StuI AGG'CCT Cuts at: 0 1143 1932 Size: 1143 789 StyI C'CwwG_G Cuts at: 0 986 1223 1326 1350 1380 1386 1455 1476 Size: 986 237 103 24 30 6 69 21 Cuts at: 1476 1932 Size: 456 Fragments arranged by size: 986 456 237 103 69 30 24 21 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1024 1653 1932 Size: 1024 629 279 Fragments arranged by size: 1024 629 279 TatI w'GTAC_w Cuts at: 0 284 494 685 768 924 1591 1932 Size: 284 210 191 83 156 667 341 Fragments arranged by size: 667 341 284 210 191 156 83 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 789 1372 1932 Size: 789 583 560 Fragments arranged by size: 789 583 560 XmnI GAAnn'nnTTC Cuts at: 0 723 1932 Size: 723 1209 Enzymes that do cut: AarI AccI Acc65I AcuI AflIII AgeI AhdI AlwNI ApaI ApoI AseI BamHI BanI BanII BbsI BfrBI Bme1580I BmrI BpmI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstBI BstXI BstYI BtgI ClaI DraI EaeI EarI Eco57MI EcoO109I EcoRV Hin4I HincII HindIII KpnI MfeI MmeI MscI MslI NcoI NsiI NspI PasI PciI PfoI PspOMI SapI ScaI SspI StuI StyI TaqII TatI XcmI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AleI AlfI AloI ApaLI AscI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaAI BseYI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I BtgZI BtsI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HaeII HpaI KasI MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI TstI Tth111I XbaI XhoI XmaI ZraI