Restriction map for
pFN21AE5273
(Circular) (Six-base) MAPSORT of: pFN21AE5273.seq Check: 3276 from: 1 to: 5239
pFN21AE5273 5239 bp
With 182 enzymes: SgfI *
January 30, 2014 17:16 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3049 278
Size: 53 83 186 936 1513 2468
Fragments arranged by size:
2468 1513 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2582 1937
Size: 645 4594
Acc65I G'GTAC_C
Cuts at: 2560 2560
Size: 5239
AclI AA'CG_TT
Cuts at: 1563 3366 3739 1563
Size: 1803 373 3063
Fragments arranged by size:
3063 1803 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3302 4686 4954 1632
Size: 1670 1384 268 1917
Fragments arranged by size:
1917 1670 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5042
AflIII A'CryG_T
Cuts at: 1203 2178 4138 5115 1203
Size: 975 1960 977 1327
Fragments arranged by size:
1960 1327 977 975
AgeI A'CCGG_T
Cuts at: 4070 4070
Size: 5239
AhdI GACnn_n'nnGTC
Cuts at: 3968 3968
Size: 5239
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5239
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5239
AlwNI CAG_nnn'CTG
Cuts at: 2250 2982 4554 2250
Size: 732 1572 2935
Fragments arranged by size:
2935 1572 732
ApaLI G'TGCA_C
Cuts at: 3296 4452 3296
Size: 1156 4083
ApoI r'AATT_y
Cuts at: 1493 2047 2548 2774 2926 5035 1493
Size: 554 501 226 152 2109 1697
Fragments arranged by size:
2109 1697 554 501 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3793 160
Size: 872 2761 1606
Fragments arranged by size:
2761 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5239
AvaI C'yCGr_G
Cuts at: 1870 1943 2564 1870
Size: 73 621 4545
Fragments arranged by size:
4545 621 73
BamHI G'GATC_C
Cuts at: 2569 2936 2569
Size: 367 4872
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2138 2560 4015 618
Size: 325 217 627 351 422 1455 1842
Fragments arranged by size:
1842 1455 627 422 351 325 217
BanII G_rGCy'C
Cuts at: 1391 2558 1391
Size: 1167 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2046 928
Size: 971 147 4121
Fragments arranged by size:
4121 971 147
BbvCI CC'TCA_GC
Cuts at: 2243 2243
Size: 5239
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2477 2511 3430 3464 1903
Size: 34 540 34 919 34 3678
Fragments arranged by size:
3678 919 540 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3131 4341 3131
Size: 1210 4029
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5239
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3850 136
Size: 107 122 71 631 605 2178 1525
Fragments arranged by size:
2178 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2295 5234 2295
Size: 2939 2300
BlpI GC'TnA_GC
Cuts at: 2656 2656
Size: 5239
Bme1580I G_kGCm'C
Cuts at: 1790 3300 4456 1790
Size: 1510 1156 2573
Fragments arranged by size:
2573 1510 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5239
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2970 3928 442
Size: 416 53 83 399 24 1553 958 1753
Fragments arranged by size:
1753 1553 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5239
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 5239
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2423 3899 1239
Size: 1184 1476 2579
Fragments arranged by size:
2579 1476 1184
Bpu10I CC'TnA_GC
Cuts at: 2243 2243
Size: 5239
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3364 4229 4527 3364
Size: 865 298 4076
Fragments arranged by size:
4076 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3902 882
Size: 617 2403 2219
Fragments arranged by size:
2403 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3030 493
Size: 711 1826 2702
Fragments arranged by size:
2702 1826 711
BsaBI GATnn'nnATC
Cuts at: 2294 5233 2294
Size: 2939 2300
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3046 3428 4063 275
Size: 53 83 186 936 1513 382 635 1451
Fragments arranged by size:
1513 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2475 3672 4070 4344 4491 4902 2475
Size: 1197 398 274 147 411 2812
Fragments arranged by size:
2812 1197 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5014
Fragments arranged by size:
5014 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5239
BseYI C'CCAG_C
Cuts at: 2171 2506 4442 4938 2171
Size: 335 1936 496 2472
Fragments arranged by size:
2472 1936 496 335
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2106 2517 1163
Size: 573 370 411 3885
Fragments arranged by size:
3885 573 411 370
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2239 2689 3450 4478 1540
Size: 275 186 238 450 761 1028 2301
Fragments arranged by size:
2301 1028 761 450 275 238 186
BsiHKAI G_wGCw'C
Cuts at: 3300 3385 4456 3300
Size: 85 1071 4083
Fragments arranged by size:
4083 1071 85
BsmI GAATG_Cn'
Cuts at: 2750 2843 2750
Size: 93 5146
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2558 3300 3385 4456 1391
Size: 399 768 742 85 1071 2174
Fragments arranged by size:
2174 1071 768 742 399 85
BspEI T'CCGG_A
Cuts at: 4902 4902
Size: 5239
BspHI T'CATG_A
Cuts at: 3128 4858 3128
Size: 1730 3509
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2594 844
Size: 1750 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3126 1394
Size: 429 1303 3507
Fragments arranged by size:
3507 1303 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3734 3908 65
Size: 1183 2486 174 1396
Fragments arranged by size:
2486 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3883 4070 1700
Size: 110 2073 187 2869
Fragments arranged by size:
2869 2073 187 110
BsrGI T'GTAC_A
Cuts at: 96 2181 96
Size: 2085 3154
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5239
BssSI C'ACGA_G
Cuts at: 3299 4311 3299
Size: 1012 4227
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5239
BstBI TT'CG_AA
Cuts at: 4043 4043
Size: 5239
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5239
BstXI CCAn_nnnn'nTGG
Cuts at: 2128 2128
Size: 5239
BstYI r'GATC_y
Cuts at: 1972 2295 2382 2569 2936 3321 3338 4790 4876
Size: 323 87 187 367 385 17 1452 86
Cuts at: 4876 4888 5234 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 323 187 87
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2440 513
Size: 552 1375 3312
Fragments arranged by size:
3312 1375 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4500
Fragments arranged by size:
4500 683 56
BtsI GCAGTG_nn'
Cuts at: 2755 3549 3569 2755
Size: 794 20 4425
Fragments arranged by size:
4425 794 20
ClaI AT'CG_AT
Cuts at: 1554 2487 1554
Size: 933 4306
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2422 2457 529
Size: 35 1858 35 3311
Fragments arranged by size:
3311 1858 35 35
DraI TTT'AAA
Cuts at: 2543 2543
Size: 5239
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4246 809
Size: 507 2930 1802
Fragments arranged by size:
2930 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2686 3575 5204
Size: 54 1425 50 275 18 856 889 1629
Cuts at: 5204 8
Size: 43
Fragments arranged by size:
1629 1425 889 856 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2686 1537
Size: 275 874 4090
Fragments arranged by size:
4090 874 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3169 1353
Size: 1816 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2513 3826 4200 4346 387
Size: 1064 138 924 1313 374 146 1280
Fragments arranged by size:
1313 1280 1064 924 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2423 3302 3899 4686 4954 1239
Size: 393 791 879 597 787 268 1524
Fragments arranged by size:
1524 879 791 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2274 2274
Size: 5239
EcoRI G'AATT_C
Cuts at: 2548 2926 2548
Size: 378 4861
FspI TGC'GCA
Cuts at: 3745 3745
Size: 5239
HaeII r_GCGC'y
Cuts at: 1678 4386 1678
Size: 2708 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3880 3912 3954 3986 1373
Size: 32 135 32 2308 32 42 32 2626
Fragments arranged by size:
2626 2308 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2583 2829 669
Size: 988 281 645 246 3079
Fragments arranged by size:
3079 988 645 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5239
HpaI GTT'AAC
Cuts at: 2829 2829
Size: 5239
KpnI G_GTAC'C
Cuts at: 2564 2564
Size: 5239
MfeI C'AATT_G
Cuts at: 2838 2838
Size: 5239
MluI A'CGCG_T
Cuts at: 5115 5115
Size: 5239
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2201 4353 4537 1305
Size: 896 2152 184 2007
Fragments arranged by size:
2152 2007 896 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3417
Fragments arranged by size:
3417 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2126 3197 3556 3715 518
Size: 648 960 1071 359 159 2042
Fragments arranged by size:
2042 1071 960 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2510 2655 3332 4480 4725 1606
Size: 904 145 677 1148 245 2120
Fragments arranged by size:
2120 1148 904 677 245 145
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4687
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5239
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5239
NotI GC'GGCC_GC
Cuts at: 2686 2686
Size: 5239
NspI r_CATG'y
Cuts at: 1132 1229 2182 2377 2597 4142 1132
Size: 97 953 195 220 1545 2229
Fragments arranged by size:
2229 1545 953 220 195 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5239
PciI A'CATG_T
Cuts at: 2178 4138 2178
Size: 1960 3279
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2490 1108
Size: 1382 3857
PfoI T'CCnGG_A
Cuts at: 2457 2457
Size: 5239
PmeI GTTT'AAAC
Cuts at: 2543 2543
Size: 5239
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3308 3340 4821 4853 3308
Size: 32 1481 32 3694
Fragments arranged by size:
3694 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5239
PsiI TTA'TAA
Cuts at: 2809 2809
Size: 5239
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2011 2043 2011
Size: 32 5207
PstI C_TGCA'G
Cuts at: 830 2255 2591 830
Size: 1425 336 3478
Fragments arranged by size:
3478 1425 336
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5239
PvuII CAG'CTG
Cuts at: 2510 2510
Size: 5239
SalI G'TCGA_C
Cuts at: 1936 2581 1936
Size: 645 4594
SbfI CC_TGCA'GG
Cuts at: 2591 2591
Size: 5239
ScaI AGT'ACT
Cuts at: 3487 5199 3487
Size: 1712 3527
SfcI C'TryA_G
Cuts at: 826 1045 2251 2587 3722 4403 4594 826
Size: 219 1206 336 1135 681 191 1471
Fragments arranged by size:
1471 1206 1135 681 336 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5239
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5239
SmaI CCC'GGG
Cuts at: 1872 2566 1872
Size: 694 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3343 4244 4506 820
Size: 197 926 1400 901 262 1553
Fragments arranged by size:
1553 1400 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3030 493
Size: 2537 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5239
SphI G_CATG'C
Cuts at: 1132 2597 1132
Size: 1465 3774
SspI AAT'ATT
Cuts at: 5 52 3163 5
Size: 47 3111 2081
Fragments arranged by size:
3111 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5239
StyI C'CwwG_G
Cuts at: 513 1065 2678 513
Size: 552 1613 3074
Fragments arranged by size:
3074 1613 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3266 3283 3436 3621 4066 325
Size: 1255 1686 17 153 185 445 1498
Fragments arranged by size:
1686 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 3485 5197
Size: 274 80 33 51 1443 204 1304 1712
Cuts at: 5197 96
Size: 138
Fragments arranged by size:
1712 1443 1304 274 204 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5199 5231 5199
Size: 32 5207
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5239
XbaI T'CTAG_A
Cuts at: 2575 2575
Size: 5239
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5239
XmaI C'CCGG_G
Cuts at: 1870 2564 1870
Size: 694 4545
XmnI GAAnn'nnTTC
Cuts at: 3368 3368
Size: 5239
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3047 276
Size: 53 83 186 936 1513 2468
Fragments arranged by size:
2468 1513 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI