Restriction map for
pFN21AE5273
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanII
BbeI BbrPI BciVI BclI BcnI
BfrI BglI BlnI BlpI BmyI
Bpu1102I BsaAI BsaHI BsaI BseAI
BseRI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII CfoI Cfr10I CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HphI KasI KpnI Ksp632I KspI
MaeII MaeIII MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI Tsp45I TspRI Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 42 AAATTT [GA]^AATT[CT]
AflIII 173 ACATGT A^C[GA][CT]GT
AlwI 378 GATCC GGATC(4/5)
AlwNI 240 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 42 AAATTT [GA]^AATT[CT]
BanI 133 GGCACC G^G[CT][GA]CC
BbsI 34 GAAGAC GAAGAC(2/6)
BbvCI 237 CCTCAGC CCTCAGC(-5/-2)
BcgI 483 CGATTGGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 266 CTAG C^TAG
BglII 290 AGATCT A^GATCT
BpmI 397 CTGGAG CTGGAG(16/14)
BpuAI 34 GAAGAC GAAGAC(2/6)
BsaBI 285 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 470 ACCGGA [AT]^CCGG[AT]
BsiEI 231 CGACCG CG[GA][CT]^CG
Bsp1407I 176 TGTACA T^GTACA
BspDI 481 ATCGAT AT^CGAT
BspLU11I 173 ACATGT A^CATGT
BsrGI 176 TGTACA T^GTACA
BstF5I 218 GGATG GGATG(2/0)
BstXI 116 CCATTAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 440 GCAGGC GC[ACGT]^[ACGT]GC
ClaI 481 ATCGAT AT^CGAT
DsaI 435 CCGTGG C^C[GA][CT]GG
EcoNI 265 CCTAGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 218 GGATG GGATG(9/13)
HapII 471 CCGG C^CGG
HinfI 285 GATTC G^A[ACGT]TC
HpaII 471 CCGG C^CGG
MaeI 266 CTAG C^TAG
MamI 285 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 119 TTAA T^TAA
MslI 117 CATTAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 503 CAGCTG C[AC]G^C[GT]G
MspI 471 CCGG C^CGG
MwoI 496 GCCTCCCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 479 CCATCGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 246 CTGCAG CTGCA^G
PvuII 503 CAGCTG CAG^CTG
SfcI 246 CTGCAG C^T[GA][CT]AG
SspBI 176 TGTACA T^GTACA
TaqI 482 TCGA T^CGA
TfiI 285 GATTC G^A[AT]TC
Tru9I 119 TTAA T^TAA
TthHB8I 482 TCGA T^CGA
Van91I 479 CCATCGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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BbvI 493 GCAGC GCAGC(8/12)
107 GCTGC
BsaJI 137 CCTGGG C^C[ACGT][ACGT]GG
435 CCGTGG
BsgI 80 GTGCAG GTGCAG(16/14)
491 GTGCAG
BssKI 137 CCTGG ^CC[ACGT]GG
453 CCTGG
BstNI 137 CCTGG CC^[AT]GG
453 CCTGG
BstYI 290 AGATCT [GA]^GATC[CT]
377 AGATCC
Cfr13I 155 GGCCC G^G[ACGT]CC
222 GGGCC
DdeI 100 CTAAG C^T[ACGT]AG
238 CTCAG
DpnII 291 GATC ^GATC
378 GATC
EcoRII 137 CCTGG ^CC[AT]GG
453 CCTGG
MboI 291 GATC ^GATC
378 GATC
MboII 34 GAAGA GAAGA(8/7)
337 GAAGA
MflI 290 AGATCT [GA]^GATC[CT]
377 AGATCC
MvaI 137 CCTGG CC^[AT]GG
453 CCTGG
NdeII 291 GATC ^GATC
378 GATC
NlaIV 13 GGAACC GG[ACGT]^[ACGT]CC
133 GGCACC
NspI 173 ACATGT [GA]CATG^[CT]
368 ACATGC
Sau3AI 291 GATC ^GATC
378 GATC
Sau96I 155 GGCCC G^G[ACGT]CC
222 GGGCC
ScrFI 137 CCTGG CC^[ACGT]GG
453 CCTGG
TseI 107 GCTGC G^C[AT]GC
493 GCAGC
Tsp509I 43 AATT ^AATT
362 AATT
XhoII 290 AGATCT [GA]^GATC[CT]
377 AGATCC
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# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
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AciI AfaI AluI BsiYI BslI
BsrI Fnu4HI HaeIII ItaI MnlI
NlaIII RsaI